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The map label for this gene is prfC

Identifier: 78358688

GI number: 78358688

Start: 3611324

End: 3612910

Strand: Direct

Name: prfC

Synonym: Dde_3649

Alternate gene names: 78358688

Gene position: 3611324-3612910 (Clockwise)

Preceding gene: 78358687

Following gene: 78358689

Centisome position: 96.81

GC content: 57.91

Gene sequence:

>1587_bases
GTGAACAGGGAACATCAGAGAGAAGTAGACAAACGCCGCACGTTCGGCATCATCAGCCACCCCGACGCAGGCAAGACGAC
CCTTACCGAAAAGCTGCTGCTTTTCGGCGGGGCCATACAGATGGCCGGCACCGTCAAGTCGCGCAAGGCAGCCCGCCACG
CCACGTCAGACTGGATGGCCATGGAGCAGGAACGCGGCATATCCGTCACCACCTCGGTGATGAAGTTCCACCACAACGGC
TACGAAATAAATCTTCTCGATACCCCCGGTCACCAGGATTTCTCGGAAGACACCTACCGGGTGCTCACCGCGGTGGACAG
CGCGCTGCTGGTCATCGACTCCGCCAAAGGCGTGGAAGCGCAGACCCTGAAGCTGATGGATGTGTGCCGCATGCGCAGCA
CTCCCATTGTCACCTTCATCAACAAGCTGGACCGTGACGGCCTGCATCCGCTGGAAGTGATGGATGACATTGAAAGCCGC
CTCAATATCCAGTGCAGTCCGCTTACCTGGCCCATCGGCATGGGAGCGGATTTTAAAGGCACATGGGACATACGCACCGG
ACGGCTGCATATGTTCACCCCCGCCAACGACGGGCGCATCGCCTCGGGGCGCATCATTGAAGGACTGGACAACCCGGAAC
TGGACGAAGCGCTGGGAGAACAGGCCGTTACGCTGCGTGACGAGCTGGCGCTGCTGGCAGAAGCCGGAAACCCCTTTGAT
AAGGAACGGTACCTCAAAGGTGAACTGACCCCGGTGTTTTTCGGTTCGGCCATCAACAATTTCGGTGTGCGTGAAATGCT
GGACACCTTTGTGGATCTGGCACCGGCTCCCCAGCCGCGTCCGGCCACAACCCGCACGGTTTCACCCTATGAAGAAGATT
TTTCCGGTGTGGTTTTTAAGATTCAGGCAAACATGGACAAAAACCACCGCGACCGCATCGCCTTCATGCGTATCTGCTCC
GGCAAGTTCACACGCGGCATGAAAGTGCGTCATCACCGCATCGGCAAAGAAGTGGCACTGGCCAATGCCACCATATTCAT
GGCGCAGGACAGAACCGGCGTGGAAGAGGCATGGCCCGGCGACATCATAGGCATACACAATCACGGCACCATCAAGATAG
GGGACACTTTTTCCCTTCGCGAGCCGCTGAAATTTGTCGGCATACCCAACTTTGCCCCCGAACATTTCCGCAGGGTGGTG
CTGCGCGACCCCATGAAGGCCAAACAACTGCAAAAAGGTCTGGAGCAGCTGGCCGAAGAAGGCGCCGTACAGCTTTTCCG
GCCGCTTCTCGGCAATGACTATATTCTCGGCGCCGTGGGTGTGCTGCAGTTTGAAGTCATCGTGGCCCGGCTAAAAGACG
AATACAACGTCAACGCCCTGTATGAAGGCTGCAACATAACCACGGCACGCTGGCTGCACACCGACGACCCCAAAACCATG
GCCGAATTCCGCGACTACTACCGCAGCGAACTGGCCATGGACGCGGAAGGCTGCCTTGCCTATCTTGCTCCCAACCCGTG
GCGGCTTGAATCCGCAGTGGAGCGCTATCCCAAAATGGAATTCCGCACCACGCGTGAAATCAGCTAG

Upstream 100 bases:

>100_bases
TCATGCCTTATGTTGACCGCACACCGCATCTATAGCATAACACCGCCCTCGCGGGTGTCCCTTTCAGGGCGCCGCAAAAC
CACCCCGCCGAGGTTTCACC

Downstream 100 bases:

>100_bases
CACTGCGTCCGCCGAGGGCAGTTCCGCCGCGGCAACATCAGCGAGGTACTCCGCCATGCGGTCACACAGCGTTCTTTTTA
TTTTCTGCACCTGCCTTGCC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MNREHQREVDKRRTFGIISHPDAGKTTLTEKLLLFGGAIQMAGTVKSRKAARHATSDWMAMEQERGISVTTSVMKFHHNG
YEINLLDTPGHQDFSEDTYRVLTAVDSALLVIDSAKGVEAQTLKLMDVCRMRSTPIVTFINKLDRDGLHPLEVMDDIESR
LNIQCSPLTWPIGMGADFKGTWDIRTGRLHMFTPANDGRIASGRIIEGLDNPELDEALGEQAVTLRDELALLAEAGNPFD
KERYLKGELTPVFFGSAINNFGVREMLDTFVDLAPAPQPRPATTRTVSPYEEDFSGVVFKIQANMDKNHRDRIAFMRICS
GKFTRGMKVRHHRIGKEVALANATIFMAQDRTGVEEAWPGDIIGIHNHGTIKIGDTFSLREPLKFVGIPNFAPEHFRRVV
LRDPMKAKQLQKGLEQLAEEGAVQLFRPLLGNDYILGAVGVLQFEVIVARLKDEYNVNALYEGCNITTARWLHTDDPKTM
AEFRDYYRSELAMDAEGCLAYLAPNPWRLESAVERYPKMEFRTTREIS

Sequences:

>Translated_528_residues
MNREHQREVDKRRTFGIISHPDAGKTTLTEKLLLFGGAIQMAGTVKSRKAARHATSDWMAMEQERGISVTTSVMKFHHNG
YEINLLDTPGHQDFSEDTYRVLTAVDSALLVIDSAKGVEAQTLKLMDVCRMRSTPIVTFINKLDRDGLHPLEVMDDIESR
LNIQCSPLTWPIGMGADFKGTWDIRTGRLHMFTPANDGRIASGRIIEGLDNPELDEALGEQAVTLRDELALLAEAGNPFD
KERYLKGELTPVFFGSAINNFGVREMLDTFVDLAPAPQPRPATTRTVSPYEEDFSGVVFKIQANMDKNHRDRIAFMRICS
GKFTRGMKVRHHRIGKEVALANATIFMAQDRTGVEEAWPGDIIGIHNHGTIKIGDTFSLREPLKFVGIPNFAPEHFRRVV
LRDPMKAKQLQKGLEQLAEEGAVQLFRPLLGNDYILGAVGVLQFEVIVARLKDEYNVNALYEGCNITTARWLHTDDPKTM
AEFRDYYRSELAMDAEGCLAYLAPNPWRLESAVERYPKMEFRTTREIS
>Mature_528_residues
MNREHQREVDKRRTFGIISHPDAGKTTLTEKLLLFGGAIQMAGTVKSRKAARHATSDWMAMEQERGISVTTSVMKFHHNG
YEINLLDTPGHQDFSEDTYRVLTAVDSALLVIDSAKGVEAQTLKLMDVCRMRSTPIVTFINKLDRDGLHPLEVMDDIESR
LNIQCSPLTWPIGMGADFKGTWDIRTGRLHMFTPANDGRIASGRIIEGLDNPELDEALGEQAVTLRDELALLAEAGNPFD
KERYLKGELTPVFFGSAINNFGVREMLDTFVDLAPAPQPRPATTRTVSPYEEDFSGVVFKIQANMDKNHRDRIAFMRICS
GKFTRGMKVRHHRIGKEVALANATIFMAQDRTGVEEAWPGDIIGIHNHGTIKIGDTFSLREPLKFVGIPNFAPEHFRRVV
LRDPMKAKQLQKGLEQLAEEGAVQLFRPLLGNDYILGAVGVLQFEVIVARLKDEYNVNALYEGCNITTARWLHTDDPKTM
AEFRDYYRSELAMDAEGCLAYLAPNPWRLESAVERYPKMEFRTTREIS

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=495, Percent_Identity=26.8686868686869, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI25306283, Length=334, Percent_Identity=32.0359281437126, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI19923640, Length=334, Percent_Identity=32.3353293413174, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI25306287, Length=293, Percent_Identity=33.1058020477816, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI157426893, Length=176, Percent_Identity=31.8181818181818, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI94966754, Length=144, Percent_Identity=32.6388888888889, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=30.8724832214765, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310132016, Length=126, Percent_Identity=32.5396825396825, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310110807, Length=126, Percent_Identity=32.5396825396825, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310123363, Length=126, Percent_Identity=32.5396825396825, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=59.8848368522073, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=496, Percent_Identity=29.2338709677419, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI48994988, Length=175, Percent_Identity=36, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=34.5911949685535, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=493, Percent_Identity=27.789046653144, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17556745, Length=180, Percent_Identity=35.5555555555556, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=138, Percent_Identity=37.6811594202899, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=30.4347826086957, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=32.3308270676692, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=32.3308270676692, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=483, Percent_Identity=27.536231884058, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=562, Percent_Identity=24.7330960854093, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324707, Length=159, Percent_Identity=32.0754716981132, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=159, Percent_Identity=32.0754716981132, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=27.8481012658228, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582462, Length=502, Percent_Identity=26.4940239043825, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI221458488, Length=496, Percent_Identity=26.4112903225806, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI78706572, Length=154, Percent_Identity=38.3116883116883, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=30.0653594771242, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=30.0653594771242, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=30.0653594771242, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_DESDG (Q30V54)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_390137.1
- ProteinModelPortal:   Q30V54
- SMR:   Q30V54
- STRING:   Q30V54
- GeneID:   3758636
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_3649
- NMPDR:   fig|207559.3.peg.2534
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- BioCyc:   DDES207559:DDE_3649-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59512; Mature: 59512

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREHQREVDKRRTFGIISHPDAGKTTLTEKLLLFGGAIQMAGTVKSRKAARHATSDWMA
CCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHH
MEQERGISVTTSVMKFHHNGYEINLLDTPGHQDFSEDTYRVLTAVDSALLVIDSAKGVEA
HHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCH
QTLKLMDVCRMRSTPIVTFINKLDRDGLHPLEVMDDIESRLNIQCSPLTWPIGMGADFKG
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCEEECCEEEECCCCCCCCC
TWDIRTGRLHMFTPANDGRIASGRIIEGLDNPELDEALGEQAVTLRDELALLAEAGNPFD
EEEEEECEEEEECCCCCCEEECCCEECCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCC
KERYLKGELTPVFFGSAINNFGVREMLDTFVDLAPAPQPRPATTRTVSPYEEDFSGVVFK
HHHHHCCCCCEEEECHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCEEEE
IQANMDKNHRDRIAFMRICSGKFTRGMKVRHHRIGKEVALANATIFMAQDRTGVEEAWPG
EEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCC
DIIGIHNHGTIKIGDTFSLREPLKFVGIPNFAPEHFRRVVLRDPMKAKQLQKGLEQLAEE
CEEEEECCCEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GAVQLFRPLLGNDYILGAVGVLQFEVIVARLKDEYNVNALYEGCNITTARWLHTDDPKTM
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCHHHH
AEFRDYYRSELAMDAEGCLAYLAPNPWRLESAVERYPKMEFRTTREIS
HHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNREHQREVDKRRTFGIISHPDAGKTTLTEKLLLFGGAIQMAGTVKSRKAARHATSDWMA
CCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHH
MEQERGISVTTSVMKFHHNGYEINLLDTPGHQDFSEDTYRVLTAVDSALLVIDSAKGVEA
HHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCH
QTLKLMDVCRMRSTPIVTFINKLDRDGLHPLEVMDDIESRLNIQCSPLTWPIGMGADFKG
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCEEECCEEEECCCCCCCCC
TWDIRTGRLHMFTPANDGRIASGRIIEGLDNPELDEALGEQAVTLRDELALLAEAGNPFD
EEEEEECEEEEECCCCCCEEECCCEECCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCC
KERYLKGELTPVFFGSAINNFGVREMLDTFVDLAPAPQPRPATTRTVSPYEEDFSGVVFK
HHHHHCCCCCEEEECHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCEEEE
IQANMDKNHRDRIAFMRICSGKFTRGMKVRHHRIGKEVALANATIFMAQDRTGVEEAWPG
EEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCC
DIIGIHNHGTIKIGDTFSLREPLKFVGIPNFAPEHFRRVVLRDPMKAKQLQKGLEQLAEE
CEEEEECCCEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GAVQLFRPLLGNDYILGAVGVLQFEVIVARLKDEYNVNALYEGCNITTARWLHTDDPKTM
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCHHHH
AEFRDYYRSELAMDAEGCLAYLAPNPWRLESAVERYPKMEFRTTREIS
HHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA