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The map label for this gene is ntrC [H]

Identifier: 78358236

GI number: 78358236

Start: 3186048

End: 3187532

Strand: Direct

Name: ntrC [H]

Synonym: Dde_3196

Alternate gene names: 78358236

Gene position: 3186048-3187532 (Clockwise)

Preceding gene: 78358235

Following gene: 78358237

Centisome position: 85.41

GC content: 59.93

Gene sequence:

>1485_bases
GTGACCGCCCCGCTCACCATACCGTGCGAAACCATCATGCAGTCCATTGCGGACGGTGTTTTCACGGTTGACCTTCAGTG
GAACATCACTTTTTTCAATTCCGCAGCGGAATCCATAACCGGTATCCGCGCGCAGGAGGCCGTGGGCCGTAAATGCTGGG
AAGTTCTGCGCTCCAGCCTGTGCGACGGGCAATGCGCGCTTGATGCCTGCATGCACCGCAATGTCAACGTGTCGGGCAAG
TCCATTTTCATCGTCCGCAGCGACGGTACCCGTGTGCCCATATCCATCAGTGCCGCCCCGCTGCGTGACCCGCAGGGAGA
ACTTATCGGCGGGGTAGAAACATTCCGCGATCTTTCTGAAGTGACTCTGCTGCGCAAGGAAATGCAGAGCCAGTACGGCA
TTGAGGACATAGTGGGCAAAAGTCCGGCACTTTCGCGGATTTTTGCCATTCTGCCCCCCGTGGCACGCAGCGGCAGCAGC
GTGCTGCTGACCGGAGAATCCGGCACCGGCAAAGAACTGTTCGCCCGCGCACTGCACAACCACAGCGAACGGGCACAGCA
GCCCTTCATTGCGGTAAACTGCGCGGCACTGCCTCCGGCGTTGCTCGAATCTGAACTGTTCGGCTACAAAAAAGGCGCAT
TTACCGATGCGCGCAAGGACAAACCCGGCAGGCTGGCACTGGCACACGGCGGAACGCTTTTTCTGGATGAAATAGGAGAC
ATGCCTCTGCCGCTGCAGGCAAAACTGCTGCGCGTGCTGCAGGAGAAAAAATTTGATCCTCTGGGGGGCATTGCACCGGA
AGAAGCCGACGTTCGCATAATCGCCGCCACCAACAGCAATATGGAACAACTTGTTGAACAGGGCGGCTTCCGGCGTGACC
TGTATTTCAGGCTGAATGTAGTGCGCATGGAACTGCCCGCTCTGCGTGAACGGCGCAGCGATATCCCCCTTCTGGCCGCC
CATTTCGTGCAGCGGTTCAATACCCTGCAGAACAAACAGGTTCAGGGACTGTCCGAAGACGTTCTGGCCACCCTCATGCG
GCACGAATTTTCCGGCAACGTACGCGAGCTGGAAAACATTATCGAATACGCGTTCATTCTCTGCTCCTCCGGATTCATCC
AGATGGAGCACCTGCCTGATTACCTGCAGCACAGCACTGCGGCTTCGGGCGGTATCGGGCCTGACAGCGCGGCACACCCC
GCCCCGCGTACCCCCGGCACGACAAGCTCGCTGCCCCCCGCTGCATCCGCTCCCGACAGTGTAAACGGCGCGCACCGGCA
TGCGGCCTTCCCGCCATCCGTCCCGCAGGAGGCGCTTACACCGCAGAACGCCGCTGACCGGACCATGACCATGCGGGACA
TAAAAAAGCAGGCTGTGCTGCAGGCGCTGGCACGGCACGGAGGACGGCGCATGGCCGCCTGCCGCGACCTTGATATCAGC
AAAGACACGCTGCGCCGCATTCTGAACCAGCCTGACAAGGCGTAA

Upstream 100 bases:

>100_bases
GCGTGCAGGCTGCGCAGCCACTTTACCCCCGCGCACAGTTGGCGTACCATTGTCAGCAATCGCTGACTTCATACCGCCGC
ACCGGCCAAGGACCATACCC

Downstream 100 bases:

>100_bases
AACACGCCTGAAGCGCTCCGCATGGGGCGTATTATACGCCATCACAGGGCGCGTTTCACGCCCGAAAATCTGCTGCCGCC
TTCCACACAAACACACAAGC

Product: Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTAPLTIPCETIMQSIADGVFTVDLQWNITFFNSAAESITGIRAQEAVGRKCWEVLRSSLCDGQCALDACMHRNVNVSGK
SIFIVRSDGTRVPISISAAPLRDPQGELIGGVETFRDLSEVTLLRKEMQSQYGIEDIVGKSPALSRIFAILPPVARSGSS
VLLTGESGTGKELFARALHNHSERAQQPFIAVNCAALPPALLESELFGYKKGAFTDARKDKPGRLALAHGGTLFLDEIGD
MPLPLQAKLLRVLQEKKFDPLGGIAPEEADVRIIAATNSNMEQLVEQGGFRRDLYFRLNVVRMELPALRERRSDIPLLAA
HFVQRFNTLQNKQVQGLSEDVLATLMRHEFSGNVRELENIIEYAFILCSSGFIQMEHLPDYLQHSTAASGGIGPDSAAHP
APRTPGTTSSLPPAASAPDSVNGAHRHAAFPPSVPQEALTPQNAADRTMTMRDIKKQAVLQALARHGGRRMAACRDLDIS
KDTLRRILNQPDKA

Sequences:

>Translated_494_residues
MTAPLTIPCETIMQSIADGVFTVDLQWNITFFNSAAESITGIRAQEAVGRKCWEVLRSSLCDGQCALDACMHRNVNVSGK
SIFIVRSDGTRVPISISAAPLRDPQGELIGGVETFRDLSEVTLLRKEMQSQYGIEDIVGKSPALSRIFAILPPVARSGSS
VLLTGESGTGKELFARALHNHSERAQQPFIAVNCAALPPALLESELFGYKKGAFTDARKDKPGRLALAHGGTLFLDEIGD
MPLPLQAKLLRVLQEKKFDPLGGIAPEEADVRIIAATNSNMEQLVEQGGFRRDLYFRLNVVRMELPALRERRSDIPLLAA
HFVQRFNTLQNKQVQGLSEDVLATLMRHEFSGNVRELENIIEYAFILCSSGFIQMEHLPDYLQHSTAASGGIGPDSAAHP
APRTPGTTSSLPPAASAPDSVNGAHRHAAFPPSVPQEALTPQNAADRTMTMRDIKKQAVLQALARHGGRRMAACRDLDIS
KDTLRRILNQPDKA
>Mature_493_residues
TAPLTIPCETIMQSIADGVFTVDLQWNITFFNSAAESITGIRAQEAVGRKCWEVLRSSLCDGQCALDACMHRNVNVSGKS
IFIVRSDGTRVPISISAAPLRDPQGELIGGVETFRDLSEVTLLRKEMQSQYGIEDIVGKSPALSRIFAILPPVARSGSSV
LLTGESGTGKELFARALHNHSERAQQPFIAVNCAALPPALLESELFGYKKGAFTDARKDKPGRLALAHGGTLFLDEIGDM
PLPLQAKLLRVLQEKKFDPLGGIAPEEADVRIIAATNSNMEQLVEQGGFRRDLYFRLNVVRMELPALRERRSDIPLLAAH
FVQRFNTLQNKQVQGLSEDVLATLMRHEFSGNVRELENIIEYAFILCSSGFIQMEHLPDYLQHSTAASGGIGPDSAAHPA
PRTPGTTSSLPPAASAPDSVNGAHRHAAFPPSVPQEALTPQNAADRTMTMRDIKKQAVLQALARHGGRRMAACRDLDISK
DTLRRILNQPDKA

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=265, Percent_Identity=51.3207547169811, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI1788550, Length=274, Percent_Identity=46.3503649635037, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI87082117, Length=267, Percent_Identity=47.565543071161, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1790437, Length=355, Percent_Identity=39.4366197183099, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789233, Length=423, Percent_Identity=35.2245862884161, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788905, Length=239, Percent_Identity=51.8828451882845, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1789087, Length=253, Percent_Identity=48.2213438735178, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1786524, Length=297, Percent_Identity=42.4242424242424, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87082152, Length=236, Percent_Identity=46.1864406779661, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=42.7947598253275, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1787583, Length=262, Percent_Identity=39.6946564885496, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081858, Length=380, Percent_Identity=31.8421052631579, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1789828, Length=253, Percent_Identity=35.9683794466403, Blast_Score=141, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53854; Mature: 53723

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLTIPCETIMQSIADGVFTVDLQWNITFFNSAAESITGIRAQEAVGRKCWEVLRSSL
CCCCCCCCHHHHHHHHCCCEEEEEEEEEEEEECHHHHHHHCCHHHHHHHHHHHHHHHHHC
CDGQCALDACMHRNVNVSGKSIFIVRSDGTRVPISISAAPLRDPQGELIGGVETFRDLSE
CCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
VTLLRKEMQSQYGIEDIVGKSPALSRIFAILPPVARSGSSVLLTGESGTGKELFARALHN
HHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHCHHHCCCCEEEEECCCCCCHHHHHHHHHH
HSERAQQPFIAVNCAALPPALLESELFGYKKGAFTDARKDKPGRLALAHGGTLFLDEIGD
HHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHCC
MPLPLQAKLLRVLQEKKFDPLGGIAPEEADVRIIAATNSNMEQLVEQGGFRRDLYFRLNV
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCEEEEEEEEH
VRMELPALRERRSDIPLLAAHFVQRFNTLQNKQVQGLSEDVLATLMRHEFSGNVRELENI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHH
IEYAFILCSSGFIQMEHLPDYLQHSTAASGGIGPDSAAHPAPRTPGTTSSLPPAASAPDS
HHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VNGAHRHAAFPPSVPQEALTPQNAADRTMTMRDIKKQAVLQALARHGGRRMAACRDLDIS
CCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCC
KDTLRRILNQPDKA
HHHHHHHHCCCCCC
>Mature Secondary Structure 
TAPLTIPCETIMQSIADGVFTVDLQWNITFFNSAAESITGIRAQEAVGRKCWEVLRSSL
CCCCCCCHHHHHHHHCCCEEEEEEEEEEEEECHHHHHHHCCHHHHHHHHHHHHHHHHHC
CDGQCALDACMHRNVNVSGKSIFIVRSDGTRVPISISAAPLRDPQGELIGGVETFRDLSE
CCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
VTLLRKEMQSQYGIEDIVGKSPALSRIFAILPPVARSGSSVLLTGESGTGKELFARALHN
HHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHCHHHCCCCEEEEECCCCCCHHHHHHHHHH
HSERAQQPFIAVNCAALPPALLESELFGYKKGAFTDARKDKPGRLALAHGGTLFLDEIGD
HHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHCC
MPLPLQAKLLRVLQEKKFDPLGGIAPEEADVRIIAATNSNMEQLVEQGGFRRDLYFRLNV
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCEEEEEEEEH
VRMELPALRERRSDIPLLAAHFVQRFNTLQNKQVQGLSEDVLATLMRHEFSGNVRELENI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHH
IEYAFILCSSGFIQMEHLPDYLQHSTAASGGIGPDSAAHPAPRTPGTTSSLPPAASAPDS
HHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VNGAHRHAAFPPSVPQEALTPQNAADRTMTMRDIKKQAVLQALARHGGRRMAACRDLDIS
CCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCC
KDTLRRILNQPDKA
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8095910 [H]