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The map label for this gene is smpB

Identifier: 78780094

GI number: 78780094

Start: 1598418

End: 1598912

Strand: Direct

Name: smpB

Synonym: PMT9312_1709

Alternate gene names: 78780094

Gene position: 1598418-1598912 (Clockwise)

Preceding gene: 78780088

Following gene: 78780101

Centisome position: 93.52

GC content: 28.08

Gene sequence:

>495_bases
ATGGCAAAAAATTCAAACAAAGTTAGAAAAAATACTAATAAAGAAAATAATTTTAAACGTCTAGCTGAGAATAGATATGC
AAAATTTCAATATGCAATATCTGAAACAATCGAAGCTGGAATTGAACTTTTAGGAACTGAAGTAAAATCTATTAGAAACG
GAAAAGCAAATTTAAGAGATGGGTACTGTTCATTCAGAGATGGTGAGATTTTATTATTAAATGTTCACATTTCACCACAC
AAAAATGTGGGGTCTTTCTTTAATCATGATCCATTAAGAAATAGAAAGTTGCTACTACATAAAAAAGAAATAATTAAGCT
GAAATCCAATACTGAAAAAAAAGGAATGACTATTGTTCCCTTATCTATGTATTTAAAAGGGTCATGGATAAAACTAACTC
TTGGTGTCGGTAAAGGGAAAAAATTACATGACAAACGACAAGATGAAAAACAAAAAAGTATAAAAAAAGAAATCAACTCT
GTACTAAAAAGATAA

Upstream 100 bases:

>100_bases
ACGAAGAGAAAAGTCATTATCGCCTATATTGGAGGAAATTATTGCCATAATGAAAGGTTAATTGCAATTGTTCTAAGTAG
AAAGATTTTTTACAGCTAAA

Downstream 100 bases:

>100_bases
AAATATTATGCAGACTTCTTGAAACTATGAATCCAAACTTACTGAACTTGGAGGCGGGGAAGGATCTGGATCTGCAATTA
ACATATGCTGTAAGACAGTT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAKNSNKVRKNTNKENNFKRLAENRYAKFQYAISETIEAGIELLGTEVKSIRNGKANLRDGYCSFRDGEILLLNVHISPH
KNVGSFFNHDPLRNRKLLLHKKEIIKLKSNTEKKGMTIVPLSMYLKGSWIKLTLGVGKGKKLHDKRQDEKQKSIKKEINS
VLKR

Sequences:

>Translated_164_residues
MAKNSNKVRKNTNKENNFKRLAENRYAKFQYAISETIEAGIELLGTEVKSIRNGKANLRDGYCSFRDGEILLLNVHISPH
KNVGSFFNHDPLRNRKLLLHKKEIIKLKSNTEKKGMTIVPLSMYLKGSWIKLTLGVGKGKKLHDKRQDEKQKSIKKEINS
VLKR
>Mature_163_residues
AKNSNKVRKNTNKENNFKRLAENRYAKFQYAISETIEAGIELLGTEVKSIRNGKANLRDGYCSFRDGEILLLNVHISPHK
NVGSFFNHDPLRNRKLLLHKKEIIKLKSNTEKKGMTIVPLSMYLKGSWIKLTLGVGKGKKLHDKRQDEKQKSIKKEINSV
LKR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=143, Percent_Identity=42.6573426573427, Blast_Score=124, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18888; Mature: 18757

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNSNKVRKNTNKENNFKRLAENRYAKFQYAISETIEAGIELLGTEVKSIRNGKANLRD
CCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GYCSFRDGEILLLNVHISPHKNVGSFFNHDPLRNRKLLLHKKEIIKLKSNTEKKGMTIVP
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHEEEHHHHHEECCCCCCCCEEEEE
LSMYLKGSWIKLTLGVGKGKKLHDKRQDEKQKSIKKEINSVLKR
HHHEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKNSNKVRKNTNKENNFKRLAENRYAKFQYAISETIEAGIELLGTEVKSIRNGKANLRD
CCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GYCSFRDGEILLLNVHISPHKNVGSFFNHDPLRNRKLLLHKKEIIKLKSNTEKKGMTIVP
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHEEEHHHHHEECCCCCCCCEEEEE
LSMYLKGSWIKLTLGVGKGKKLHDKRQDEKQKSIKKEINSVLKR
HHHEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA