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The map label for this gene is murD

Identifier: 78779845

GI number: 78779845

Start: 1371211

End: 1372617

Strand: Direct

Name: murD

Synonym: PMT9312_1461

Alternate gene names: 78779845

Gene position: 1371211-1372617 (Clockwise)

Preceding gene: 78779843

Following gene: 78779846

Centisome position: 80.23

GC content: 27.65

Gene sequence:

>1407_bases
ATGATAGATAATAATCATTTAAGAAAAAGAAAAAATATTAATCTTGTAATTGGATTAGGTAAATCTGGAGTTTGGGCTGC
CAAGTATTTGAGAAGTATCGATAAGAGAGTAATTATTTGGGAAAGTAAAGATGGGAAAGAATTCTTAGAAATAAAAACAG
AATTGGAGGAACTTAATATATTAGTTTCTCTGAATAAAGAATTTGTATTTAAAGAAATTCATCCCTTTATAAAAGAGATT
GAATCTGTTGTTGTAAGTCCATCAATACCTTATGACCATGAAACTATTATTAAATTAAAAAAAAAGGGAATAAAAGTAAT
TGGAGAAATTAATGTTGCATGGGAAATTTTAAAAGAGACAAATTGGATAGGTATTACTGGCACTAATGGTAAGACTACTG
TTACTCATTTACTAAGTCATATACTCTGCGAGAATAAATTATATGCTCCTTTTGCTGGAAATATTGGTACACCTTTATGT
AAATATGCTTACTCCAAAAAATACGAAAAAATTGACTGGGTTGTAGCTGAATTAAGCAGTTATCAAATAGAAATATCTCC
AGAAGTAAAACCTAATATAGGAATCTGGACAACCTTCACAGAAGATCATCTTGAAAGACATAAAACACTTGAAAACTATT
TCAACATAAAAAAAAGCTTGCTAGAAAAATCAGATTTCAGAATTTATAATTATGACGATAAAAATCTAAGAAATCACTAT
AGTTCACTATCAAAAGGGGTTTGGATAACAACTAGTCTAGATAAATCAAATTTCATTCAATGCGATTATTGGATAGATGA
TCAAGCATTCATTATTGAGAGAGGAAAGAGACTATTCAAACTTGAACATTTTTCTTTAAAAGGAATTCATAATCTTCAAA
ATCTTCTATTAGTAATAGCAGCAGCAAGAAAAGTTGGATTGTCAGGAAAGAAGATTAAAGATTCTTTATCTAATTACAAA
CAATTACCGCATAGGATGGAAACAATTTACAAAAATAATGATCTGGAAATAATTAATGATAGTAAAGCTACGAATTTTGA
TTCATCTATTGCAGGAATAAATTCAATTGAAGGTCAAATAATAATAATTTCTGGAGGCAGATTAAAAGGCAATAAATACA
GTGAGTGGGTACAAGTTTTAAAGCAAAAAGTTAAATGTATTTTTCTTTTTGGAGAAAGCTCAAAAGTCCTAAAGATGGCC
CTTATTAATGAAGGATTTAAAAAAGATATTTTTGAATTTTCAGAACTCAAAGAGCTTTTAAATTTTGTTTTTCATTATTT
ACAAAATAATAAAGTTGGAACATTATTATTCTCACCATCATGCTCAAGTTTTGATCAATTTAAAAATTATGAAGAGCGAG
GAGATCATTTCAAGAAACTAATAAGTGAAAAATTAAAGGTTAATTAA

Upstream 100 bases:

>100_bases
TAATTAACTTTTATTTGGCGAATTCTATAAATACTAAAGAGTGATCTCATGATTGAATTAACCCTCAAACACATTTAATA
TAAATTCAATTATAAATTTA

Downstream 100 bases:

>100_bases
TTCAGTTTTTATTTAAAGATCATTAAGTCATTTTCAGGTCGGTCATCACAACCGTCTCACCCCTAGCGAATATTCACTTA
ATTAGTTAGATTTTGACTAT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MIDNNHLRKRKNINLVIGLGKSGVWAAKYLRSIDKRVIIWESKDGKEFLEIKTELEELNILVSLNKEFVFKEIHPFIKEI
ESVVVSPSIPYDHETIIKLKKKGIKVIGEINVAWEILKETNWIGITGTNGKTTVTHLLSHILCENKLYAPFAGNIGTPLC
KYAYSKKYEKIDWVVAELSSYQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNHY
SSLSKGVWITTSLDKSNFIQCDYWIDDQAFIIERGKRLFKLEHFSLKGIHNLQNLLLVIAAARKVGLSGKKIKDSLSNYK
QLPHRMETIYKNNDLEIINDSKATNFDSSIAGINSIEGQIIIISGGRLKGNKYSEWVQVLKQKVKCIFLFGESSKVLKMA
LINEGFKKDIFEFSELKELLNFVFHYLQNNKVGTLLFSPSCSSFDQFKNYEERGDHFKKLISEKLKVN

Sequences:

>Translated_468_residues
MIDNNHLRKRKNINLVIGLGKSGVWAAKYLRSIDKRVIIWESKDGKEFLEIKTELEELNILVSLNKEFVFKEIHPFIKEI
ESVVVSPSIPYDHETIIKLKKKGIKVIGEINVAWEILKETNWIGITGTNGKTTVTHLLSHILCENKLYAPFAGNIGTPLC
KYAYSKKYEKIDWVVAELSSYQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNHY
SSLSKGVWITTSLDKSNFIQCDYWIDDQAFIIERGKRLFKLEHFSLKGIHNLQNLLLVIAAARKVGLSGKKIKDSLSNYK
QLPHRMETIYKNNDLEIINDSKATNFDSSIAGINSIEGQIIIISGGRLKGNKYSEWVQVLKQKVKCIFLFGESSKVLKMA
LINEGFKKDIFEFSELKELLNFVFHYLQNNKVGTLLFSPSCSSFDQFKNYEERGDHFKKLISEKLKVN
>Mature_468_residues
MIDNNHLRKRKNINLVIGLGKSGVWAAKYLRSIDKRVIIWESKDGKEFLEIKTELEELNILVSLNKEFVFKEIHPFIKEI
ESVVVSPSIPYDHETIIKLKKKGIKVIGEINVAWEILKETNWIGITGTNGKTTVTHLLSHILCENKLYAPFAGNIGTPLC
KYAYSKKYEKIDWVVAELSSYQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNHY
SSLSKGVWITTSLDKSNFIQCDYWIDDQAFIIERGKRLFKLEHFSLKGIHNLQNLLLVIAAARKVGLSGKKIKDSLSNYK
QLPHRMETIYKNNDLEIINDSKATNFDSSIAGINSIEGQIIIISGGRLKGNKYSEWVQVLKQKVKCIFLFGESSKVLKMA
LINEGFKKDIFEFSELKELLNFVFHYLQNNKVGTLLFSPSCSSFDQFKNYEERGDHFKKLISEKLKVN

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=462, Percent_Identity=26.4069264069264, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_PROM9 (Q319C4)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397957.1
- ProteinModelPortal:   Q319C4
- SMR:   Q319C4
- STRING:   Q319C4
- GeneID:   3766278
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1461
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- ProtClustDB:   PRK02705
- BioCyc:   PMAR74546:PMT9312_1461-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 54131; Mature: 54131

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNNHLRKRKNINLVIGLGKSGVWAAKYLRSIDKRVIIWESKDGKEFLEIKTELEELNI
CCCCCCCHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCEE
LVSLNKEFVFKEIHPFIKEIESVVVSPSIPYDHETIIKLKKKGIKVIGEINVAWEILKET
EEECCHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHCC
NWIGITGTNGKTTVTHLLSHILCENKLYAPFAGNIGTPLCKYAYSKKYEKIDWVVAELSS
CEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHEECCC
YQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNHY
EEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
SSLSKGVWITTSLDKSNFIQCDYWIDDQAFIIERGKRLFKLEHFSLKGIHNLQNLLLVIA
HHHHCCEEEEEECCCCCCEEEEEEECCCHHHHHCCCHHEEEHHHCCCCHHHHHHHHHHHH
AARKVGLSGKKIKDSLSNYKQLPHRMETIYKNNDLEIINDSKATNFDSSIAGINSIEGQI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCCCEE
IIISGGRLKGNKYSEWVQVLKQKVKCIFLFGESSKVLKMALINEGFKKDIFEFSELKELL
EEEECCEECCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
NFVFHYLQNNKVGTLLFSPSCSSFDQFKNYEERGDHFKKLISEKLKVN
HHHHHHHCCCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDNNHLRKRKNINLVIGLGKSGVWAAKYLRSIDKRVIIWESKDGKEFLEIKTELEELNI
CCCCCCCHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCEE
LVSLNKEFVFKEIHPFIKEIESVVVSPSIPYDHETIIKLKKKGIKVIGEINVAWEILKET
EEECCHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHCC
NWIGITGTNGKTTVTHLLSHILCENKLYAPFAGNIGTPLCKYAYSKKYEKIDWVVAELSS
CEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHEECCC
YQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNHY
EEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
SSLSKGVWITTSLDKSNFIQCDYWIDDQAFIIERGKRLFKLEHFSLKGIHNLQNLLLVIA
HHHHCCEEEEEECCCCCCEEEEEEECCCHHHHHCCCHHEEEHHHCCCCHHHHHHHHHHHH
AARKVGLSGKKIKDSLSNYKQLPHRMETIYKNNDLEIINDSKATNFDSSIAGINSIEGQI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCCCEE
IIISGGRLKGNKYSEWVQVLKQKVKCIFLFGESSKVLKMALINEGFKKDIFEFSELKELL
EEEECCEECCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
NFVFHYLQNNKVGTLLFSPSCSSFDQFKNYEERGDHFKKLISEKLKVN
HHHHHHHCCCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA