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The map label for this gene is glgX [H]

Identifier: 78779804

GI number: 78779804

Start: 1332760

End: 1334793

Strand: Direct

Name: glgX [H]

Synonym: PMT9312_1420

Alternate gene names: 78779804

Gene position: 1332760-1334793 (Clockwise)

Preceding gene: 78779799

Following gene: 78779805

Centisome position: 77.98

GC content: 31.32

Gene sequence:

>2034_bases
GTGACTCATATCAATAAAGGTAACCCATTTCCTCTAGGAAGTTCTCTAACTGCAAAAGGAGTTAATTTTTCCCTAATAGC
CACAAATGCAGCATTTGTAGAAATCTTGTTGTTTGAGAAAGAAGACTCTATTTCCCCAAAAAACATATTTAAACTAGATC
ATAATCATAACACTGGTCCCTACTGGCATGCTGAAATAGAAAATCTTAATGAAGGTTGTATTTATGCTTTTAGAATAAAT
CAAAAAAATAATGGGATTAACAATAATTACGAAAAAAAGGTTTTACTTGATCCATGTTCAAGGGGTATTACTGGATGGGG
AATTTATAAAAGAGAAAATGCATTAAATACGAATGACAATACTAATTCTTGTCTTAAAAGCGTAGTTTGCGACAGAAAAT
TATTTAATTTTGAAGATTATCCAAGACCAAAACATTCTTGGGAAGAAACAATTATTTATGAACTCCATATAAGAGCCTTT
ACTGAGTCAACGGATAAAGATAAAAGTTGTTTTAAAAAATTTTTGAAAAAAATTCCGTATCTCAAGGAACTTGGTATTAC
AACAATTGAATTGCTTCCAGTTTTTTGTTTTGATCCAACTGATGCACCAAATGGTTTAGAAAATTTTTGGGGTTATAGTC
CAATCAATTGGTTTACACCGCATTTTGAATATCTTTCGAATGAGTCTGCAGAAAAGAATAGAGAGGAATTTAGAAGATTA
GTAGAGGAATGTCATAAAGCAGACATTGAAGTAATCTTAGATGTTGTATACAATCACACCTCTGAGGGAGATTCCCAAGG
ACCTGTAATATCTTGGAAAGGTATAGATGAAAATCTTTATTACTTTATTGGAAAAGATAAAAATTATCAGGACGTCTCTG
GGTGCGGTAATACTATTGCAGCAAACAGAGGATTAGTTAGAAAACTAATAATTGAATCATTAAAATGTTGGGCGAGTGAA
TTAGGAGTTGATGGTTTTAGATTTGATTTAGGAATTGCCCTATCAAGAGGAGAAAATCTTTCGCCACTAGATAATCCTCC
AATTTTTGAAGATATAGAATGTGAACCAGAACTTATTGATATCAAATTCATAAGTGAACCATGGGATTGTGGTGGTTTAT
ATAAATTAGGTGATTTCCCATCTAAGACTACTTTTACTTGGAATGGCCATTTTAGAGATGATTTGCGAAGATTTTGGAAG
GGAGATAAAGATACAGCTTGGAATATGAGCGATAAAATAAAAGGTACTCCATCTATGTATAAAGAAGATAATATTTTCCC
AAAATCAATAAATTTTATTACTTCACATGATGGATTCACTCTAAAAGATTTAGTAACATTCAATAGAAAACATAATTTTG
CTAATAGAGAACAAAATAGAGATGGAGATAACCATAACAATTCTTGGAATCATGGTACGGAGGGACCAACTAAAAACTTA
TTAATCAATGATTTAAGAAAAAGACAACAAAGAAATCTTATTCTTAGTTTACTTATCTCTAAAGGTGTTCCAATGATTCT
AATGGGTGATGAAATAGGAAGGTCCCAAGGAGGCAACAATAATTCTTGGTGCCAAAATAATTTATTGGGTTGGATGAATT
GGGATCATGATCAACAAGATTTGGAATTATTAGAATATTCTAAATACGTTATAAAAATTCGAAAAAAACTAATAAACATT
TTTAATCCATCATTCTTACCTAACAATCAAAACCAAAAAAATATTCCAATATATCATTGGCATGGAACAAAGTTAGATAG
CCCCGACTGGAGTAGTTGGTCTCACACAGTTGCCTTTAGCATAAACAAAGGCAAAACTAATCCTCTGGTCTGGATAGGTT
TAAATGCATATTCAAAAAGTATCGATTTCCCCTTACCAAAATGTAAATATAATTGGTTAAAAGTTATTGATACCAGCGCA
CCTGAGATTTTCGAACCCTTAACAATTAATGAAAAATCTGTTTCAATAAAGAGTAGAAGCTCTTTATTAATCATTTCGGA
AGAAGTATTTGGGGCAAAAAATAATATATTCTAA

Upstream 100 bases:

>100_bases
CTTAAATTTTAGTTTTTTTTTAGATTTTGCATCTATTATTCAAATATCAGTAATTTGAATAAATTATCTCAAAATTTAAT
TCAGCAATGATAAAGAGACA

Downstream 100 bases:

>100_bases
AAGCGGGCGGCGGGAATCGAACCCGCATCTTCAGCTTGGAAGGCTGAGGTTTTACCACTAAACCACGCCCGCATTAAGTA
ATAGAATTACCATTGCAATA

Product: alpha amylase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MTHINKGNPFPLGSSLTAKGVNFSLIATNAAFVEILLFEKEDSISPKNIFKLDHNHNTGPYWHAEIENLNEGCIYAFRIN
QKNNGINNNYEKKVLLDPCSRGITGWGIYKRENALNTNDNTNSCLKSVVCDRKLFNFEDYPRPKHSWEETIIYELHIRAF
TESTDKDKSCFKKFLKKIPYLKELGITTIELLPVFCFDPTDAPNGLENFWGYSPINWFTPHFEYLSNESAEKNREEFRRL
VEECHKADIEVILDVVYNHTSEGDSQGPVISWKGIDENLYYFIGKDKNYQDVSGCGNTIAANRGLVRKLIIESLKCWASE
LGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELIDIKFISEPWDCGGLYKLGDFPSKTTFTWNGHFRDDLRRFWK
GDKDTAWNMSDKIKGTPSMYKEDNIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGTEGPTKNL
LINDLRKRQQRNLILSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWDHDQQDLELLEYSKYVIKIRKKLINI
FNPSFLPNNQNQKNIPIYHWHGTKLDSPDWSSWSHTVAFSINKGKTNPLVWIGLNAYSKSIDFPLPKCKYNWLKVIDTSA
PEIFEPLTINEKSVSIKSRSSLLIISEEVFGAKNNIF

Sequences:

>Translated_677_residues
MTHINKGNPFPLGSSLTAKGVNFSLIATNAAFVEILLFEKEDSISPKNIFKLDHNHNTGPYWHAEIENLNEGCIYAFRIN
QKNNGINNNYEKKVLLDPCSRGITGWGIYKRENALNTNDNTNSCLKSVVCDRKLFNFEDYPRPKHSWEETIIYELHIRAF
TESTDKDKSCFKKFLKKIPYLKELGITTIELLPVFCFDPTDAPNGLENFWGYSPINWFTPHFEYLSNESAEKNREEFRRL
VEECHKADIEVILDVVYNHTSEGDSQGPVISWKGIDENLYYFIGKDKNYQDVSGCGNTIAANRGLVRKLIIESLKCWASE
LGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELIDIKFISEPWDCGGLYKLGDFPSKTTFTWNGHFRDDLRRFWK
GDKDTAWNMSDKIKGTPSMYKEDNIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGTEGPTKNL
LINDLRKRQQRNLILSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWDHDQQDLELLEYSKYVIKIRKKLINI
FNPSFLPNNQNQKNIPIYHWHGTKLDSPDWSSWSHTVAFSINKGKTNPLVWIGLNAYSKSIDFPLPKCKYNWLKVIDTSA
PEIFEPLTINEKSVSIKSRSSLLIISEEVFGAKNNIF
>Mature_676_residues
THINKGNPFPLGSSLTAKGVNFSLIATNAAFVEILLFEKEDSISPKNIFKLDHNHNTGPYWHAEIENLNEGCIYAFRINQ
KNNGINNNYEKKVLLDPCSRGITGWGIYKRENALNTNDNTNSCLKSVVCDRKLFNFEDYPRPKHSWEETIIYELHIRAFT
ESTDKDKSCFKKFLKKIPYLKELGITTIELLPVFCFDPTDAPNGLENFWGYSPINWFTPHFEYLSNESAEKNREEFRRLV
EECHKADIEVILDVVYNHTSEGDSQGPVISWKGIDENLYYFIGKDKNYQDVSGCGNTIAANRGLVRKLIIESLKCWASEL
GVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELIDIKFISEPWDCGGLYKLGDFPSKTTFTWNGHFRDDLRRFWKG
DKDTAWNMSDKIKGTPSMYKEDNIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGTEGPTKNLL
INDLRKRQQRNLILSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWDHDQQDLELLEYSKYVIKIRKKLINIF
NPSFLPNNQNQKNIPIYHWHGTKLDSPDWSSWSHTVAFSINKGKTNPLVWIGLNAYSKSIDFPLPKCKYNWLKVIDTSAP
EIFEPLTINEKSVSIKSRSSLLIISEEVFGAKNNIF

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=575, Percent_Identity=37.3913043478261, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77949; Mature: 77817

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHINKGNPFPLGSSLTAKGVNFSLIATNAAFVEILLFEKEDSISPKNIFKLDHNHNTGP
CCCCCCCCCCCCCCCCEECCCEEEEEECCCEEEEEEEEECCCCCCCCCEEEECCCCCCCC
YWHAEIENLNEGCIYAFRINQKNNGINNNYEKKVLLDPCSRGITGWGIYKRENALNTNDN
EEEEECCCCCCCEEEEEEEECCCCCCCCCCCCEEEECHHHCCCCCCEEEEECCCCCCCCC
TNSCLKSVVCDRKLFNFEDYPRPKHSWEETIIYELHIRAFTESTDKDKSCFKKFLKKIPY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCC
LKELGITTIELLPVFCFDPTDAPNGLENFWGYSPINWFTPHFEYLSNESAEKNREEFRRL
HHHCCCEEEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
VEECHKADIEVILDVVYNHTSEGDSQGPVISWKGIDENLYYFIGKDKNYQDVSGCGNTIA
HHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHCCCCCHH
ANRGLVRKLIIESLKCWASELGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELID
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
IKFISEPWDCGGLYKLGDFPSKTTFTWNGHFRDDLRRFWKGDKDTAWNMSDKIKGTPSMY
EEEECCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KEDNIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGTEGPTKNL
CCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LINDLRKRQQRNLILSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWDHDQQD
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCHHHCCCCCCCCCCCHHH
LELLEYSKYVIKIRKKLINIFNPSFLPNNQNQKNIPIYHWHGTKLDSPDWSSWSHTVAFS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEE
INKGKTNPLVWIGLNAYSKSIDFPLPKCKYNWLKVIDTSAPEIFEPLTINEKSVSIKSRS
ECCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCEEEEECCC
SLLIISEEVFGAKNNIF
CEEEEEHHHCCCCCCCC
>Mature Secondary Structure 
THINKGNPFPLGSSLTAKGVNFSLIATNAAFVEILLFEKEDSISPKNIFKLDHNHNTGP
CCCCCCCCCCCCCCCEECCCEEEEEECCCEEEEEEEEECCCCCCCCCEEEECCCCCCCC
YWHAEIENLNEGCIYAFRINQKNNGINNNYEKKVLLDPCSRGITGWGIYKRENALNTNDN
EEEEECCCCCCCEEEEEEEECCCCCCCCCCCCEEEECHHHCCCCCCEEEEECCCCCCCCC
TNSCLKSVVCDRKLFNFEDYPRPKHSWEETIIYELHIRAFTESTDKDKSCFKKFLKKIPY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCC
LKELGITTIELLPVFCFDPTDAPNGLENFWGYSPINWFTPHFEYLSNESAEKNREEFRRL
HHHCCCEEEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
VEECHKADIEVILDVVYNHTSEGDSQGPVISWKGIDENLYYFIGKDKNYQDVSGCGNTIA
HHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHCCCCCHH
ANRGLVRKLIIESLKCWASELGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELID
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
IKFISEPWDCGGLYKLGDFPSKTTFTWNGHFRDDLRRFWKGDKDTAWNMSDKIKGTPSMY
EEEECCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KEDNIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGTEGPTKNL
CCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LINDLRKRQQRNLILSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWDHDQQD
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCHHHCCCCCCCCCCCHHH
LELLEYSKYVIKIRKKLINIFNPSFLPNNQNQKNIPIYHWHGTKLDSPDWSSWSHTVAFS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEE
INKGKTNPLVWIGLNAYSKSIDFPLPKCKYNWLKVIDTSAPEIFEPLTINEKSVSIKSRS
ECCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCEEEEECCC
SLLIISEEVFGAKNNIF
CEEEEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]