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The map label for this gene is leuB

Identifier: 78358262

GI number: 78358262

Start: 3211979

End: 3213055

Strand: Direct

Name: leuB

Synonym: Dde_3222

Alternate gene names: 78358262

Gene position: 3211979-3213055 (Clockwise)

Preceding gene: 78358261

Following gene: 78358263

Centisome position: 86.11

GC content: 61.65

Gene sequence:

>1077_bases
ATGGAAATGCGCATATGCCTGCTTGCCGGTGACGGCATAGGGCCGGAGATAATGGAACAGGGCGTGCGGGTGCTGGAAAC
CGTGGCCGGAAAATTCGGCCACGACATGGAATTTACCGATGCGCTTATCGGCGGAGCGGCCATTGACGAAACAGGCGATC
CCCTGCCGCAGGAAACCGTGGAAGCCTGCAAAGCGGCCCAAGCCGTGCTGCTGGGTGCTGTGGGCGGCCCCCGCTGGGAC
AACCTGACCGGCGCCGAACGACCCGAAGCCGGTCTGCTCGGCATCCGCAAAGCTCTCGGCCTGTTTGCCAACCTGCGCCC
CGCAAAACTTTTTCCCGAACTGGCAAGCGCCTGCTTTCTGCGGCCTGACATAGTTGCCAACGGCATTGATGTCATGGTGG
TACGCGAACTGACCGGCGGCATTTATTTCGGCACTCCGGCCGGTGAAGAAACCCGTGACGGCCTGCGGACCGCCTTTAAC
ACCATGGTGTACAACGAAGAAGAAGTCCGCCGCATCGCCCGCGTGGCTTTTGAAGCCGCCCGCAAGCGCTCCGGCAGGGT
CTGCTCGGTGGACAAGGCCAACGTGCTGGCCGTGTCGCGTCTGTGGCGCGAGATAGTCACAGCCACCCACGCCGACGAAT
TCCCCGACGTGGAGCTTTCGCACATGTATGTTGACAACGCGGCCATGCAGCTGGTGCGCGACCCATCGCAGTTTGACGTC
ATCGTGACCGGCAACCTGTTCGGCGACATTCTTTCGGATGAAGCTTCGGTCATCACCGGTTCCATTGGCATGCTGCCATC
GGCTTCGCTGGGTGCCGGAGGGCCCGGACTGTTCGAACCCATCCACGGCTCCGCCCCCGACATCGCCGGGCAGAACAAAG
CCAACCCGCTGGCTACCATTCTTTCCACAGCCATGATGCTGCGCCACAGCTTCGGGCTGGAAAAAGAAGCCGAAGCCGTC
GAAGGCGCCGTGCAACAGGTACTCAAAGCCGGCTACCGCACCGGTGACATAATGGATCAGGGCGGCACGCTGGTGGGATG
CAACACCATGGGCAACCTTGTCAACGAACGCATCTGA

Upstream 100 bases:

>100_bases
GGCACAGATGCTGGACGTGCCGCAGGGCACCTCGATGTTTTTGCTTCAACAACCGTAAGCCGGAACTCTCCGGTACGGAC
AGGGTCAAGGAGACCATATC

Downstream 100 bases:

>100_bases
CGACCCGCAGCCCGTGTCCCGCCAACGGGGCACGGGCGCTATGGACTTGGTGGGGCAGTGAGTGTACCATCGGGCCGACA
CCGGAGGTATCTATGCGAGC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MEMRICLLAGDGIGPEIMEQGVRVLETVAGKFGHDMEFTDALIGGAAIDETGDPLPQETVEACKAAQAVLLGAVGGPRWD
NLTGAERPEAGLLGIRKALGLFANLRPAKLFPELASACFLRPDIVANGIDVMVVRELTGGIYFGTPAGEETRDGLRTAFN
TMVYNEEEVRRIARVAFEAARKRSGRVCSVDKANVLAVSRLWREIVTATHADEFPDVELSHMYVDNAAMQLVRDPSQFDV
IVTGNLFGDILSDEASVITGSIGMLPSASLGAGGPGLFEPIHGSAPDIAGQNKANPLATILSTAMMLRHSFGLEKEAEAV
EGAVQQVLKAGYRTGDIMDQGGTLVGCNTMGNLVNERI

Sequences:

>Translated_358_residues
MEMRICLLAGDGIGPEIMEQGVRVLETVAGKFGHDMEFTDALIGGAAIDETGDPLPQETVEACKAAQAVLLGAVGGPRWD
NLTGAERPEAGLLGIRKALGLFANLRPAKLFPELASACFLRPDIVANGIDVMVVRELTGGIYFGTPAGEETRDGLRTAFN
TMVYNEEEVRRIARVAFEAARKRSGRVCSVDKANVLAVSRLWREIVTATHADEFPDVELSHMYVDNAAMQLVRDPSQFDV
IVTGNLFGDILSDEASVITGSIGMLPSASLGAGGPGLFEPIHGSAPDIAGQNKANPLATILSTAMMLRHSFGLEKEAEAV
EGAVQQVLKAGYRTGDIMDQGGTLVGCNTMGNLVNERI
>Mature_358_residues
MEMRICLLAGDGIGPEIMEQGVRVLETVAGKFGHDMEFTDALIGGAAIDETGDPLPQETVEACKAAQAVLLGAVGGPRWD
NLTGAERPEAGLLGIRKALGLFANLRPAKLFPELASACFLRPDIVANGIDVMVVRELTGGIYFGTPAGEETRDGLRTAFN
TMVYNEEEVRRIARVAFEAARKRSGRVCSVDKANVLAVSRLWREIVTATHADEFPDVELSHMYVDNAAMQLVRDPSQFDV
IVTGNLFGDILSDEASVITGSIGMLPSASLGAGGPGLFEPIHGSAPDIAGQNKANPLATILSTAMMLRHSFGLEKEAEAV
EGAVQQVLKAGYRTGDIMDQGGTLVGCNTMGNLVNERI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=33.4328358208955, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI28178821, Length=323, Percent_Identity=28.4829721362229, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178816, Length=323, Percent_Identity=28.7925696594427, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4758582, Length=350, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI28178838, Length=330, Percent_Identity=28.4848484848485, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI28178819, Length=157, Percent_Identity=36.3057324840764, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=55.6818181818182, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1788101, Length=352, Percent_Identity=36.9318181818182, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1787381, Length=402, Percent_Identity=25.3731343283582, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=32.7485380116959, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI25144293, Length=342, Percent_Identity=29.8245614035088, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=337, Percent_Identity=28.1899109792285, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17550882, Length=341, Percent_Identity=28.7390029325513, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319830, Length=344, Percent_Identity=49.4186046511628, Blast_Score=300, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6322097, Length=342, Percent_Identity=38.0116959064327, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324709, Length=344, Percent_Identity=33.4302325581395, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=359, Percent_Identity=32.3119777158774, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=31.6715542521994, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=31.6715542521994, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24661184, Length=353, Percent_Identity=30.028328611898, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI161078635, Length=357, Percent_Identity=29.9719887955182, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161078637, Length=356, Percent_Identity=29.4943820224719, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078633, Length=356, Percent_Identity=29.4943820224719, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24650122, Length=356, Percent_Identity=29.4943820224719, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078639, Length=354, Percent_Identity=29.3785310734463, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281362242, Length=345, Percent_Identity=26.6666666666667, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648872, Length=345, Percent_Identity=26.6666666666667, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI20130355, Length=344, Percent_Identity=24.1279069767442, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_DESDG (Q30WD0)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389711.1
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q30WD0
- SMR:   Q30WD0
- STRING:   Q30WD0
- GeneID:   3758197
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_3222
- NMPDR:   fig|207559.3.peg.2944
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   DDES207559:DDE_3222-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38011; Mature: 38011

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 96-96 BINDING 106-106 BINDING 135-135 BINDING 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMRICLLAGDGIGPEIMEQGVRVLETVAGKFGHDMEFTDALIGGAAIDETGDPLPQETV
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHH
EACKAAQAVLLGAVGGPRWDNLTGAERPEAGLLGIRKALGLFANLRPAKLFPELASACFL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
RPDIVANGIDVMVVRELTGGIYFGTPAGEETRDGLRTAFNTMVYNEEEVRRIARVAFEAA
CCHHHHCCCCEEEEEEHHCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RKRSGRVCSVDKANVLAVSRLWREIVTATHADEFPDVELSHMYVDNAAMQLVRDPSQFDV
HHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEHHHHHHHHHCCCCCEEE
IVTGNLFGDILSDEASVITGSIGMLPSASLGAGGPGLFEPIHGSAPDIAGQNKANPLATI
EEECHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LSTAMMLRHSFGLEKEAEAVEGAVQQVLKAGYRTGDIMDQGGTLVGCNTMGNLVNERI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHHCCC
>Mature Secondary Structure
MEMRICLLAGDGIGPEIMEQGVRVLETVAGKFGHDMEFTDALIGGAAIDETGDPLPQETV
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHH
EACKAAQAVLLGAVGGPRWDNLTGAERPEAGLLGIRKALGLFANLRPAKLFPELASACFL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
RPDIVANGIDVMVVRELTGGIYFGTPAGEETRDGLRTAFNTMVYNEEEVRRIARVAFEAA
CCHHHHCCCCEEEEEEHHCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RKRSGRVCSVDKANVLAVSRLWREIVTATHADEFPDVELSHMYVDNAAMQLVRDPSQFDV
HHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEHHHHHHHHHCCCCCEEE
IVTGNLFGDILSDEASVITGSIGMLPSASLGAGGPGLFEPIHGSAPDIAGQNKANPLATI
EEECHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LSTAMMLRHSFGLEKEAEAVEGAVQQVLKAGYRTGDIMDQGGTLVGCNTMGNLVNERI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA