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The map label for this gene is glcD [H]

Identifier: 78358279

GI number: 78358279

Start: 3232872

End: 3234254

Strand: Direct

Name: glcD [H]

Synonym: Dde_3239

Alternate gene names: 78358279

Gene position: 3232872-3234254 (Clockwise)

Preceding gene: 78358278

Following gene: 78358280

Centisome position: 86.67

GC content: 59.44

Gene sequence:

>1383_bases
ATGCTCAGTGAATCCCTTATCAAGGAATTTAAAACCATCGTCGGTGACGAGAACGTTTTCGACAACGAAGCAGACCGCCA
GTGCTATTCGTACGACTCTGCCGTGCTTGACGCCGTTGTCCCTGCACTGGTTGTGCGCCCGACCGAGACCACTCAGATCG
GTCAGGTGGTCAAGCTGTGCAACGAAAACGGTCTGCCCATCACCGTGCGCGGCGCAGGTACCAACCTGTCCGGCGGCACC
ATTCCCGACCCGCGCGACGGCGTGGTTGTACTGACCAACGGCCTGACCAAAATTCTGGAAATCAACGAGCAGGACCTGTA
CGCCGTTGTTCAGCCCGGTGTGGTCACCGCCAAGTTTGCTGCCGAAGTGGCCAAACGCGGCCTTTTCTACCCGCCCGACC
CGGGTTCGCAGGCTGTTTCCACCATCGCCGGTAACGTGGCGGAAAATGCCGGCGGTCTGCGCGGTCTGAAGTACGGCGTG
ACCAAAGACTACGTCATGGGCATCGAATTCTACGATGTAAACGGCGGTCTGGTGAAAACCGGTTCCCGCACTGTAAAGTG
CGTCACCGGCTACAACCTTGCGGGCCTGATGGCTGCCTCCGAAGGCACCCTTGGCGTGTTCAGCGAAATCATTCTGAAGC
TGGTTCCGCCGCCACGCGCCTCCAAGGCCATGATGGCTGTTTTCGACGACATCAACAAAGCATCCGAAGCCGTTGCCGGC
ATCATTGCCGCGCACGTTGTGCCCTGCACCCTTGAGTTCATGGACCAGGCCAGCATCCGCTACGTGGAAGACTACACCAA
GGCCGGACTGCCCGTACACGCCGAAGCCATCCTGCTCATCGAGGTGGACGGTCACCCCGGTCAGGTGGAAGACGACGCCG
CCCGCGTGGAACAGGTGCTCAAGAGCACCGGTGCCGAAATTCAGGTGGCCAAGGACGCCGAAGAAAAGTTCAAGCTGTGG
GAAGCACGCCGCAATGCGCTGCCCGCCCTCGCCCGTGCCAAACCGACCACCGTACTCGAAGACGCCACCGTGCCGCGGTC
GCAGATTCCTGCCATGGTCAAGGCCATCAACGACATCGCCGCCAAGTACCAGATCAACATCGGTACCTTCGGCCATGCCG
GCGACGGCAACCTGCACCCCACCATTCTGTGCGACCGCAGAGACAAAAAGGAATTCGAACGCGTGGAACACGCCGTGGAC
GAAATCTTCGACGTGGCACTGTCGCTCAAGGGTACGCTGTCCGGCGAACACGGCATCGGCATGGCCAAGTCCAAATGGAT
GGAAAAGGAAACTTCCAGAGCCACCATTGACTTCTCGCTGAACATGAAGCGGGCCATCGACCCCAACAACATTCTCAATC
CCGGCAAGATCATCGGAGGCTAA

Upstream 100 bases:

>100_bases
CGGAGCGAACTACGGTACCTGCTCCGTTTTTTGTCTCACAGGGCGCGGGTCACGCCGCCCCGGTGCAGAGGAAGAAACCC
CTTTCGGAGGAAGCATCTCC

Downstream 100 bases:

>100_bases
TGCAATGGCCGATCTGAATAAACTTGCCAAAATGCTGCAGGAACTGGACGACCAGATGGTCGCCTGCATGAAATGCGGCA
TGTGTCAGGCTGTGTGTCCC

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLSESLIKEFKTIVGDENVFDNEADRQCYSYDSAVLDAVVPALVVRPTETTQIGQVVKLCNENGLPITVRGAGTNLSGGT
IPDPRDGVVVLTNGLTKILEINEQDLYAVVQPGVVTAKFAAEVAKRGLFYPPDPGSQAVSTIAGNVAENAGGLRGLKYGV
TKDYVMGIEFYDVNGGLVKTGSRTVKCVTGYNLAGLMAASEGTLGVFSEIILKLVPPPRASKAMMAVFDDINKASEAVAG
IIAAHVVPCTLEFMDQASIRYVEDYTKAGLPVHAEAILLIEVDGHPGQVEDDAARVEQVLKSTGAEIQVAKDAEEKFKLW
EARRNALPALARAKPTTVLEDATVPRSQIPAMVKAINDIAAKYQINIGTFGHAGDGNLHPTILCDRRDKKEFERVEHAVD
EIFDVALSLKGTLSGEHGIGMAKSKWMEKETSRATIDFSLNMKRAIDPNNILNPGKIIGG

Sequences:

>Translated_460_residues
MLSESLIKEFKTIVGDENVFDNEADRQCYSYDSAVLDAVVPALVVRPTETTQIGQVVKLCNENGLPITVRGAGTNLSGGT
IPDPRDGVVVLTNGLTKILEINEQDLYAVVQPGVVTAKFAAEVAKRGLFYPPDPGSQAVSTIAGNVAENAGGLRGLKYGV
TKDYVMGIEFYDVNGGLVKTGSRTVKCVTGYNLAGLMAASEGTLGVFSEIILKLVPPPRASKAMMAVFDDINKASEAVAG
IIAAHVVPCTLEFMDQASIRYVEDYTKAGLPVHAEAILLIEVDGHPGQVEDDAARVEQVLKSTGAEIQVAKDAEEKFKLW
EARRNALPALARAKPTTVLEDATVPRSQIPAMVKAINDIAAKYQINIGTFGHAGDGNLHPTILCDRRDKKEFERVEHAVD
EIFDVALSLKGTLSGEHGIGMAKSKWMEKETSRATIDFSLNMKRAIDPNNILNPGKIIGG
>Mature_460_residues
MLSESLIKEFKTIVGDENVFDNEADRQCYSYDSAVLDAVVPALVVRPTETTQIGQVVKLCNENGLPITVRGAGTNLSGGT
IPDPRDGVVVLTNGLTKILEINEQDLYAVVQPGVVTAKFAAEVAKRGLFYPPDPGSQAVSTIAGNVAENAGGLRGLKYGV
TKDYVMGIEFYDVNGGLVKTGSRTVKCVTGYNLAGLMAASEGTLGVFSEIILKLVPPPRASKAMMAVFDDINKASEAVAG
IIAAHVVPCTLEFMDQASIRYVEDYTKAGLPVHAEAILLIEVDGHPGQVEDDAARVEQVLKSTGAEIQVAKDAEEKFKLW
EARRNALPALARAKPTTVLEDATVPRSQIPAMVKAINDIAAKYQINIGTFGHAGDGNLHPTILCDRRDKKEFERVEHAVD
EIFDVALSLKGTLSGEHGIGMAKSKWMEKETSRATIDFSLNMKRAIDPNNILNPGKIIGG

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=463, Percent_Identity=31.9654427645788, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI37595754, Length=486, Percent_Identity=30.6584362139918, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI119964728, Length=432, Percent_Identity=25.9259259259259, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI4501993, Length=225, Percent_Identity=24.8888888888889, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1789351, Length=419, Percent_Identity=40.0954653937947, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1787977, Length=518, Percent_Identity=22.3938223938224, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71992373, Length=462, Percent_Identity=30.0865800865801, Blast_Score=227, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI71992381, Length=462, Percent_Identity=29.004329004329, Blast_Score=214, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI71992392, Length=379, Percent_Identity=29.8153034300792, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17534361, Length=427, Percent_Identity=25.7611241217799, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17556096, Length=219, Percent_Identity=25.5707762557078, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320027, Length=433, Percent_Identity=29.7921478060046, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6320023, Length=482, Percent_Identity=26.9709543568465, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6320764, Length=475, Percent_Identity=26.7368421052632, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI18921117, Length=474, Percent_Identity=27.2151898734177, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24639277, Length=474, Percent_Identity=27.2151898734177, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24639275, Length=474, Percent_Identity=27.2151898734177, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24653753, Length=226, Percent_Identity=26.1061946902655, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 49289; Mature: 49289

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSESLIKEFKTIVGDENVFDNEADRQCYSYDSAVLDAVVPALVVRPTETTQIGQVVKLC
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
NENGLPITVRGAGTNLSGGTIPDPRDGVVVLTNGLTKILEINEQDLYAVVQPGVVTAKFA
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCEEHHCCCCCCEEEEECCCCCHHHHH
AEVAKRGLFYPPDPGSQAVSTIAGNVAENAGGLRGLKYGVTKDYVMGIEFYDVNGGLVKT
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEEEEECCCCEEEC
GSRTVKCVTGYNLAGLMAASEGTLGVFSEIILKLVPPPRASKAMMAVFDDINKASEAVAG
CCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
IIAAHVVPCTLEFMDQASIRYVEDYTKAGLPVHAEAILLIEVDGHPGQVEDDAARVEQVL
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHH
KSTGAEIQVAKDAEEKFKLWEARRNALPALARAKPTTVLEDATVPRSQIPAMVKAINDIA
HHCCCEEEEECCHHHHHHHHHHHHHCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHH
AKYQINIGTFGHAGDGNLHPTILCDRRDKKEFERVEHAVDEIFDVALSLKGTLSGEHGIG
HEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
MAKSKWMEKETSRATIDFSLNMKRAIDPNNILNPGKIIGG
CHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLSESLIKEFKTIVGDENVFDNEADRQCYSYDSAVLDAVVPALVVRPTETTQIGQVVKLC
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
NENGLPITVRGAGTNLSGGTIPDPRDGVVVLTNGLTKILEINEQDLYAVVQPGVVTAKFA
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCEEHHCCCCCCEEEEECCCCCHHHHH
AEVAKRGLFYPPDPGSQAVSTIAGNVAENAGGLRGLKYGVTKDYVMGIEFYDVNGGLVKT
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEEEEECCCCEEEC
GSRTVKCVTGYNLAGLMAASEGTLGVFSEIILKLVPPPRASKAMMAVFDDINKASEAVAG
CCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
IIAAHVVPCTLEFMDQASIRYVEDYTKAGLPVHAEAILLIEVDGHPGQVEDDAARVEQVL
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHH
KSTGAEIQVAKDAEEKFKLWEARRNALPALARAKPTTVLEDATVPRSQIPAMVKAINDIA
HHCCCEEEEECCHHHHHHHHHHHHHCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHH
AKYQINIGTFGHAGDGNLHPTILCDRRDKKEFERVEHAVDEIFDVALSLKGTLSGEHGIG
HEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
MAKSKWMEKETSRATIDFSLNMKRAIDPNNILNPGKIIGG
CHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]