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The map label for this gene is asnB [H]

Identifier: 78779707

GI number: 78779707

Start: 1236769

End: 1238637

Strand: Direct

Name: asnB [H]

Synonym: PMT9312_1324

Alternate gene names: 78779707

Gene position: 1236769-1238637 (Clockwise)

Preceding gene: 78779706

Following gene: 78779708

Centisome position: 72.36

GC content: 25.74

Gene sequence:

>1869_bases
ATGTGTGGTATAACAGGAATTATCTCATTTCAAAGTAGTATAGATCCTCAAAATATTCACAATATGAATGAGACTCTCAT
GCATAGAGGGCCTGATCACAGAGACATATGGATTAATGAATATAGTACTGTTTTCCTTGGTCATACAAGATTATCCATAT
TAGATTTATCTGACAATGGGAATCAACCTTTTCATAGTAAAGATGGTAGATTTCACTTAACTTTTAACGGTGAAATATAC
AATTATAAAAATCTTAAAGATTATCTCAAAACAAATGGGATTTCAAATTTCAAAAGTAATTCAGATACAGAAGTATTAAT
TGAACTTATAGCTTTACGTGGAATAACAAATGCTTCAAAAATGCTTAATGGAATGTTTGCATTTGCAGTTTGGGACTCTT
TAGAAAAAAAAATACATCTTGTAAGAGATAGATTTGGTGAAAAACCATTATTTTATACTCTCCAAAAAGATACTTTATAT
TTTGGATCAGAAATTAAATCAATATTAAAAATACCTACAATATCAAAAATAATTAATAAAGAAGCTTTGTCTCATTATCT
GAGGTTTAACTACATTAAAAATCCATTAACAATATATAAAGATATTTATAAATTAGAGCCAGGCCACATTTTAAGCATTG
ATCAAAATATTACTTACAAAAAATACAGTTACAAAAATAAAAATAAAAAAACAATCAAATTAGCTAATCAAAAAGAAGCA
GAAGATTTACTACAAAATCAAATTATAAAAAGTTGTTTGTCAAGAACAATATCGGATGTTCCCATTGCTTGTTTTTTATC
GGGAGGCATAGATTCTTCTTTAGTTACAAGTATTATTCAAAGGAACTCAAAACAAAGAATTAAAACTTTCACTATCGGTT
TTGATGATCAATCTATAGATGAATCTAATTATGCTGAAAAAATAGCAAATTTTTTGGGAACTGATCATACGACTATAAAT
ATTGAAGAAAAAGAATTATTAAATAATATTGATTTACAAAGTGAGATTTATGGAGAACCGTTTGCAGATCAATCATCCAT
TGCAACAAACATTCTATGTAAATATGCTTCAAGGGATGTCAAAGTATGTCTTAGTGGAGATGGCGGAGATGAAATGTTTA
TTGGGTACGATAGATACTACAAAGTAAACCAATTAAGGTTATTCCTAAAAGCAATAAAATTTTATAAAAAATTTAAAACA
ATTGATAGAATTTTGGAAGTTTTGATTCACAAGATTGAAAGTAATAAAAACTTTTCTATAAAGATTGACAAGATAATTAA
ATTAATCAGTCTATTATCAGATGCAAATGATGATAATATTTATGAAAAATTCAACTCATTATATAGAGATCAATATTTCC
CATTAACTCCTCAGAATAATCAAAAACATTATATATATAAAGATTTAAAAAATATTCAAGAAATGTGTGAATATGATTTT
GAAACTTATCTACCTGATTGTATTTTAACTAAAGTTGATAGAGCAAGTATGCATAATTCATTAGAAGTAAGGGCCCCTTT
TCTTGACCATGAAATTCCTGAAATTATTGACTCTATTAATCCTACATATAAAAAAAAATTACTAAATAGAAAATTCATAT
TAAAAAAATTATTGAATAAATATCTTCCCAAAAATATATATGAAAGGCCAAAAAAAGGATTTAGCGCTCCTCTTAATAAT
TGGATAAAAGGTCCACTCAAAGAACTTACTTTAGATTATTTATCTATTGAATCTTTAAAGAAACATAATTTATTAGATAT
TGATATTATTGAAAGAATCAAATATGAACATTATTCTGGGATAAGGTCTTGGCATAATCAAATATGGAACCTTTTGATTT
TTCAACAATGGTATAATAAAAATTTTTAA

Upstream 100 bases:

>100_bases
TAGTATTAAATCAAAAATGGCTAGAGTTAGAATTAAAAATAACTTTTCACAAGAAAATATGATTAAAAAATACCAAGAGC
TTTATTCCAAAATTTAAATC

Downstream 100 bases:

>100_bases
ATTTATAAGTTCTAAACTATTTTTTATTATAAAAATTATTTAAAAACAAATTAAATATAGTTACATTTAAGTAAATTCCA
AACTCCAACATGGGTTAATT

Product: asparagine synthase (glutamine-hydrolysing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MCGITGIISFQSSIDPQNIHNMNETLMHRGPDHRDIWINEYSTVFLGHTRLSILDLSDNGNQPFHSKDGRFHLTFNGEIY
NYKNLKDYLKTNGISNFKSNSDTEVLIELIALRGITNASKMLNGMFAFAVWDSLEKKIHLVRDRFGEKPLFYTLQKDTLY
FGSEIKSILKIPTISKIINKEALSHYLRFNYIKNPLTIYKDIYKLEPGHILSIDQNITYKKYSYKNKNKKTIKLANQKEA
EDLLQNQIIKSCLSRTISDVPIACFLSGGIDSSLVTSIIQRNSKQRIKTFTIGFDDQSIDESNYAEKIANFLGTDHTTIN
IEEKELLNNIDLQSEIYGEPFADQSSIATNILCKYASRDVKVCLSGDGGDEMFIGYDRYYKVNQLRLFLKAIKFYKKFKT
IDRILEVLIHKIESNKNFSIKIDKIIKLISLLSDANDDNIYEKFNSLYRDQYFPLTPQNNQKHYIYKDLKNIQEMCEYDF
ETYLPDCILTKVDRASMHNSLEVRAPFLDHEIPEIIDSINPTYKKKLLNRKFILKKLLNKYLPKNIYERPKKGFSAPLNN
WIKGPLKELTLDYLSIESLKKHNLLDIDIIERIKYEHYSGIRSWHNQIWNLLIFQQWYNKNF

Sequences:

>Translated_622_residues
MCGITGIISFQSSIDPQNIHNMNETLMHRGPDHRDIWINEYSTVFLGHTRLSILDLSDNGNQPFHSKDGRFHLTFNGEIY
NYKNLKDYLKTNGISNFKSNSDTEVLIELIALRGITNASKMLNGMFAFAVWDSLEKKIHLVRDRFGEKPLFYTLQKDTLY
FGSEIKSILKIPTISKIINKEALSHYLRFNYIKNPLTIYKDIYKLEPGHILSIDQNITYKKYSYKNKNKKTIKLANQKEA
EDLLQNQIIKSCLSRTISDVPIACFLSGGIDSSLVTSIIQRNSKQRIKTFTIGFDDQSIDESNYAEKIANFLGTDHTTIN
IEEKELLNNIDLQSEIYGEPFADQSSIATNILCKYASRDVKVCLSGDGGDEMFIGYDRYYKVNQLRLFLKAIKFYKKFKT
IDRILEVLIHKIESNKNFSIKIDKIIKLISLLSDANDDNIYEKFNSLYRDQYFPLTPQNNQKHYIYKDLKNIQEMCEYDF
ETYLPDCILTKVDRASMHNSLEVRAPFLDHEIPEIIDSINPTYKKKLLNRKFILKKLLNKYLPKNIYERPKKGFSAPLNN
WIKGPLKELTLDYLSIESLKKHNLLDIDIIERIKYEHYSGIRSWHNQIWNLLIFQQWYNKNF
>Mature_622_residues
MCGITGIISFQSSIDPQNIHNMNETLMHRGPDHRDIWINEYSTVFLGHTRLSILDLSDNGNQPFHSKDGRFHLTFNGEIY
NYKNLKDYLKTNGISNFKSNSDTEVLIELIALRGITNASKMLNGMFAFAVWDSLEKKIHLVRDRFGEKPLFYTLQKDTLY
FGSEIKSILKIPTISKIINKEALSHYLRFNYIKNPLTIYKDIYKLEPGHILSIDQNITYKKYSYKNKNKKTIKLANQKEA
EDLLQNQIIKSCLSRTISDVPIACFLSGGIDSSLVTSIIQRNSKQRIKTFTIGFDDQSIDESNYAEKIANFLGTDHTTIN
IEEKELLNNIDLQSEIYGEPFADQSSIATNILCKYASRDVKVCLSGDGGDEMFIGYDRYYKVNQLRLFLKAIKFYKKFKT
IDRILEVLIHKIESNKNFSIKIDKIIKLISLLSDANDDNIYEKFNSLYRDQYFPLTPQNNQKHYIYKDLKNIQEMCEYDF
ETYLPDCILTKVDRASMHNSLEVRAPFLDHEIPEIIDSINPTYKKKLLNRKFILKKLLNKYLPKNIYERPKKGFSAPLNN
WIKGPLKELTLDYLSIESLKKHNLLDIDIIERIKYEHYSGIRSWHNQIWNLLIFQQWYNKNF

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=405, Percent_Identity=28.1481481481481, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI168229252, Length=405, Percent_Identity=28.1481481481481, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI168229250, Length=405, Percent_Identity=28.1481481481481, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI296010848, Length=380, Percent_Identity=28.4210526315789, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI296010852, Length=308, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI296010850, Length=308, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786889, Length=404, Percent_Identity=27.4752475247525, Blast_Score=122, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71993933, Length=491, Percent_Identity=27.4949083503055, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25147557, Length=491, Percent_Identity=27.4949083503055, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560178, Length=388, Percent_Identity=30.1546391752577, Blast_Score=116, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321563, Length=423, Percent_Identity=26.4775413711584, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=417, Percent_Identity=26.6187050359712, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45553209, Length=406, Percent_Identity=29.5566502463054, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72765; Mature: 72765

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGIISFQSSIDPQNIHNMNETLMHRGPDHRDIWINEYSTVFLGHTRLSILDLSDNG
CCCCCEEEEHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCEEEEEEECCCC
NQPFHSKDGRFHLTFNGEIYNYKNLKDYLKTNGISNFKSNSDTEVLIELIALRGITNASK
CCCCCCCCCEEEEEECCEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
MLNGMFAFAVWDSLEKKIHLVRDRFGEKPLFYTLQKDTLYFGSEIKSILKIPTISKIINK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHH
EALSHYLRFNYIKNPLTIYKDIYKLEPGHILSIDQNITYKKYSYKNKNKKTIKLANQKEA
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCCCEEEECCHHHH
EDLLQNQIIKSCLSRTISDVPIACFLSGGIDSSLVTSIIQRNSKQRIKTFTIGFDDQSID
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCC
ESNYAEKIANFLGTDHTTINIEEKELLNNIDLQSEIYGEPFADQSSIATNILCKYASRDV
CHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCE
KVCLSGDGGDEMFIGYDRYYKVNQLRLFLKAIKFYKKFKTIDRILEVLIHKIESNKNFSI
EEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
KIDKIIKLISLLSDANDDNIYEKFNSLYRDQYFPLTPQNNQKHYIYKDLKNIQEMCEYDF
EHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
ETYLPDCILTKVDRASMHNSLEVRAPFLDHEIPEIIDSINPTYKKKLLNRKFILKKLLNK
HHHCCHHHHHHHHHHHHCCCCEEECCHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YLPKNIYERPKKGFSAPLNNWIKGPLKELTLDYLSIESLKKHNLLDIDIIERIKYEHYSG
HCCHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IRSWHNQIWNLLIFQQWYNKNF
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCGITGIISFQSSIDPQNIHNMNETLMHRGPDHRDIWINEYSTVFLGHTRLSILDLSDNG
CCCCCEEEEHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCEEEEEEECCCC
NQPFHSKDGRFHLTFNGEIYNYKNLKDYLKTNGISNFKSNSDTEVLIELIALRGITNASK
CCCCCCCCCEEEEEECCEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
MLNGMFAFAVWDSLEKKIHLVRDRFGEKPLFYTLQKDTLYFGSEIKSILKIPTISKIINK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHH
EALSHYLRFNYIKNPLTIYKDIYKLEPGHILSIDQNITYKKYSYKNKNKKTIKLANQKEA
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCCCEEEECCHHHH
EDLLQNQIIKSCLSRTISDVPIACFLSGGIDSSLVTSIIQRNSKQRIKTFTIGFDDQSID
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCC
ESNYAEKIANFLGTDHTTINIEEKELLNNIDLQSEIYGEPFADQSSIATNILCKYASRDV
CHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCE
KVCLSGDGGDEMFIGYDRYYKVNQLRLFLKAIKFYKKFKTIDRILEVLIHKIESNKNFSI
EEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
KIDKIIKLISLLSDANDDNIYEKFNSLYRDQYFPLTPQNNQKHYIYKDLKNIQEMCEYDF
EHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
ETYLPDCILTKVDRASMHNSLEVRAPFLDHEIPEIIDSINPTYKKKLLNRKFILKKLLNK
HHHCCHHHHHHHHHHHHCCCCEEECCHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YLPKNIYERPKKGFSAPLNNWIKGPLKELTLDYLSIESLKKHNLLDIDIIERIKYEHYSG
HCCHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IRSWHNQIWNLLIFQQWYNKNF
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]