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The map label for this gene is dosC [H]

Identifier: 78358348

GI number: 78358348

Start: 3294313

End: 3295743

Strand: Direct

Name: dosC [H]

Synonym: Dde_3308

Alternate gene names: 78358348

Gene position: 3294313-3295743 (Clockwise)

Preceding gene: 78358347

Following gene: 78358351

Centisome position: 88.31

GC content: 57.44

Gene sequence:

>1431_bases
ATGCAGCTCAAAACACGTCTCATCTCCGCACTGGCGGCCATTCTCATTACCGTTTTTCTGGCGACAAGCATCATCAACTA
TGTTGTCACCCGCAATGCAGTGCGTGACGATCTGCTCCGCTCCGCCCTGCCGCTGACCGGCAAAAACATTTATTCCGAAA
TTCACAGTGCGCTCATGCGTCCCCTTCTGGTGTCTTCGGCCATGGCCAACGACACCTTTTTGCGCGACTGGGTGACCGGA
GGCGAACAGAATGAGGACGCAGTGCGCCGGTATCTTTCCAGAATCAAAAAGGAATATGGCTTTTTAAGCACCTTTTTCGT
TTCGGCGGTAACCGACCGCTATTACACACCGGACGGCATTCTGAAAAAAATCGGTCCGCGCGACCCGCATGATGTGTGGT
TCTACGCATTCACACGGTACAAAGAGCCATACGACCTTGATATCGACGTCAATCAGGCTGCCGGCAACACCATGACCGTG
TTTGTAAACTTCCGCCTTGAAGACGACCGCGGCAGGCTGCTGGGCGTTACCGGGGCAGGCATAGCGCTGGATAAGGCTCT
GGAGCTGCTGGATGAAGCACAGCGCACGTATCTGCGTCAGGCCTTCCTCATCGATCAGGACGGGTTGATACAGATACATC
CCGACACGTCACTGGTGGAAAAAGCCTACATAACCGAAATGGACGGGCTGCGTGACATTGCGCCGGACATACTGGCAGGT
CAGGGCGATGCAGTCAGCCTGCAGTACGACGCCGGCGGCAGACATTATCTGCTTACGTCGCGCTATATCCCTGAATTCAA
ATGGCATCTGCTGGTGGTGCAGGATGAAATGACAGCCCTGCGCACGGCCAGAGGCAACTTCATGCGCACGCTGGCCATGG
GGTTTGTCTCCACACTTGTCATACTGGCCCTGTGCGTGCTGGCGGTTAACAAATATCAGGCACGCATAGAGCATCTTGCC
AGAACAGACCCGCTGACAGGAGCAGCCAACCGCCGCGAGCTGGAGGAACGCTTTGACCTGGCCGCATACAGGGCATCGCG
CGACGGAATCCCCTTCTCCATACTGATCATCGATCTGGACGGTTTCAAGGAAGTAAACGACAGGCTGGGGCATCTTGCCG
GTGACGAAGTACTGGCATCCATGGCCCGCGTCATACGCGGCAGGCTGCGCCCCACCGATCTTCTGGCCCGCTGGGGCGGA
GACGAATTCATCATCCTGCTGGACGGAAATGAAGAAAGCGCCCGGGCGCTGGCACGGCGCATATCCGCCGCAGTATGCGC
CGCTCCCGACATTCCTGTGGGGTTCAGTTCCGGTGTGGCGGCCTACCGCGACAGCGACGACCTGACCTCCCTGACCCGAA
GGGCCGACAACGCCATGTATCAGGCAAAAGCACGGGGCGGCGACTGCGTCATGGCAGCGGAAGATTTGTAA

Upstream 100 bases:

>100_bases
GCTGCCGCGGGCAGACCCCTGTCAGGCGCTGTTTCCGGCGCAGGCACACGTATCATCATCCTCTGCACCCCTACAGCGGC
TCCGCGTCGCGGAACAGACA

Downstream 100 bases:

>100_bases
ACAGGGCTGACATGTGCCGCCCGTCACGGGCAGAGGATGCAGGCGGACACAACCGGATGCCGTCCATCCCGGTCTGCCGG
CAGAGACCGCCGGCCGCAGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MQLKTRLISALAAILITVFLATSIINYVVTRNAVRDDLLRSALPLTGKNIYSEIHSALMRPLLVSSAMANDTFLRDWVTG
GEQNEDAVRRYLSRIKKEYGFLSTFFVSAVTDRYYTPDGILKKIGPRDPHDVWFYAFTRYKEPYDLDIDVNQAAGNTMTV
FVNFRLEDDRGRLLGVTGAGIALDKALELLDEAQRTYLRQAFLIDQDGLIQIHPDTSLVEKAYITEMDGLRDIAPDILAG
QGDAVSLQYDAGGRHYLLTSRYIPEFKWHLLVVQDEMTALRTARGNFMRTLAMGFVSTLVILALCVLAVNKYQARIEHLA
RTDPLTGAANRRELEERFDLAAYRASRDGIPFSILIIDLDGFKEVNDRLGHLAGDEVLASMARVIRGRLRPTDLLARWGG
DEFIILLDGNEESARALARRISAAVCAAPDIPVGFSSGVAAYRDSDDLTSLTRRADNAMYQAKARGGDCVMAAEDL

Sequences:

>Translated_476_residues
MQLKTRLISALAAILITVFLATSIINYVVTRNAVRDDLLRSALPLTGKNIYSEIHSALMRPLLVSSAMANDTFLRDWVTG
GEQNEDAVRRYLSRIKKEYGFLSTFFVSAVTDRYYTPDGILKKIGPRDPHDVWFYAFTRYKEPYDLDIDVNQAAGNTMTV
FVNFRLEDDRGRLLGVTGAGIALDKALELLDEAQRTYLRQAFLIDQDGLIQIHPDTSLVEKAYITEMDGLRDIAPDILAG
QGDAVSLQYDAGGRHYLLTSRYIPEFKWHLLVVQDEMTALRTARGNFMRTLAMGFVSTLVILALCVLAVNKYQARIEHLA
RTDPLTGAANRRELEERFDLAAYRASRDGIPFSILIIDLDGFKEVNDRLGHLAGDEVLASMARVIRGRLRPTDLLARWGG
DEFIILLDGNEESARALARRISAAVCAAPDIPVGFSSGVAAYRDSDDLTSLTRRADNAMYQAKARGGDCVMAAEDL
>Mature_476_residues
MQLKTRLISALAAILITVFLATSIINYVVTRNAVRDDLLRSALPLTGKNIYSEIHSALMRPLLVSSAMANDTFLRDWVTG
GEQNEDAVRRYLSRIKKEYGFLSTFFVSAVTDRYYTPDGILKKIGPRDPHDVWFYAFTRYKEPYDLDIDVNQAAGNTMTV
FVNFRLEDDRGRLLGVTGAGIALDKALELLDEAQRTYLRQAFLIDQDGLIQIHPDTSLVEKAYITEMDGLRDIAPDILAG
QGDAVSLQYDAGGRHYLLTSRYIPEFKWHLLVVQDEMTALRTARGNFMRTLAMGFVSTLVILALCVLAVNKYQARIEHLA
RTDPLTGAANRRELEERFDLAAYRASRDGIPFSILIIDLDGFKEVNDRLGHLAGDEVLASMARVIRGRLRPTDLLARWGG
DEFIILLDGNEESARALARRISAAVCAAPDIPVGFSSGVAAYRDSDDLTSLTRRADNAMYQAKARGGDCVMAAEDL

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=256, Percent_Identity=31.25, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=35, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787262, Length=188, Percent_Identity=34.5744680851064, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=35.8381502890173, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081974, Length=181, Percent_Identity=30.939226519337, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082007, Length=201, Percent_Identity=31.8407960199005, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=37.5, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=32.3353293413174, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=33.5403726708075, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=29.7619047619048, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787802, Length=179, Percent_Identity=27.9329608938547, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI87081977, Length=284, Percent_Identity=26.7605633802817, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 53027; Mature: 53027

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLKTRLISALAAILITVFLATSIINYVVTRNAVRDDLLRSALPLTGKNIYSEIHSALMR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
PLLVSSAMANDTFLRDWVTGGEQNEDAVRRYLSRIKKEYGFLSTFFVSAVTDRYYTPDGI
HHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LKKIGPRDPHDVWFYAFTRYKEPYDLDIDVNQAAGNTMTVFVNFRLEDDRGRLLGVTGAG
HHHCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCEEEEEEEEEEECCCCCEEEECCCC
IALDKALELLDEAQRTYLRQAFLIDQDGLIQIHPDTSLVEKAYITEMDGLRDIAPDILAG
HHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCHHHHHHHHHHHHCCHHHHCHHHHCC
QGDAVSLQYDAGGRHYLLTSRYIPEFKWHLLVVQDEMTALRTARGNFMRTLAMGFVSTLV
CCCEEEEEECCCCCEEEEECCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
ILALCVLAVNKYQARIEHLARTDPLTGAANRRELEERFDLAAYRASRDGIPFSILIIDLD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
GFKEVNDRLGHLAGDEVLASMARVIRGRLRPTDLLARWGGDEFIILLDGNEESARALARR
CHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
ISAAVCAAPDIPVGFSSGVAAYRDSDDLTSLTRRADNAMYQAKARGGDCVMAAEDL
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure
MQLKTRLISALAAILITVFLATSIINYVVTRNAVRDDLLRSALPLTGKNIYSEIHSALMR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
PLLVSSAMANDTFLRDWVTGGEQNEDAVRRYLSRIKKEYGFLSTFFVSAVTDRYYTPDGI
HHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LKKIGPRDPHDVWFYAFTRYKEPYDLDIDVNQAAGNTMTVFVNFRLEDDRGRLLGVTGAG
HHHCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCEEEEEEEEEEECCCCCEEEECCCC
IALDKALELLDEAQRTYLRQAFLIDQDGLIQIHPDTSLVEKAYITEMDGLRDIAPDILAG
HHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCHHHHHHHHHHHHCCHHHHCHHHHCC
QGDAVSLQYDAGGRHYLLTSRYIPEFKWHLLVVQDEMTALRTARGNFMRTLAMGFVSTLV
CCCEEEEEECCCCCEEEEECCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
ILALCVLAVNKYQARIEHLARTDPLTGAANRRELEERFDLAAYRASRDGIPFSILIIDLD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
GFKEVNDRLGHLAGDEVLASMARVIRGRLRPTDLLARWGGDEFIILLDGNEESARALARR
CHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
ISAAVCAAPDIPVGFSSGVAAYRDSDDLTSLTRRADNAMYQAKARGGDCVMAAEDL
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]