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The map label for this gene is hisF [H]

Identifier: 78778814

GI number: 78778814

Start: 405111

End: 405881

Strand: Direct

Name: hisF [H]

Synonym: PMT9312_0429

Alternate gene names: 78778814

Gene position: 405111-405881 (Clockwise)

Preceding gene: 78778810

Following gene: 78778815

Centisome position: 23.7

GC content: 36.32

Gene sequence:

>771_bases
ATGGTAGCTCTTCGTTTAATTCCTTGTTTAGATGTCGCTCATGGCAGAGTGGTTAAAGGTGTAAATTTTGTTAACTTGAG
AGACTCAGGCGATCCTGTTGAATTGGCTTGTAGGTATTCTGATGAGGGCGCAGATGAATTAGTATTCTTAGATATTAGAG
CTAGTGTAGAAAATAGAAATACATTAGTTGACCTTGTCTCTAGGACCGCAAAATCAGTAAAAATCCCATTTACAGTAGGT
GGAGGAATAGATTCTGTTTCTTCAATTAATGATCTTTTAAGAGCTGGAGCGGACAAAGTGAGTTTGAATTCTTCTGCTGT
TAGAAATCCAGATTTAATTTCTAAAAGTTCTAGAGAATTTGGTAATCAATGTATCGTGATAGCAATTGATGCTAAAAGAA
AAGTGAATAAAACTGATGAATGGGAGGTATATGTAAAAGGGGGTAGAGAAAATACTGGAATAGATGTATTAAGTTGGGCA
AAGAAAGTTGAGGAGTTAGGCGCAGGGGAAATTTTGCTTACTTCAATGGATGGTGATGGCACGCAGAATGGATATGATTT
ACATCTGACTGAATCTGTTGCCAATATTGTTAATATTCCAGTGATTGCTTCTGGAGGAGCAGGTTGTTTAGAAGATATCT
ATGATGTTTTCAATGAAGGCAGGGCATCTGCCGCACTTTTAGCATCATTACTTCATGATAAGAAACTTTCTTTAAGAGAA
ATAAAGACTTTCCTCCTCGAAAGAAAACTTCCAATTAGACCATATGAATAA

Upstream 100 bases:

>100_bases
ATTTTACTACCGATTTTGGGTAATAATTTTGTTTCAGCCATAAGAGGTAAAATGGAAGCTATTATGCAAGATTAACAGCA
TTTGGACAATTTTTACTAAA

Downstream 100 bases:

>100_bases
AAAATTTAATACCAAAAAGAAATAAGTTAAAAATTTAAAAATGAAATTCACAAAAACTATCGAAGTCAAAAATATATTTA
ATAAAATTTCTTATAAATAT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MVALRLIPCLDVAHGRVVKGVNFVNLRDSGDPVELACRYSDEGADELVFLDIRASVENRNTLVDLVSRTAKSVKIPFTVG
GGIDSVSSINDLLRAGADKVSLNSSAVRNPDLISKSSREFGNQCIVIAIDAKRKVNKTDEWEVYVKGGRENTGIDVLSWA
KKVEELGAGEILLTSMDGDGTQNGYDLHLTESVANIVNIPVIASGGAGCLEDIYDVFNEGRASAALLASLLHDKKLSLRE
IKTFLLERKLPIRPYE

Sequences:

>Translated_256_residues
MVALRLIPCLDVAHGRVVKGVNFVNLRDSGDPVELACRYSDEGADELVFLDIRASVENRNTLVDLVSRTAKSVKIPFTVG
GGIDSVSSINDLLRAGADKVSLNSSAVRNPDLISKSSREFGNQCIVIAIDAKRKVNKTDEWEVYVKGGRENTGIDVLSWA
KKVEELGAGEILLTSMDGDGTQNGYDLHLTESVANIVNIPVIASGGAGCLEDIYDVFNEGRASAALLASLLHDKKLSLRE
IKTFLLERKLPIRPYE
>Mature_256_residues
MVALRLIPCLDVAHGRVVKGVNFVNLRDSGDPVELACRYSDEGADELVFLDIRASVENRNTLVDLVSRTAKSVKIPFTVG
GGIDSVSSINDLLRAGADKVSLNSSAVRNPDLISKSSREFGNQCIVIAIDAKRKVNKTDEWEVYVKGGRENTGIDVLSWA
KKVEELGAGEILLTSMDGDGTQNGYDLHLTESVANIVNIPVIASGGAGCLEDIYDVFNEGRASAALLASLLHDKKLSLRE
IKTFLLERKLPIRPYE

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=43.579766536965, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=27.1966527196653, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=310, Percent_Identity=33.5483870967742, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27769; Mature: 27769

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVALRLIPCLDVAHGRVVKGVNFVNLRDSGDPVELACRYSDEGADELVFLDIRASVENRN
CCEEEEECHHHCCCCCEECCEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCCH
TLVDLVSRTAKSVKIPFTVGGGIDSVSSINDLLRAGADKVSLNSSAVRNPDLISKSSREF
HHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCHHHH
GNQCIVIAIDAKRKVNKTDEWEVYVKGGRENTGIDVLSWAKKVEELGAGEILLTSMDGDG
CCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
TQNGYDLHLTESVANIVNIPVIASGGAGCLEDIYDVFNEGRASAALLASLLHDKKLSLRE
CCCCEEEEEHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHH
IKTFLLERKLPIRPYE
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVALRLIPCLDVAHGRVVKGVNFVNLRDSGDPVELACRYSDEGADELVFLDIRASVENRN
CCEEEEECHHHCCCCCEECCEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCCH
TLVDLVSRTAKSVKIPFTVGGGIDSVSSINDLLRAGADKVSLNSSAVRNPDLISKSSREF
HHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCHHHH
GNQCIVIAIDAKRKVNKTDEWEVYVKGGRENTGIDVLSWAKKVEELGAGEILLTSMDGDG
CCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
TQNGYDLHLTESVANIVNIPVIASGGAGCLEDIYDVFNEGRASAALLASLLHDKKLSLRE
CCCCEEEEEHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHH
IKTFLLERKLPIRPYE
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA