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The map label for this gene is metG [H]

Identifier: 78779318

GI number: 78779318

Start: 869744

End: 871279

Strand: Direct

Name: metG [H]

Synonym: PMT9312_0934

Alternate gene names: 78779318

Gene position: 869744-871279 (Clockwise)

Preceding gene: 78779317

Following gene: 78779319

Centisome position: 50.89

GC content: 29.69

Gene sequence:

>1536_bases
ATGACTTTTGTCATTACTACACCTTTATACTATGTTAATGACAAACCTCATTTAGGAAGTGTATATACAACAATAATTTG
TGACTCGATAGCTAGGTATAAAAGACTTGTAGGTGAAGATGTTATTTTCATTACTGGTGTTGATGAACATGGTTTAAAAA
TACAAAGAACAGCTAATGAAAAGGGTATTGAACCAAAATCACATTGTGATGAAATCTCAGAAGTATTTAATATTAATTGG
AAAAATTGGAATATATCCTTTGATAAATTTATAAGGACAAGCTCAAAAAATCATGAATTTGTTGTTAATGAATTTTATGA
AAGAGTAAAATCATCAGATGATATCTATATGGGAGTTCAAAAAGGTTGGTATTGTGTCGGTTGTGAAGAATTTAAAGATA
ATCCAGAAAACTCATCAACATACAAGTGTCCCATACATCAAAAAAATTTAGAATGGAAAAATGAAGAAAATCTCTTTTTT
AGGCTTTCGAAATATCAAAAAGAAATTGAAAAAATAATCAACGAACCTTCTTTTATAGAGCCAATAGAAAGAAAGAATGA
AATTATAAATTTTGTTTCTAGAGGTTTAAAGGATTTTTCAATTTCAAGAACAAATGTCTCATGGGGAATTCCTGTCCCTG
GTTACGATAACCATACGTTTTATGTATGGTTTGATGCTTTACTTGGATATGTAAGTGCCATTAGTTCTGATACATCAGAT
AATTCATTGGAAAGATCAATTAATGGAGGATGGCCAGCTGATGTTCATTTAATTGGTAAGGATATACTGAGATTCCATGC
TGTATATTGGCCTGCAATGCTCATTTCTGCGAATATGAAAGTCCCTAAAAAAGTTTTCGGGCATGGGTTTCTTACAAGAG
AGGGGCAAAAAATGGGTAAAAGCTTGGGAAATGTACTCGACCCTGATTTATTGCTTTCTAAATATGGAAATGATCCTGTG
AGGTGGTACCTCATTAAAGATATATCACTAGGTAATGATGGAGATTTTCAACATAAAAGATTTGTTGACATTATCAATAA
TGACTTAGCCAATACAATTGGTAATTTACTAAATAGAACATCATCTATGTCTAGAAAATGGTTTGGCAATAAAGTGCCAA
ATAATGAAAATATTATAAATGAAAATAAATTAGAGAATTATGCCAAAATTTCAGTTAAAAACTATATACATAACTTTGAT
ATTTACAAATTAGATCTAGCAGCTAATGAAGTGCTTAGCCTAGCCATAAATACAAATTTGTATTTGAATGATAATCAACC
ATGGATGTTAATAAAAGAGATTGATAATTTACCTCTTGTTAAAGAAATTATTTACAATGTATTAGAAAGTACCCGAATAA
TAGGATTATTATTAATACCTATATTACCCGAATTATCAACAAAAATTAACGAGCAACTTGGTTCAATATACAGAAAAGAA
ATTCCTTGGAAACAACAATTAAAATGGGGATTATTAGTTAGTAACTCAAGCCTTCCTAAACCCTCACCAATAATAAATAA
GCTTGAGTATGAATAA

Upstream 100 bases:

>100_bases
ATGATAGTAATCTTTTAGAATTTATTTATTAATAATATAATAAATATAATAGGTTGTTATTAGTTTAAAAAATATCTGTT
AGTATTAATAAAAAAGCTTT

Downstream 100 bases:

>100_bases
GATATATAGATTATTAATTTTCCTTCCTTTTTTTATGCTTGGTTGCGTACCAAATATCATTGAAGAAAATAAAGTAATAC
AAAAAATAAATACTTTAGAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLVGEDVIFITGVDEHGLKIQRTANEKGIEPKSHCDEISEVFNINW
KNWNISFDKFIRTSSKNHEFVVNEFYERVKSSDDIYMGVQKGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFF
RLSKYQKEIEKIINEPSFIEPIERKNEIINFVSRGLKDFSISRTNVSWGIPVPGYDNHTFYVWFDALLGYVSAISSDTSD
NSLERSINGGWPADVHLIGKDILRFHAVYWPAMLISANMKVPKKVFGHGFLTREGQKMGKSLGNVLDPDLLLSKYGNDPV
RWYLIKDISLGNDGDFQHKRFVDIINNDLANTIGNLLNRTSSMSRKWFGNKVPNNENIINENKLENYAKISVKNYIHNFD
IYKLDLAANEVLSLAINTNLYLNDNQPWMLIKEIDNLPLVKEIIYNVLESTRIIGLLLIPILPELSTKINEQLGSIYRKE
IPWKQQLKWGLLVSNSSLPKPSPIINKLEYE

Sequences:

>Translated_511_residues
MTFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLVGEDVIFITGVDEHGLKIQRTANEKGIEPKSHCDEISEVFNINW
KNWNISFDKFIRTSSKNHEFVVNEFYERVKSSDDIYMGVQKGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFF
RLSKYQKEIEKIINEPSFIEPIERKNEIINFVSRGLKDFSISRTNVSWGIPVPGYDNHTFYVWFDALLGYVSAISSDTSD
NSLERSINGGWPADVHLIGKDILRFHAVYWPAMLISANMKVPKKVFGHGFLTREGQKMGKSLGNVLDPDLLLSKYGNDPV
RWYLIKDISLGNDGDFQHKRFVDIINNDLANTIGNLLNRTSSMSRKWFGNKVPNNENIINENKLENYAKISVKNYIHNFD
IYKLDLAANEVLSLAINTNLYLNDNQPWMLIKEIDNLPLVKEIIYNVLESTRIIGLLLIPILPELSTKINEQLGSIYRKE
IPWKQQLKWGLLVSNSSLPKPSPIINKLEYE
>Mature_510_residues
TFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLVGEDVIFITGVDEHGLKIQRTANEKGIEPKSHCDEISEVFNINWK
NWNISFDKFIRTSSKNHEFVVNEFYERVKSSDDIYMGVQKGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFFR
LSKYQKEIEKIINEPSFIEPIERKNEIINFVSRGLKDFSISRTNVSWGIPVPGYDNHTFYVWFDALLGYVSAISSDTSDN
SLERSINGGWPADVHLIGKDILRFHAVYWPAMLISANMKVPKKVFGHGFLTREGQKMGKSLGNVLDPDLLLSKYGNDPVR
WYLIKDISLGNDGDFQHKRFVDIINNDLANTIGNLLNRTSSMSRKWFGNKVPNNENIINENKLENYAKISVKNYIHNFDI
YKLDLAANEVLSLAINTNLYLNDNQPWMLIKEIDNLPLVKEIIYNVLESTRIIGLLLIPILPELSTKINEQLGSIYRKEI
PWKQQLKWGLLVSNSSLPKPSPIINKLEYE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=503, Percent_Identity=34.7912524850895, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI14043022, Length=520, Percent_Identity=26.3461538461538, Blast_Score=159, Evalue=5e-39,
Organism=Escherichia coli, GI1788432, Length=514, Percent_Identity=25.6809338521401, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI212645096, Length=503, Percent_Identity=31.2127236580517, Blast_Score=228, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI17540794, Length=558, Percent_Identity=26.3440860215054, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI71992781, Length=343, Percent_Identity=31.1953352769679, Blast_Score=156, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321610, Length=510, Percent_Identity=33.5294117647059, Blast_Score=253, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6321704, Length=522, Percent_Identity=24.3295019157088, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21358483, Length=490, Percent_Identity=31.2244897959184, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24655311, Length=547, Percent_Identity=26.3254113345521, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 59052; Mature: 58921

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLVGEDVIFITGVDEHGLKIQRTANE
CEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCC
KGIEPKSHCDEISEVFNINWKNWNISFDKFIRTSSKNHEFVVNEFYERVKSSDDIYMGVQ
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEC
KGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFFRLSKYQKEIEKIINEPSFIE
CCEEEEECHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCC
PIERKNEIINFVSRGLKDFSISRTNVSWGIPVPGYDNHTFYVWFDALLGYVSAISSDTSD
HHHHHHHHHHHHHCCCHHCEEEECCCEECCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCC
NSLERSINGGWPADVHLIGKDILRFHAVYWPAMLISANMKVPKKVFGHGFLTREGQKMGK
CHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHH
SLGNVLDPDLLLSKYGNDPVRWYLIKDISLGNDGDFQHKRFVDIINNDLANTIGNLLNRT
HHHHHCCHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SSMSRKWFGNKVPNNENIINENKLENYAKISVKNYIHNFDIYKLDLAANEVLSLAINTNL
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECEE
YLNDNQPWMLIKEIDNLPLVKEIIYNVLESTRIIGLLLIPILPELSTKINEQLGSIYRKE
EECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IPWKQQLKWGLLVSNSSLPKPSPIINKLEYE
CCHHHCCCEEEEEECCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLVGEDVIFITGVDEHGLKIQRTANE
EEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCC
KGIEPKSHCDEISEVFNINWKNWNISFDKFIRTSSKNHEFVVNEFYERVKSSDDIYMGVQ
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEC
KGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFFRLSKYQKEIEKIINEPSFIE
CCEEEEECHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCC
PIERKNEIINFVSRGLKDFSISRTNVSWGIPVPGYDNHTFYVWFDALLGYVSAISSDTSD
HHHHHHHHHHHHHCCCHHCEEEECCCEECCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCC
NSLERSINGGWPADVHLIGKDILRFHAVYWPAMLISANMKVPKKVFGHGFLTREGQKMGK
CHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHH
SLGNVLDPDLLLSKYGNDPVRWYLIKDISLGNDGDFQHKRFVDIINNDLANTIGNLLNRT
HHHHHCCHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SSMSRKWFGNKVPNNENIINENKLENYAKISVKNYIHNFDIYKLDLAANEVLSLAINTNL
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECEE
YLNDNQPWMLIKEIDNLPLVKEIIYNVLESTRIIGLLLIPILPELSTKINEQLGSIYRKE
EECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IPWKQQLKWGLLVSNSSLPKPSPIINKLEYE
CCHHHCCCEEEEEECCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]