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The map label for this gene is lipA2 [H]

Identifier: 78779490

GI number: 78779490

Start: 1020099

End: 1020998

Strand: Reverse

Name: lipA2 [H]

Synonym: PMT9312_1107

Alternate gene names: 78779490

Gene position: 1020998-1020099 (Counterclockwise)

Preceding gene: 78779491

Following gene: 78779483

Centisome position: 59.74

GC content: 32.11

Gene sequence:

>900_bases
ATGAGAGACAATAATCTAATCAAGAAAGAAAAAATCTTAAGACTTCCCTCTTGGATTAAATTTCCTATTAGTAAAGCTTC
AGAATTCGAAAAAATACAAACACTCATCAAAAAATCAAATTTTCATACTATTTGTGAAGAAGCAAGATGTCCTAATAGAG
CAGAATGTTATGCCTCAGGAACAGCCACTTTCTTACTGGGTGGATCGATATGTTCTCGTTCATGTGCTTTTTGTCAGGTA
AATAAAGGTAGACCTAGTTCTGTCAATATTGATGAATGTACTCAAGTCGCTGAAGCAGTGAAAGTACTAAATTTAAAATA
TGTTGTTTTGACATCTGTAGCTAGAGACGATCTCCCTGATCATGGTGCAAATTTATTTATATCTACAATTGATGAGATCA
GAAAAATTGATTCAACAATTAAAATAGAGGTTTTAACTCCTGATTTATGGGGTGGGGGCAAAAATTTTGATGAAACTAAT
AACCTTCAGACTGAGAGATTGAAGATGATTTTAGAAAAAGATCCAATATGCTTTAATCATAACCTTGAAACTGTTGAAAG
ACTGCAAAAAGAAGTTAGGAGGGGTGCAAATTACAAAAAATCCCTTAGTTTACTAAAAAAGTCAAAAGATATTGCTCCTC
ATATTCAAACTAAATCAGGCATTATGTTAGGTCTTGGTGAAACATTGGATGAAATAAAAAATACAATTCATGATCTTAAA
AAAATAGATTGTGATCAAATTACAATTGGCCAATATTTAAGGCCCTCATTCAATCATTTGGAAGTTAAGAAATATTGGGA
TCCATCAGAGTTTGAATATTTATATCGCTTCTCTAAGGAATTAGGATTCAAAAAAGTATCTTCTGGCCCTTTAGTTAGAA
GCAGTTATCATGCGGGTTAA

Upstream 100 bases:

>100_bases
GAGGATTGCATATGGCCTACCAATGGGCAGTGAACTTGAATATGTAGATGAAGTTACACTTGCAAGGGCGTTAGAAGGAA
GAACAAAACTTAATTAGACA

Downstream 100 bases:

>100_bases
CTTTTTTCAATTAAAGAGAGTTTCTTTTCAAATTTTTTCAATATGGAAATACATTCCCCGTTCATAAGTGTACCTGCACC
TCCCCAAACCAATCTTAATA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRDNNLIKKEKILRLPSWIKFPISKASEFEKIQTLIKKSNFHTICEEARCPNRAECYASGTATFLLGGSICSRSCAFCQV
NKGRPSSVNIDECTQVAEAVKVLNLKYVVLTSVARDDLPDHGANLFISTIDEIRKIDSTIKIEVLTPDLWGGGKNFDETN
NLQTERLKMILEKDPICFNHNLETVERLQKEVRRGANYKKSLSLLKKSKDIAPHIQTKSGIMLGLGETLDEIKNTIHDLK
KIDCDQITIGQYLRPSFNHLEVKKYWDPSEFEYLYRFSKELGFKKVSSGPLVRSSYHAG

Sequences:

>Translated_299_residues
MRDNNLIKKEKILRLPSWIKFPISKASEFEKIQTLIKKSNFHTICEEARCPNRAECYASGTATFLLGGSICSRSCAFCQV
NKGRPSSVNIDECTQVAEAVKVLNLKYVVLTSVARDDLPDHGANLFISTIDEIRKIDSTIKIEVLTPDLWGGGKNFDETN
NLQTERLKMILEKDPICFNHNLETVERLQKEVRRGANYKKSLSLLKKSKDIAPHIQTKSGIMLGLGETLDEIKNTIHDLK
KIDCDQITIGQYLRPSFNHLEVKKYWDPSEFEYLYRFSKELGFKKVSSGPLVRSSYHAG
>Mature_299_residues
MRDNNLIKKEKILRLPSWIKFPISKASEFEKIQTLIKKSNFHTICEEARCPNRAECYASGTATFLLGGSICSRSCAFCQV
NKGRPSSVNIDECTQVAEAVKVLNLKYVVLTSVARDDLPDHGANLFISTIDEIRKIDSTIKIEVLTPDLWGGGKNFDETN
NLQTERLKMILEKDPICFNHNLETVERLQKEVRRGANYKKSLSLLKKSKDIAPHIQTKSGIMLGLGETLDEIKNTIHDLK
KIDCDQITIGQYLRPSFNHLEVKKYWDPSEFEYLYRFSKELGFKKVSSGPLVRSSYHAG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=299, Percent_Identity=40.8026755852843, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI37577164, Length=262, Percent_Identity=39.3129770992366, Blast_Score=189, Evalue=4e-48,
Organism=Escherichia coli, GI1786846, Length=296, Percent_Identity=42.5675675675676, Blast_Score=254, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI32564533, Length=304, Percent_Identity=40.1315789473684, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=42.0875420875421, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI221513272, Length=298, Percent_Identity=42.2818791946309, Blast_Score=233, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34004; Mature: 34004

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDNNLIKKEKILRLPSWIKFPISKASEFEKIQTLIKKSNFHTICEEARCPNRAECYASG
CCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHEEECC
TATFLLGGSICSRSCAFCQVNKGRPSSVNIDECTQVAEAVKVLNLKYVVLTSVARDDLPD
CEEEEECCHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HGANLFISTIDEIRKIDSTIKIEVLTPDLWGGGKNFDETNNLQTERLKMILEKDPICFNH
CCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECC
NLETVERLQKEVRRGANYKKSLSLLKKSKDIAPHIQTKSGIMLGLGETLDEIKNTIHDLK
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
KIDCDQITIGQYLRPSFNHLEVKKYWDPSEFEYLYRFSKELGFKKVSSGPLVRSSYHAG
HCCCCCEEHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRDNNLIKKEKILRLPSWIKFPISKASEFEKIQTLIKKSNFHTICEEARCPNRAECYASG
CCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHEEECC
TATFLLGGSICSRSCAFCQVNKGRPSSVNIDECTQVAEAVKVLNLKYVVLTSVARDDLPD
CEEEEECCHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HGANLFISTIDEIRKIDSTIKIEVLTPDLWGGGKNFDETNNLQTERLKMILEKDPICFNH
CCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECC
NLETVERLQKEVRRGANYKKSLSLLKKSKDIAPHIQTKSGIMLGLGETLDEIKNTIHDLK
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
KIDCDQITIGQYLRPSFNHLEVKKYWDPSEFEYLYRFSKELGFKKVSSGPLVRSSYHAG
HCCCCCEEHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]