Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 78779448

GI number: 78779448

Start: 975558

End: 976031

Strand: Reverse

Name: ruvC

Synonym: PMT9312_1065

Alternate gene names: 78779448

Gene position: 976031-975558 (Counterclockwise)

Preceding gene: 78779449

Following gene: 78779447

Centisome position: 57.1

GC content: 34.18

Gene sequence:

>474_bases
GTGAGAATAATTGGGATAGACCCTGGATTAGCCAGAGTTGGATATGGAATTATTGAAATAAAAAATGATAAAAAGATATT
GCTAGATTGTGGAGTTATTGAGACAGGTAAAGAAAAAAAAGAAGAGGATAGACTTTATGAGATATTCAAAGATCTTAATG
AATTAATAAGTCATTGGAAGCCAAATTTAGCAGCGGTAGAGAAATTTTTCTTTTATAGATCTAGTACTACTATTAGTGTG
GTGCAGGCTAGAGGCGTGATTATGATGGTATTGGCCTCTAAAAAAATTAATATTAGTGAATATTCCCCTGCACAGATAAA
GTTAACCATTGCAGGGTCAGGAAAAGCATCTAAGAAAGAAGTTCTTGATGCGGTTATGTATAACTTGGAACTTAACAAAC
CTCCAAAACCTGATGATTCAGCTGATGCATTGGCCATAGCATTAACAAAACTAAATGAGGAAGGCTTTAACTGA

Upstream 100 bases:

>100_bases
ATTCAGGTGAAAAAGTTATTCAAGCTTTTTGTAAAGTATTTGAGTTAGATGAAAAAGATAATCTTTCTAAATTTCAATTG
TCTGCAGAAGCCTAATTACA

Downstream 100 bases:

>100_bases
GTATCTAATATGACTATCTTTGATAAAAAGAAATTTGAGAGGGATACGTTTAGAAAACTTAGAAATGAGATTTCTCTAAT
TCAAAGGGAAAATGTAGAAA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIIGIDPGLARVGYGIIEIKNDKKILLDCGVIETGKEKKEEDRLYEIFKDLNELISHWKPNLAAVEKFFFYRSSTTISV
VQARGVIMMVLASKKINISEYSPAQIKLTIAGSGKASKKEVLDAVMYNLELNKPPKPDDSADALAIALTKLNEEGFN

Sequences:

>Translated_157_residues
MRIIGIDPGLARVGYGIIEIKNDKKILLDCGVIETGKEKKEEDRLYEIFKDLNELISHWKPNLAAVEKFFFYRSSTTISV
VQARGVIMMVLASKKINISEYSPAQIKLTIAGSGKASKKEVLDAVMYNLELNKPPKPDDSADALAIALTKLNEEGFN
>Mature_157_residues
MRIIGIDPGLARVGYGIIEIKNDKKILLDCGVIETGKEKKEEDRLYEIFKDLNELISHWKPNLAAVEKFFFYRSSTTISV
VQARGVIMMVLASKKINISEYSPAQIKLTIAGSGKASKKEVLDAVMYNLELNKPPKPDDSADALAIALTKLNEEGFN

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=135, Percent_Identity=31.8518518518519, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 17460; Mature: 17460

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIGIDPGLARVGYGIIEIKNDKKILLDCGVIETGKEKKEEDRLYEIFKDLNELISHWK
CEEEECCCCHHHHCCEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PNLAAVEKFFFYRSSTTISVVQARGVIMMVLASKKINISEYSPAQIKLTIAGSGKASKKE
CCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHH
VLDAVMYNLELNKPPKPDDSADALAIALTKLNEEGFN
HHHHHHHHEECCCCCCCCCCCHHHHHHEEHHCCCCCC
>Mature Secondary Structure
MRIIGIDPGLARVGYGIIEIKNDKKILLDCGVIETGKEKKEEDRLYEIFKDLNELISHWK
CEEEECCCCHHHHCCEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PNLAAVEKFFFYRSSTTISVVQARGVIMMVLASKKINISEYSPAQIKLTIAGSGKASKKE
CCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHH
VLDAVMYNLELNKPPKPDDSADALAIALTKLNEEGFN
HHHHHHHHEECCCCCCCCCCCHHHHHHEEHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA