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The map label for this gene is kefA [H]

Identifier: 78358486

GI number: 78358486

Start: 3405581

End: 3408031

Strand: Direct

Name: kefA [H]

Synonym: Dde_3447

Alternate gene names: 78358486

Gene position: 3405581-3408031 (Clockwise)

Preceding gene: 78358483

Following gene: 78358487

Centisome position: 91.3

GC content: 57.81

Gene sequence:

>2451_bases
ATGACACCTGCAGCACCCGCAATATCTCAAACTTTCCGGAATATTACAGCCCTATTGATCACCTTTGCCGCCGTGGTGCT
GCTGCACGTCAGCGCCGCACAGGCAGAACAGTCACCGGAAGAGATTGAAAACAGTGAAATACTGCACCTTGTCGAGATAA
AACAACGCGAGCTGAACCTGCTGGAACAGTCGGACCTGAAGCTGCAGGAAGCAATGAAAGGCGCGCTGAAATCGGCATCG
GAAACATACGAAAAAGCGTTGCGCGACTATGAGGAAGCCCAGCTTACGCACGGGCTTTCCGGCGGCACCCCCATGCTTAC
CCGGTCATCCGTACAGCGGTTGAACGCATTATACAACGATCTGGAAACAGCACAGGAGTCTCTTTCCAATGTGCGCGATC
AGGCGCGGGTACGTCTGGAAAGAATAGGCGCTGTTGATGTGGCCCCGCTGCTCAAATCCCGTGACATTTCAGCGGCTACG
CGCAATCTGGTAGACGCCTACCTGCGCGATCTGCGCAACATCCAAAGCAGGCTGGAGACACAGGAGGCCCGTGCCGCCAA
GCTGGAAAGCAGGCTGGGCAAGCTGCGGCAAAACGTACAGGAAAGACGCCTGCAACGCGAAGAAGAGCTGGTTTCAAGCT
GGCGGGATTTCTTTTTCCGCAACAAAGATCCGCTTCTTTCCGTGGAGCTGTGGCGGCAATCCGATTCCGTGGCGTTATGG
GCATCCATGCTGATGACCGGCATGACGCGTGAACTGGGCATGCTGAGCAGCAACGCTTTTTCTGCGGTGCTGTTTGCGCT
GGGCATCATAGGTCTGCTTGCGCTGGGCGGCGTGCCTGTCATCAGGTCGGCTACACGGGGTACCCCCGGCGCCACCCCGC
AGGCCGTGCGGGCGCTGCACACCAGCTGCCTCATAGGCAGTATCGGTCTGGCCGTGGTCATAGCATCGTCGCGCATGTTT
GCCGGAGCGGCCATATATACCAGCGTAGCCGGTATGGTGATGCTCGGCTTTGCCACCATTCGGGCTTCGGCCATTTTGCG
GCGCATGATCAATACCGGATTCATACGGCTGTCACACAGCCCTGCGGCGGTGATGTTTCTGTGCAGCAGCCTGATTGTGG
TCAGCGAGATGCCCGCCAAGCTGGCGGTACCGCTATGGGCCCTGCTGCTGGTTCTGTACGGGTTCTACATGAGCCTGCGC
GACAGGCGGCGCGGTATTCACGGCAGCATAGCCAAAAGATCATGGTTCTGGCTGTGCGCACTGCTGCTCATAGTGGCCGT
GGCAGGCTACGGCGTATTCTCCGCCTTTCTTTTTATGGTGTGGTTTGTTCTGTACGCGGCATACAGCGCCAGCATCGCGC
TCACGGAACTCATCCACCTGCGCGGTGCCGCAAGCACGGCTGATTCCACGGCACTGGTCATGGGCAAAGGGGTTCTTGTG
GGCATTGCCTCTCCGGGGGTGTGGCTGATGAGCTTCGGCGCCGGTCTTCTCTGGCTGTACGGCTTTGTCGGTCAGGGGGT
GTTTGCCAGCATTGCCAATCTTGAACTGAGCTGGGAAGGTTTTTCACTCAAGCTGGCCAGCCTGCTGTTGCTGGCTGCTC
TTTTTTACATCACGCGTGCCTGCACCACACTGGCGCGCGGCGCACTGGCACGCATGTCGCTGCACTGGCCCAGAGGCCAG
CGGGGCGCTGTACTTTCACTGCAGACCACCGCCACATATGCACTCTGGGGCCTGTATGCGCTTATCGCGCTGCATATTCT
GGGGGTAAGCCTGACCAGCCTCACGGTCATTGCCGGTGGCCTCAGCGTCGGTCTGGGGTTCGGCATGCAGACCATTTTCA
ACAACTTCATGAGCGGTCTCATCCTGCTGTTCGGACGGTCCATACAGCAGGGAGACATTATACAAGTGGGCGAACTGTGG
TGCACAGTCAAAAACGTGACCATACGCACCACCATTGTGGAAACATTCGACAGCGCCACCATTCTCATCCCCAACTCGGA
CCTTGTGACCACACAGGTCACCAACTGGACAAAAAACAACACAACCATCCGGCGCGACATCCTTGTGGGAGTGGCCTACG
GTTCTGATACGCGCAAAGTGGAAAAAACCCTGCTGGCACTGGCCAAGGCGCACCCCAATGTCATGCGCCGTCCGGAACCG
CAGGTCATTTTCAGCAACTTCGGCGCCAGCTCGCTGGATTTCTTCCTGCGCATCTGGATAGATGACATCGACAACATGCT
CACCACGGCATCATCGCTGCGCTTTGCCATTGATGAAGAGTTCCGCAAAGAAGAAATAGAAATAGCCTTTCCGCAAATGG
ACCTGCACCTGCGCAGCCTGCCCCCCTCGTGGCAGGTACAACCATCTGCGTCCGTCCGGCAGAAACACAACAGCACTGAA
GCGGAAGACAACATACCGTCTGCGCAGTCCGGCAACGACGCCCCGGCATAG

Upstream 100 bases:

>100_bases
ACCTTGCGTATGCACATGAACCTGTTATGCTGCCTTTCCGCACATTGCCATACCGGCGCGCCGCGCTGCGGCGGAAGCCA
CAAATTACGGAACAGAGCAT

Downstream 100 bases:

>100_bases
GCATGTCCCTGTGTGCAACCCGCAGAGGCGGCACACAGACGCCCGCACAAGCGGCCGGAGTACTGCCGCGCACGGCGGCA
AGGGGGCGTCCGGACTATTT

Product: mechanosensitive ion channel family protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 816; Mature: 815

Protein sequence:

>816_residues
MTPAAPAISQTFRNITALLITFAAVVLLHVSAAQAEQSPEEIENSEILHLVEIKQRELNLLEQSDLKLQEAMKGALKSAS
ETYEKALRDYEEAQLTHGLSGGTPMLTRSSVQRLNALYNDLETAQESLSNVRDQARVRLERIGAVDVAPLLKSRDISAAT
RNLVDAYLRDLRNIQSRLETQEARAAKLESRLGKLRQNVQERRLQREEELVSSWRDFFFRNKDPLLSVELWRQSDSVALW
ASMLMTGMTRELGMLSSNAFSAVLFALGIIGLLALGGVPVIRSATRGTPGATPQAVRALHTSCLIGSIGLAVVIASSRMF
AGAAIYTSVAGMVMLGFATIRASAILRRMINTGFIRLSHSPAAVMFLCSSLIVVSEMPAKLAVPLWALLLVLYGFYMSLR
DRRRGIHGSIAKRSWFWLCALLLIVAVAGYGVFSAFLFMVWFVLYAAYSASIALTELIHLRGAASTADSTALVMGKGVLV
GIASPGVWLMSFGAGLLWLYGFVGQGVFASIANLELSWEGFSLKLASLLLLAALFYITRACTTLARGALARMSLHWPRGQ
RGAVLSLQTTATYALWGLYALIALHILGVSLTSLTVIAGGLSVGLGFGMQTIFNNFMSGLILLFGRSIQQGDIIQVGELW
CTVKNVTIRTTIVETFDSATILIPNSDLVTTQVTNWTKNNTTIRRDILVGVAYGSDTRKVEKTLLALAKAHPNVMRRPEP
QVIFSNFGASSLDFFLRIWIDDIDNMLTTASSLRFAIDEEFRKEEIEIAFPQMDLHLRSLPPSWQVQPSASVRQKHNSTE
AEDNIPSAQSGNDAPA

Sequences:

>Translated_816_residues
MTPAAPAISQTFRNITALLITFAAVVLLHVSAAQAEQSPEEIENSEILHLVEIKQRELNLLEQSDLKLQEAMKGALKSAS
ETYEKALRDYEEAQLTHGLSGGTPMLTRSSVQRLNALYNDLETAQESLSNVRDQARVRLERIGAVDVAPLLKSRDISAAT
RNLVDAYLRDLRNIQSRLETQEARAAKLESRLGKLRQNVQERRLQREEELVSSWRDFFFRNKDPLLSVELWRQSDSVALW
ASMLMTGMTRELGMLSSNAFSAVLFALGIIGLLALGGVPVIRSATRGTPGATPQAVRALHTSCLIGSIGLAVVIASSRMF
AGAAIYTSVAGMVMLGFATIRASAILRRMINTGFIRLSHSPAAVMFLCSSLIVVSEMPAKLAVPLWALLLVLYGFYMSLR
DRRRGIHGSIAKRSWFWLCALLLIVAVAGYGVFSAFLFMVWFVLYAAYSASIALTELIHLRGAASTADSTALVMGKGVLV
GIASPGVWLMSFGAGLLWLYGFVGQGVFASIANLELSWEGFSLKLASLLLLAALFYITRACTTLARGALARMSLHWPRGQ
RGAVLSLQTTATYALWGLYALIALHILGVSLTSLTVIAGGLSVGLGFGMQTIFNNFMSGLILLFGRSIQQGDIIQVGELW
CTVKNVTIRTTIVETFDSATILIPNSDLVTTQVTNWTKNNTTIRRDILVGVAYGSDTRKVEKTLLALAKAHPNVMRRPEP
QVIFSNFGASSLDFFLRIWIDDIDNMLTTASSLRFAIDEEFRKEEIEIAFPQMDLHLRSLPPSWQVQPSASVRQKHNSTE
AEDNIPSAQSGNDAPA
>Mature_815_residues
TPAAPAISQTFRNITALLITFAAVVLLHVSAAQAEQSPEEIENSEILHLVEIKQRELNLLEQSDLKLQEAMKGALKSASE
TYEKALRDYEEAQLTHGLSGGTPMLTRSSVQRLNALYNDLETAQESLSNVRDQARVRLERIGAVDVAPLLKSRDISAATR
NLVDAYLRDLRNIQSRLETQEARAAKLESRLGKLRQNVQERRLQREEELVSSWRDFFFRNKDPLLSVELWRQSDSVALWA
SMLMTGMTRELGMLSSNAFSAVLFALGIIGLLALGGVPVIRSATRGTPGATPQAVRALHTSCLIGSIGLAVVIASSRMFA
GAAIYTSVAGMVMLGFATIRASAILRRMINTGFIRLSHSPAAVMFLCSSLIVVSEMPAKLAVPLWALLLVLYGFYMSLRD
RRRGIHGSIAKRSWFWLCALLLIVAVAGYGVFSAFLFMVWFVLYAAYSASIALTELIHLRGAASTADSTALVMGKGVLVG
IASPGVWLMSFGAGLLWLYGFVGQGVFASIANLELSWEGFSLKLASLLLLAALFYITRACTTLARGALARMSLHWPRGQR
GAVLSLQTTATYALWGLYALIALHILGVSLTSLTVIAGGLSVGLGFGMQTIFNNFMSGLILLFGRSIQQGDIIQVGELWC
TVKNVTIRTTIVETFDSATILIPNSDLVTTQVTNWTKNNTTIRRDILVGVAYGSDTRKVEKTLLALAKAHPNVMRRPEPQ
VIFSNFGASSLDFFLRIWIDDIDNMLTTASSLRFAIDEEFRKEEIEIAFPQMDLHLRSLPPSWQVQPSASVRQKHNSTEA
EDNIPSAQSGNDAPA

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=220, Percent_Identity=42.2727272727273, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI2367355, Length=219, Percent_Identity=27.8538812785388, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1789291, Length=214, Percent_Identity=26.1682242990654, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 89335; Mature: 89204

