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The map label for this gene is trmB

Identifier: 78484464

GI number: 78484464

Start: 152188

End: 152910

Strand: Reverse

Name: trmB

Synonym: Tcr_0119

Alternate gene names: 78484464

Gene position: 152910-152188 (Counterclockwise)

Preceding gene: 78484468

Following gene: 78484461

Centisome position: 6.3

GC content: 46.06

Gene sequence:

>723_bases
ATGACTGAAAATACAGAATCCCCTCAAAGTGTTGCAACCGATATTCCGGAACATAAACGACGCATCAAAAGCTTTGTGTT
ACGCCAAGGTCGCTTATCTCAGGCACAACAAAATGCCATTGATACGATGTGGCCTCAATACGGCTTAACATTAGAAGACA
AGCTACTGGATTTCAACACCTTGTTTGGTCGGGAAGCCCCCACCATTATTGAAATTGGCTTTGGAATGGGAAACAGTCTG
GCAGCCATGGCTGAAGCGCACCCTGAAAACAATTACATTGGCATTGAAGTCCATCGACCTGGCGTCGGGGCGCTGTTAAA
ACTGGTGGCCGAAAAAGGACTCACCAATGTTCGTGTGTTTAATGAAGATGCAATAGAGGTACTCAACCGACAGATTCCTA
AAAACAGCCTGTCAGCCGTGTATTTATTTTTCCCCGATCCTTGGCACAAAACCAAACACAAAAAACGCCGCATTTTACAG
GCCGAATTCGCTGAAAAAATTGCTCAATATCTCGCACCCGGCGGACAATTCCATATGGCAACGGATTGGGAAGACTATGC
CTTGCATATGATGTCAGTGATGAGCGCTGCGAAAGGCTATCGTAATATCTCTGGAGAAGGCCAATATACCCCTCGCCCTG
ATTATCGCCCTTTAACCAAGTTTGAACAGAGAGGTCACCGCTTAGGTCATGGGGTTTGGGACTTGGTCTTTGAACGAATC
TAA

Upstream 100 bases:

>100_bases
TTTGCGAAAATTCTTCTAAGTCTGACATAGCGTTGCTTTAAGTTCTTCTGAATTGAGCTATAATAGCCGGAAAATTAAAC
TGCCATTGTACTAGAACCCT

Downstream 100 bases:

>100_bases
CCGTCTCATCCCTTAAAAAATGGCCAGGCCTGGCCATTTTAATCCCTCACGATTGAATCTTTTATGAAGCCTTCAAAAAT
TGCTGCAAGCTGTTCAACAG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTENTESPQSVATDIPEHKRRIKSFVLRQGRLSQAQQNAIDTMWPQYGLTLEDKLLDFNTLFGREAPTIIEIGFGMGNSL
AAMAEAHPENNYIGIEVHRPGVGALLKLVAEKGLTNVRVFNEDAIEVLNRQIPKNSLSAVYLFFPDPWHKTKHKKRRILQ
AEFAEKIAQYLAPGGQFHMATDWEDYALHMMSVMSAAKGYRNISGEGQYTPRPDYRPLTKFEQRGHRLGHGVWDLVFERI

Sequences:

>Translated_240_residues
MTENTESPQSVATDIPEHKRRIKSFVLRQGRLSQAQQNAIDTMWPQYGLTLEDKLLDFNTLFGREAPTIIEIGFGMGNSL
AAMAEAHPENNYIGIEVHRPGVGALLKLVAEKGLTNVRVFNEDAIEVLNRQIPKNSLSAVYLFFPDPWHKTKHKKRRILQ
AEFAEKIAQYLAPGGQFHMATDWEDYALHMMSVMSAAKGYRNISGEGQYTPRPDYRPLTKFEQRGHRLGHGVWDLVFERI
>Mature_239_residues
TENTESPQSVATDIPEHKRRIKSFVLRQGRLSQAQQNAIDTMWPQYGLTLEDKLLDFNTLFGREAPTIIEIGFGMGNSLA
AMAEAHPENNYIGIEVHRPGVGALLKLVAEKGLTNVRVFNEDAIEVLNRQIPKNSLSAVYLFFPDPWHKTKHKKRRILQA
EFAEKIAQYLAPGGQFHMATDWEDYALHMMSVMSAAKGYRNISGEGQYTPRPDYRPLTKFEQRGHRLGHGVWDLVFERI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=221, Percent_Identity=60.6334841628959, Blast_Score=294, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_THICR (Q31JF8)

Other databases:

- EMBL:   CP000109
- RefSeq:   YP_390389.1
- ProteinModelPortal:   Q31JF8
- SMR:   Q31JF8
- STRING:   Q31JF8
- GeneID:   3762527
- GenomeReviews:   CP000109_GR
- KEGG:   tcx:Tcr_0119
- NMPDR:   fig|317025.3.peg.135
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   Q31JF8
- BioCyc:   TCRU317025:TCR_0119-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27350; Mature: 27218

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: BINDING 71-71 BINDING 96-96 BINDING 123-123 BINDING 146-146 BINDING 150-150 BINDING 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENTESPQSVATDIPEHKRRIKSFVLRQGRLSQAQQNAIDTMWPQYGLTLEDKLLDFNT
CCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LFGREAPTIIEIGFGMGNSLAAMAEAHPENNYIGIEVHRPGVGALLKLVAEKGLTNVRVF
HHCCCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEE
NEDAIEVLNRQIPKNSLSAVYLFFPDPWHKTKHKKRRILQAEFAEKIAQYLAPGGQFHMA
CHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TDWEDYALHMMSVMSAAKGYRNISGEGQYTPRPDYRPLTKFEQRGHRLGHGVWDLVFERI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
TENTESPQSVATDIPEHKRRIKSFVLRQGRLSQAQQNAIDTMWPQYGLTLEDKLLDFNT
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LFGREAPTIIEIGFGMGNSLAAMAEAHPENNYIGIEVHRPGVGALLKLVAEKGLTNVRVF
HHCCCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEE
NEDAIEVLNRQIPKNSLSAVYLFFPDPWHKTKHKKRRILQAEFAEKIAQYLAPGGQFHMA
CHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TDWEDYALHMMSVMSAAKGYRNISGEGQYTPRPDYRPLTKFEQRGHRLGHGVWDLVFERI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA