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The map label for this gene is truB

Identifier: 78779829

GI number: 78779829

Start: 1357704

End: 1358627

Strand: Direct

Name: truB

Synonym: PMT9312_1445

Alternate gene names: 78779829

Gene position: 1357704-1358627 (Clockwise)

Preceding gene: 78779827

Following gene: 78779831

Centisome position: 79.43

GC content: 28.79

Gene sequence:

>924_bases
ATGGAAAATAAAGATGGATTCTTAGTAATTAATAAAGATAAAGGCTGCACCTCTCATGATTGTGTTAAACAAATAAGGAA
GTTGTTAAATACAAGAAAAGTTGGACACACAGGAACTCTTGATCCAGAGGTTATAGGAACATTACCAATTGCAATAGGGA
GTGCAACAAGATTCATTCAATATCTTCCTCAGGGTAAAACTTATATTGGACAAATTAAATTAGGGATAAGAACTAACACT
GATGATATTCATGGAGAAATAATTAATCAAAAATGTTGGCCTAAAATCAGTGATGAGAAATTAGATCAATATTTAAATAA
ATTCAGAGGATTAATTAAACAAATTCCGCCAAAAGTATCTAGTGTTCATGTTAATGGTGAGAGAGCTTATAAAAAATCTT
TTAATAATGAAAATTTTGAATTAGCACCCAGAGAAGTAAAAATAGACGAACTCATTTTAATGAAATGGGACCAAATAAAC
GGAATCATAGAACTAAAAATCAATTGTTCTGCTGGTACATATATAAGGGCAATTGCAAGAGATTTAGGTGAAATTCTTAA
TTCCGAGGGGTGCCTTCTACAGCTAAAAAGAATTTCAGCTTGTGGATTTCATGAAAAAAACTCTATAAAAATATCTGATA
TTAAAAAAGGAATAGACCAAAAAAATGCGTCAAATTTTATTATTCCAACAATTTCTGCTCTTAATCACATTTCAACATTA
ATCTTAAATAAAGAAGAAGATATCAATTTTTGGCAAACTGGAAGAGCAATTAAATTTGATATTAATTACTTTAATGAAGG
TAACAATTTTGATAATAAAAAACCCATAAAGGTAGTAGATAAAAGAAAAATGCTACTCGGGATTGGCTTCTTAAATAAAG
AACTAACTAATATAAACCCAAAATTAGTCCTTAATGCAAAATAA

Upstream 100 bases:

>100_bases
AATATAGAACTATTATTTTTCAATTTCTTACTTTTAATTTCCGATATTAAAAATCATAGGATGGTAATAAATCTTTCTCA
AAGTATTCTTAATATTAAAA

Downstream 100 bases:

>100_bases
TTTTTATAGATAATCTTTTTTAAAAGGTTTAATCTGCACACCCCTGTAGTAATGCTTTAATATTTTTTCAGCTTTTTGGC
CTTTAGATGCCATATATTTA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MENKDGFLVINKDKGCTSHDCVKQIRKLLNTRKVGHTGTLDPEVIGTLPIAIGSATRFIQYLPQGKTYIGQIKLGIRTNT
DDIHGEIINQKCWPKISDEKLDQYLNKFRGLIKQIPPKVSSVHVNGERAYKKSFNNENFELAPREVKIDELILMKWDQIN
GIIELKINCSAGTYIRAIARDLGEILNSEGCLLQLKRISACGFHEKNSIKISDIKKGIDQKNASNFIIPTISALNHISTL
ILNKEEDINFWQTGRAIKFDINYFNEGNNFDNKKPIKVVDKRKMLLGIGFLNKELTNINPKLVLNAK

Sequences:

>Translated_307_residues
MENKDGFLVINKDKGCTSHDCVKQIRKLLNTRKVGHTGTLDPEVIGTLPIAIGSATRFIQYLPQGKTYIGQIKLGIRTNT
DDIHGEIINQKCWPKISDEKLDQYLNKFRGLIKQIPPKVSSVHVNGERAYKKSFNNENFELAPREVKIDELILMKWDQIN
GIIELKINCSAGTYIRAIARDLGEILNSEGCLLQLKRISACGFHEKNSIKISDIKKGIDQKNASNFIIPTISALNHISTL
ILNKEEDINFWQTGRAIKFDINYFNEGNNFDNKKPIKVVDKRKMLLGIGFLNKELTNINPKLVLNAK
>Mature_307_residues
MENKDGFLVINKDKGCTSHDCVKQIRKLLNTRKVGHTGTLDPEVIGTLPIAIGSATRFIQYLPQGKTYIGQIKLGIRTNT
DDIHGEIINQKCWPKISDEKLDQYLNKFRGLIKQIPPKVSSVHVNGERAYKKSFNNENFELAPREVKIDELILMKWDQIN
GIIELKINCSAGTYIRAIARDLGEILNSEGCLLQLKRISACGFHEKNSIKISDIKKGIDQKNASNFIIPTISALNHISTL
ILNKEEDINFWQTGRAIKFDINYFNEGNNFDNKKPIKVVDKRKMLLGIGFLNKELTNINPKLVLNAK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=217, Percent_Identity=31.3364055299539, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4503337, Length=207, Percent_Identity=28.0193236714976, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI215599015, Length=207, Percent_Identity=28.0193236714976, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI2367200, Length=234, Percent_Identity=31.6239316239316, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17553978, Length=193, Percent_Identity=31.6062176165803, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323204, Length=210, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324037, Length=132, Percent_Identity=32.5757575757576, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281364189, Length=201, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364187, Length=201, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364185, Length=201, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364183, Length=201, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI62471759, Length=201, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17975520, Length=201, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PROM9 (Q319E0)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397941.1
- ProteinModelPortal:   Q319E0
- SMR:   Q319E0
- STRING:   Q319E0
- GeneID:   3766261
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1445
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   CLSK2516628
- BioCyc:   PMAR74546:PMT9312_1445-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34773; Mature: 34773

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: ACT_SITE 41-41

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKDGFLVINKDKGCTSHDCVKQIRKLLNTRKVGHTGTLDPEVIGTLPIAIGSATRFIQ
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEEHHCHHHHHHH
YLPQGKTYIGQIKLGIRTNTDDIHGEIINQKCWPKISDEKLDQYLNKFRGLIKQIPPKVS
HCCCCCEEEEEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
SVHVNGERAYKKSFNNENFELAPREVKIDELILMKWDQINGIIELKINCSAGTYIRAIAR
EEEECCHHHHHHCCCCCCCEECCCEECCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHH
DLGEILNSEGCLLQLKRISACGFHEKNSIKISDIKKGIDQKNASNFIIPTISALNHISTL
HHHHHHCCCCCEEEEEHHHHCCCCCCCCEEHHHHHHCCCCCCCCCEEEEHHHHHHHHHHH
ILNKEEDINFWQTGRAIKFDINYFNEGNNFDNKKPIKVVDKRKMLLGIGFLNKELTNINP
HCCCCCCCCHHHCCCEEEEEEEEECCCCCCCCCCCCHHHCCCEEEEEEEHHHHHHHCCCC
KLVLNAK
EEEEECC
>Mature Secondary Structure
MENKDGFLVINKDKGCTSHDCVKQIRKLLNTRKVGHTGTLDPEVIGTLPIAIGSATRFIQ
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEEHHCHHHHHHH
YLPQGKTYIGQIKLGIRTNTDDIHGEIINQKCWPKISDEKLDQYLNKFRGLIKQIPPKVS
HCCCCCEEEEEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
SVHVNGERAYKKSFNNENFELAPREVKIDELILMKWDQINGIIELKINCSAGTYIRAIAR
EEEECCHHHHHHCCCCCCCEECCCEECCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHH
DLGEILNSEGCLLQLKRISACGFHEKNSIKISDIKKGIDQKNASNFIIPTISALNHISTL
HHHHHHCCCCCEEEEEHHHHCCCCCCCCEEHHHHHHCCCCCCCCCEEEEHHHHHHHHHHH
ILNKEEDINFWQTGRAIKFDINYFNEGNNFDNKKPIKVVDKRKMLLGIGFLNKELTNINP
HCCCCCCCCHHHCCCEEEEEEEEECCCCCCCCCCCCHHHCCCEEEEEEEHHHHHHHCCCC
KLVLNAK
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA