Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 78358088

GI number: 78358088

Start: 3038829

End: 3040025

Strand: Reverse

Name: pleD [H]

Synonym: Dde_3048

Alternate gene names: 78358088

Gene position: 3040025-3038829 (Counterclockwise)

Preceding gene: 78358089

Following gene: 78358087

Centisome position: 81.5

GC content: 60.82

Gene sequence:

>1197_bases
TTGTCCGTCACCGGAAATTCAGTACTTTTTGCCAGAGAATATGAGCTGATAGCCGAAGCGGAAGCTGCATTGGCCCGTAT
GGCACCGGAGGCTCCGGCGCATGATGCGCTGGCAGGTCTGCTTGCGGGGTTCTGCAAAACGCTGCGGCAATGCGGCCGGC
TTATGTCCATGGGGGACAGAATGCAGGCGCAGCTGAGTGAACTGAACCGCGATATCGCGGCCAAAGAAGCGCGCTACCGC
TCCATTTTTCAGTGCGCGGCCGAAGGGGTTTTCCGTACCGGTGCAGACGGCAGCTTTGCGGAAGTCAATCCTGCCATGGC
AAACATTTTCGGCTGGTCTTCGGTGGATGCGTTCATGCAGCACAACACCAGCCTTGCTTCTTTGTTTTCATCGCCGCAGG
CGCACTGCGAATATCTGGACAGGCTGTGCAGCGCCGGAGCTGTTTCACGTTACGAAGCTGTTCTGCGACGCAGAGACGGC
ACGCCCGTATGGGTCGAAATCAGCACGCAGGCGCTGGAAACAGCTCCGGAGACGGCCTGCGGCGCCACGCATGTGGGCGT
GGTGCTTGATGTGACCGCCCACAGGCGCATGCTTGAAGAGCTGTCACACATGGCCCGTACCGATCCGCTGACCGGTCTTA
ACAACCGCCGTTTTTTTATGGAGGCAGCCCGTGCCGAAGTGGAGCGCATGCAAAGCAGCGGCAGGCCGCTCTCTTTGCTG
ATGCTCGACATAGACCACTTTAAACGGGTCAACGACACGTGGGGGCACGATGCCGGTGACATGGTGCTCAAACAGCTTTC
CTGCTGCCTTGCAGAGACCATGCGCGGACAGGATGTCTGCGCGCGGCTGGGCGGAGAAGAGTTTGCCGTGCTGCTGCCGG
AAACAGGCCACGCCGAGGCCTGCCGTGCCGCGGAAACACTGCGTCAGGCTGTGGCCGGCATGGCGTTTGCCGGGCATGGT
TTTTCCGTCACGTCGTCGCTGGGCCTTACCACACTGGATTCCGCGGGGCATGCCGTTTCGGGGCAGCGTCCTGTGCCGGG
GGGCGTTGCGGTCTCTGCCTGCTGCGGGCAGGTGGTGGAAATCCTGCTGAAGCGGGCCGACATTGCCCTGTATGCGGCAA
AAAAGAACGGCCGCAACCGTACGGAAGTATATCCTGCCCGTTGCACTGCCATATGCGCCGGGGCGTTATGCATCTGA

Upstream 100 bases:

>100_bases
ATTATTGATACCCTCAAATACGCGTGTAAGCGGTCATGGTGCCTTGCGGCAGCCGTGGCGGTGCTTCTTTGCACGGCAAC
ACTCAGGGGGGAATCGAGTT

Downstream 100 bases:

>100_bases
AGACAGTATCTGGACAGATATGCTGACCGGCATGCTTACCGGCACGCCTGCAGGCGCGGCACACAAAGCCGCAAGGCTTG
CGGCATGGCCGCAACATACA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSVTGNSVLFAREYELIAEAEAALARMAPEAPAHDALAGLLAGFCKTLRQCGRLMSMGDRMQAQLSELNRDIAAKEARYR
SIFQCAAEGVFRTGADGSFAEVNPAMANIFGWSSVDAFMQHNTSLASLFSSPQAHCEYLDRLCSAGAVSRYEAVLRRRDG
TPVWVEISTQALETAPETACGATHVGVVLDVTAHRRMLEELSHMARTDPLTGLNNRRFFMEAARAEVERMQSSGRPLSLL
MLDIDHFKRVNDTWGHDAGDMVLKQLSCCLAETMRGQDVCARLGGEEFAVLLPETGHAEACRAAETLRQAVAGMAFAGHG
FSVTSSLGLTTLDSAGHAVSGQRPVPGGVAVSACCGQVVEILLKRADIALYAAKKNGRNRTEVYPARCTAICAGALCI

Sequences:

>Translated_398_residues
MSVTGNSVLFAREYELIAEAEAALARMAPEAPAHDALAGLLAGFCKTLRQCGRLMSMGDRMQAQLSELNRDIAAKEARYR
SIFQCAAEGVFRTGADGSFAEVNPAMANIFGWSSVDAFMQHNTSLASLFSSPQAHCEYLDRLCSAGAVSRYEAVLRRRDG
TPVWVEISTQALETAPETACGATHVGVVLDVTAHRRMLEELSHMARTDPLTGLNNRRFFMEAARAEVERMQSSGRPLSLL
MLDIDHFKRVNDTWGHDAGDMVLKQLSCCLAETMRGQDVCARLGGEEFAVLLPETGHAEACRAAETLRQAVAGMAFAGHG
FSVTSSLGLTTLDSAGHAVSGQRPVPGGVAVSACCGQVVEILLKRADIALYAAKKNGRNRTEVYPARCTAICAGALCI
>Mature_397_residues
SVTGNSVLFAREYELIAEAEAALARMAPEAPAHDALAGLLAGFCKTLRQCGRLMSMGDRMQAQLSELNRDIAAKEARYRS
IFQCAAEGVFRTGADGSFAEVNPAMANIFGWSSVDAFMQHNTSLASLFSSPQAHCEYLDRLCSAGAVSRYEAVLRRRDGT
PVWVEISTQALETAPETACGATHVGVVLDVTAHRRMLEELSHMARTDPLTGLNNRRFFMEAARAEVERMQSSGRPLSLLM
LDIDHFKRVNDTWGHDAGDMVLKQLSCCLAETMRGQDVCARLGGEEFAVLLPETGHAEACRAAETLRQAVAGMAFAGHGF
SVTSSLGLTTLDSAGHAVSGQRPVPGGVAVSACCGQVVEILLKRADIALYAAKKNGRNRTEVYPARCTAICAGALCI

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=198, Percent_Identity=35.8585858585859, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788381, Length=338, Percent_Identity=29.585798816568, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081881, Length=289, Percent_Identity=30.4498269896194, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786584, Length=190, Percent_Identity=37.3684210526316, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787262, Length=181, Percent_Identity=33.1491712707182, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082007, Length=183, Percent_Identity=34.9726775956284, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787816, Length=176, Percent_Identity=34.6590909090909, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788085, Length=183, Percent_Identity=29.5081967213115, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787802, Length=197, Percent_Identity=29.4416243654822, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787541, Length=193, Percent_Identity=30.0518134715026, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=125, Percent_Identity=28, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=183, Percent_Identity=28.4153005464481, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788956, Length=134, Percent_Identity=38.0597014925373, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42720; Mature: 42589

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTGNSVLFAREYELIAEAEAALARMAPEAPAHDALAGLLAGFCKTLRQCGRLMSMGDR
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
MQAQLSELNRDIAAKEARYRSIFQCAAEGVFRTGADGSFAEVNPAMANIFGWSSVDAFMQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHCCHHHHHHCCHHHHHHHHH
HNTSLASLFSSPQAHCEYLDRLCSAGAVSRYEAVLRRRDGTPVWVEISTQALETAPETAC
CCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHCCCCHHC
GATHVGVVLDVTAHRRMLEELSHMARTDPLTGLNNRRFFMEAARAEVERMQSSGRPLSLL
CCCHHHHEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
MLDIDHFKRVNDTWGHDAGDMVLKQLSCCLAETMRGQDVCARLGGEEFAVLLPETGHAEA
EEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCHHH
CRAAETLRQAVAGMAFAGHGFSVTSSLGLTTLDSAGHAVSGQRPVPGGVAVSACCGQVVE
HHHHHHHHHHHHHHHHCCCCCEECCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
ILLKRADIALYAAKKNGRNRTEVYPARCTAICAGALCI
HHHHHCCCEEEEECCCCCCCCEECHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVTGNSVLFAREYELIAEAEAALARMAPEAPAHDALAGLLAGFCKTLRQCGRLMSMGDR
CCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
MQAQLSELNRDIAAKEARYRSIFQCAAEGVFRTGADGSFAEVNPAMANIFGWSSVDAFMQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHCCHHHHHHCCHHHHHHHHH
HNTSLASLFSSPQAHCEYLDRLCSAGAVSRYEAVLRRRDGTPVWVEISTQALETAPETAC
CCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHCCCCHHC
GATHVGVVLDVTAHRRMLEELSHMARTDPLTGLNNRRFFMEAARAEVERMQSSGRPLSLL
CCCHHHHEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
MLDIDHFKRVNDTWGHDAGDMVLKQLSCCLAETMRGQDVCARLGGEEFAVLLPETGHAEA
EEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCHHH
CRAAETLRQAVAGMAFAGHGFSVTSSLGLTTLDSAGHAVSGQRPVPGGVAVSACCGQVVE
HHHHHHHHHHHHHHHHCCCCCEECCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
ILLKRADIALYAAKKNGRNRTEVYPARCTAICAGALCI
HHHHHCCCEEEEECCCCCCCCEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA