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The map label for this gene is trpE [H]

Identifier: 78780054

GI number: 78780054

Start: 1558045

End: 1559565

Strand: Reverse

Name: trpE [H]

Synonym: PMT9312_1669

Alternate gene names: 78780054

Gene position: 1559565-1558045 (Counterclockwise)

Preceding gene: 78780055

Following gene: 78780053

Centisome position: 91.25

GC content: 33.0

Gene sequence:

>1521_bases
ATGATCAGCTCAAATAAAGATAGTTTTTTAAAAGCTTACAAAGAAGGTAAAAATTTTATACCTATCACTCAAACTTGGCC
AGCAGATTTAGAGACTCCATTATCTACTTGGTTAAAATTATCCGAAAAAGATTCACATGGAGTTTTTCTTGAGTCTGTTG
AAGGAGGAGAGAGTTTGGGAAGGTGGAGTATCGTAGCTACTAAACCTCTTTGGGAAGCCGTTTGTTATGGAGAAGAAATA
GTTAAAACCTGGAATAATGGGAAAACGGAAATGTATAAGGGCGATCCCTTTAATATTTTAAGAACTTGGACAAAGGAATA
TAAATCATCTATGCTTGATGGCTTACCATCAATTGGGCAATTATATGGGTCTTGGGGTTATGAATTAATTAATCGAATAG
AACCAAGTGTTCCAATAAATAAAATACAAGAAAACAATATACCTTATGGTTCTTGGATGTTTTTTGATCAGTTAGTTGTT
TTTGATCAAATTAAAAGATGTATAACCGCAGTAGTTTATGCAGATATAACTTCTTCAAAAGAGTCTTCTGTTGAAGAAGT
TTATCTAAACTCAATTTCTAAAATTCAGAAAACTAGAGATTTAATGAAAGTCCCTCTAAAAGAATACGAGTTTTTAGATT
GGAATGAAAATGAGAATTTCAATTTAGATCTTAAAAGTAATTGGAAGAAAAAAGATTTTGAGGATGCAGTTCTTTCTGCA
AAAAAATACATAAGAAAAGGGGATATCTTCCAAATTGTTATAAGTCAGAGATTTCAAACTCAAGTCAATAATGATCCCTT
TAATTTATACAGAAGTTTGAGGATGGTTAATCCTTCCCCATATATGTCATTTTTTGATTTTGGCTCATGGTATCTGATAG
GTTCCAGTCCTGAAGTCATGGTTAAAGCTGAAAAAAATAAAAATAGTCAGATAGTTGCAAGCTTAAGACCAATAGCTGGG
ACAAGACCTAGGGGAATTGATAATAAACAAGATCTGGAATTTGAAAAAGATTTATTAAAAGATCCAAAAGAGATAGCTGA
GCATGTAATGCTAATAGATCTTGGAAGAAATGACCTTGGAAGAGTTTGTGAAATTGGTACTGTGGAGGTAAAAGATTTAA
TGGTTATTGAGAAATATTCACATGTTATGCATATAGTGAGTGAAGTTGAGGGAATCTTAAAAGAAAATACTGATGTATGG
GATTTGCTAAAGGCATGTTTTCCAGCCGGGACAGTAACTGGTGCGCCAAAAATAAGAGCTATGCAATTGATTAAACACTT
TGAAAAAGATGCTAGAGGACCTTATGCTGGTGTTTATGGATCTATTGATATTAATGGAGCATTAAATACGGCAATTACAA
TAAGAACTATGATAGTCAAACCCTCAGAAGATGGAAAATTTGATGTCTCAGTGCAGGCAGGAGCTGGAATAGTTGCTGAT
TCTTCTCCTGAAAATGAATATCAAGAGACAATAAATAAAGCTAAGGGGATACTGAAATCATTAGCCTGTTTGGATAATTA
A

Upstream 100 bases:

>100_bases
CAGCTGGGTTGAAATTTACAACTAAGAATACTTTTGATTAAATTACTCAAAGTTGATATAAAAGAAGAATATATTTATAA
TTTGGGTTGATACTATTAGT

Downstream 100 bases:

>100_bases
ATGAGTAAAAATAATCTTTATAAGGGTTTTGAAGTAGAACTTTTCACAGGTTCGTTTAATTCTCATATTGGTATTTCAGC
TGATATTGAAAAAGAATTTT

Product: anthranilate synthase, component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MISSNKDSFLKAYKEGKNFIPITQTWPADLETPLSTWLKLSEKDSHGVFLESVEGGESLGRWSIVATKPLWEAVCYGEEI
VKTWNNGKTEMYKGDPFNILRTWTKEYKSSMLDGLPSIGQLYGSWGYELINRIEPSVPINKIQENNIPYGSWMFFDQLVV
FDQIKRCITAVVYADITSSKESSVEEVYLNSISKIQKTRDLMKVPLKEYEFLDWNENENFNLDLKSNWKKKDFEDAVLSA
KKYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVMVKAEKNKNSQIVASLRPIAG
TRPRGIDNKQDLEFEKDLLKDPKEIAEHVMLIDLGRNDLGRVCEIGTVEVKDLMVIEKYSHVMHIVSEVEGILKENTDVW
DLLKACFPAGTVTGAPKIRAMQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSEDGKFDVSVQAGAGIVAD
SSPENEYQETINKAKGILKSLACLDN

Sequences:

>Translated_506_residues
MISSNKDSFLKAYKEGKNFIPITQTWPADLETPLSTWLKLSEKDSHGVFLESVEGGESLGRWSIVATKPLWEAVCYGEEI
VKTWNNGKTEMYKGDPFNILRTWTKEYKSSMLDGLPSIGQLYGSWGYELINRIEPSVPINKIQENNIPYGSWMFFDQLVV
FDQIKRCITAVVYADITSSKESSVEEVYLNSISKIQKTRDLMKVPLKEYEFLDWNENENFNLDLKSNWKKKDFEDAVLSA
KKYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVMVKAEKNKNSQIVASLRPIAG
TRPRGIDNKQDLEFEKDLLKDPKEIAEHVMLIDLGRNDLGRVCEIGTVEVKDLMVIEKYSHVMHIVSEVEGILKENTDVW
DLLKACFPAGTVTGAPKIRAMQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSEDGKFDVSVQAGAGIVAD
SSPENEYQETINKAKGILKSLACLDN
>Mature_506_residues
MISSNKDSFLKAYKEGKNFIPITQTWPADLETPLSTWLKLSEKDSHGVFLESVEGGESLGRWSIVATKPLWEAVCYGEEI
VKTWNNGKTEMYKGDPFNILRTWTKEYKSSMLDGLPSIGQLYGSWGYELINRIEPSVPINKIQENNIPYGSWMFFDQLVV
FDQIKRCITAVVYADITSSKESSVEEVYLNSISKIQKTRDLMKVPLKEYEFLDWNENENFNLDLKSNWKKKDFEDAVLSA
KKYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVMVKAEKNKNSQIVASLRPIAG
TRPRGIDNKQDLEFEKDLLKDPKEIAEHVMLIDLGRNDLGRVCEIGTVEVKDLMVIEKYSHVMHIVSEVEGILKENTDVW
DLLKACFPAGTVTGAPKIRAMQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSEDGKFDVSVQAGAGIVAD
SSPENEYQETINKAKGILKSLACLDN

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=419, Percent_Identity=35.5608591885441, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1788114, Length=459, Percent_Identity=29.4117647058824, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1786809, Length=278, Percent_Identity=27.3381294964029, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=490, Percent_Identity=35.1020408163265, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324361, Length=415, Percent_Identity=26.0240963855422, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57380; Mature: 57380

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSNKDSFLKAYKEGKNFIPITQTWPADLETPLSTWLKLSEKDSHGVFLESVEGGESLG
CCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCC
RWSIVATKPLWEAVCYGEEIVKTWNNGKTEMYKGDPFNILRTWTKEYKSSMLDGLPSIGQ
CEEEEECCHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHH
LYGSWGYELINRIEPSVPINKIQENNIPYGSWMFFDQLVVFDQIKRCITAVVYADITSSK
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ESSVEEVYLNSISKIQKTRDLMKVPLKEYEFLDWNENENFNLDLKSNWKKKDFEDAVLSA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEECCCCCCCEEEEECCCCCCCHHHHHHHHH
KKYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVM
HHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCCEE
VKAEKNKNSQIVASLRPIAGTRPRGIDNKQDLEFEKDLLKDPKEIAEHVMLIDLGRNDLG
EEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHEEEEECCCCCHH
RVCEIGTVEVKDLMVIEKYSHVMHIVSEVEGILKENTDVWDLLKACFPAGTVTGAPKIRA
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHH
MQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSEDGKFDVSVQAGAGIVAD
HHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEEECCCEEEEC
SSPENEYQETINKAKGILKSLACLDN
CCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISSNKDSFLKAYKEGKNFIPITQTWPADLETPLSTWLKLSEKDSHGVFLESVEGGESLG
CCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCC
RWSIVATKPLWEAVCYGEEIVKTWNNGKTEMYKGDPFNILRTWTKEYKSSMLDGLPSIGQ
CEEEEECCHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHH
LYGSWGYELINRIEPSVPINKIQENNIPYGSWMFFDQLVVFDQIKRCITAVVYADITSSK
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ESSVEEVYLNSISKIQKTRDLMKVPLKEYEFLDWNENENFNLDLKSNWKKKDFEDAVLSA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEECCCCCCCEEEEECCCCCCCHHHHHHHHH
KKYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVM
HHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCCEE
VKAEKNKNSQIVASLRPIAGTRPRGIDNKQDLEFEKDLLKDPKEIAEHVMLIDLGRNDLG
EEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHEEEEECCCCCHH
RVCEIGTVEVKDLMVIEKYSHVMHIVSEVEGILKENTDVWDLLKACFPAGTVTGAPKIRA
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHH
MQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSEDGKFDVSVQAGAGIVAD
HHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEEECCCEEEEC
SSPENEYQETINKAKGILKSLACLDN
CCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 3141423 [H]