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The map label for this gene is cobA [H]

Identifier: 78780172

GI number: 78780172

Start: 1679552

End: 1680355

Strand: Reverse

Name: cobA [H]

Synonym: PMT9312_1787

Alternate gene names: 78780172

Gene position: 1680355-1679552 (Counterclockwise)

Preceding gene: 78780177

Following gene: 78780161

Centisome position: 98.31

GC content: 32.46

Gene sequence:

>804_bases
GTGCCTGGCATTGTTTATTTAGTTGGAGCAGGTCCTGGTGACCCTGAGCTTTTAACTTTAAAAGCTTTACGTCTAATAAA
AAATTGTGATGCATTAGTTCATGATGCTTTAATCCCGGATGAAATAACAAAAGAGGCAGGAAAAAATACAGAAATTTACC
ATGTAGGTAAAAGAGCGGGGAAGTGTTCTGTACCTCAGATTGAAACTAATGCTCTTATTTTGAAATTGTCAAAAGAAGGC
AAAAATGTTGTAAGGCTTAAAGGAGGAGATCCATTTGTCTTTTCTAGGGGTGGTGAAGAAGTATCGTTTTTAGAAAAAAA
TGGAGTTTCAGTTGAAATAGTTCCTGGGATTACTTCTGGTATAGCTGCTCCCACATATTTTGGTATTCCACTTACCCATA
GAGATGCTGCGAGTTCTGTAACTTTCGTCACTGGCCATGAGCATGTTGATAAGGATAAAAAGAGCGTCAATTGGAGAGAT
TTAGCTAAATCTTCAGATAGCTTAGTTATTTTTATGGGTATAAAAAATATTGAATTTATTGTAGAGGAATTAATTTTAGG
CGGCTTATGTAAGAATACAAAATGCGCTGTAATTCAGGAAGCTACTTTGAAAAATCAAAAATGTTTTATAGAGAAATTAG
ATAATCTCGTAGATAAAATCAAAGATAAAGAATTTTTAGCTCCATCAATTATCATTATTGGAAAAATTGTTGAATATAAA
GTCAATAACAATATCACTAAAGTATCTGATGTCTATTTACCAGATATTAATAAAGTTCAACTATATAATAAATCCCAAAA
GTAA

Upstream 100 bases:

>100_bases
GGAGAGTAAATTTTTTATTTGGTTTTAATAAACTTTCTTTCACAATATTTACCTTCATTACGATTAACTTCTACTACCAT
ACTTGTTAATGGAGATTATT

Downstream 100 bases:

>100_bases
ATTGGATCGAATTTAGGTTGAAGCTTTAAATCATTAACTTGATTAATTTGTCTGCAAGATAAGCTATTTATTGCAACTAT
TACGTCATCATTTTGAAATT

Product: uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MPGIVYLVGAGPGDPELLTLKALRLIKNCDALVHDALIPDEITKEAGKNTEIYHVGKRAGKCSVPQIETNALILKLSKEG
KNVVRLKGGDPFVFSRGGEEVSFLEKNGVSVEIVPGITSGIAAPTYFGIPLTHRDAASSVTFVTGHEHVDKDKKSVNWRD
LAKSSDSLVIFMGIKNIEFIVEELILGGLCKNTKCAVIQEATLKNQKCFIEKLDNLVDKIKDKEFLAPSIIIIGKIVEYK
VNNNITKVSDVYLPDINKVQLYNKSQK

Sequences:

>Translated_267_residues
MPGIVYLVGAGPGDPELLTLKALRLIKNCDALVHDALIPDEITKEAGKNTEIYHVGKRAGKCSVPQIETNALILKLSKEG
KNVVRLKGGDPFVFSRGGEEVSFLEKNGVSVEIVPGITSGIAAPTYFGIPLTHRDAASSVTFVTGHEHVDKDKKSVNWRD
LAKSSDSLVIFMGIKNIEFIVEELILGGLCKNTKCAVIQEATLKNQKCFIEKLDNLVDKIKDKEFLAPSIIIIGKIVEYK
VNNNITKVSDVYLPDINKVQLYNKSQK
>Mature_266_residues
PGIVYLVGAGPGDPELLTLKALRLIKNCDALVHDALIPDEITKEAGKNTEIYHVGKRAGKCSVPQIETNALILKLSKEGK
NVVRLKGGDPFVFSRGGEEVSFLEKNGVSVEIVPGITSGIAAPTYFGIPLTHRDAASSVTFVTGHEHVDKDKKSVNWRDL
AKSSDSLVIFMGIKNIEFIVEELILGGLCKNTKCAVIQEATLKNQKCFIEKLDNLVDKIKDKEFLAPSIIIIGKIVEYKV
NNNITKVSDVYLPDINKVQLYNKSQK

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=238, Percent_Identity=41.5966386554622, Blast_Score=183, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6322922, Length=243, Percent_Identity=29.2181069958848, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 29258; Mature: 29127

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGIVYLVGAGPGDPELLTLKALRLIKNCDALVHDALIPDEITKEAGKNTEIYHVGKRAG
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCC
KCSVPQIETNALILKLSKEGKNVVRLKGGDPFVFSRGGEEVSFLEKNGVSVEIVPGITSG
CCCCCEEECCEEEEEEECCCCEEEEEECCCCEEECCCCCEEEEEECCCCEEEEECCCCCC
IAAPTYFGIPLTHRDAASSVTFVTGHEHVDKDKKSVNWRDLAKSSDSLVIFMGIKNIEFI
CCCCEEECCCEECCCCCCCEEEEECCHHHCCHHHCCCHHHHHCCCCCEEEEEECCHHHHH
VEELILGGLCKNTKCAVIQEATLKNQKCFIEKLDNLVDKIKDKEFLAPSIIIIGKIVEYK
HHHHHHCCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHCCCHHCCCEEEEEEEEEEEE
VNNNITKVSDVYLPDINKVQLYNKSQK
ECCCCEEEEEEECCCCCEEEEECCCCC
>Mature Secondary Structure 
PGIVYLVGAGPGDPELLTLKALRLIKNCDALVHDALIPDEITKEAGKNTEIYHVGKRAG
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCC
KCSVPQIETNALILKLSKEGKNVVRLKGGDPFVFSRGGEEVSFLEKNGVSVEIVPGITSG
CCCCCEEECCEEEEEEECCCCEEEEEECCCCEEECCCCCEEEEEECCCCEEEEECCCCCC
IAAPTYFGIPLTHRDAASSVTFVTGHEHVDKDKKSVNWRDLAKSSDSLVIFMGIKNIEFI
CCCCEEECCCEECCCCCCCEEEEECCHHHCCHHHCCCHHHHHCCCCCEEEEEECCHHHHH
VEELILGGLCKNTKCAVIQEATLKNQKCFIEKLDNLVDKIKDKEFLAPSIIIIGKIVEYK
HHHHHHCCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHCCCHHCCCEEEEEEEEEEEE
VNNNITKVSDVYLPDINKVQLYNKSQK
ECCCCEEEEEEECCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]