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The map label for this gene is potB [H]

Identifier: 78358710

GI number: 78358710

Start: 3631320

End: 3632198

Strand: Direct

Name: potB [H]

Synonym: Dde_3671

Alternate gene names: 78358710

Gene position: 3631320-3632198 (Clockwise)

Preceding gene: 78358709

Following gene: 78358711

Centisome position: 97.35

GC content: 56.43

Gene sequence:

>879_bases
ATGATGGGGAGTAAGTTTTTCCGCCGGTTCAGCATCACCGCCGTTGCGCTGTGGCTGGGGCTTTTTGCGCTGGTACCGCA
CCTTATGCTGGTGGGAGCGTCTTTTCTGACGCGCGACCCCGGCAGCTTTGTTGCGCCGCGACTCACTCTGGACAACTTTA
TCCGTCTGTTCGATCCCATGTTCGTCAGAATATTTCTGGAATCGTTCAGGCTGGCGGGCATCACCACGGCTTTCTGCCTG
CTGATGGGCTATCCGTTCGCCTTTCTGCTGGCCACAGCCCGCGAACACCTGCGCCCGTGGCTGCTGCTGCTTGTCATCAT
TCCTTTCTGGACAAACTCGCTCATCCGCACCTACGCCATGATCATTATCCTGAAATCCAACGGGGTGCTGAGCAAAGTGC
TCATGGCGGCGGGGCTTATTGATGCCCCCATATCGCTCATGTACAGCGAAATAGCCGTGGTGGCCGGTTTTGCCTACACG
TCGCTTCCGTTCATGATTCTGCCGCTGTTCGCTTCCATCGAAAAGCTGGACATGCGCCTGCTGGATGCCGCACGCGACCT
TGGCGCCAGCACGCGGCAGACGTTTTTTTCCGTCACGCTGCCCCTGACCATGCCGGGCATAGTGGCCGGCTGCATGCTTG
TTTTTCTGCCTGTGCTGGGCTGTTTTTATGTGCCGGAAATACTGGGCGGTTCCAAAACGCTGGTCATGGGCAGCTTTATC
AAAAACCAGTTTCTGGTGGCCCGCGACTGGCCGCTGGGTTCCGCTGCCAGCATAGTGATGACTCTTATCCTCGTGGTACT
TATTCTGCTGTACTATATGGCAAAAAAACGCAGCGCCACGCATCACAAGGATGATGAACTGTACGGGGAGGGCATGTGA

Upstream 100 bases:

>100_bases
GTTGCAGAATTCTTCAACGAGGATGACGAAGAAATCAACTACACTCCGGGCGAACGCGTGGCCGTCACCTGGGTAAACGG
CTGGGAGGTAGTGCTGGGAG

Downstream 100 bases:

>100_bases
TGAAAATCCTGCGCCGGACCTACATGTGGGCGGTCTACGCCTTTCTGTACATTCCCATTCTGGTGGTCATTGCCTATTCC
GTCAACAACGCGAAATACAC

Product: polyamine ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MMGSKFFRRFSITAVALWLGLFALVPHLMLVGASFLTRDPGSFVAPRLTLDNFIRLFDPMFVRIFLESFRLAGITTAFCL
LMGYPFAFLLATAREHLRPWLLLLVIIPFWTNSLIRTYAMIIILKSNGVLSKVLMAAGLIDAPISLMYSEIAVVAGFAYT
SLPFMILPLFASIEKLDMRLLDAARDLGASTRQTFFSVTLPLTMPGIVAGCMLVFLPVLGCFYVPEILGGSKTLVMGSFI
KNQFLVARDWPLGSAASIVMTLILVVLILLYYMAKKRSATHHKDDELYGEGM

Sequences:

>Translated_292_residues
MMGSKFFRRFSITAVALWLGLFALVPHLMLVGASFLTRDPGSFVAPRLTLDNFIRLFDPMFVRIFLESFRLAGITTAFCL
LMGYPFAFLLATAREHLRPWLLLLVIIPFWTNSLIRTYAMIIILKSNGVLSKVLMAAGLIDAPISLMYSEIAVVAGFAYT
SLPFMILPLFASIEKLDMRLLDAARDLGASTRQTFFSVTLPLTMPGIVAGCMLVFLPVLGCFYVPEILGGSKTLVMGSFI
KNQFLVARDWPLGSAASIVMTLILVVLILLYYMAKKRSATHHKDDELYGEGM
>Mature_292_residues
MMGSKFFRRFSITAVALWLGLFALVPHLMLVGASFLTRDPGSFVAPRLTLDNFIRLFDPMFVRIFLESFRLAGITTAFCL
LMGYPFAFLLATAREHLRPWLLLLVIIPFWTNSLIRTYAMIIILKSNGVLSKVLMAAGLIDAPISLMYSEIAVVAGFAYT
SLPFMILPLFASIEKLDMRLLDAARDLGASTRQTFFSVTLPLTMPGIVAGCMLVFLPVLGCFYVPEILGGSKTLVMGSFI
KNQFLVARDWPLGSAASIVMTLILVVLILLYYMAKKRSATHHKDDELYGEGM

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=265, Percent_Identity=51.3207547169811, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1787080, Length=236, Percent_Identity=43.6440677966102, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787713, Length=276, Percent_Identity=31.8840579710145, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1786980, Length=96, Percent_Identity=37.5, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32458; Mature: 32458

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]