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The map label for this gene is cbiH [H]

Identifier: 78780001

GI number: 78780001

Start: 1517268

End: 1519088

Strand: Direct

Name: cbiH [H]

Synonym: PMT9312_1617

Alternate gene names: 78780001

Gene position: 1517268-1519088 (Clockwise)

Preceding gene: 78779997

Following gene: 78780002

Centisome position: 88.77

GC content: 31.52

Gene sequence:

>1821_bases
TTGAAAGGAATCGCAATAGGTCTATCTAGTAATTCAAAAGCAATTTTAAAAAGACTTCAAAAAACTGAATTTGTTGAAGA
GATATATATAGCAAGCTCTTTAAAAAATGATGATATAGAAGATGATCTTGTAAAAATACATAAGCCTAAGGAAATTTTAC
TTAAAAAATGGCCAGAACTAGATTTAATAATTTTTATAGGATCAATTGGTGCATCGATACGTGTAATAAGTCCTTTTTTA
ACCTCTAAAGATAAAGATCCAGGCGTGATAGCAATAGATAACAAATGTTCCAAGATAGTGCCTTTAATTGGTTTACATCA
GTCAAATACCCAAAACATCGCATTTCAAATTTCTAATTTATTTGGTGGCCAAATAATAGAGACTAATAATTCAAATGATC
AAAGCTTTTTAAATCTTGATTCATTTGGGAATCAATGGGGTTGGAAAAGATCTGGCAAAATACAGAACTGGTCAAAACTA
GTAATAAATCAAGCTAAGAACAAAGAAATATTTTGCAAACAATTATCTGGTAACAGTTTATGGAAAACTTCAGAATCAGG
AGAGTTGATTAATAAACTTCATCAAAAGGAAACTGAAAAACCAGATTCAACATTTCATGTGAGTATATTCGAAACGCATG
TTACAACTTGGCATCCGCGCGTATTATGGATTGGCATTGGTTGTGAGCGAAATACTAGCAAAGAACTAATAGCAAATTCT
TTAAATAAAATTTTGGAGTTAGAAAATTTATCTCAGCATTCAATTGCGGGATTTGCAACTATTGATATTAAAAAAGATGA
GCAAGGGATTTTAGAACTCGCTGAAGAAAATAACTTGCCTATCAAGTTTTTTACTAAAGAAGATCTTTCAAAAATAATTG
TTCCAAATCCATCAAGTGTAGTTCAAAAGGAAATTGGCACATCTTCTGTAGCAGAAGCTTCATGCTTACTCGCAGCAGGA
GAAGAATCAAATTTATTAAAAGAAAAAATAATTTTTAAAGATAAAGATCTTCCAAAAAGTAAATCTGGCGCAGTTACTAT
TGCTATAGCTGAATCAAAAAATCAATATTATCCAAAAAATGGCGAAATACATATTATTGGAAGTGGGCCTGGCGATCTTT
CCTTTTTAACCAATAACGCAAGAAAAGCACTCTCAAGATGTAGTGTTTGGATTGGATACAAAATGTATTTAGATTTAATT
AAACCATTGAAAAGGAGTGACCAGGTTTCAATTGAAAGTAAACTTACTGAGGAAAAAGAAAGATGCAGCACAGCAATTAA
ACTAGCCGAGGAAGGAATAAAAGTGGCTTTAATTTCGTCAGGGGAATCTGGATTTTATGGCATGGCTGGTTTACTTTTAG
AATTACTTCTAAAAATCAAAAAAGAATATAGACCTTACTTTGAAGTACACCCTGGGATCAGTAGTGTTCAATTAGCCGCT
GCGCTTAGTGGAGCTCCACTAATGAATGACTTTTGTTCAATAAGCTTAAGTGATAAGTTAACGCCCTGGTCTTTAATAGA
AAAAAGAATTGAGGGAGCTCTTCTAGGTGACTTCGTAATAGCTTTATTTAATCCTCAATCAATTGAAAGAAATTGGCAGC
TAAAAAGTGCAATTGATCTATGTATAAAATTTAGGCAAAGTGAAACTCCCATTTTAATAGCTAGACAAGTAGGAAGAGAA
AATCAATCTAGAAAATTTTTTACTTTAAACACCATTCCCTTTGAAGAAATCGATATGTTATCAATAATTATTATTGGTAA
TTCTCAAACCACTTTAGTAGATGAAATATTTCTTACTCCAAGAGGATATTTACAAAAATAA

Upstream 100 bases:

>100_bases
TTTTGTAAGGATTTCTTTATATTTGTTTTATATTTCACACGATTTTTTTGAAATGCATTGAAAGTATATAATTTCCTTAT
AACAAATAAAATTTTTTGAT

Downstream 100 bases:

>100_bases
ATTTTGATTCATAAATTAATAATAGAACGTTTTTCTTTGCTTTTTTTTAATAAGAATACTAATCTTTTATAAATAAGGAT
TAAGAAAATTAATTGAAAAA

Product: precorrin-3 methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MKGIAIGLSSNSKAILKRLQKTEFVEEIYIASSLKNDDIEDDLVKIHKPKEILLKKWPELDLIIFIGSIGASIRVISPFL
TSKDKDPGVIAIDNKCSKIVPLIGLHQSNTQNIAFQISNLFGGQIIETNNSNDQSFLNLDSFGNQWGWKRSGKIQNWSKL
VINQAKNKEIFCKQLSGNSLWKTSESGELINKLHQKETEKPDSTFHVSIFETHVTTWHPRVLWIGIGCERNTSKELIANS
LNKILELENLSQHSIAGFATIDIKKDEQGILELAEENNLPIKFFTKEDLSKIIVPNPSSVVQKEIGTSSVAEASCLLAAG
EESNLLKEKIIFKDKDLPKSKSGAVTIAIAESKNQYYPKNGEIHIIGSGPGDLSFLTNNARKALSRCSVWIGYKMYLDLI
KPLKRSDQVSIESKLTEEKERCSTAIKLAEEGIKVALISSGESGFYGMAGLLLELLLKIKKEYRPYFEVHPGISSVQLAA
ALSGAPLMNDFCSISLSDKLTPWSLIEKRIEGALLGDFVIALFNPQSIERNWQLKSAIDLCIKFRQSETPILIARQVGRE
NQSRKFFTLNTIPFEEIDMLSIIIIGNSQTTLVDEIFLTPRGYLQK

Sequences:

>Translated_606_residues
MKGIAIGLSSNSKAILKRLQKTEFVEEIYIASSLKNDDIEDDLVKIHKPKEILLKKWPELDLIIFIGSIGASIRVISPFL
TSKDKDPGVIAIDNKCSKIVPLIGLHQSNTQNIAFQISNLFGGQIIETNNSNDQSFLNLDSFGNQWGWKRSGKIQNWSKL
VINQAKNKEIFCKQLSGNSLWKTSESGELINKLHQKETEKPDSTFHVSIFETHVTTWHPRVLWIGIGCERNTSKELIANS
LNKILELENLSQHSIAGFATIDIKKDEQGILELAEENNLPIKFFTKEDLSKIIVPNPSSVVQKEIGTSSVAEASCLLAAG
EESNLLKEKIIFKDKDLPKSKSGAVTIAIAESKNQYYPKNGEIHIIGSGPGDLSFLTNNARKALSRCSVWIGYKMYLDLI
KPLKRSDQVSIESKLTEEKERCSTAIKLAEEGIKVALISSGESGFYGMAGLLLELLLKIKKEYRPYFEVHPGISSVQLAA
ALSGAPLMNDFCSISLSDKLTPWSLIEKRIEGALLGDFVIALFNPQSIERNWQLKSAIDLCIKFRQSETPILIARQVGRE
NQSRKFFTLNTIPFEEIDMLSIIIIGNSQTTLVDEIFLTPRGYLQK
>Mature_606_residues
MKGIAIGLSSNSKAILKRLQKTEFVEEIYIASSLKNDDIEDDLVKIHKPKEILLKKWPELDLIIFIGSIGASIRVISPFL
TSKDKDPGVIAIDNKCSKIVPLIGLHQSNTQNIAFQISNLFGGQIIETNNSNDQSFLNLDSFGNQWGWKRSGKIQNWSKL
VINQAKNKEIFCKQLSGNSLWKTSESGELINKLHQKETEKPDSTFHVSIFETHVTTWHPRVLWIGIGCERNTSKELIANS
LNKILELENLSQHSIAGFATIDIKKDEQGILELAEENNLPIKFFTKEDLSKIIVPNPSSVVQKEIGTSSVAEASCLLAAG
EESNLLKEKIIFKDKDLPKSKSGAVTIAIAESKNQYYPKNGEIHIIGSGPGDLSFLTNNARKALSRCSVWIGYKMYLDLI
KPLKRSDQVSIESKLTEEKERCSTAIKLAEEGIKVALISSGESGFYGMAGLLLELLLKIKKEYRPYFEVHPGISSVQLAA
ALSGAPLMNDFCSISLSDKLTPWSLIEKRIEGALLGDFVIALFNPQSIERNWQLKSAIDLCIKFRQSETPILIARQVGRE
NQSRKFFTLNTIPFEEIDMLSIIIIGNSQTTLVDEIFLTPRGYLQK

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 67651; Mature: 67651

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIAIGLSSNSKAILKRLQKTEFVEEIYIASSLKNDDIEDDLVKIHKPKEILLKKWPEL
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHCCCCC
DLIIFIGSIGASIRVISPFLTSKDKDPGVIAIDNKCSKIVPLIGLHQSNTQNIAFQISNL
EEEEEEECCCCEEEEEEHHHCCCCCCCCEEEECCCCCHHCCEEEECCCCCCEEEEEEECC
FGGQIIETNNSNDQSFLNLDSFGNQWGWKRSGKIQNWSKLVINQAKNKEIFCKQLSGNSL
CCCEEEECCCCCCCEEEEHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCC
WKTSESGELINKLHQKETEKPDSTFHVSIFETHVTTWHPRVLWIGIGCERNTSKELIANS
CCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEECEECCEEEEEEECCCCCCHHHHHHHH
LNKILELENLSQHSIAGFATIDIKKDEQGILELAEENNLPIKFFTKEDLSKIIVPNPSSV
HHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEHHCCCEEECCCCHHH
VQKEIGTSSVAEASCLLAAGEESNLLKEKIIFKDKDLPKSKSGAVTIAIAESKNQYYPKN
HHHHCCCCHHHHHHEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCC
GEIHIIGSGPGDLSFLTNNARKALSRCSVWIGYKMYLDLIKPLKRSDQVSIESKLTEEKE
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHH
RCSTAIKLAEEGIKVALISSGESGFYGMAGLLLELLLKIKKEYRPYFEVHPGISSVQLAA
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHH
ALSGAPLMNDFCSISLSDKLTPWSLIEKRIEGALLGDFVIALFNPQSIERNWQLKSAIDL
HHCCCCHHHHHHCEEECCCCCHHHHHHHHHCCHHHHHHHEEEECCHHHCCCCCHHHHHHH
CIKFRQSETPILIARQVGRENQSRKFFTLNTIPFEEIDMLSIIIIGNSQTTLVDEIFLTP
HHHHCCCCCCEEEEEHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEHEEECC
RGYLQK
CHHCCC
>Mature Secondary Structure
MKGIAIGLSSNSKAILKRLQKTEFVEEIYIASSLKNDDIEDDLVKIHKPKEILLKKWPEL
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHCCCCC
DLIIFIGSIGASIRVISPFLTSKDKDPGVIAIDNKCSKIVPLIGLHQSNTQNIAFQISNL
EEEEEEECCCCEEEEEEHHHCCCCCCCCEEEECCCCCHHCCEEEECCCCCCEEEEEEECC
FGGQIIETNNSNDQSFLNLDSFGNQWGWKRSGKIQNWSKLVINQAKNKEIFCKQLSGNSL
CCCEEEECCCCCCCEEEEHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCC
WKTSESGELINKLHQKETEKPDSTFHVSIFETHVTTWHPRVLWIGIGCERNTSKELIANS
CCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEECEECCEEEEEEECCCCCCHHHHHHHH
LNKILELENLSQHSIAGFATIDIKKDEQGILELAEENNLPIKFFTKEDLSKIIVPNPSSV
HHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEHHCCCEEECCCCHHH
VQKEIGTSSVAEASCLLAAGEESNLLKEKIIFKDKDLPKSKSGAVTIAIAESKNQYYPKN
HHHHCCCCHHHHHHEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCC
GEIHIIGSGPGDLSFLTNNARKALSRCSVWIGYKMYLDLIKPLKRSDQVSIESKLTEEKE
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHH
RCSTAIKLAEEGIKVALISSGESGFYGMAGLLLELLLKIKKEYRPYFEVHPGISSVQLAA
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHH
ALSGAPLMNDFCSISLSDKLTPWSLIEKRIEGALLGDFVIALFNPQSIERNWQLKSAIDL
HHCCCCHHHHHHCEEECCCCCHHHHHHHHHCCHHHHHHHEEEECCHHHCCCCCHHHHHHH
CIKFRQSETPILIARQVGRENQSRKFFTLNTIPFEEIDMLSIIIIGNSQTTLVDEIFLTP
HHHHCCCCCCEEEEEHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEHEEECC
RGYLQK
CHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]