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The map label for this gene is carA [H]

Identifier: 78779232

GI number: 78779232

Start: 784306

End: 785445

Strand: Direct

Name: carA [H]

Synonym: PMT9312_0848

Alternate gene names: 78779232

Gene position: 784306-785445 (Clockwise)

Preceding gene: 78779231

Following gene: 78779233

Centisome position: 45.89

GC content: 31.14

Gene sequence:

>1140_bases
ATGATTAATTCCTATAAGAAAAATGCAAAATTAGTTTTAAGTAATGGATTGATATTTCCTGGATTTTCTTTTGGATATTC
GGGAACTGCTGTTGGCGAAATAGTATTTAATACTGGAATGACTGGGTATCAGGAGGTTATTACTGATCCAAGTTATTACG
GACAAATATTAACATTCACTTATCCAGAAGTTGGAAATACTGGTATTAATACTGAAGATTCAGAATCAAGTATTTGTGTT
AAAGGAATAATTGTTAGAAATTATTCAACAAATAATAGTAATTGGAGATCCCTGAAGAATTTTAATGAATGGTTAGTTGA
AAAAAAAATTATTGGTCTTTATGGAATTGATACACGAGCTCTTGTTAAAATTTTAAGATCTAATGGTTCGATGAATGCGG
TTCTTACCTCTGAAGAAAGAACTTTAGACAATTGCTTAGAAATAATAAATGAAACGCCAAAAATGGAGGGCTTGAATTTA
TCAAAAGAAGTTTCTACAAAGCAACAATATTTATGGCGGAATCCTACAGGAACAGATTTTGATGTTAGAAAAAGATATTC
TGAAAATACTAATAAACTAAAAGTAGTAGCAATTGATTTTGGAATAAAAAAATCAATTTTAAATAGACTAGTTTCTCATG
GTTGTGAAATTTTAGTTTTACCTTCAAGTTCTTCATTGAAAGATGTTATATCAAATAAGCCGGATGGTATATTCTTCTCA
AATGGTCCAGGAGATCCTTCTTCTGTTACTGAAGGTATAGATTTAGCAAGATCACTTATTGAATATGGTGAAATACCTAT
GTTTGGAATTTGCCTTGGCCATCAAATATTTGGAATAGCCCTAGGAGGTTCTACTTATAAACTACCTTTTGGACATCGTG
GTCTAAATCATCCTTGTGGAATCAATAATCAAATTGAGATAACAAGTCAGAATCATGGTTTTGCTCTTGACCCTAATTCT
CTCTCAAAGGACATAGTCAAAATCACTCACTATAACCTTAACGATAATACTGTTGCAGGATTGGAAGTTAATAATAAGCC
AATTTTTAGTGTTCAGTATCATCCAGAAGCAGGACCTGGTCCACATGACTCTGATTATTTATTTAAAAAATTTGTTTCTC
TAATGTTAGAAAGATGTTGA

Upstream 100 bases:

>100_bases
GTTTTAATAAAGCTTTATTTTCAATTAATCAAGGAAATCCTTGGGAGAAATTTTTACTCTTAAAAACTTATTTATCCTCA
GATGATTTAATTTCACCTTA

Downstream 100 bases:

>100_bases
CATATTGTTTTCTTTTGATTTGATTATTACATTTGATACATTAATTTTTTGGAGGAATTAGGTCATAGAAGATTTCCAAA
AGCTAACTGTTTCTTTAAGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MINSYKKNAKLVLSNGLIFPGFSFGYSGTAVGEIVFNTGMTGYQEVITDPSYYGQILTFTYPEVGNTGINTEDSESSICV
KGIIVRNYSTNNSNWRSLKNFNEWLVEKKIIGLYGIDTRALVKILRSNGSMNAVLTSEERTLDNCLEIINETPKMEGLNL
SKEVSTKQQYLWRNPTGTDFDVRKRYSENTNKLKVVAIDFGIKKSILNRLVSHGCEILVLPSSSSLKDVISNKPDGIFFS
NGPGDPSSVTEGIDLARSLIEYGEIPMFGICLGHQIFGIALGGSTYKLPFGHRGLNHPCGINNQIEITSQNHGFALDPNS
LSKDIVKITHYNLNDNTVAGLEVNNKPIFSVQYHPEAGPGPHDSDYLFKKFVSLMLERC

Sequences:

>Translated_379_residues
MINSYKKNAKLVLSNGLIFPGFSFGYSGTAVGEIVFNTGMTGYQEVITDPSYYGQILTFTYPEVGNTGINTEDSESSICV
KGIIVRNYSTNNSNWRSLKNFNEWLVEKKIIGLYGIDTRALVKILRSNGSMNAVLTSEERTLDNCLEIINETPKMEGLNL
SKEVSTKQQYLWRNPTGTDFDVRKRYSENTNKLKVVAIDFGIKKSILNRLVSHGCEILVLPSSSSLKDVISNKPDGIFFS
NGPGDPSSVTEGIDLARSLIEYGEIPMFGICLGHQIFGIALGGSTYKLPFGHRGLNHPCGINNQIEITSQNHGFALDPNS
LSKDIVKITHYNLNDNTVAGLEVNNKPIFSVQYHPEAGPGPHDSDYLFKKFVSLMLERC
>Mature_379_residues
MINSYKKNAKLVLSNGLIFPGFSFGYSGTAVGEIVFNTGMTGYQEVITDPSYYGQILTFTYPEVGNTGINTEDSESSICV
KGIIVRNYSTNNSNWRSLKNFNEWLVEKKIIGLYGIDTRALVKILRSNGSMNAVLTSEERTLDNCLEIINETPKMEGLNL
SKEVSTKQQYLWRNPTGTDFDVRKRYSENTNKLKVVAIDFGIKKSILNRLVSHGCEILVLPSSSSLKDVISNKPDGIFFS
NGPGDPSSVTEGIDLARSLIEYGEIPMFGICLGHQIFGIALGGSTYKLPFGHRGLNHPCGINNQIEITSQNHGFALDPNS
LSKDIVKITHYNLNDNTVAGLEVNNKPIFSVQYHPEAGPGPHDSDYLFKKFVSLMLERC

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI169790915, Length=389, Percent_Identity=37.7892030848329, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI21361331, Length=389, Percent_Identity=37.7892030848329, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=36.9109947643979, Blast_Score=231, Evalue=6e-61,
Organism=Escherichia coli, GI1786215, Length=374, Percent_Identity=42.5133689839572, Blast_Score=313, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI193204318, Length=380, Percent_Identity=37.3684210526316, Blast_Score=230, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6324878, Length=388, Percent_Identity=37.1134020618557, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=391, Percent_Identity=36.0613810741688, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24642586, Length=400, Percent_Identity=35.75, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI45555749, Length=400, Percent_Identity=35.75, Blast_Score=245, Evalue=4e-65,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41945; Mature: 41945

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSYKKNAKLVLSNGLIFPGFSFGYSGTAVGEIVFNTGMTGYQEVITDPSYYGQILTFT
CCCCCCCCCEEEEECCEECCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCHHCCEEEEEE
YPEVGNTGINTEDSESSICVKGIIVRNYSTNNSNWRSLKNFNEWLVEKKIIGLYGIDTRA
CCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEECCCHHH
LVKILRSNGSMNAVLTSEERTLDNCLEIINETPKMEGLNLSKEVSTKQQYLWRNPTGTDF
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCCH
DVRKRYSENTNKLKVVAIDFGIKKSILNRLVSHGCEILVLPSSSSLKDVISNKPDGIFFS
HHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCCEEEEC
NGPGDPSSVTEGIDLARSLIEYGEIPMFGICLGHQIFGIALGGSTYKLPFGHRGLNHPCG
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCEEECCCCCCCCCCCCC
INNQIEITSQNHGFALDPNSLSKDIVKITHYNLNDNTVAGLEVNNKPIFSVQYHPEAGPG
CCCEEEEEECCCCEEECCCHHCCCEEEEEEEECCCCEEEEEEECCCEEEEEEECCCCCCC
PHDSDYLFKKFVSLMLERC
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINSYKKNAKLVLSNGLIFPGFSFGYSGTAVGEIVFNTGMTGYQEVITDPSYYGQILTFT
CCCCCCCCCEEEEECCEECCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCHHCCEEEEEE
YPEVGNTGINTEDSESSICVKGIIVRNYSTNNSNWRSLKNFNEWLVEKKIIGLYGIDTRA
CCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEECCCHHH
LVKILRSNGSMNAVLTSEERTLDNCLEIINETPKMEGLNLSKEVSTKQQYLWRNPTGTDF
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCCH
DVRKRYSENTNKLKVVAIDFGIKKSILNRLVSHGCEILVLPSSSSLKDVISNKPDGIFFS
HHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCCEEEEC
NGPGDPSSVTEGIDLARSLIEYGEIPMFGICLGHQIFGIALGGSTYKLPFGHRGLNHPCG
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCEEECCCCCCCCCCCCC
INNQIEITSQNHGFALDPNSLSKDIVKITHYNLNDNTVAGLEVNNKPIFSVQYHPEAGPG
CCCEEEEEECCCCEEECCCHHCCCEEEEEEEECCCCEEEEEEECCCEEEEEEECCCCCCC
PHDSDYLFKKFVSLMLERC
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]