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAAPAISQTFRNITALLITFAAVVLLHVSAAQAEQSPEEIENSEILHLVEIKQRELNL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEHHHHHHHHHHHH
LEQSDLKLQEAMKGALKSASETYEKALRDYEEAQLTHGLSGGTPMLTRSSVQRLNALYND
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LETAQESLSNVRDQARVRLERIGAVDVAPLLKSRDISAATRNLVDAYLRDLRNIQSRLET
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QEARAAKLESRLGKLRQNVQERRLQREEELVSSWRDFFFRNKDPLLSVELWRQSDSVALW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHEECCCCCHHHHH
ASMLMTGMTRELGMLSSNAFSAVLFALGIIGLLALGGVPVIRSATRGTPGATPQAVRALH
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHH
TSCLIGSIGLAVVIASSRMFAGAAIYTSVAGMVMLGFATIRASAILRRMINTGFIRLSHS
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
PAAVMFLCSSLIVVSEMPAKLAVPLWALLLVLYGFYMSLRDRRRGIHGSIAKRSWFWLCA
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLLIVAVAGYGVFSAFLFMVWFVLYAAYSASIALTELIHLRGAASTADSTALVMGKGVLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEE
GIASPGVWLMSFGAGLLWLYGFVGQGVFASIANLELSWEGFSLKLASLLLLAALFYITRA
EECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHH
CTTLARGALARMSLHWPRGQRGAVLSLQTTATYALWGLYALIALHILGVSLTSLTVIAGG
HHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVGLGFGMQTIFNNFMSGLILLFGRSIQQGDIIQVGELWCTVKNVTIRTTIVETFDSAT
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHEEEECCEEEHHHHHHCCCCE
ILIPNSDLVTTQVTNWTKNNTTIRRDILVGVAYGSDTRKVEKTLLALAKAHPNVMRRPEP
EEECCCCEEEEEECCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHCCCCC
QVIFSNFGASSLDFFLRIWIDDIDNMLTTASSLRFAIDEEFRKEEIEIAFPQMDLHLRSL
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHCC
PPSWQVQPSASVRQKHNSTEAEDNIPSAQSGNDAPA
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPAAPAISQTFRNITALLITFAAVVLLHVSAAQAEQSPEEIENSEILHLVEIKQRELNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEHHHHHHHHHHHH
LEQSDLKLQEAMKGALKSASETYEKALRDYEEAQLTHGLSGGTPMLTRSSVQRLNALYND
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LETAQESLSNVRDQARVRLERIGAVDVAPLLKSRDISAATRNLVDAYLRDLRNIQSRLET
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QEARAAKLESRLGKLRQNVQERRLQREEELVSSWRDFFFRNKDPLLSVELWRQSDSVALW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHEECCCCCHHHHH
ASMLMTGMTRELGMLSSNAFSAVLFALGIIGLLALGGVPVIRSATRGTPGATPQAVRALH
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHH
TSCLIGSIGLAVVIASSRMFAGAAIYTSVAGMVMLGFATIRASAILRRMINTGFIRLSHS
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
PAAVMFLCSSLIVVSEMPAKLAVPLWALLLVLYGFYMSLRDRRRGIHGSIAKRSWFWLCA
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLLIVAVAGYGVFSAFLFMVWFVLYAAYSASIALTELIHLRGAASTADSTALVMGKGVLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEE
GIASPGVWLMSFGAGLLWLYGFVGQGVFASIANLELSWEGFSLKLASLLLLAALFYITRA
EECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHH
CTTLARGALARMSLHWPRGQRGAVLSLQTTATYALWGLYALIALHILGVSLTSLTVIAGG
HHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVGLGFGMQTIFNNFMSGLILLFGRSIQQGDIIQVGELWCTVKNVTIRTTIVETFDSAT
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHEEEECCEEEHHHHHHCCCCE
ILIPNSDLVTTQVTNWTKNNTTIRRDILVGVAYGSDTRKVEKTLLALAKAHPNVMRRPEP
EEECCCCEEEEEECCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHCCCCC
QVIFSNFGASSLDFFLRIWIDDIDNMLTTASSLRFAIDEEFRKEEIEIAFPQMDLHLRSL
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHCC
PPSWQVQPSASVRQKHNSTEAEDNIPSAQSGNDAPA
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]