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The map label for this gene is fliY [H]

Identifier: 78358110

GI number: 78358110

Start: 3061411

End: 3062232

Strand: Reverse

Name: fliY [H]

Synonym: Dde_3070

Alternate gene names: 78358110

Gene position: 3062232-3061411 (Counterclockwise)

Preceding gene: 78358111

Following gene: 78358109

Centisome position: 82.09

GC content: 56.2

Gene sequence:

>822_bases
ATGAAACGGATGCTGACTGCGGCGGTTGCCGCCGTGCTGCTGCTTGGTCTGGCCGCCGGAGCCTGGGCCGATGTGCGGCA
GGAACTTACCCGGCAGAGCACCATCGCCCGTGCGGTGGACAGAGGCGTGCTGAAGGTGGGGTTTTCCACGTTTGTTCCGT
GGGCCATGAAAGACAAGCAGGGTGAGTTCATCGGGTTCGAGGTGGATGTGGCCAGACGTCTGGCAGCTGATCTCGGGCTG
GAAGTGCAGTTTGTGCCCACCAAATGGTCGGGCATCATACCCGCGCTGCTTACGGGCAAATTTGATGTGATTATCGGCGG
CATGAGTGTGAAGCCGGACAGAAATATTTCCGTCAACTTCACTATTCCCTATGATTATGCCGGTATGGCCGTCATGGCGC
ACAAAGGGAAAGCCGCGGGCTTTTCGCGGCTGGAAGATTTTGACCGGGAGGACGTGGTTGTGGTGGCCCGTATAGGCACC
ACGGCAGCTTCCGTGGCCAAAAAATATTTTCCTGCAGCGCAGGTGCGTCTGTTTGATGACGAAGCGCAGGCGGTACAGGA
ACTGCTTAACGGACGTGCCCACGCCATGGTTTCCAGCGCCCCGCTGCCTGCCTTTACCGTGCTGGAACATCCCGACACGC
TTTTTATGCCGGTGGAAGGAACCTTTACCCGTGAGCCTGTGGGATTTGCCGTGCGTAAAGGCGACATAGACACGCTGAAT
GTGTTCAACAACTGGATACGCATGGTCGAATCAGAAGGCTGGCTGAAAGAGCGCAAGCATTACTGGTTTGAAACAAAAGA
CTGGGAAAGCAGGATTAAATAA

Upstream 100 bases:

>100_bases
TCGTTACGCTCAGTCTTTCACTGCTGGCACAGCGCCTTGAGTGCCGGCTGAGAACCGGATCAAAGGCATGTCGCAAGGCT
GCCGCATAAGGAGGATTTGT

Downstream 100 bases:

>100_bases
CTCCGGAGCGCCGCGGCGGAAACTGTGCGGCTTCCGCCGCGGCAGCCGGTATTGTGCCGCGTTGCCGACCTTTTCATGAA
AAAAAACACACACCGGTTCT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKRMLTAAVAAVLLLGLAAGAWADVRQELTRQSTIARAVDRGVLKVGFSTFVPWAMKDKQGEFIGFEVDVARRLAADLGL
EVQFVPTKWSGIIPALLTGKFDVIIGGMSVKPDRNISVNFTIPYDYAGMAVMAHKGKAAGFSRLEDFDREDVVVVARIGT
TAASVAKKYFPAAQVRLFDDEAQAVQELLNGRAHAMVSSAPLPAFTVLEHPDTLFMPVEGTFTREPVGFAVRKGDIDTLN
VFNNWIRMVESEGWLKERKHYWFETKDWESRIK

Sequences:

>Translated_273_residues
MKRMLTAAVAAVLLLGLAAGAWADVRQELTRQSTIARAVDRGVLKVGFSTFVPWAMKDKQGEFIGFEVDVARRLAADLGL
EVQFVPTKWSGIIPALLTGKFDVIIGGMSVKPDRNISVNFTIPYDYAGMAVMAHKGKAAGFSRLEDFDREDVVVVARIGT
TAASVAKKYFPAAQVRLFDDEAQAVQELLNGRAHAMVSSAPLPAFTVLEHPDTLFMPVEGTFTREPVGFAVRKGDIDTLN
VFNNWIRMVESEGWLKERKHYWFETKDWESRIK
>Mature_273_residues
MKRMLTAAVAAVLLLGLAAGAWADVRQELTRQSTIARAVDRGVLKVGFSTFVPWAMKDKQGEFIGFEVDVARRLAADLGL
EVQFVPTKWSGIIPALLTGKFDVIIGGMSVKPDRNISVNFTIPYDYAGMAVMAHKGKAAGFSRLEDFDREDVVVVARIGT
TAASVAKKYFPAAQVRLFDDEAQAVQELLNGRAHAMVSSAPLPAFTVLEHPDTLFMPVEGTFTREPVGFAVRKGDIDTLN
VFNNWIRMVESEGWLKERKHYWFETKDWESRIK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=263, Percent_Identity=28.1368821292776, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787031, Length=232, Percent_Identity=26.7241379310345, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787085, Length=273, Percent_Identity=23.8095238095238, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787088, Length=219, Percent_Identity=21.4611872146119, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30310; Mature: 30310

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRMLTAAVAAVLLLGLAAGAWADVRQELTRQSTIARAVDRGVLKVGFSTFVPWAMKDKQ
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEECCC
GEFIGFEVDVARRLAADLGLEVQFVPTKWSGIIPALLTGKFDVIIGGMSVKPDRNISVNF
CCEEEEEHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCEEEEEECEEECCCCCEEEEE
TIPYDYAGMAVMAHKGKAAGFSRLEDFDREDVVVVARIGTTAASVAKKYFPAAQVRLFDD
EECCCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECH
EAQAVQELLNGRAHAMVSSAPLPAFTVLEHPDTLFMPVEGTFTREPVGFAVRKGDIDTLN
HHHHHHHHHCCCHHEEEECCCCCEEEEEECCCEEEEECCCCCCCCCCCEEEECCCCCHHH
VFNNWIRMVESEGWLKERKHYWFETKDWESRIK
HHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure
MKRMLTAAVAAVLLLGLAAGAWADVRQELTRQSTIARAVDRGVLKVGFSTFVPWAMKDKQ
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEECCC
GEFIGFEVDVARRLAADLGLEVQFVPTKWSGIIPALLTGKFDVIIGGMSVKPDRNISVNF
CCEEEEEHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCEEEEEECEEECCCCCEEEEE
TIPYDYAGMAVMAHKGKAAGFSRLEDFDREDVVVVARIGTTAASVAKKYFPAAQVRLFDD
EECCCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECH
EAQAVQELLNGRAHAMVSSAPLPAFTVLEHPDTLFMPVEGTFTREPVGFAVRKGDIDTLN
HHHHHHHHHCCCHHEEEECCCCCEEEEEECCCEEEEECCCCCCCCCCCEEEECCCCCHHH
VFNNWIRMVESEGWLKERKHYWFETKDWESRIK
HHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]