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The map label for this gene is purT

Identifier: 78779398

GI number: 78779398

Start: 936861

End: 938036

Strand: Reverse

Name: purT

Synonym: PMT9312_1014

Alternate gene names: 78779398

Gene position: 938036-936861 (Counterclockwise)

Preceding gene: 78779399

Following gene: 78779397

Centisome position: 54.88

GC content: 31.12

Gene sequence:

>1176_bases
ATGAATGATTCAGTTTTTTCTAAAAAAAGAATTTTATTACTTGGTAGTGGCGAGCTTGGGAAGGAATTAGTAATAGAATC
CAAAAGATTAGGATTAGAAGTTATTGCTATTGATCGATATGAGAAAGCACCGGCAATGCAAGTCGCTGATTATTCAAAAG
TAATTGATATGGGAGATAAAAATATTTTAAAAAATTCTATAAAAGAATTTACTCCCGACTTTGTTGTCCCTGAAATAGAG
GCACTTTCAATTGAAGCCTTAAAAGAATTAGAGGATGAAGGTTTCAAGATTGTCCCAAATGCTAGAACTGTAGAAATAAC
AATGAATAGAGATAAAATTAGAGAATTGGTTTCTAGAGATTTAAAAATAAAAACTGCAAATTATGATTATATTTATAAAT
TTGAGGACCTAGAAAAAAAAGCAGATGAAATTGGATTTCCACTTTTACTTAAGCCTTTAATGAGCTCTTCAGGTAAGGGA
CAAAGTTTGGTTGGATCAAAAAATGATTTAAATCAAGCCTGGAGTCAGGCACAAGCAAATTCTAGAGGCCAAGTTAAAGG
GGTAATTATTGAAGAATTTATTAATTTTGATTTTGAGTTTACTCTTTTAACTGTAAGAAAAAACAACGGTGAAAATATTT
TTTGTTTACCAATCGGACATCTTCAATCTAATGGAGATTATCAATGCAGTTGGCATCCTTTGGAGATTAATCAATCCTTA
ATTAGTGAAGCCAAGAAAATGACAACTAAAATTTTAAATAATCTAAATGGATCTGGATTATACGGTGTAGAGTTTTTTGT
TAAAGAAAATGAGGTTATATTTTCAGAATTATCTCCACGACCTCATGACACTGGTATGGTTACATTAGTTAGTCAAAATA
TTAATGAATTTGAATTACATTTAAGGGCTTTTTTAAATTTACCTATACCCCATATAAATCTAATAGAACCCTCTGCAACC
AGAGTTATACTTTCCGACCAAGAGCATATGAATCCTATTTATGAAGGTCTTAATGAAGCATTAGAAGTTGAAAATACTAA
AGTACTCATATTTGGCAAGCCAATCTCCAGGAAAGGCAGAAGAATGGGAGTTGTTCTTTCATCAAATTCAGACATTAATT
TGGCTAGGAAAAATGCTGATGAAGCTGCTCGTAAAATAAAAGTCAGCTCTAAATAA

Upstream 100 bases:

>100_bases
GTTGGGTACGAAATCTTTATTTAGTTTCTCTAAAGATAAAGAAAATAGAGATGATTCAACTAGTCAGCTTTCTGATGATG
ATGAATAATACTTTATAAAT

Downstream 100 bases:

>100_bases
ATATTAAGAAAACATAACGAAAAAAATACTTATTTCCTTTGTTTTTGTTTATTCTTTCTCTGAGTATTATCAATAATAGA
TCTTTTAATCTAAATGATAG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MNDSVFSKKRILLLGSGELGKELVIESKRLGLEVIAIDRYEKAPAMQVADYSKVIDMGDKNILKNSIKEFTPDFVVPEIE
ALSIEALKELEDEGFKIVPNARTVEITMNRDKIRELVSRDLKIKTANYDYIYKFEDLEKKADEIGFPLLLKPLMSSSGKG
QSLVGSKNDLNQAWSQAQANSRGQVKGVIIEEFINFDFEFTLLTVRKNNGENIFCLPIGHLQSNGDYQCSWHPLEINQSL
ISEAKKMTTKILNNLNGSGLYGVEFFVKENEVIFSELSPRPHDTGMVTLVSQNINEFELHLRAFLNLPIPHINLIEPSAT
RVILSDQEHMNPIYEGLNEALEVENTKVLIFGKPISRKGRRMGVVLSSNSDINLARKNADEAARKIKVSSK

Sequences:

>Translated_391_residues
MNDSVFSKKRILLLGSGELGKELVIESKRLGLEVIAIDRYEKAPAMQVADYSKVIDMGDKNILKNSIKEFTPDFVVPEIE
ALSIEALKELEDEGFKIVPNARTVEITMNRDKIRELVSRDLKIKTANYDYIYKFEDLEKKADEIGFPLLLKPLMSSSGKG
QSLVGSKNDLNQAWSQAQANSRGQVKGVIIEEFINFDFEFTLLTVRKNNGENIFCLPIGHLQSNGDYQCSWHPLEINQSL
ISEAKKMTTKILNNLNGSGLYGVEFFVKENEVIFSELSPRPHDTGMVTLVSQNINEFELHLRAFLNLPIPHINLIEPSAT
RVILSDQEHMNPIYEGLNEALEVENTKVLIFGKPISRKGRRMGVVLSSNSDINLARKNADEAARKIKVSSK
>Mature_391_residues
MNDSVFSKKRILLLGSGELGKELVIESKRLGLEVIAIDRYEKAPAMQVADYSKVIDMGDKNILKNSIKEFTPDFVVPEIE
ALSIEALKELEDEGFKIVPNARTVEITMNRDKIRELVSRDLKIKTANYDYIYKFEDLEKKADEIGFPLLLKPLMSSSGKG
QSLVGSKNDLNQAWSQAQANSRGQVKGVIIEEFINFDFEFTLLTVRKNNGENIFCLPIGHLQSNGDYQCSWHPLEINQSL
ISEAKKMTTKILNNLNGSGLYGVEFFVKENEVIFSELSPRPHDTGMVTLVSQNINEFELHLRAFLNLPIPHINLIEPSAT
RVILSDQEHMNPIYEGLNEALEVENTKVLIFGKPISRKGRRMGVVLSSNSDINLARKNADEAARKIKVSSK

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=382, Percent_Identity=44.7643979057592, Blast_Score=351, Evalue=6e-98,
Organism=Escherichia coli, GI1786733, Length=314, Percent_Identity=24.5222929936306, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=24.5901639344262, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_PROM9 (Q31AM1)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397510.1
- ProteinModelPortal:   Q31AM1
- SMR:   Q31AM1
- STRING:   Q31AM1
- GeneID:   3765818
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1014
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- ProtClustDB:   PRK09288
- BioCyc:   PMAR74546:PMT9312_1014-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 44028; Mature: 44028

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 78-78 BINDING 110-110 BINDING 151-151 BINDING 199-199 BINDING 264-264 BINDING 276-276 BINDING 283-283 BINDING 353-353

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSVFSKKRILLLGSGELGKELVIESKRLGLEVIAIDRYEKAPAMQVADYSKVIDMGDK
CCCCCCCCCEEEEEECCCCCHHHHEEHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCH
NILKNSIKEFTPDFVVPEIEALSIEALKELEDEGFKIVPNARTVEITMNRDKIRELVSRD
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCEEEEEEECHHHHHHHHCCC
LKIKTANYDYIYKFEDLEKKADEIGFPLLLKPLMSSSGKGQSLVGSKNDLNQAWSQAQAN
CEEEECCCEEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
SRGQVKGVIIEEFINFDFEFTLLTVRKNNGENIFCLPIGHLQSNGDYQCSWHPLEINQSL
CCCCEEEEEEHHHHCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCEEEEEEEHCCCHHH
ISEAKKMTTKILNNLNGSGLYGVEFFVKENEVIFSELSPRPHDTGMVTLVSQNINEFELH
HHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEECCCCHHHHH
LRAFLNLPIPHINLIEPSATRVILSDQEHMNPIYEGLNEALEVENTKVLIFGKPISRKGR
HHHHHCCCCCCEEEECCCCCEEEECCHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCC
RMGVVLSSNSDINLARKNADEAARKIKVSSK
EEEEEEECCCCCEEECCCHHHHHHEEECCCC
>Mature Secondary Structure
MNDSVFSKKRILLLGSGELGKELVIESKRLGLEVIAIDRYEKAPAMQVADYSKVIDMGDK
CCCCCCCCCEEEEEECCCCCHHHHEEHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCH
NILKNSIKEFTPDFVVPEIEALSIEALKELEDEGFKIVPNARTVEITMNRDKIRELVSRD
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCEEEEEEECHHHHHHHHCCC
LKIKTANYDYIYKFEDLEKKADEIGFPLLLKPLMSSSGKGQSLVGSKNDLNQAWSQAQAN
CEEEECCCEEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
SRGQVKGVIIEEFINFDFEFTLLTVRKNNGENIFCLPIGHLQSNGDYQCSWHPLEINQSL
CCCCEEEEEEHHHHCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCEEEEEEEHCCCHHH
ISEAKKMTTKILNNLNGSGLYGVEFFVKENEVIFSELSPRPHDTGMVTLVSQNINEFELH
HHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEECCCCHHHHH
LRAFLNLPIPHINLIEPSATRVILSDQEHMNPIYEGLNEALEVENTKVLIFGKPISRKGR
HHHHHCCCCCCEEEECCCCCEEEECCHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCC
RMGVVLSSNSDINLARKNADEAARKIKVSSK
EEEEEEECCCCCEEECCCHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA