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The map label for this gene is pspF [H]

Identifier: 78358267

GI number: 78358267

Start: 3215833

End: 3216921

Strand: Direct

Name: pspF [H]

Synonym: Dde_3227

Alternate gene names: 78358267

Gene position: 3215833-3216921 (Clockwise)

Preceding gene: 78358263

Following gene: 78358268

Centisome position: 86.21

GC content: 62.35

Gene sequence:

>1089_bases
ATGAGCAGGCGTACACAGCCACATGCCGCCCACGTGGAAGCGCTGGGCAGTTCCGATGTGTTTCTGGCATTTCAGGAACA
TGTATCCCGTGCGGCAAAGGTGGAAAGGCCCGTGCTGCTGCTGGGCGAACGCGGCACCGGCAAGGAACTGGCCGCCACCC
GGCTGCACTACCTTTCATCGCGCTGGCAGGGGCCGCTGGTCACATTGAACTGTGCGGCCATATCACCTGCCCTCATGGAA
TCGGAACTCTTCGGCCACGAAAGCGGTGCCTTCACCGGCGCCGAAAGACAGCGCCGGGGCAGGTTCGAGCAGGCGCACGG
CGGCACCCTGTTTCTGGACGAGATAGGCAACATGCCGCCGGCCATGCAGGAAAAAATTCTGCGCGTTGTCGAATACGGCG
TTTTCGAGCGCGTCGGCGGTTCACACGCCGTGGAGACAGACGTGCGCATAGTGGCCGCCACCAATGTTGATCTGGCGCGC
ATGGCCGATGCAGGCCGGTTCAAACGGGATCTGCTGGACAGGCTGGCATTTGAGGTGCTCACGCTGCCCCCGCTGCGGGA
ACGGGGTGAAGACATTATGCTGCTGGCACGGCGTTTTGCCGCTTCCATGGCCGCGGAACTGGGCAGACCGGAAACGCCGC
GGTTTACCCCGCACGCCGAAGCCGCCCTGAAAAATCACAGCTGGCCCGGCAACGTGCGCGAACTGAAAAACACAGTCGAG
CGCGCCGTGTTTCACTCTGACGGCGCTCTCATAGATACCATAGAAACAGACCCTTTTGTCTCTCCCTGGCGGCAGGGGCT
TGCGGCACCATATCCGGAACACCACGGCACAGAAAATCCGGACCCTCCTTCACAACGTCCGCAGGCGCCCTGCCCGCAAG
GGGACACCGCGCCCTTTCCTTCTTCCGTGCCCGATCTTTCCATACCGCTGCAGCGGGCCGCCCATGCACTGGAGCAGGAA
TACCTGCGCGCCGCGCTGGAACGCACACGCCACAATCAGCGGCAGGCCGCAGAGCTGCTGGGCCTGACCTACCACCAGTT
TCGCGGGTTGTTCCGCAAACATAAACAGGATATTGTCGCTGACCCGTAA

Upstream 100 bases:

>100_bases
ATTCTGTTTTTACAGTTTATTTTATATCGGGCACGAACACGCGATGTGGTATACAAAACCATTTAGTAGCAATTTAAACC
ATATTATGGCATAAAAAACC

Downstream 100 bases:

>100_bases
GGATGTAAACACGCACACAACAGCACACAAGCGAGTAATGCCATGTCTTCCTTCAAGCGCAATGTCTACCTGAAGACAGT
TCCCATACCGCAGGCTGTGG

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSRRTQPHAAHVEALGSSDVFLAFQEHVSRAAKVERPVLLLGERGTGKELAATRLHYLSSRWQGPLVTLNCAAISPALME
SELFGHESGAFTGAERQRRGRFEQAHGGTLFLDEIGNMPPAMQEKILRVVEYGVFERVGGSHAVETDVRIVAATNVDLAR
MADAGRFKRDLLDRLAFEVLTLPPLRERGEDIMLLARRFAASMAAELGRPETPRFTPHAEAALKNHSWPGNVRELKNTVE
RAVFHSDGALIDTIETDPFVSPWRQGLAAPYPEHHGTENPDPPSQRPQAPCPQGDTAPFPSSVPDLSIPLQRAAHALEQE
YLRAALERTRHNQRQAAELLGLTYHQFRGLFRKHKQDIVADP

Sequences:

>Translated_362_residues
MSRRTQPHAAHVEALGSSDVFLAFQEHVSRAAKVERPVLLLGERGTGKELAATRLHYLSSRWQGPLVTLNCAAISPALME
SELFGHESGAFTGAERQRRGRFEQAHGGTLFLDEIGNMPPAMQEKILRVVEYGVFERVGGSHAVETDVRIVAATNVDLAR
MADAGRFKRDLLDRLAFEVLTLPPLRERGEDIMLLARRFAASMAAELGRPETPRFTPHAEAALKNHSWPGNVRELKNTVE
RAVFHSDGALIDTIETDPFVSPWRQGLAAPYPEHHGTENPDPPSQRPQAPCPQGDTAPFPSSVPDLSIPLQRAAHALEQE
YLRAALERTRHNQRQAAELLGLTYHQFRGLFRKHKQDIVADP
>Mature_361_residues
SRRTQPHAAHVEALGSSDVFLAFQEHVSRAAKVERPVLLLGERGTGKELAATRLHYLSSRWQGPLVTLNCAAISPALMES
ELFGHESGAFTGAERQRRGRFEQAHGGTLFLDEIGNMPPAMQEKILRVVEYGVFERVGGSHAVETDVRIVAATNVDLARM
ADAGRFKRDLLDRLAFEVLTLPPLRERGEDIMLLARRFAASMAAELGRPETPRFTPHAEAALKNHSWPGNVRELKNTVER
AVFHSDGALIDTIETDPFVSPWRQGLAAPYPEHHGTENPDPPSQRPQAPCPQGDTAPFPSSVPDLSIPLQRAAHALEQEY
LRAALERTRHNQRQAAELLGLTYHQFRGLFRKHKQDIVADP

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=344, Percent_Identity=50.2906976744186, Blast_Score=331, Evalue=5e-92,
Organism=Escherichia coli, GI1788550, Length=329, Percent_Identity=39.5136778115502, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790437, Length=335, Percent_Identity=40.2985074626866, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1789087, Length=239, Percent_Identity=47.6987447698745, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87082117, Length=240, Percent_Identity=48.75, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1790299, Length=331, Percent_Identity=42.9003021148036, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87082152, Length=330, Percent_Identity=38.1818181818182, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789233, Length=354, Percent_Identity=36.7231638418079, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1786524, Length=288, Percent_Identity=40.9722222222222, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1788905, Length=340, Percent_Identity=35.5882352941176, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1787583, Length=223, Percent_Identity=43.4977578475336, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=36.0169491525424, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87081858, Length=231, Percent_Identity=34.6320346320346, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 40200; Mature: 40069

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRTQPHAAHVEALGSSDVFLAFQEHVSRAAKVERPVLLLGERGTGKELAATRLHYLSS
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
RWQGPLVTLNCAAISPALMESELFGHESGAFTGAERQRRGRFEQAHGGTLFLDEIGNMPP
CCCCCEEEEEHHHHCHHHHHHHHHCCCCCCCCCHHHHHHCCCHHCCCCEEEHHHHCCCCH
AMQEKILRVVEYGVFERVGGSHAVETDVRIVAATNVDLARMADAGRFKRDLLDRLAFEVL
HHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
TLPPLRERGEDIMLLARRFAASMAAELGRPETPRFTPHAEAALKNHSWPGNVRELKNTVE
CCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHH
RAVFHSDGALIDTIETDPFVSPWRQGLAAPYPEHHGTENPDPPSQRPQAPCPQGDTAPFP
HHHHHCCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SSVPDLSIPLQRAAHALEQEYLRAALERTRHNQRQAAELLGLTYHQFRGLFRKHKQDIVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DP
CC
>Mature Secondary Structure 
SRRTQPHAAHVEALGSSDVFLAFQEHVSRAAKVERPVLLLGERGTGKELAATRLHYLSS
CCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
RWQGPLVTLNCAAISPALMESELFGHESGAFTGAERQRRGRFEQAHGGTLFLDEIGNMPP
CCCCCEEEEEHHHHCHHHHHHHHHCCCCCCCCCHHHHHHCCCHHCCCCEEEHHHHCCCCH
AMQEKILRVVEYGVFERVGGSHAVETDVRIVAATNVDLARMADAGRFKRDLLDRLAFEVL
HHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
TLPPLRERGEDIMLLARRFAASMAAELGRPETPRFTPHAEAALKNHSWPGNVRELKNTVE
CCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHH
RAVFHSDGALIDTIETDPFVSPWRQGLAAPYPEHHGTENPDPPSQRPQAPCPQGDTAPFP
HHHHHCCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SSVPDLSIPLQRAAHALEQEYLRAALERTRHNQRQAAELLGLTYHQFRGLFRKHKQDIVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]