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The map label for this gene is galE [C]

Identifier: 78780142

GI number: 78780142

Start: 1647901

End: 1649094

Strand: Direct

Name: galE [C]

Synonym: PMT9312_1757

Alternate gene names: 78780142

Gene position: 1647901-1649094 (Clockwise)

Preceding gene: 78780141

Following gene: 78780143

Centisome position: 96.41

GC content: 35.01

Gene sequence:

>1194_bases
GTGAAAGTTATTGTTCTTGGTGGAGATGGTTTTTGCGGTTGGCCTTGTGCGGTGAATTTAGCAGAGCAAAATCATGATGT
AATAATTGTCGACAATTTAAGTCGTAGAAAAATAGATATTGATCTAGAAGTAGAATCTTTAACTCCGATCTCTTCGATAA
CAGAAAGACTTTCTGCATGGGAAGAGATTGGTGGTAAACCTATGAGATTTCTTAATATGGATATCTCTAAACAATATCAA
AAATTACTGAATTTGCTTATTGAAGAGAAACCAGATTCAGTTATCCATTTCGCAGAACAAAGAGCAGCGCCTTACTCGAT
GAAATCGAGTTTTACGAAAAGATATACAGTTGATAATAATGTTAATGGCACGCACAACCTTCTAGCTGCAATCGTAGAAT
CTAATTTAGATATTCATGTTGTTCATTTAGGAACAATGGGAGTCTACGGATATGGATCCCATAGAGGTGCAACAATTCCA
GAAGGTTATTTAAAGGTTGAAGTTCCACAACCAGATGGAAGCCGCTTTGAAGAAGAAATATTACACCCTGCAAGTCCAGG
TAGTGTTTACCATATGACAAAAACTTTAGATCAATTATTATTTCTTTACTACAACAAAAATGATCTTGTAAGAATAACTG
ATCTGCATCAAGGTATTGTTTGGGGAACAAATACAGAAGCAACTTTGAAAGATCCTAGATTGACAAACAGATTTGATTAT
GACGGAGATTATGGAACTGTTCTAAACAGATTTCTCATGCAAGCTGCTATTGGATATCCTTTAAGTGTTCATGGAACAGG
AGGGCAAACAAGAGCATTTATACATATAAAAGATTCGGTAAAATGTGTGCAACTTGCTCTTGAGAATCCTCCAAAATCTG
GAGAGAGAGTCAAAATCTTTAATCAAATGACTGAAAGTCATCAAGTTGGGGAGCTAGCTAAAAAAATTGCATCTCTCACA
GGGGCTGATATCAATTATTTACCAAATCCAAGGAACGAAGCCGTTGAAAATGATTTGATTGTTGATAATAAATGCTTTAT
AGAATTAGGTTTAAATCCAACCACTCTTGATAATGGCTTATTAGAAGAAGTTGTTGAAGTTGCTAAAAAATACTCCAAGA
GATGTGATTTAAAACGCATACCTTGTGTTTCATCCTGGACTAAAAAACAAGCTGAGGCTATAAAGACTAATTAA

Upstream 100 bases:

>100_bases
AAGAAGATTTGAAACTTTTTACAATGTTTTTTCGCAAATGGGAAGCACACTGTAGTAATTTAATAAATATATTATGAATC
TATTAATTCTGGAGTTCTGA

Downstream 100 bases:

>100_bases
AATTCCTGAAATAATTACCCGAAGAAAGTGAAAATTGCACTGTTTACTGAAACTTTTTTACCTAAAGTTGACGGCATAGT
CACAAGACTGACTAAAACGA

Product: sulfolipid (UDP-sulfoquinovose) biosynthesis protein

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Epimerase; UDP-Glucose 4-Epimerase; UDP Sulfoquinovose Synthase; Nucleoside-Diphosphate-Sugar Epimerase; Nucleotide Sugar Epimerase/Dehydratase; DTDP-Glucose 4-6-Dehydratase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAWEEIGGKPMRFLNMDISKQYQ
KLLNLLIEEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNNVNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIFNQMTESHQVGELAKKIASLT
GADINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGLLEEVVEVAKKYSKRCDLKRIPCVSSWTKKQAEAIKTN

Sequences:

>Translated_397_residues
MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAWEEIGGKPMRFLNMDISKQYQ
KLLNLLIEEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNNVNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIFNQMTESHQVGELAKKIASLT
GADINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGLLEEVVEVAKKYSKRCDLKRIPCVSSWTKKQAEAIKTN
>Mature_397_residues
MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAWEEIGGKPMRFLNMDISKQYQ
KLLNLLIEEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNNVNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIFNQMTESHQVGELAKKIASLT
GADINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGLLEEVVEVAKKYSKRCDLKRIPCVSSWTKKQAEAIKTN

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=150, Percent_Identity=32.6666666666667, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 44413; Mature: 44413

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAW
CEEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
EEIGGKPMRFLNMDISKQYQKLLNLLIEEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNN
HHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCEEEEECCC
VNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCCHHHHHHHHHHCCCCEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCEEEEEECCCCEEECCCCCCEECCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEEHHHCCCCCCCCEEEHH
NQMTESHQVGELAKKIASLTGADINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEECCEEEEEECCCCCCCCHHH
LEEVVEVAKKYSKRCDLKRIPCVSSWTKKQAEAIKTN
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAW
CEEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
EEIGGKPMRFLNMDISKQYQKLLNLLIEEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNN
HHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCEEEEECCC
VNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCCHHHHHHHHHHCCCCEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCEEEEEECCCCEEECCCCCCEECCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEEHHHCCCCCCCCEEEHH
NQMTESHQVGELAKKIASLTGADINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEECCEEEEEECCCCCCCCHHH
LEEVVEVAKKYSKRCDLKRIPCVSSWTKKQAEAIKTN
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA