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The map label for this gene is tuf [H]

Identifier: 78779984

GI number: 78779984

Start: 1496941

End: 1498140

Strand: Reverse

Name: tuf [H]

Synonym: PMT9312_1600

Alternate gene names: 78779984

Gene position: 1498140-1496941 (Counterclockwise)

Preceding gene: 78779985

Following gene: 78779983

Centisome position: 87.65

GC content: 39.67

Gene sequence:

>1200_bases
ATGGCTCGCGAGAAGTTCGAAAGGAACAAACCACATGTCAACATAGGTACTATTGGCCATGTTGATCATGGAAAAACAAC
ACTTACTGCTGCTATTACAAACGTATTAGCAAAAAAAGGTCAAGCTCAAGCTCAAGACTATGGAGACATTGATGGTGCTC
CTGAAGAAAGAGAGCGCGGCATTACTATTAATACAGCTCACGTCGAATATGAAACTGAAGGCAGGCATTATGCTCATGTT
GATTGCCCTGGACATGCTGATTATGTAAAAAACATGATTACAGGAGCCGCCCAAATGGACGGAGCTATTCTAGTTTGTGC
AGCCACCGATGGCCCTATGGCGCAAACAAAAGAGCATATTCTTCTAGCTAAACAGGTTGGAGTACCTGCTCTTGTAGTAG
CTCTTAACAAGTGCGATATGGTCGATGATGAAGAAATTATTGAACTTGTTGAAATGGAAATCAGAGAACTATTAGATAGT
TATGATTTCCCTGGAGATGATATTCCTATAGTTCAAGTTTCAGGTTTAAAAGCTCTTGAAGGTGATTCTACTTGGGAATC
AAAAATAGAAGAATTAATGACAGCAGTTGATGCAAGCATTCCTGAACCAGAAAGAGAAGTTGATAAACCATTCTTGATGG
CAGTTGAAGACGTTTTCTCAATTACTGGTAGAGGAACTGTAGCTACTGGAAGAATTGAAAGAGGAAAAGTTAAGGTTGGA
GAAGAAGTTGAAATAGTTGGAATAAGAGATACAAGATTGACAACAGTTACTGGAGTTGAAATGTTCCGTAAACTTCTTGA
TGAAGGTATGGCTGGTGATAATGTTGGTTTACTTTTACGTGGTGTTCAGAAAGAGGATATTGAGAGAGGAATGGTACTCG
TTAAGAAAGGATCCATTACCCCTCATACTCAGTTTGAAGGAGAAGTTTACGTTCTAAAGAAAGAAGAGGGCGGCAGACAT
ACACCTTTCTTCGCAGGTTATAGGCCTCAGTTTTATATCCGAACAACTGATGTGACAGGTCAAATCACAGCCTTTACTTC
TGATGACGGTTCTAATGTTGAAATGGTTATGCCAGGTGACAGAATCAAGATGACTGGAGAATTAATTTGTCCAGTAGCTA
TTGAACAAGGTATGCGATTTGCAATTCGTGAAGGTGGACGTACTATTGGTGCTGGAGTTGTTTCTAAAATTCTCAAATAA

Upstream 100 bases:

>100_bases
AGTCCCTCGTAATGTTGCTGAAGCTATCATTACCAAGAATCAGGGCAACTCCTGATCTCTAAACTAAAACACTCTTAAAC
CCTCGATTCTTTAATTCAAA

Downstream 100 bases:

>100_bases
GACGTTTGAATTTTAAGAATTTAACCTCAATCACTTAAAATAAGTCAATGGACTAGGGCCATTGTTAATCAATGGCCTAC
CACTAGAAATTAATTTGAAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQAQDYGDIDGAPEERERGITINTAHVEYETEGRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLDS
YDFPGDDIPIVQVSGLKALEGDSTWESKIEELMTAVDASIPEPEREVDKPFLMAVEDVFSITGRGTVATGRIERGKVKVG
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGVQKEDIERGMVLVKKGSITPHTQFEGEVYVLKKEEGGRH
TPFFAGYRPQFYIRTTDVTGQITAFTSDDGSNVEMVMPGDRIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKILK

Sequences:

>Translated_399_residues
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQAQDYGDIDGAPEERERGITINTAHVEYETEGRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLDS
YDFPGDDIPIVQVSGLKALEGDSTWESKIEELMTAVDASIPEPEREVDKPFLMAVEDVFSITGRGTVATGRIERGKVKVG
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGVQKEDIERGMVLVKKGSITPHTQFEGEVYVLKKEEGGRH
TPFFAGYRPQFYIRTTDVTGQITAFTSDDGSNVEMVMPGDRIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKILK
>Mature_398_residues
AREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQAQDYGDIDGAPEERERGITINTAHVEYETEGRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLDSY
DFPGDDIPIVQVSGLKALEGDSTWESKIEELMTAVDASIPEPEREVDKPFLMAVEDVFSITGRGTVATGRIERGKVKVGE
EVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGVQKEDIERGMVLVKKGSITPHTQFEGEVYVLKKEEGGRHT
PFFAGYRPQFYIRTTDVTGQITAFTSDDGSNVEMVMPGDRIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKILK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=399, Percent_Identity=54.1353383458647, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI4503475, Length=457, Percent_Identity=30.4157549234136, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=30.0683371298405, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=30.0683371298405, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI4503471, Length=453, Percent_Identity=30.0220750551876, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI54607086, Length=323, Percent_Identity=29.1021671826625, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI46094014, Length=435, Percent_Identity=28.2758620689655, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI194018522, Length=436, Percent_Identity=27.0642201834862, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI194097354, Length=436, Percent_Identity=27.0642201834862, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI194018520, Length=436, Percent_Identity=27.0642201834862, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=28.0898876404494, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI157426893, Length=274, Percent_Identity=25.5474452554745, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI53729339, Length=234, Percent_Identity=32.9059829059829, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI53729337, Length=234, Percent_Identity=32.9059829059829, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790412, Length=398, Percent_Identity=72.6130653266332, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1789737, Length=398, Percent_Identity=72.6130653266332, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI2367247, Length=334, Percent_Identity=32.0359281437126, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789108, Length=334, Percent_Identity=31.437125748503, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=31.9502074688797, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788922, Length=149, Percent_Identity=33.5570469798658, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=406, Percent_Identity=49.7536945812808, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=45.8974358974359, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=31.1804008908686, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=31.1804008908686, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=28.8636363636364, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=28.8990825688073, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=28.5714285714286, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=451, Percent_Identity=27.9379157427938, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=271, Percent_Identity=31.3653136531365, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=40.1360544217687, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=28.0172413793103, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=269, Percent_Identity=27.1375464684015, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17565438, Length=437, Percent_Identity=22.883295194508, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994658, Length=177, Percent_Identity=35.5932203389831, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=61.9647355163728, Blast_Score=496, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=31.3199105145414, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=31.3199105145414, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=452, Percent_Identity=25.4424778761062, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6320377, Length=439, Percent_Identity=27.3348519362187, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=273, Percent_Identity=27.4725274725275, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=30.2904564315353, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=32.5925925925926, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=57.4683544303797, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=57.4683544303797, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI19921738, Length=393, Percent_Identity=45.5470737913486, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=30, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=30, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=30, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=30, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24652838, Length=444, Percent_Identity=29.7297297297297, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17137572, Length=444, Percent_Identity=29.7297297297297, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.6930693069307, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45550900, Length=452, Percent_Identity=27.4336283185841, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17137380, Length=452, Percent_Identity=27.6548672566372, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647048, Length=267, Percent_Identity=28.4644194756554, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647046, Length=267, Percent_Identity=28.4644194756554, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI78706572, Length=260, Percent_Identity=26.1538461538462, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=34.7560975609756, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582462, Length=156, Percent_Identity=32.6923076923077, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43625; Mature: 43494

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQAQDYGDIDGAPEERERG
CCCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCC
ITINTAHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHE
LLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLDSYDFPGDDIPIVQVSGLKALE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEC
GDSTWESKIEELMTAVDASIPEPEREVDKPFLMAVEDVFSITGRGTVATGRIERGKVKVG
CCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEECCEECCCEECC
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGVQKEDIERGMVLVKKGSIT
CEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEEECCCCC
PHTQFEGEVYVLKKEEGGRHTPFFAGYRPQFYIRTTDVTGQITAFTSDDGSNVEMVMPGD
CCCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEEECCCEEEEEEECCCCCCEEEEECCC
RIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKILK
CEEECCCEECHHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQAQDYGDIDGAPEERERG
CCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCC
ITINTAHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHE
LLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLDSYDFPGDDIPIVQVSGLKALE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEC
GDSTWESKIEELMTAVDASIPEPEREVDKPFLMAVEDVFSITGRGTVATGRIERGKVKVG
CCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEECCEECCCEECC
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGVQKEDIERGMVLVKKGSIT
CEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEEECCCCC
PHTQFEGEVYVLKKEEGGRHTPFFAGYRPQFYIRTTDVTGQITAFTSDDGSNVEMVMPGD
CCCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEEECCCEEEEEEECCCCCCEEEEECCC
RIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKILK
CEEECCCEECHHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA