Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 78779460

GI number: 78779460

Start: 987312

End: 988952

Strand: Direct

Name: leuA [H]

Synonym: PMT9312_1077

Alternate gene names: 78779460

Gene position: 987312-988952 (Clockwise)

Preceding gene: 78779458

Following gene: 78779461

Centisome position: 57.76

GC content: 35.83

Gene sequence:

>1641_bases
ATGTCCAAAGATCCTGGAAGAATTTTGATCTTTGATACAACTCTTCGAGATGGAGAGCAATCTCCTGGTGCCAGTTTAAA
TCTTGAAGAAAAGCTTGCTATTGCACATCAATTAGCAAGATTAGGAGTAGATGTTATTGAGGCTGGATTCCCTTTCGCAA
GTCCCGGAGATTTTAAAGCTGTTAATAAAATTTCCAATGATGTAGGGAAAGAAAACGGTCCTATAATATGCGGTTTAGCT
AGAGCGTCCAAAGGAGATATAAAAGCATGTTATGAAGCAGTAAGTCCAGCCCCAAAGAAAAGAATACATACTTTTATTGC
CACAAGCGATATTCACCTTAAACATAAACTTAAAAAGTCCAGAAAAGATGTCCTTCAAATAGTTCCAGAAATGGTTAATT
ATGCAAAAACATTGGTAGAAGATATTGAGTTTTCTTGTGAAGATGCTTCAAGGAGTGATCCAGATTTTTTATACGAAGTG
ATTCAATTAGCAATTTCTGCAGGTGCGACAACTATAAATATCCCTGATACTGTTGGATTTACAACTCCTAGTGAATTTGG
CAAATTAATTTCTGATATAAATAAAAATGTTCCAAATATTGATGAAGCAGTAATATCTGTTCATGGTCATAATGATTTAG
GTCTAGCAGTTGCCAATTTTCTAGAGGCAGTAAAAAATGGAGCGAGACAACTAGAATGTACGATTAATGGAATTGGTGAA
AGAGCAGGAAATGCCTCTCTCGAAGAATTAGTAATGGCGCTGCATGTAAGGAAAAGTTTTTTTAATAGTTTTTTTAAAAG
AAATCCAGATTCGCCAACTCCTCTTACTGCCATAAGAACAGAAGAGATAACAAAAACTTCTAGGCTCGTTTCCAACCTAA
CTGGAATGACTGTTCAACCCAATAAAGCAATTGTGGGAGCTAATGCTTTTGCCCATGAATCAGGCATTCATCAGGATGGA
GTCTTAAAAAATAGACTAACTTACGAAATTATCGATGCAAAAACTGTTGGTTTGAGTGATAACAAAATATCTTTGGGAAA
ACTTAGTGGAAGAAGTGCAGTAAGAGCTAGATTAGAGGAGATGGGATATGACTTGAGCAGGGAAGACTTAAATGATGCTT
TTGCTCGTTTTAAGGATTTAGCTGATAGGAAAAGAGAAATTACAGATAGAGACTTAGAAGCAATTGTTAGTGAACAAGTA
CAGCTTCCGGAAGCTAAATTTCAGTTATGTCTTGTACAAGTAAGTTGCGGTAACGCATCTAAACCTACCGCCACTATTAC
GCTTCTAAACACGGCAGATAATACTGAGGATACAGCTGTATCAGTAGGGACTGGACCTGTTGATGCTGTGTGCGAGGCTT
TAAATAAATTAGCTAAAGTTCCTAATGAATTAATTGAATTTTCTGTAAAGTCGGTAACAGAAGGTATTGATGCATTGGGA
GAAGTAACAATAAGAATCAGAAGGGATAATAAGATATATTCTGGTCATTCTGCTGACACGGATGTGGTAGTTGCTGCGGC
GAATGCTTACGTTAATGCTTTAAATAGGCTTGTATTTTCTGAGAAAAAAAATTCAATTCACCCACAATTTGATAATTTAG
AAAATTCTAATAAAACATTTCTATCTAATCCTGCAAATTAA

Upstream 100 bases:

>100_bases
CCAATATTGTTAAGTAATAAGCCCGCAGCAAATTTTTATTGAACTAGATCTAGTCAATTTTTTTTAATTAGCTTAATATA
AGTTTAAGTGATTCGCGCGA

Downstream 100 bases:

>100_bases
ATTATTTCTTAGGATTATTAAGTAGTATAAAAAATAGTCTCTTAATACTGTAGATTTATTTAATAGTTTAAGCTGTAAAT
AATGAGAGAAAGGTTACTAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKISNDVGKENGPIICGLA
RASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKSRKDVLQIVPEMVNYAKTLVEDIEFSCEDASRSDPDFLYEV
IQLAISAGATTINIPDTVGFTTPSEFGKLISDINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQV
QLPEAKFQLCLVQVSCGNASKPTATITLLNTADNTEDTAVSVGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
EVTIRIRRDNKIYSGHSADTDVVVAAANAYVNALNRLVFSEKKNSIHPQFDNLENSNKTFLSNPAN

Sequences:

>Translated_546_residues
MSKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKISNDVGKENGPIICGLA
RASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKSRKDVLQIVPEMVNYAKTLVEDIEFSCEDASRSDPDFLYEV
IQLAISAGATTINIPDTVGFTTPSEFGKLISDINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQV
QLPEAKFQLCLVQVSCGNASKPTATITLLNTADNTEDTAVSVGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
EVTIRIRRDNKIYSGHSADTDVVVAAANAYVNALNRLVFSEKKNSIHPQFDNLENSNKTFLSNPAN
>Mature_545_residues
SKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKISNDVGKENGPIICGLAR
ASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKSRKDVLQIVPEMVNYAKTLVEDIEFSCEDASRSDPDFLYEVI
QLAISAGATTINIPDTVGFTTPSEFGKLISDINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGER
AGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQPNKAIVGANAFAHESGIHQDGV
LKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQ
LPEAKFQLCLVQVSCGNASKPTATITLLNTADNTEDTAVSVGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALGE
VTIRIRRDNKIYSGHSADTDVVVAAANAYVNALNRLVFSEKKNSIHPQFDNLENSNKTFLSNPAN

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=513, Percent_Identity=46.3937621832359, Blast_Score=434, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320019, Length=387, Percent_Identity=29.1989664082687, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=389, Percent_Identity=28.5347043701799, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=554, Percent_Identity=25.2707581227437, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6324682, Length=394, Percent_Identity=26.3959390862944, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 59254; Mature: 59122

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKA
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VNKISNDVGKENGPIICGLARASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKS
HHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RKDVLQIVPEMVNYAKTLVEDIEFSCEDASRSDPDFLYEVIQLAISAGATTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
TTPSEFGKLISDINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHHHHHHHHHHCCCCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHC
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEECC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEE
CCEEEECCHHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
MGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPEAKFQLCLVQVSCGNAS
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEEEEEECCCCC
KPTATITLLNTADNTEDTAVSVGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
CCEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
EVTIRIRRDNKIYSGHSADTDVVVAAANAYVNALNRLVFSEKKNSIHPQFDNLENSNKTF
EEEEEEEECCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LSNPAN
CCCCCC
>Mature Secondary Structure 
SKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKA
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VNKISNDVGKENGPIICGLARASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKS
HHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RKDVLQIVPEMVNYAKTLVEDIEFSCEDASRSDPDFLYEVIQLAISAGATTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
TTPSEFGKLISDINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHHHHHHHHHHCCCCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHC
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEECC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEE
CCEEEECCHHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
MGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPEAKFQLCLVQVSCGNAS
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEEEEEECCCCC
KPTATITLLNTADNTEDTAVSVGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
CCEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
EVTIRIRRDNKIYSGHSADTDVVVAAANAYVNALNRLVFSEKKNSIHPQFDNLENSNKTF
EEEEEEEECCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LSNPAN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA