Click here to switch to the map view.

The map label for this gene is pheT

Identifier: 78779314

GI number: 78779314

Start: 865472

End: 867916

Strand: Reverse

Name: pheT

Synonym: PMT9312_0930

Alternate gene names: 78779314

Gene position: 867916-865472 (Counterclockwise)

Preceding gene: 78779320

Following gene: 78779313

Centisome position: 50.78

GC content: 30.55

Gene sequence:

>2445_bases
ATGAAAGTTTCTCAAAATTGGTTGAAAAATTTAGTAGAAATTTCTTCTACTCCTGTAGATCTCTCTGAGAAATTATCTAT
TGGTGGATTTGAGGTTGAATCATTAGAAGATTGTTCTAAAAATGTAAATGGTGTTGTTTTAGGTAAGGTCTTATCTGTTT
TAAAACACGATGGATCTGACAAACTCTCAATTTGCCAAGTCGATATTGGTACTTCAAAGAATCTACAAATTATCTGTGGT
GCGCGAAATATTAAACCAAATGTTTATGTTTATGTTGCTACTGTCGGCGCCAAATTAAATGCCGTTGATTTAACTATTAA
AAGAAGTGAAATTAGAGGTGTCATGAGTGAAGGAATGATATGCTCTCTGCAGGAACTAGGATTAGAAGACTCTAGCGAAG
GGATAGAGATCATTGATGAAGATTTAGCCTTAAAACATGAATTAGGCACTCCAGTGTCTGACTTACTTCAATTAAATGAT
TTTATATATGATTTAGCCATTACAGCTAATAGACCTGACGGAATGTCGGTTATAGGTATAGCCCGAGAAATTTCTGCCCT
TTTAGAATCCAAATTAAGTTTTCCAGAATTAAACCATAAATACAATATTCATTTAAATAAGGGAATTAAACTTTGTCCAG
AGGCTATAACATCTAATTGCATTTATACAATAAGCTGCATTGATGGAGTAAATGGAAATAAATTATCACCAATATGGCTT
AAAGACCGTATAGAAAAATCAGGTATAAAGTCGATTAATCTTCTAGTTGATTTAACAAACTATATACTTTTAGAACAAGG
GCAACCCTTGCATGCGTTTGACAAGGAAAAATTATCTAACTTAATTGGCAAGGATGTTTCTCCAGAAGACTTCTCTGTAC
GAAAAGCCAAGGATAACGAAAGCCTTGTATGTTTGGATGGAAAAAAATATGAATTAAATGAAAATATTACAGTAGTTACT
TGTTCCGATAAACCTGTTGCTATTGCTGGGGTGATAGGTGGTTTAGAGACTTCTGTAAGCAATACTACCTCTTCTATTTA
CCTTGAGGGAGCTGTTTTTAATCCAGTTACCATAAGAAAATCTTCAAAGGCGGTTGGCATAAGAACAGAATCTAGCAGCA
GATATGAAAAAGGGATTTCGTCAAAAAACACAATAAGCGCTGTTACAAGAGCAATGAATCTTTTAGAAGAATATTTTTCT
ATTAATTTACCCATCATCAATACTTCTAATTTAAAAAATAATGAGGATATTTTTATTAAATTACGGAGAAGTCGAATACA
TAAAATTCTTGGTCCATTAATCATTAACGATCAATTTGAAAAAAGAAATATATCTGATACTGAAATAGTTGATAAATTAA
CACTCATAGGTTGTTCTTTAAAAAGTAAAGAATATGGTTGGGATGTAGCAGTAATTCCAAATAGATCACAAGATTTAATA
AGAGAAATAGATTTAATTGAAGAAATTGCGAGATTAATAGGGTATGACAGATTCGACTTGAACCTTCCTAATCCAATTAA
ACCTGGGAAATTGTCATCAGAGCAGTTGGCATTGAGAAAGGTAAAAAATGGATTTACAGAAAATGGTTTTAACGAGGTTC
TTAGCTACTCTCTTGTTCCAGAAGATAAAGAAACACTTATAAAGATATCTAATCCTTTGTTATTAGAAACTAGCTGCCTA
AGAGATAATATTTGGAAAGAACATTTGGAAATAGTAAACCGTAATATAAAAGCTGGACAAAACAGTTGTTATATATTTGA
AATTGGAAATGTTTTTCATAAGAATACTGAGTTCATTCAAAAAGAAGTACTGAATGGTGCAATTTTTGGCAATAAAAGAT
TTGAAAAGTGGGTAAATTCTAACAAGGATAATGATCTTAATTATTATCAGGCTAGAGGAAAGTTAAAGGAAGCCTTATCA
TCTTTGAATATAAAAATTGATGATAAACCTACTGATTCAATTGATTTTCTACATCCAGGCAGAACAGCAAAATTGATTAT
TGAAGGAAAAGATGCAGGTTATTTTGGCGAAATACATCCCAAATTAATACTAGAAAAGAAATCATTAAAAATCGTTTATT
TATTTAGCATAAATGTAGATAACCTTTTGGGTGCTAGTACAAGAAAAAATAAATGGATTCCAATATTTAAGCAATACCCA
ATTGTTCCGAAAATAGAAAGGGATATAAATTTTGTTTTCAGTAAGAAATTCTTAATTAGCGATATAACTTCACTAATAAA
AAAAACAGGAAAAAATCTTTTAGAGGATGTTTATTTAATAGATATTTTTGAAGATATTAAACTTGGGGAGGATTATATAA
GCTATACATTTAGATTATCTTATAGAGCTCAAGATAAAACATTACTGGACTCAGATATCAAAACAATACACTCAAGCATC
ATTTCTAATATTGAAAAAAGTTTTCAGACTAAATTGAGAAATTAG

