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The map label for this gene is yjfH [C]

Identifier: 78779235

GI number: 78779235

Start: 786272

End: 787282

Strand: Direct

Name: yjfH [C]

Synonym: PMT9312_0851

Alternate gene names: 78779235

Gene position: 786272-787282 (Clockwise)

Preceding gene: 78779234

Following gene: 78779236

Centisome position: 46.0

GC content: 30.86

Gene sequence:

>1011_bases
ATGAAAAACTTACCAAAAAATAATTATTCAAGAAAAAATAGAAAACAGGACAAAAATAATTCTGATTCTAATTTCTACTC
AAAAAACACAAATTCATCAAAAAAACATAATAGATTTCCCATCCATTCTTCTAAAAATCAAGGGGTTAACAATTTAGATG
AAAGTTATAAAAAGAAAGATAACTTTTCATATTTAAAAAAATCAAAGCCAATAAATAAATCTAATATTAAAGTCTCTTCC
AACTCTCAGGATATTTATCAAGACCTTGCAAATAAAAAAAATTTTGATGATTGGATATGGGGGAAACATTCGGTTTTTGA
GGCTCTTATTAATCAAAGAGCTATTAACAGGATTTGGTGTACCTCAGAAATCTTTTCTTCAGAGAAATTTTATATTTTAC
TCAAAGAACTTAAATCAAAAGGTGTCCTTATTGAAGAAGTTTCTTGGAATAGGCTTTCGCAATTGACTTTTGGTGCTTCA
CATCAAGGTGTCGCGTTGCAATTAGCATGCTCTAAAACAATTTCCCTAGAAAAATTAATCGATTTTTCTAAAAGGAACAC
TTCAAATCCAATTATTCTTGCCTTAGACGGAATTACTGATCCACATAATGTTGGTGCAATTATAAGATCAGCAGAGGCAT
TTGATTGTAAGGGTATAATAATTCCTCAAAGAAGATCTGCTGGATTAACAGGAACGGTTGCGAAGGTTGCTGCAGGAGCT
TTGGAACACTTGCCAGTAAGTAGAGTTGTTAATTTAAATAGGGCATTAGAAGAGCTAAAGAAAAATGGTTTTATGGTTAT
TGGATTATCTGGAGATGGTCAATTATCTATCTCAAAGTTTCATGAAAAGACTCCCTTGGTAGTAATAGTGGGAGCTGAAG
ATAAAGGTATTTCTTTGCTTACTCAAAAAAAATGCGATTTTTTATTAAAGATTCCTCTTAAAGGAAAAACATCAAGTTTA
AATGCATCTGTTGCAGCCGCAATTTCTCTTTTTCATTTGACAAATAAATAA

Upstream 100 bases:

>100_bases
GCAACTGGATTTGAAACCCTGATCGGTTGGCTATTTCTAAAAGACCCTCAAAGATTGTCAGCTCTTTTTGAGCACCTTGA
AATCAAAATGAATTGAATTA

Downstream 100 bases:

>100_bases
TTTTTTTGTTAAAAATAATTTTTATAATATTTTCTAAAATATTGTTGTTTCAATATATTTATATAATTAATGATAAATTA
TTGAAATATTACTACAAAAT

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKNLPKNNYSRKNRKQDKNNSDSNFYSKNTNSSKKHNRFPIHSSKNQGVNNLDESYKKKDNFSYLKKSKPINKSNIKVSS
NSQDIYQDLANKKNFDDWIWGKHSVFEALINQRAINRIWCTSEIFSSEKFYILLKELKSKGVLIEEVSWNRLSQLTFGAS
HQGVALQLACSKTISLEKLIDFSKRNTSNPIILALDGITDPHNVGAIIRSAEAFDCKGIIIPQRRSAGLTGTVAKVAAGA
LEHLPVSRVVNLNRALEELKKNGFMVIGLSGDGQLSISKFHEKTPLVVIVGAEDKGISLLTQKKCDFLLKIPLKGKTSSL
NASVAAAISLFHLTNK

Sequences:

>Translated_336_residues
MKNLPKNNYSRKNRKQDKNNSDSNFYSKNTNSSKKHNRFPIHSSKNQGVNNLDESYKKKDNFSYLKKSKPINKSNIKVSS
NSQDIYQDLANKKNFDDWIWGKHSVFEALINQRAINRIWCTSEIFSSEKFYILLKELKSKGVLIEEVSWNRLSQLTFGAS
HQGVALQLACSKTISLEKLIDFSKRNTSNPIILALDGITDPHNVGAIIRSAEAFDCKGIIIPQRRSAGLTGTVAKVAAGA
LEHLPVSRVVNLNRALEELKKNGFMVIGLSGDGQLSISKFHEKTPLVVIVGAEDKGISLLTQKKCDFLLKIPLKGKTSSL
NASVAAAISLFHLTNK
>Mature_336_residues
MKNLPKNNYSRKNRKQDKNNSDSNFYSKNTNSSKKHNRFPIHSSKNQGVNNLDESYKKKDNFSYLKKSKPINKSNIKVSS
NSQDIYQDLANKKNFDDWIWGKHSVFEALINQRAINRIWCTSEIFSSEKFYILLKELKSKGVLIEEVSWNRLSQLTFGAS
HQGVALQLACSKTISLEKLIDFSKRNTSNPIILALDGITDPHNVGAIIRSAEAFDCKGIIIPQRRSAGLTGTVAKVAAGA
LEHLPVSRVVNLNRALEELKKNGFMVIGLSGDGQLSISKFHEKTPLVVIVGAEDKGISLLTQKKCDFLLKIPLKGKTSSL
NASVAAAISLFHLTNK

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=191, Percent_Identity=31.413612565445, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=35.5371900826446, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1788935, Length=236, Percent_Identity=22.8813559322034, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17555908, Length=297, Percent_Identity=24.5791245791246, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324775, Length=395, Percent_Identity=27.3417721518987, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 37446; Mature: 37446

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLPKNNYSRKNRKQDKNNSDSNFYSKNTNSSKKHNRFPIHSSKNQGVNNLDESYKKKD
CCCCCCCCCCHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
NFSYLKKSKPINKSNIKVSSNSQDIYQDLANKKNFDDWIWGKHSVFEALINQRAINRIWC
CHHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHEE
TSEIFSSEKFYILLKELKSKGVLIEEVSWNRLSQLTFGASHQGVALQLACSKTISLEKLI
CHHHHCCCCEEEEEEHHHHCCEEEEECCCHHHHHHCCCCCCCCEEEEEECCCCCCHHHHH
DFSKRNTSNPIILALDGITDPHNVGAIIRSAEAFDCKGIIIPQRRSAGLTGTVAKVAAGA
HHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHH
LEHLPVSRVVNLNRALEELKKNGFMVIGLSGDGQLSISKFHEKTPLVVIVGAEDKGISLL
HHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHCCCCCEEEEECCCCCCCEEE
TQKKCDFLLKIPLKGKTSSLNASVAAAISLFHLTNK
ECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNLPKNNYSRKNRKQDKNNSDSNFYSKNTNSSKKHNRFPIHSSKNQGVNNLDESYKKKD
CCCCCCCCCCHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
NFSYLKKSKPINKSNIKVSSNSQDIYQDLANKKNFDDWIWGKHSVFEALINQRAINRIWC
CHHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHEE
TSEIFSSEKFYILLKELKSKGVLIEEVSWNRLSQLTFGASHQGVALQLACSKTISLEKLI
CHHHHCCCCEEEEEEHHHHCCEEEEECCCHHHHHHCCCCCCCCEEEEEECCCCCCHHHHH
DFSKRNTSNPIILALDGITDPHNVGAIIRSAEAFDCKGIIIPQRRSAGLTGTVAKVAAGA
HHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHH
LEHLPVSRVVNLNRALEELKKNGFMVIGLSGDGQLSISKFHEKTPLVVIVGAEDKGISLL
HHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHCCCCCEEEEECCCCCCCEEE
TQKKCDFLLKIPLKGKTSSLNASVAAAISLFHLTNK
ECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]