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The map label for this gene is dnaB [H]

Identifier: 78780151

GI number: 78780151

Start: 1657549

End: 1658931

Strand: Direct

Name: dnaB [H]

Synonym: PMT9312_1766

Alternate gene names: 78780151

Gene position: 1657549-1658931 (Clockwise)

Preceding gene: 78780150

Following gene: 78780152

Centisome position: 96.98

GC content: 35.21

Gene sequence:

>1383_bases
ATGGTTTCCGTACCTTTTCCAAATAATGGGCAAAATAAAAATTTTAAAAAGGATTTTAATAGTGAAAATTCTGGATTAGT
GCCTCCTCAAAACGTTCAAGCAGAGGAAGCTGTTCTTGGTGGCATACTTCTTGATCCAGACGCGATCGGAAGAATTGCAG
ACTTAATTAAACCTGAAGCTTTTTATATAAATGCCCATCAAGAGATTTATAGAACAGCATTAATGCTGCATAGCCAGGGT
AAACCAACTGATTTAACATCAATGAGTGCTTGGTTAGCAGATAATGGATCACTAGAAAAAATTGGAGGGAACAGCAAACT
GGTAGAACTAGTTGAAAATGTTTCATCTACAGCTTCCATAGAACAAGTTGCTAATTTAATTAACGACAAATTCATGAGAA
GGCAGCTTATCAGATCTGGAAATGAGGTGGTTCAACTAGGTTTTGATCAGACTCAAGATACTAATGAAGTTTTAGATAAA
GCGGAGCAAAAAATATTTGAAATCAGTCAAGAAAAACCTTCAAAAGGTCTTACTCAAGCAGCTGAAATCCTCACAAGCAC
TTTCAATGAAATAGAGTCGAGATCATTAGGTACTTCAGTAGCTGGAATTCCTGTAAATTTTTACGACCTTGATGCGATGA
CTCAAGGTTTTCAAAGAAGTGATTTGATAATTGTTGCTGGCAGACCTTCAATGGGAAAAACTTCAATAGTTCTTAATCTG
GCTAAAAATGTTGCACAATCTCAAGATTTACCTGTTTGCGTATTTAGCCTTGAAATGAGTAAAGAACAATTGACATATAG
ACTACTCTCTATGGAAGTTGGAATCGAGAGTGGCAGGCTAAGAACAGGAAGATTACAGCAAGATGAATGGCCTTTACTTG
GAGAGGGTATCAATTCATTAGGTCAATTACCAATATTTATAGATGACAAACCTAACTTAAGTGTTTTAGAGATGAGATCT
CTATGCAGACGATTAATAGCTGAACAAAAAAAAGAATTAGGATTAATTGTGATTGATTACCTCCAGTTGATGGAAGGATC
AACCCCTGATAATAGAGTGCAGGAACTTTCACGAATCACAAGAGGTCTTAAAAGCATGGCCAGAGAATTAAAAGTGCCAG
TAGTAGCTTTATCTCAGCTAAGTAGAGGAGTAGAGTCTAGAACAAATAAAAGACCAATGTTAAGCGATCTAAGAGAATCA
GGATCTATTGAACAAGATGCAGATTTAGTATTAATGATTTATAGAGATGAATACTATAATCCAGATACTGAAGATAGAGG
AATTACAGAGATCATTGTCACTAAACATAGAAATGGACCCGTAGGAACTGTTAAATTATTATTTGAACCTCAATTTACGA
GATTTAGAAATTTAGCTAATTAA

Upstream 100 bases:

>100_bases
CCAGATGTAAATGCAGAAGTAAATATTGAAGTAACAAGTTAATCAATTTGTTGCATTATTTTGAAAAGTAGTCAGAGTAT
ATATCTAAGCAATTAAAGAT

Downstream 100 bases:

>100_bases
ATCGATCCGAATAATGCAAGATCATCACTCAACAAATGAATCTTTTGACATTGTCGTCATTGGAGGAGGACATGCGGGAT
GTGAAGCAGCTATAACATCA

Product: DnaB replicative helicase

Products: NA

Alternate protein names: Asp dnaB intein [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MVSVPFPNNGQNKNFKKDFNSENSGLVPPQNVQAEEAVLGGILLDPDAIGRIADLIKPEAFYINAHQEIYRTALMLHSQG
KPTDLTSMSAWLADNGSLEKIGGNSKLVELVENVSSTASIEQVANLINDKFMRRQLIRSGNEVVQLGFDQTQDTNEVLDK
AEQKIFEISQEKPSKGLTQAAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGFQRSDLIIVAGRPSMGKTSIVLNL
AKNVAQSQDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLQQDEWPLLGEGINSLGQLPIFIDDKPNLSVLEMRS
LCRRLIAEQKKELGLIVIDYLQLMEGSTPDNRVQELSRITRGLKSMARELKVPVVALSQLSRGVESRTNKRPMLSDLRES
GSIEQDADLVLMIYRDEYYNPDTEDRGITEIIVTKHRNGPVGTVKLLFEPQFTRFRNLAN

Sequences:

>Translated_460_residues
MVSVPFPNNGQNKNFKKDFNSENSGLVPPQNVQAEEAVLGGILLDPDAIGRIADLIKPEAFYINAHQEIYRTALMLHSQG
KPTDLTSMSAWLADNGSLEKIGGNSKLVELVENVSSTASIEQVANLINDKFMRRQLIRSGNEVVQLGFDQTQDTNEVLDK
AEQKIFEISQEKPSKGLTQAAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGFQRSDLIIVAGRPSMGKTSIVLNL
AKNVAQSQDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLQQDEWPLLGEGINSLGQLPIFIDDKPNLSVLEMRS
LCRRLIAEQKKELGLIVIDYLQLMEGSTPDNRVQELSRITRGLKSMARELKVPVVALSQLSRGVESRTNKRPMLSDLRES
GSIEQDADLVLMIYRDEYYNPDTEDRGITEIIVTKHRNGPVGTVKLLFEPQFTRFRNLAN
>Mature_460_residues
MVSVPFPNNGQNKNFKKDFNSENSGLVPPQNVQAEEAVLGGILLDPDAIGRIADLIKPEAFYINAHQEIYRTALMLHSQG
KPTDLTSMSAWLADNGSLEKIGGNSKLVELVENVSSTASIEQVANLINDKFMRRQLIRSGNEVVQLGFDQTQDTNEVLDK
AEQKIFEISQEKPSKGLTQAAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGFQRSDLIIVAGRPSMGKTSIVLNL
AKNVAQSQDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLQQDEWPLLGEGINSLGQLPIFIDDKPNLSVLEMRS
LCRRLIAEQKKELGLIVIDYLQLMEGSTPDNRVQELSRITRGLKSMARELKVPVVALSQLSRGVESRTNKRPMLSDLRES
GSIEQDADLVLMIYRDEYYNPDTEDRGITEIIVTKHRNGPVGTVKLLFEPQFTRFRNLAN

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=440, Percent_Identity=42.7272727272727, Blast_Score=350, Evalue=8e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51256; Mature: 51256

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVPFPNNGQNKNFKKDFNSENSGLVPPQNVQAEEAVLGGILLDPDAIGRIADLIKPEA
CEECCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHCCEEECHHHHHHHHHHHCCCE
FYINAHQEIYRTALMLHSQGKPTDLTSMSAWLADNGSLEKIGGNSKLVELVENVSSTASI
EEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHH
EQVANLINDKFMRRQLIRSGNEVVQLGFDQTQDTNEVLDKAEQKIFEISQEKPSKGLTQA
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
AEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGFQRSDLIIVAGRPSMGKTSIVLNL
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCCCCCCEEEEECCCCCCCHHHHHHH
AKNVAQSQDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLQQDEWPLLGEGINSL
HHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
GQLPIFIDDKPNLSVLEMRSLCRRLIAEQKKELGLIVIDYLQLMEGSTPDNRVQELSRIT
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHH
RGLKSMARELKVPVVALSQLSRGVESRTNKRPMLSDLRESGSIEQDADLVLMIYRDEYYN
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCC
PDTEDRGITEIIVTKHRNGPVGTVKLLFEPQFTRFRNLAN
CCCCCCCCEEEEEEECCCCCCEEEEEEECCCHHHHHCCCC
>Mature Secondary Structure
MVSVPFPNNGQNKNFKKDFNSENSGLVPPQNVQAEEAVLGGILLDPDAIGRIADLIKPEA
CEECCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHCCEEECHHHHHHHHHHHCCCE
FYINAHQEIYRTALMLHSQGKPTDLTSMSAWLADNGSLEKIGGNSKLVELVENVSSTASI
EEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHH
EQVANLINDKFMRRQLIRSGNEVVQLGFDQTQDTNEVLDKAEQKIFEISQEKPSKGLTQA
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
AEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGFQRSDLIIVAGRPSMGKTSIVLNL
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCCCCCCEEEEECCCCCCCHHHHHHH
AKNVAQSQDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLQQDEWPLLGEGINSL
HHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
GQLPIFIDDKPNLSVLEMRSLCRRLIAEQKKELGLIVIDYLQLMEGSTPDNRVQELSRIT
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHH
RGLKSMARELKVPVVALSQLSRGVESRTNKRPMLSDLRESGSIEQDADLVLMIYRDEYYN
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCC
PDTEDRGITEIIVTKHRNGPVGTVKLLFEPQFTRFRNLAN
CCCCCCCCEEEEEEECCCCCCEEEEEEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]