Upstream 100 bases:

>100_bases
TTTAATTAATACTTTATTGTATAATTTTCTATTGTACATCTTCAACAAACTATTTTGAAGATATAATCATTTCTTTTATC
ATCATTGATTAAAAATATAT

Downstream 100 bases:

>100_bases
CTTCCCAAAAGTCTTATATTAGACTAAAAACTAGTCTAATATTAATTCTAGTATAAGACAAGTAATAATCATATAAAACT
GATTAATGTTGTATTATAAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MKVSQNWLKNLVEISSTPVDLSEKLSIGGFEVESLEDCSKNVNGVVLGKVLSVLKHDGSDKLSICQVDIGTSKNLQIICG
ARNIKPNVYVYVATVGAKLNAVDLTIKRSEIRGVMSEGMICSLQELGLEDSSEGIEIIDEDLALKHELGTPVSDLLQLND
FIYDLAITANRPDGMSVIGIAREISALLESKLSFPELNHKYNIHLNKGIKLCPEAITSNCIYTISCIDGVNGNKLSPIWL
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKEKLSNLIGKDVSPEDFSVRKAKDNESLVCLDGKKYELNENITVVT
CSDKPVAIAGVIGGLETSVSNTTSSIYLEGAVFNPVTIRKSSKAVGIRTESSSRYEKGISSKNTISAVTRAMNLLEEYFS
INLPIINTSNLKNNEDIFIKLRRSRIHKILGPLIINDQFEKRNISDTEIVDKLTLIGCSLKSKEYGWDVAVIPNRSQDLI
REIDLIEEIARLIGYDRFDLNLPNPIKPGKLSSEQLALRKVKNGFTENGFNEVLSYSLVPEDKETLIKISNPLLLETSCL
RDNIWKEHLEIVNRNIKAGQNSCYIFEIGNVFHKNTEFIQKEVLNGAIFGNKRFEKWVNSNKDNDLNYYQARGKLKEALS
SLNIKIDDKPTDSIDFLHPGRTAKLIIEGKDAGYFGEIHPKLILEKKSLKIVYLFSINVDNLLGASTRKNKWIPIFKQYP
IVPKIERDINFVFSKKFLISDITSLIKKTGKNLLEDVYLIDIFEDIKLGEDYISYTFRLSYRAQDKTLLDSDIKTIHSSI
ISNIEKSFQTKLRN

Sequences:

>Translated_814_residues
MKVSQNWLKNLVEISSTPVDLSEKLSIGGFEVESLEDCSKNVNGVVLGKVLSVLKHDGSDKLSICQVDIGTSKNLQIICG
ARNIKPNVYVYVATVGAKLNAVDLTIKRSEIRGVMSEGMICSLQELGLEDSSEGIEIIDEDLALKHELGTPVSDLLQLND
FIYDLAITANRPDGMSVIGIAREISALLESKLSFPELNHKYNIHLNKGIKLCPEAITSNCIYTISCIDGVNGNKLSPIWL
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKEKLSNLIGKDVSPEDFSVRKAKDNESLVCLDGKKYELNENITVVT
CSDKPVAIAGVIGGLETSVSNTTSSIYLEGAVFNPVTIRKSSKAVGIRTESSSRYEKGISSKNTISAVTRAMNLLEEYFS
INLPIINTSNLKNNEDIFIKLRRSRIHKILGPLIINDQFEKRNISDTEIVDKLTLIGCSLKSKEYGWDVAVIPNRSQDLI
REIDLIEEIARLIGYDRFDLNLPNPIKPGKLSSEQLALRKVKNGFTENGFNEVLSYSLVPEDKETLIKISNPLLLETSCL
RDNIWKEHLEIVNRNIKAGQNSCYIFEIGNVFHKNTEFIQKEVLNGAIFGNKRFEKWVNSNKDNDLNYYQARGKLKEALS
SLNIKIDDKPTDSIDFLHPGRTAKLIIEGKDAGYFGEIHPKLILEKKSLKIVYLFSINVDNLLGASTRKNKWIPIFKQYP
IVPKIERDINFVFSKKFLISDITSLIKKTGKNLLEDVYLIDIFEDIKLGEDYISYTFRLSYRAQDKTLLDSDIKTIHSSI
ISNIEKSFQTKLRN
>Mature_814_residues
MKVSQNWLKNLVEISSTPVDLSEKLSIGGFEVESLEDCSKNVNGVVLGKVLSVLKHDGSDKLSICQVDIGTSKNLQIICG
ARNIKPNVYVYVATVGAKLNAVDLTIKRSEIRGVMSEGMICSLQELGLEDSSEGIEIIDEDLALKHELGTPVSDLLQLND
FIYDLAITANRPDGMSVIGIAREISALLESKLSFPELNHKYNIHLNKGIKLCPEAITSNCIYTISCIDGVNGNKLSPIWL
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKEKLSNLIGKDVSPEDFSVRKAKDNESLVCLDGKKYELNENITVVT
CSDKPVAIAGVIGGLETSVSNTTSSIYLEGAVFNPVTIRKSSKAVGIRTESSSRYEKGISSKNTISAVTRAMNLLEEYFS
INLPIINTSNLKNNEDIFIKLRRSRIHKILGPLIINDQFEKRNISDTEIVDKLTLIGCSLKSKEYGWDVAVIPNRSQDLI
REIDLIEEIARLIGYDRFDLNLPNPIKPGKLSSEQLALRKVKNGFTENGFNEVLSYSLVPEDKETLIKISNPLLLETSCL
RDNIWKEHLEIVNRNIKAGQNSCYIFEIGNVFHKNTEFIQKEVLNGAIFGNKRFEKWVNSNKDNDLNYYQARGKLKEALS
SLNIKIDDKPTDSIDFLHPGRTAKLIIEGKDAGYFGEIHPKLILEKKSLKIVYLFSINVDNLLGASTRKNKWIPIFKQYP
IVPKIERDINFVFSKKFLISDITSLIKKTGKNLLEDVYLIDIFEDIKLGEDYISYTFRLSYRAQDKTLLDSDIKTIHSSI
ISNIEKSFQTKLRN

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788006, Length=842, Percent_Identity=30.2850356294537, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_PROM9 (Q31AV5)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397426.1
- ProteinModelPortal:   Q31AV5
- STRING:   Q31AV5
- GeneID:   3765732
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0930
- eggNOG:   COG0072
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- ProtClustDB:   PRK00629
- BioCyc:   PMAR74546:PMT9312_0930-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 91364; Mature: 91364

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSQNWLKNLVEISSTPVDLSEKLSIGGFEVESLEDCSKNVNGVVLGKVLSVLKHDGSD
CCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEHHHHHHHHCCCCEEEHHHHHHHHHCCCCC
KLSICQVDIGTSKNLQIICGARNIKPNVYVYVATVGAKLNAVDLTIKRSEIRGVMSEGMI
CEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEEECCEEEEEEEEEEHHHHHHHHHCCCE
CSLQELGLEDSSEGIEIIDEDLALKHELGTPVSDLLQLNDFIYDLAITANRPDGMSVIGI
EEHHHCCCCCCCCCCEEECHHHHHHHHCCCCHHHHHHHHHHHEEEEEECCCCCCCEEHHH
AREISALLESKLSFPELNHKYNIHLNKGIKLCPEAITSNCIYTISCIDGVNGNKLSPIWL
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHCCCCEEEEEEECCCCCCCCCCCHH
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKEKLSNLIGKDVSPEDFSVRKAKDNE
HHHHHHCCCCEEEEEEEEHHEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCC
SLVCLDGKKYELNENITVVTCSDKPVAIAGVIGGLETSVSNTTSSIYLEGAVFNPVTIRK
CEEEECCCEEEECCCEEEEEECCCCEEEEEECCCCHHCCCCCCCEEEEECEEECCEEEEC
SSKAVGIRTESSSRYEKGISSKNTISAVTRAMNLLEEYFSINLPIINTSNLKNNEDIFIK
CCCEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEE
LRRSRIHKILGPLIINDQFEKRNISDTEIVDKLTLIGCSLKSKEYGWDVAVIPNRSQDLI
EHHHHHHHHHCCCEECCCHHCCCCCHHHHHHHHHHEECCCCCCCCCCEEEEECCCCHHHH
REIDLIEEIARLIGYDRFDLNLPNPIKPGKLSSEQLALRKVKNGFTENGFNEVLSYSLVP
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHEECCCC
EDKETLIKISNPLLLETSCLRDNIWKEHLEIVNRNIKAGQNSCYIFEIGNVFHKNTEFIQ
CCCHHEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHCCHHHHH
KEVLNGAIFGNKRFEKWVNSNKDNDLNYYQARGKLKEALSSLNIKIDDKPTDSIDFLHPG
HHHHCCEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCC
RTAKLIIEGKDAGYFGEIHPKLILEKKSLKIVYLFSINVDNLLGASTRKNKWIPIFKQYP
CEEEEEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECHHHHCCCCCCCCCCCCHHHCCC
IVPKIERDINFVFSKKFLISDITSLIKKTGKNLLEDVYLIDIFEDIKLGEDYISYTFRLS
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCEEEEEEEE
YRAQDKTLLDSDIKTIHSSIISNIEKSFQTKLRN
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVSQNWLKNLVEISSTPVDLSEKLSIGGFEVESLEDCSKNVNGVVLGKVLSVLKHDGSD
CCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEHHHHHHHHCCCCEEEHHHHHHHHHCCCCC
KLSICQVDIGTSKNLQIICGARNIKPNVYVYVATVGAKLNAVDLTIKRSEIRGVMSEGMI
CEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEEECCEEEEEEEEEEHHHHHHHHHCCCE
CSLQELGLEDSSEGIEIIDEDLALKHELGTPVSDLLQLNDFIYDLAITANRPDGMSVIGI
EEHHHCCCCCCCCCCEEECHHHHHHHHCCCCHHHHHHHHHHHEEEEEECCCCCCCEEHHH
AREISALLESKLSFPELNHKYNIHLNKGIKLCPEAITSNCIYTISCIDGVNGNKLSPIWL
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHCCCCEEEEEEECCCCCCCCCCCHH
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKEKLSNLIGKDVSPEDFSVRKAKDNE
HHHHHHCCCCEEEEEEEEHHEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCC
SLVCLDGKKYELNENITVVTCSDKPVAIAGVIGGLETSVSNTTSSIYLEGAVFNPVTIRK
CEEEECCCEEEECCCEEEEEECCCCEEEEEECCCCHHCCCCCCCEEEEECEEECCEEEEC
SSKAVGIRTESSSRYEKGISSKNTISAVTRAMNLLEEYFSINLPIINTSNLKNNEDIFIK
CCCEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEE
LRRSRIHKILGPLIINDQFEKRNISDTEIVDKLTLIGCSLKSKEYGWDVAVIPNRSQDLI
EHHHHHHHHHCCCEECCCHHCCCCCHHHHHHHHHHEECCCCCCCCCCEEEEECCCCHHHH
REIDLIEEIARLIGYDRFDLNLPNPIKPGKLSSEQLALRKVKNGFTENGFNEVLSYSLVP
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHEECCCC
EDKETLIKISNPLLLETSCLRDNIWKEHLEIVNRNIKAGQNSCYIFEIGNVFHKNTEFIQ
CCCHHEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHCCHHHHH
KEVLNGAIFGNKRFEKWVNSNKDNDLNYYQARGKLKEALSSLNIKIDDKPTDSIDFLHPG
HHHHCCEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCC
RTAKLIIEGKDAGYFGEIHPKLILEKKSLKIVYLFSINVDNLLGASTRKNKWIPIFKQYP
CEEEEEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECHHHHCCCCCCCCCCCCHHHCCC
IVPKIERDINFVFSKKFLISDITSLIKKTGKNLLEDVYLIDIFEDIKLGEDYISYTFRLS
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCEEEEEEEE
YRAQDKTLLDSDIKTIHSSIISNIEKSFQTKLRN
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA