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The map label for this gene is ispD

Identifier: 78778839

GI number: 78778839

Start: 429230

End: 429910

Strand: Direct

Name: ispD

Synonym: PMT9312_0454

Alternate gene names: 78778839

Gene position: 429230-429910 (Clockwise)

Preceding gene: 78778837

Following gene: 78778842

Centisome position: 25.11

GC content: 33.48

Gene sequence:

>681_bases
GTGCACTTTTTAATACCAGCTGCAGGCAGTGGTAGCAGAATGAAAGCTGGGAAAAATAAATTACTTATTGATTTAGAGGG
AGAGTCTTTGATTTATTGGACACTTAAATCTGTATTTTCTGCAAGCTCAACAAACTGGGTTGGAATAATAGGGCAACCAA
AAGATAAAGAATTATTATTAAATTCAGCAAAGAACTTTGCCCATAAAGTTCATTGGATTAATGGTGGAGATACCCGACAA
GAGTCAGTTTTTAATGGTTTAAAGGCACTGCCCAAAGATGCTGAAAAAGTTTTAATTCATGATGGTGCTCGATGTCTAAT
TAATCCTGAATTGATAGACCAATGCGCAAACCAATTAGATCAAAATGAAGCTGTTATTTTGGCTACTAAGGTAACTGACA
CAATAAAGATTGTTGATAATGAAGGTTTTATTAAAGAAACACCAGATAGAAATTACTTATGGGCAGCGCAAACTCCTCAG
GGATTTTTAGTAGATAGATTAAAAAAAGCTCATACTATGGCAATTGATAAAAACTGGAAAGTCACAGATGATGCCTCACT
ATTCGAAATGCTTAATTGGAAAGTAAAAATCATTGAAGGAACTTATTCAAATATAAAAATTACGTCCCCTATAGATTTGA
AAATAGCAAAACTTTTTGTGAAGAACTCAACTCCTAGTTAA

Upstream 100 bases:

>100_bases
TTGGACATTGTGAAATTTTATAATTTTCAATCGTAGTACAATTTGTGTAAAGATATTGTTTTTATTTATAATTGTCTAGA
AAATATTAAGACCTTTTATC

Downstream 100 bases:

>100_bases
AAAGGTTTATTGATACTAAGAATGCCGTTATCGCCATTTAAGGTTGCTTCATACCCTAAAGGGATGCATGCATTCCCTGA
TATGTGGCCTATGGGGAGGT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MHFLIPAAGSGSRMKAGKNKLLIDLEGESLIYWTLKSVFSASSTNWVGIIGQPKDKELLLNSAKNFAHKVHWINGGDTRQ
ESVFNGLKALPKDAEKVLIHDGARCLINPELIDQCANQLDQNEAVILATKVTDTIKIVDNEGFIKETPDRNYLWAAQTPQ
GFLVDRLKKAHTMAIDKNWKVTDDASLFEMLNWKVKIIEGTYSNIKITSPIDLKIAKLFVKNSTPS

Sequences:

>Translated_226_residues
MHFLIPAAGSGSRMKAGKNKLLIDLEGESLIYWTLKSVFSASSTNWVGIIGQPKDKELLLNSAKNFAHKVHWINGGDTRQ
ESVFNGLKALPKDAEKVLIHDGARCLINPELIDQCANQLDQNEAVILATKVTDTIKIVDNEGFIKETPDRNYLWAAQTPQ
GFLVDRLKKAHTMAIDKNWKVTDDASLFEMLNWKVKIIEGTYSNIKITSPIDLKIAKLFVKNSTPS
>Mature_226_residues
MHFLIPAAGSGSRMKAGKNKLLIDLEGESLIYWTLKSVFSASSTNWVGIIGQPKDKELLLNSAKNFAHKVHWINGGDTRQ
ESVFNGLKALPKDAEKVLIHDGARCLINPELIDQCANQLDQNEAVILATKVTDTIKIVDNEGFIKETPDRNYLWAAQTPQ
GFLVDRLKKAHTMAIDKNWKVTDDASLFEMLNWKVKIIEGTYSNIKITSPIDLKIAKLFVKNSTPS

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=235, Percent_Identity=24.2553191489362, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789104, Length=222, Percent_Identity=32.4324324324324, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_PROM9 (Q31C80)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_396951.1
- ProteinModelPortal:   Q31C80
- SMR:   Q31C80
- STRING:   Q31C80
- GeneID:   3765251
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0454
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- ProtClustDB:   PRK00155
- BioCyc:   PMAR74546:PMT9312_0454-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25218; Mature: 25218

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFLIPAAGSGSRMKAGKNKLLIDLEGESLIYWTLKSVFSASSTNWVGIIGQPKDKELLL
CEEEEECCCCCCCEECCCCEEEEEECCCEEEEEEHHHHHCCCCCCEEEEEECCCCHHHHH
NSAKNFAHKVHWINGGDTRQESVFNGLKALPKDAEKVLIHDGARCLINPELIDQCANQLD
HHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCEEEECHHHHHHHHHHCC
QNEAVILATKVTDTIKIVDNEGFIKETPDRNYLWAAQTPQGFLVDRLKKAHTMAIDKNWK
CCCEEEEEEECCEEEEEEECCCCEEECCCCCEEEEECCCCCHHHHHHHHHHEEEECCCCC
VTDDASLFEMLNWKVKIIEGTYSNIKITSPIDLKIAKLFVKNSTPS
CCCCHHHHHHHCCEEEEEECCCCCEEECCCCCEEEEEEEEECCCCC
>Mature Secondary Structure
MHFLIPAAGSGSRMKAGKNKLLIDLEGESLIYWTLKSVFSASSTNWVGIIGQPKDKELLL
CEEEEECCCCCCCEECCCCEEEEEECCCEEEEEEHHHHHCCCCCCEEEEEECCCCHHHHH
NSAKNFAHKVHWINGGDTRQESVFNGLKALPKDAEKVLIHDGARCLINPELIDQCANQLD
HHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCEEEECHHHHHHHHHHCC
QNEAVILATKVTDTIKIVDNEGFIKETPDRNYLWAAQTPQGFLVDRLKKAHTMAIDKNWK
CCCEEEEEEECCEEEEEEECCCCEEECCCCCEEEEECCCCCHHHHHHHHHHEEEECCCCC
VTDDASLFEMLNWKVKIIEGTYSNIKITSPIDLKIAKLFVKNSTPS
CCCCHHHHHHHCCEEEEEECCCCCEEECCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA