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The map label for this gene is pnp

Identifier: 78358207

GI number: 78358207

Start: 3154355

End: 3156607

Strand: Direct

Name: pnp

Synonym: Dde_3167

Alternate gene names: 78358207

Gene position: 3154355-3156607 (Clockwise)

Preceding gene: 78358206

Following gene: 78358209

Centisome position: 84.56

GC content: 60.81

Gene sequence:

>2253_bases
ATGGAAAATCTCTTCGGCGCCAAGCGCCTCACCGCCACCGTGGGCGGTAAAGAAATCATCATGGAAACCGGGCGACTGGC
CAATCAGGCCGACGGCGCGGTTTGGATTCAGTGCGGCGGCACCGTTGTGCTGTGCACCGTGTGCACGCAGCCGCTGGAAC
GCGAAATCAGCTTCTTTCCGCTGGTTGTTGACTACACCGAAAAGATGTACGCGGCAGGCCGCATTCCCGGCAGCTTTTTC
CGCCGCGAAATAGGCCGCCCCAGCGAGCGCGAAACTCTTGTTTCCCGCCTCATCGACCGTCCCTTGCGTCCGCTGTTCCC
CAAGGGTTTCACCAACGAAGTCCAGATTCTGGCCAATGTCATTTCCGCCGATCAGGAAAATGACTCCGACGTGCTGGCGC
TGTGCGGTTCCTCCGCCGCCGTCATGCTGTCCAGTGTCCCCTTTGAAGCTCCCGTGGCCGGTGCCCGCGTCTGCCGCATC
GACGGCGAATTTGTGCTTAACCCCACCATCACCGAAGCGGCAAAGGCCGACCTCAACCTTGTTGTGGCCGCCAACCGCGA
CGCTCTGGTCATGGTTGAGGGCGAAGCCGACTTCGTGCCCGAAGAAGTGATTGCGCAGGCTCTGGAATGGGCGCACCGCG
AAGTGCAGCCCATCATCGACATGCAGGAAAAGCTGCGCGAACTGGCAGGCAAGGCCAAAATCGAGTTCGTGGCTCCTGAA
GAAGACACTGAACTGAAGGAAAAGGCCGAAGCCCTTGCCACCGCAGATCTGGCAGCCGCACTGCAGGTCAGGGAAAAAAT
GGCCCGCAAGGAAGCCAAAAAGGCCGTAAAGGAAAAAGTGAAGGCCGCCCTGCTGCAGGACCCCGCCTTTGCCGAAACTC
CCGAACGCCTTGCCGCTCTGGGCGATATTCTGAGCAGCCTTGAAAAACGCATCGTGCGCCAGCGCATACTCAAAGAAGGC
CTGCGCATCGACGGCCGCGACACCAAAACCGTGCGCCCCATCATCATCGAAGCAGGTGCCCTGCCCCGCGCACACGGTTC
GGCTATTTTCTCGCGCGGCGAGACCAAAAGCCTTGTGGTGACCACTCTTGGCAGCACCACCGACGAACAGCGCATGGACT
CCCTTACCGGTGACGTAACCAAGCGCTTCATGCTGCATTACAACTTCCCGCCCTATTCGGTGGGTGAAGTGAAGCCCGTG
CGCGTTTCCCGCCGCGAAATCGGTCACGGTGCGCTGGCAGAACGCGCTCTGCGCCCCGTACTGCCCTCGCCCGACAGCTT
CCCCTTCACACTGCGCGTGGTTTCCGAAACCATGGAATCCAACGGCTCGTCCTCCATGGCCGCAGTATGCGGCGGCAGCC
TTTCGCTGATGGACGCCGGTGTGCCCGTCAAGGCTCCGGTGGCCGGCATTGCCATGGGTCTCATCAAGGAAGATGACCAG
TTCATCGTTCTTACCGACATTCTGGGTGATGAAGACGCTCTGGGCGATATGGACTTCAAAATCGCCGGCACCGCAGAAGG
CGTCACCGCCGTGCAGATGGACATCAAAATCGACGGCTTGCCCGCCGAAGTGATGGGCCGCGCACTGGCTCAGGCCCGCG
AAGCACGTCTGCACATTCTGAATGAAATGGCCAAGGTGCTGCCCGAGCCGCGTCAGGCGCTTTCCAAATACGCTCCCCAG
CTGAGCGTGGTGGAAGTGAACCCCGAAATCATCCGCGTGATCATCGGCCCCGGCGGTAAAAACATCAAAGCCATCACCTC
GGCCACGGGTGCATCCATCGACATCGAGGACAGCGGCAGAATCTCCATTTTTGCCCCCACCAAAGAGTCCATGGATATGG
CCCGCGAAATGGTCATGTACTACGACCAGCGCCCCGAACTGGGTAAAAACTACACCGCAAAAGTCCGCAAGATCATGGAG
ATCGGCGCCATTGTGGAAATTCTGCCCAATTGTGAAGCGCTGATTCACATCTCGCAGCTGGACACCTCGCGCGTGGAAAA
AACCGAGGACGTTGTGCAGCTGGGACAGGACGTGGAAGTCAAGGTCATCGAGATCAATGACGGACGCGTACGCGCCTCGC
GCAAGGCAGTTATGCTGGAAGCGCAGGGCGTGGAATGGGATCCGGCCGACACCGCACGCCCCCCGCGCAAACCCCGCGAC
CGTGACGACCGCGGAGACCGCGGCGGACGCGGCGACCGTGGTGACCGCGGCGGACGCAACGGACGCGGCGGCGATCGTCG
CGACCGTCGCTAA

Upstream 100 bases:

>100_bases
AGCCGCCCAACGCAAGTGAAAGGGGGGTCCGGGAAGAACGCCCTGTCAGGGCATGGCCTTCTCCCCGGAATCCCCTTCAC
GAGAAAAGGATTCTTACGTT

Downstream 100 bases:

>100_bases
CAGCGGCAGCGCACTGCAATAACAGGGCGCGGAGTGTATGCTCCGCGCCTTTTTCGTGCAGCCCGCGCTGCAATCTGTCT
CAACATACCGCACCGGTCCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MENLFGAKRLTATVGGKEIIMETGRLANQADGAVWIQCGGTVVLCTVCTQPLEREISFFPLVVDYTEKMYAAGRIPGSFF
RREIGRPSERETLVSRLIDRPLRPLFPKGFTNEVQILANVISADQENDSDVLALCGSSAAVMLSSVPFEAPVAGARVCRI
DGEFVLNPTITEAAKADLNLVVAANRDALVMVEGEADFVPEEVIAQALEWAHREVQPIIDMQEKLRELAGKAKIEFVAPE
EDTELKEKAEALATADLAAALQVREKMARKEAKKAVKEKVKAALLQDPAFAETPERLAALGDILSSLEKRIVRQRILKEG
LRIDGRDTKTVRPIIIEAGALPRAHGSAIFSRGETKSLVVTTLGSTTDEQRMDSLTGDVTKRFMLHYNFPPYSVGEVKPV
RVSRREIGHGALAERALRPVLPSPDSFPFTLRVVSETMESNGSSSMAAVCGGSLSLMDAGVPVKAPVAGIAMGLIKEDDQ
FIVLTDILGDEDALGDMDFKIAGTAEGVTAVQMDIKIDGLPAEVMGRALAQAREARLHILNEMAKVLPEPRQALSKYAPQ
LSVVEVNPEIIRVIIGPGGKNIKAITSATGASIDIEDSGRISIFAPTKESMDMAREMVMYYDQRPELGKNYTAKVRKIME
IGAIVEILPNCEALIHISQLDTSRVEKTEDVVQLGQDVEVKVIEINDGRVRASRKAVMLEAQGVEWDPADTARPPRKPRD
RDDRGDRGGRGDRGDRGGRNGRGGDRRDRR

Sequences:

>Translated_750_residues
MENLFGAKRLTATVGGKEIIMETGRLANQADGAVWIQCGGTVVLCTVCTQPLEREISFFPLVVDYTEKMYAAGRIPGSFF
RREIGRPSERETLVSRLIDRPLRPLFPKGFTNEVQILANVISADQENDSDVLALCGSSAAVMLSSVPFEAPVAGARVCRI
DGEFVLNPTITEAAKADLNLVVAANRDALVMVEGEADFVPEEVIAQALEWAHREVQPIIDMQEKLRELAGKAKIEFVAPE
EDTELKEKAEALATADLAAALQVREKMARKEAKKAVKEKVKAALLQDPAFAETPERLAALGDILSSLEKRIVRQRILKEG
LRIDGRDTKTVRPIIIEAGALPRAHGSAIFSRGETKSLVVTTLGSTTDEQRMDSLTGDVTKRFMLHYNFPPYSVGEVKPV
RVSRREIGHGALAERALRPVLPSPDSFPFTLRVVSETMESNGSSSMAAVCGGSLSLMDAGVPVKAPVAGIAMGLIKEDDQ
FIVLTDILGDEDALGDMDFKIAGTAEGVTAVQMDIKIDGLPAEVMGRALAQAREARLHILNEMAKVLPEPRQALSKYAPQ
LSVVEVNPEIIRVIIGPGGKNIKAITSATGASIDIEDSGRISIFAPTKESMDMAREMVMYYDQRPELGKNYTAKVRKIME
IGAIVEILPNCEALIHISQLDTSRVEKTEDVVQLGQDVEVKVIEINDGRVRASRKAVMLEAQGVEWDPADTARPPRKPRD
RDDRGDRGGRGDRGDRGGRNGRGGDRRDRR
>Mature_750_residues
MENLFGAKRLTATVGGKEIIMETGRLANQADGAVWIQCGGTVVLCTVCTQPLEREISFFPLVVDYTEKMYAAGRIPGSFF
RREIGRPSERETLVSRLIDRPLRPLFPKGFTNEVQILANVISADQENDSDVLALCGSSAAVMLSSVPFEAPVAGARVCRI
DGEFVLNPTITEAAKADLNLVVAANRDALVMVEGEADFVPEEVIAQALEWAHREVQPIIDMQEKLRELAGKAKIEFVAPE
EDTELKEKAEALATADLAAALQVREKMARKEAKKAVKEKVKAALLQDPAFAETPERLAALGDILSSLEKRIVRQRILKEG
LRIDGRDTKTVRPIIIEAGALPRAHGSAIFSRGETKSLVVTTLGSTTDEQRMDSLTGDVTKRFMLHYNFPPYSVGEVKPV
RVSRREIGHGALAERALRPVLPSPDSFPFTLRVVSETMESNGSSSMAAVCGGSLSLMDAGVPVKAPVAGIAMGLIKEDDQ
FIVLTDILGDEDALGDMDFKIAGTAEGVTAVQMDIKIDGLPAEVMGRALAQAREARLHILNEMAKVLPEPRQALSKYAPQ
LSVVEVNPEIIRVIIGPGGKNIKAITSATGASIDIEDSGRISIFAPTKESMDMAREMVMYYDQRPELGKNYTAKVRKIME
IGAIVEILPNCEALIHISQLDTSRVEKTEDVVQLGQDVEVKVIEINDGRVRASRKAVMLEAQGVEWDPADTARPPRKPRD
RDDRGDRGGRGDRGDRGGRNGRGGDRRDRR

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=726, Percent_Identity=38.4297520661157, Blast_Score=463, Evalue=1e-130,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=47.4674384949349, Blast_Score=622, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI115534063, Length=618, Percent_Identity=36.5695792880259, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI281362905, Length=718, Percent_Identity=39.5543175487465, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651641, Length=718, Percent_Identity=39.5543175487465, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651643, Length=718, Percent_Identity=39.5543175487465, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI161079377, Length=663, Percent_Identity=39.8190045248869, Blast_Score=439, Evalue=1e-123,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_DESDG (Q30WI5)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389656.1
- ProteinModelPortal:   Q30WI5
- SMR:   Q30WI5
- STRING:   Q30WI5
- GeneID:   3758142
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_3167
- NMPDR:   fig|207559.3.peg.2663
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   DDES207559:DDE_3167-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 81753; Mature: 81753

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLFGAKRLTATVGGKEIIMETGRLANQADGAVWIQCGGTVVLCTVCTQPLEREISFFP
CCCCCCCHHHEEECCCHHHHHHHCCCCCCCCCEEEEEECCEEEEEEHHCCHHHCCCCCEE
LVVDYTEKMYAAGRIPGSFFRREIGRPSERETLVSRLIDRPLRPLFPKGFTNEVQILANV
EEEHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
ISADQENDSDVLALCGSSAAVMLSSVPFEAPVAGARVCRIDGEFVLNPTITEAAKADLNL
HHCCCCCCCCEEEECCCCCEEHEECCCCCCCCCCCEEEEECCCEEECCCHHHHHCCCCEE
VVAANRDALVMVEGEADFVPEEVIAQALEWAHREVQPIIDMQEKLRELAGKAKIEFVAPE
EEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
EDTELKEKAEALATADLAAALQVREKMARKEAKKAVKEKVKAALLQDPAFAETPERLAAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
GDILSSLEKRIVRQRILKEGLRIDGRDTKTVRPIIIEAGALPRAHGSAIFSRGETKSLVV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCEEEE
TTLGSTTDEQRMDSLTGDVTKRFMLHYNFPPYSVGEVKPVRVSRREIGHGALAERALRPV
EECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEHHHHHCCCHHHHHHHHCCC
LPSPDSFPFTLRVVSETMESNGSSSMAAVCGGSLSLMDAGVPVKAPVAGIAMGLIKEDDQ
CCCCCCCCEEHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCCCHHHHHHHHEECCCC
FIVLTDILGDEDALGDMDFKIAGTAEGVTAVQMDIKIDGLPAEVMGRALAQAREARLHIL
EEEEEECCCCCCCCCCCCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
NEMAKVLPEPRQALSKYAPQLSVVEVNPEIIRVIIGPGGKNIKAITSATGASIDIEDSGR
HHHHHHCCCHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCEEEEECCCCCEEEECCCCE
ISIFAPTKESMDMAREMVMYYDQRPELGKNYTAKVRKIMEIGAIVEILPNCEALIHISQL
EEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHC
DTSRVEKTEDVVQLGQDVEVKVIEINDGRVRASRKAVMLEAQGVEWDPADTARPPRKPRD
CHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCCC
RDDRGDRGGRGDRGDRGGRNGRGGDRRDRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MENLFGAKRLTATVGGKEIIMETGRLANQADGAVWIQCGGTVVLCTVCTQPLEREISFFP
CCCCCCCHHHEEECCCHHHHHHHCCCCCCCCCEEEEEECCEEEEEEHHCCHHHCCCCCEE
LVVDYTEKMYAAGRIPGSFFRREIGRPSERETLVSRLIDRPLRPLFPKGFTNEVQILANV
EEEHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
ISADQENDSDVLALCGSSAAVMLSSVPFEAPVAGARVCRIDGEFVLNPTITEAAKADLNL
HHCCCCCCCCEEEECCCCCEEHEECCCCCCCCCCCEEEEECCCEEECCCHHHHHCCCCEE
VVAANRDALVMVEGEADFVPEEVIAQALEWAHREVQPIIDMQEKLRELAGKAKIEFVAPE
EEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
EDTELKEKAEALATADLAAALQVREKMARKEAKKAVKEKVKAALLQDPAFAETPERLAAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
GDILSSLEKRIVRQRILKEGLRIDGRDTKTVRPIIIEAGALPRAHGSAIFSRGETKSLVV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCEEEE
TTLGSTTDEQRMDSLTGDVTKRFMLHYNFPPYSVGEVKPVRVSRREIGHGALAERALRPV
EECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEHHHHHCCCHHHHHHHHCCC
LPSPDSFPFTLRVVSETMESNGSSSMAAVCGGSLSLMDAGVPVKAPVAGIAMGLIKEDDQ
CCCCCCCCEEHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCCCHHHHHHHHEECCCC
FIVLTDILGDEDALGDMDFKIAGTAEGVTAVQMDIKIDGLPAEVMGRALAQAREARLHIL
EEEEEECCCCCCCCCCCCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
NEMAKVLPEPRQALSKYAPQLSVVEVNPEIIRVIIGPGGKNIKAITSATGASIDIEDSGR
HHHHHHCCCHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCEEEEECCCCCEEEECCCCE
ISIFAPTKESMDMAREMVMYYDQRPELGKNYTAKVRKIMEIGAIVEILPNCEALIHISQL
EEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHC
DTSRVEKTEDVVQLGQDVEVKVIEINDGRVRASRKAVMLEAQGVEWDPADTARPPRKPRD
CHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCCC
RDDRGDRGGRGDRGDRGGRNGRGGDRRDRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA