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The map label for this gene is truA

Identifier: 78780010

GI number: 78780010

Start: 1525038

End: 1525844

Strand: Reverse

Name: truA

Synonym: PMT9312_1626

Alternate gene names: 78780010

Gene position: 1525844-1525038 (Counterclockwise)

Preceding gene: 78780011

Following gene: 78780009

Centisome position: 89.27

GC content: 31.23

Gene sequence:

>807_bases
TTGAAAAGGATAGCTTTATTAGTCCAATATGATGGATCTAATTACTCAGGTTGGCAAAGACAAAAAAATGCAACTACAGT
TCAGGAAACTCTAGAGAGAGCTCTTTTTAAGATTACCCATCAAGTCGTAAAGACTTTTGCCGCAGGTAGAACTGATGCGG
GTGTTCATGCATCAGGGCAAGTAGTACACTTCAACATTGATTGTGTTATTCCAGCAAATAGTTATTCTGATATCTTAAAT
GGTTTGTTACCCTCAGCAATTAGAATCTTAGAATCAGTTGAGGTTAAAGATAGTTGGCATTCATGCTATTCAGCAATGTA
TAGACATTATCGATACGTCATTAATAACAGTAAATTCCCTAATTTGTTCATAAATAATTGGTCATGGCATAGATACCAAA
AAATATTAGATGAAGTTTTAATGTCAAATGCATCAAAGCTAATGGAAGGAGAGCATGATTTTTTTGCTTTCCAGAAAAGT
GGAAGTCAAAGGAAAAACTCTATTACAAAAATAAAAAATGTAGAAATTAAAAGAGTAGAGGATTTAATTTTAGTTGATAT
TAAAGCTACTGGTTTTCTATATGGAATGGTCCGTTTAATTGTTGGTCAATTAGTGTTAGTTGGAGAGAAAAAAATATCGC
CAGAAATTTTTACAGATATATGGGTAAACCAAAAAAAAAATGATGTTAAAGAATCTGCTCCAGCTAAGGGTTTATGTTTT
GTAAATGCTGTTTATGAAGAAAATGTTTTTAAAAAGATCAATAACAATGATTTTTTCCCAGTGTTTTTAATTAAGGGTTT
TTCTTAA

Upstream 100 bases:

>100_bases
TAGTTAGAACAGTCTCTAGAAAAGGTGATAACGCTCAGATGGCTATAATTGAGCTTGTTTAAATTGGTTTATAAAGGTTC
CTTTTATTAGAAAAAAACTT

Downstream 100 bases:

>100_bases
GTAAAGTTGATCAAAGCGAATTATTTGTGTAAATCATCCAAATTTGTTGTTTAAAATTCCTTCAATAAGGTAGAATTTAG
AACTAACTTTAAATTTATTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKRIALLVQYDGSNYSGWQRQKNATTVQETLERALFKITHQVVKTFAAGRTDAGVHASGQVVHFNIDCVIPANSYSDILN
GLLPSAIRILESVEVKDSWHSCYSAMYRHYRYVINNSKFPNLFINNWSWHRYQKILDEVLMSNASKLMEGEHDFFAFQKS
GSQRKNSITKIKNVEIKRVEDLILVDIKATGFLYGMVRLIVGQLVLVGEKKISPEIFTDIWVNQKKNDVKESAPAKGLCF
VNAVYEENVFKKINNNDFFPVFLIKGFS

Sequences:

>Translated_268_residues
MKRIALLVQYDGSNYSGWQRQKNATTVQETLERALFKITHQVVKTFAAGRTDAGVHASGQVVHFNIDCVIPANSYSDILN
GLLPSAIRILESVEVKDSWHSCYSAMYRHYRYVINNSKFPNLFINNWSWHRYQKILDEVLMSNASKLMEGEHDFFAFQKS
GSQRKNSITKIKNVEIKRVEDLILVDIKATGFLYGMVRLIVGQLVLVGEKKISPEIFTDIWVNQKKNDVKESAPAKGLCF
VNAVYEENVFKKINNNDFFPVFLIKGFS
>Mature_268_residues
MKRIALLVQYDGSNYSGWQRQKNATTVQETLERALFKITHQVVKTFAAGRTDAGVHASGQVVHFNIDCVIPANSYSDILN
GLLPSAIRILESVEVKDSWHSCYSAMYRHYRYVINNSKFPNLFINNWSWHRYQKILDEVLMSNASKLMEGEHDFFAFQKS
GSQRKNSITKIKNVEIKRVEDLILVDIKATGFLYGMVRLIVGQLVLVGEKKISPEIFTDIWVNQKKNDVKESAPAKGLCF
VNAVYEENVFKKINNNDFFPVFLIKGFS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=267, Percent_Identity=27.3408239700375, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI199559785, Length=267, Percent_Identity=28.4644194756554, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788657, Length=261, Percent_Identity=33.7164750957854, Blast_Score=154, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321112, Length=247, Percent_Identity=31.1740890688259, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PROM9 (Q318K9)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_398122.1
- ProteinModelPortal:   Q318K9
- SMR:   Q318K9
- STRING:   Q318K9
- GeneID:   3766445
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1626
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   PMAR74546:PMT9312_1626-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30696; Mature: 30696

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIALLVQYDGSNYSGWQRQKNATTVQETLERALFKITHQVVKTFAAGRTDAGVHASGQ
CCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
VVHFNIDCVIPANSYSDILNGLLPSAIRILESVEVKDSWHSCYSAMYRHYRYVINNSKFP
EEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NLFINNWSWHRYQKILDEVLMSNASKLMEGEHDFFAFQKSGSQRKNSITKIKNVEIKRVE
EEEEECCCHHHHHHHHHHHHHHCHHHHHCCCCHHEEECCCCCHHHHHHHHHHCCHHHEEC
DLILVDIKATGFLYGMVRLIVGQLVLVGEKKISPEIFTDIWVNQKKNDVKESAPAKGLCF
EEEEEEEHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCCHHHHHCCCCCCEEE
VNAVYEENVFKKINNNDFFPVFLIKGFS
EEHHHHHHHHHHCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKRIALLVQYDGSNYSGWQRQKNATTVQETLERALFKITHQVVKTFAAGRTDAGVHASGQ
CCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
VVHFNIDCVIPANSYSDILNGLLPSAIRILESVEVKDSWHSCYSAMYRHYRYVINNSKFP
EEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NLFINNWSWHRYQKILDEVLMSNASKLMEGEHDFFAFQKSGSQRKNSITKIKNVEIKRVE
EEEEECCCHHHHHHHHHHHHHHCHHHHHCCCCHHEEECCCCCHHHHHHHHHHCCHHHEEC
DLILVDIKATGFLYGMVRLIVGQLVLVGEKKISPEIFTDIWVNQKKNDVKESAPAKGLCF
EEEEEEEHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCCHHHHHCCCCCCEEE
VNAVYEENVFKKINNNDFFPVFLIKGFS
EEHHHHHHHHHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA