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The map label for this gene is lacA [C]

Identifier: 78358719

GI number: 78358719

Start: 3641739

End: 3642338

Strand: Reverse

Name: lacA [C]

Synonym: Dde_3680

Alternate gene names: 78358719

Gene position: 3642338-3641739 (Counterclockwise)

Preceding gene: 78358720

Following gene: 78358718

Centisome position: 97.64

GC content: 62.33

Gene sequence:

>600_bases
ATGATGTCTTCAGAGATGCAGCCCCCGCAGCACGGCCCGGATGAAAGACGTTTTCAGAGCCGCGTGCGCACGGCGGCGTC
CGGCGGGCTGTGGCACGACATGCGCCGCTGGTGGCAGCTGCGCCGCGTGGGCAGTGCCGGCTGCGGCATACACATGGACA
GCGGTGTGCATCTGATGCGGTACACGCGGCATATGCATTTCGGCGACAATGTGTTTTTCAAACAGGGCGCGCGGCTGTGT
GTGGCTCAGCCGCAGGGTGAACTGCGCATCGGCAGCAATACGACAATAGGATATCACACGACTATTTTTGCAAGCTACTC
TGTAGTGATAGGGGCGGACTGCCTCATAGCGCCCTTCTGTTATCTGGTGGATGCCGACCACGGCATTGCGGCGGGCAGCA
GAATCAACACGCAGGATATGACCGCCCGCCCCGTGGTGGTGGGCGATGATGTGTGGCTGGGGGCGCGTGTGACCGTGGTT
TCGGGGGTGCGTATAGGAACCGGCGCTGTGGCGGGGGCCGGCAGCGTCATTACTGCCGATGTGCCTGCAGGGGCCATCGT
GGCCGGTGCTCCGGCACGGATAGTGGGATACAGAAAATGA

Upstream 100 bases:

>100_bases
GCTACGAGGATTTTCTGGGGCGCAGGGCGCCGCGCAGCATGGGAGCGCACGAGGTGCTTACGGCTGCCGATCTGCAGGCC
GTGCTGGAACAGGAGTGATC

Downstream 100 bases:

>100_bases
ATGCGGTGCTGCAGAAAAAAGGCGGTGTCGGAATAATCATTCTGGCGCGGCTTGATTCGACCCGTCTGCCGGGCAAGGCG
TTGCGTACGGCTGCCGGCAA

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MMSSEMQPPQHGPDERRFQSRVRTAASGGLWHDMRRWWQLRRVGSAGCGIHMDSGVHLMRYTRHMHFGDNVFFKQGARLC
VAQPQGELRIGSNTTIGYHTTIFASYSVVIGADCLIAPFCYLVDADHGIAAGSRINTQDMTARPVVVGDDVWLGARVTVV
SGVRIGTGAVAGAGSVITADVPAGAIVAGAPARIVGYRK

Sequences:

>Translated_199_residues
MMSSEMQPPQHGPDERRFQSRVRTAASGGLWHDMRRWWQLRRVGSAGCGIHMDSGVHLMRYTRHMHFGDNVFFKQGARLC
VAQPQGELRIGSNTTIGYHTTIFASYSVVIGADCLIAPFCYLVDADHGIAAGSRINTQDMTARPVVVGDDVWLGARVTVV
SGVRIGTGAVAGAGSVITADVPAGAIVAGAPARIVGYRK
>Mature_199_residues
MMSSEMQPPQHGPDERRFQSRVRTAASGGLWHDMRRWWQLRRVGSAGCGIHMDSGVHLMRYTRHMHFGDNVFFKQGARLC
VAQPQGELRIGSNTTIGYHTTIFASYSVVIGADCLIAPFCYLVDADHGIAAGSRINTQDMTARPVVVGDDVWLGARVTVV
SGVRIGTGAVAGAGSVITADVPAGAIVAGAPARIVGYRK

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=108, Percent_Identity=37.037037037037, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1788345, Length=139, Percent_Identity=33.8129496402878, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786664, Length=106, Percent_Identity=33.9622641509434, Blast_Score=72, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322243, Length=109, Percent_Identity=34.8623853211009, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 21379; Mature: 21379

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSSEMQPPQHGPDERRFQSRVRTAASGGLWHDMRRWWQLRRVGSAGCGIHMDSGVHLMR
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHH
YTRHMHFGDNVFFKQGARLCVAQPQGELRIGSNTTIGYHTTIFASYSVVIGADCLIAPFC
HHHHHCCCCCEEECCCCEEEEECCCCCEEECCCCEEEEEEEEEEEEEEEECCHHHHHHHH
YLVDADHGIAAGSRINTQDMTARPVVVGDDVWLGARVTVVSGVRIGTGAVAGAGSVITAD
EEEECCCCCCCCCCCCCCCCCCCCEEECCCEEECEEEEEEECEEECCCCCCCCCCEEEEC
VPAGAIVAGAPARIVGYRK
CCCCEEEECCCHHEEECCC
>Mature Secondary Structure
MMSSEMQPPQHGPDERRFQSRVRTAASGGLWHDMRRWWQLRRVGSAGCGIHMDSGVHLMR
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHH
YTRHMHFGDNVFFKQGARLCVAQPQGELRIGSNTTIGYHTTIFASYSVVIGADCLIAPFC
HHHHHCCCCCEEECCCCEEEEECCCCCEEECCCCEEEEEEEEEEEEEEEECCHHHHHHHH
YLVDADHGIAAGSRINTQDMTARPVVVGDDVWLGARVTVVSGVRIGTGAVAGAGSVITAD
EEEECCCCCCCCCCCCCCCCCCCCEEECCCEEECEEEEEEECEEECCCCCCCCCCEEEEC
VPAGAIVAGAPARIVGYRK
CCCCEEEECCCHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]