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The map label for this gene is pflD [H]

Identifier: 78358079

GI number: 78358079

Start: 3028046

End: 3030472

Strand: Reverse

Name: pflD [H]

Synonym: Dde_3039

Alternate gene names: 78358079

Gene position: 3030472-3028046 (Counterclockwise)

Preceding gene: 78358085

Following gene: 78358078

Centisome position: 81.24

GC content: 58.55

Gene sequence:

>2427_bases
ATGAATGCAGTGACACACTCGTATCATGACGACACCGGACGGCGGCAGGCTGCCGGGCTGCCTCTTTCAGCACGGCTGCG
ACGGTTGAAAGCCGCTTATCTTGAAGCGAAGCCGAGCATCACCATAGGCCGCGCCATGGCCTACACCGAAATTGAAAAGG
CGTATCCCGACCTGCCGCCGGTGCTGCGCCGCGCCATGGGATTCCGGCGTGCCTGCGAAACCGCCCCCGTGCTTATTCAG
GACGACGAACTGATTGTGGGGCACCCCTGCGGAGCGCCGCGCGCGGGTGCTTTTTCACCGGATACGGCGTGGAAGTGGCT
GCGGGACGAACTGGATACCATAGGCACACGCCCGCAGGACCCGTACATGATAAGCGAAGCCGACAAGAAAATCATGCGTG
AGGAGCTGTTTCCTTTCTGGGAGGGGCGCTCGCTTGATGAAGTCTGCGAGGACGCCTTCCGCCGGGAAGGTCTGTGGGAG
TTTTGCGCCGAGGCCGGAATCAGCGACCTCAGCTACCATCATACCAGCGGCGGCGGTGATACCAGCCCCGGATACGATAT
TATCCTTTTCACCAAAGGTATTAACGGACTGAAGGCCGAGGCCGAGGCGCATCTTGAAAGACTGGATGCGGAAAATGCCG
GAGACAGCAGCGGGGCTGATTTCTACCGGGCGGCGGTTATCATCTGTGAAGGCGTTTTGCTGTATGCGGGCCGGGTGGCG
GCGCATGCGCGTCAGCTGGCCGCTGCGGAACAAAATCCCGCCCGCAGGGAGGAACTGCTTGCCATTGCCGAGGTTAACAC
CAGAGTGCCGGCCAACCCGCCCGCAACCTTTCATGAAGCCCTGCAGGCAGTCTGGACAATCCAGTCGCTGTTTCTGCTGG
AAGAAAACCAGTGCAGCACTTCACTGGGACGGTTCGACCAGTATGTATACCCCTGCTACGAGGCCGGCATCCGTAACGGG
ACACTGACCAGAGAGCAGGCTTTTGAACTGACAGGGTGCTTCATCATAAAATGCTCTGAGATGATCTGGTATACTCCCGG
AGCCACCGCCAGATATTTTGCAGGATACATGCCTTTTATCAACATGTGTGTGGGCGGGCAGAAGCGTGAAGGCGGTGATG
CCACCAACGATCTGACACTGCTGCTCATGGATGCGGTGCGCAGCGTCGGGGTGTACCAGCCCTCGCTTGCCTGCCGCATT
CACAACCAGTCACCGCAGGAATACCTTGAAAAAATAGCGGATGTCGTGCGGGCGGGTACGGGTATGCCCGCATGCCATTT
TGACGATGCCCATATCAGGATGATGCTCCGCAAGGGGTTCGACTTTGACGATGCGCGCGATTATTGCCTGATGGGCTGTG
TGGAACCCCAGAAGTCGGGGCGCATACATCAGTGGACAGCGGGCGGTTTTACCCAGTGGCCGGTGGCAGTCGAACTGGTG
TTCAACAGAGGCGTGCTGCGTTCGTACGGCAGACGCGTTGCGCCGGATACCGGCGACCCTGCAGGGTTTACCAGCTATGC
CCAGTTTGAGGCTGCCGTGAAAACACAGCTTGACTACATAATGGAAATGACGGCGCGCGGAACGGTTATCAACCAGAAAC
TGGTGCGTGACCTGATGCCCACGCCGTACATGTCGCTGTTTGTGGACGGATGCATGCAGACGGGCAAAGATGTCACCGCC
GGCGGCGCGGTGCTGTATGAAGGACCGGGAACCATATTTGCCGGTCTGGGAACCTACGCGGACAGCATGGCCGCAGTGCG
CAGGCTTGTCTTTGACGAGGCCAGATACACCATGGCTGAAATGAAGCAGGCTCTGGCTGCTGACTGGGCCGGCTTTGAAC
AGATGCGCCGGGACTGCCGTAACGCGCCCAAATACGGCAACGATGATGAGTACGCCGACGGCATAGCCCGCGACATCATT
GATTACACGGAAAAGACGATCAACGGGTTCAAGACACTGTACGCCCGTCTGATTCACGGCACCTTGTCGCAGTCGTTCAA
TACGCCGCTGGGCGAAATGGTGGGAGCAACCCCTGACGGACGCGCAGCCGGTGCACCGCTTTCCGACGGCATGAGTCCTT
CGCAGGGGGCGGACCGCAAGGGGCCCACGGCCATCATAAAGTCCGTGGGCAGGCTGAATGTGGAGTCCATGAGTCTGGGC
ATGGCACATAATTTTAAACTGGTGCACGGATGTCTGGAAACACAGGAAGGACGCGCGGGACTTGTTTCTCTGCTGAAAAC
GGCCTCAGTGCTGGGCAACGGACAGATGCAGTTCAACTATGTTGATGACAGCATGCTGCGCGATGCTCAGCGCCATCCGG
AACAATACCGTGATCTTATGGTGCGTGTTGCCGGCTACAGCGCTTTTTTTGTGGAGCTGTGCAAAGAGGTGCAGGACGAG
ATAATCAGCCGGACGGCGCTTCACTGA

Upstream 100 bases:

>100_bases
GCCTGTCCGGTTTATGTCAGGGGTGTTTGCGTGACGGTGCAGGCGGCATGCCGGCCGCTGTTTTTTCTGCCTTGAACACA
CCAGCAAAAGGAAGACAGGA

Downstream 100 bases:

>100_bases
CGTGGCAGGCCGCATTGACCGGAAATCATTGATTTGTATGTATGTGAGGGCCGCCGCGCGACGGGCGGCCCTTTCCTTGT
TTTTGTGCAGGCGCAGAGTA

Product: formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 808; Mature: 808

Protein sequence:

>808_residues
MNAVTHSYHDDTGRRQAAGLPLSARLRRLKAAYLEAKPSITIGRAMAYTEIEKAYPDLPPVLRRAMGFRRACETAPVLIQ
DDELIVGHPCGAPRAGAFSPDTAWKWLRDELDTIGTRPQDPYMISEADKKIMREELFPFWEGRSLDEVCEDAFRREGLWE
FCAEAGISDLSYHHTSGGGDTSPGYDIILFTKGINGLKAEAEAHLERLDAENAGDSSGADFYRAAVIICEGVLLYAGRVA
AHARQLAAAEQNPARREELLAIAEVNTRVPANPPATFHEALQAVWTIQSLFLLEENQCSTSLGRFDQYVYPCYEAGIRNG
TLTREQAFELTGCFIIKCSEMIWYTPGATARYFAGYMPFINMCVGGQKREGGDATNDLTLLLMDAVRSVGVYQPSLACRI
HNQSPQEYLEKIADVVRAGTGMPACHFDDAHIRMMLRKGFDFDDARDYCLMGCVEPQKSGRIHQWTAGGFTQWPVAVELV
FNRGVLRSYGRRVAPDTGDPAGFTSYAQFEAAVKTQLDYIMEMTARGTVINQKLVRDLMPTPYMSLFVDGCMQTGKDVTA
GGAVLYEGPGTIFAGLGTYADSMAAVRRLVFDEARYTMAEMKQALAADWAGFEQMRRDCRNAPKYGNDDEYADGIARDII
DYTEKTINGFKTLYARLIHGTLSQSFNTPLGEMVGATPDGRAAGAPLSDGMSPSQGADRKGPTAIIKSVGRLNVESMSLG
MAHNFKLVHGCLETQEGRAGLVSLLKTASVLGNGQMQFNYVDDSMLRDAQRHPEQYRDLMVRVAGYSAFFVELCKEVQDE
IISRTALH

Sequences:

>Translated_808_residues
MNAVTHSYHDDTGRRQAAGLPLSARLRRLKAAYLEAKPSITIGRAMAYTEIEKAYPDLPPVLRRAMGFRRACETAPVLIQ
DDELIVGHPCGAPRAGAFSPDTAWKWLRDELDTIGTRPQDPYMISEADKKIMREELFPFWEGRSLDEVCEDAFRREGLWE
FCAEAGISDLSYHHTSGGGDTSPGYDIILFTKGINGLKAEAEAHLERLDAENAGDSSGADFYRAAVIICEGVLLYAGRVA
AHARQLAAAEQNPARREELLAIAEVNTRVPANPPATFHEALQAVWTIQSLFLLEENQCSTSLGRFDQYVYPCYEAGIRNG
TLTREQAFELTGCFIIKCSEMIWYTPGATARYFAGYMPFINMCVGGQKREGGDATNDLTLLLMDAVRSVGVYQPSLACRI
HNQSPQEYLEKIADVVRAGTGMPACHFDDAHIRMMLRKGFDFDDARDYCLMGCVEPQKSGRIHQWTAGGFTQWPVAVELV
FNRGVLRSYGRRVAPDTGDPAGFTSYAQFEAAVKTQLDYIMEMTARGTVINQKLVRDLMPTPYMSLFVDGCMQTGKDVTA
GGAVLYEGPGTIFAGLGTYADSMAAVRRLVFDEARYTMAEMKQALAADWAGFEQMRRDCRNAPKYGNDDEYADGIARDII
DYTEKTINGFKTLYARLIHGTLSQSFNTPLGEMVGATPDGRAAGAPLSDGMSPSQGADRKGPTAIIKSVGRLNVESMSLG
MAHNFKLVHGCLETQEGRAGLVSLLKTASVLGNGQMQFNYVDDSMLRDAQRHPEQYRDLMVRVAGYSAFFVELCKEVQDE
IISRTALH
>Mature_808_residues
MNAVTHSYHDDTGRRQAAGLPLSARLRRLKAAYLEAKPSITIGRAMAYTEIEKAYPDLPPVLRRAMGFRRACETAPVLIQ
DDELIVGHPCGAPRAGAFSPDTAWKWLRDELDTIGTRPQDPYMISEADKKIMREELFPFWEGRSLDEVCEDAFRREGLWE
FCAEAGISDLSYHHTSGGGDTSPGYDIILFTKGINGLKAEAEAHLERLDAENAGDSSGADFYRAAVIICEGVLLYAGRVA
AHARQLAAAEQNPARREELLAIAEVNTRVPANPPATFHEALQAVWTIQSLFLLEENQCSTSLGRFDQYVYPCYEAGIRNG
TLTREQAFELTGCFIIKCSEMIWYTPGATARYFAGYMPFINMCVGGQKREGGDATNDLTLLLMDAVRSVGVYQPSLACRI
HNQSPQEYLEKIADVVRAGTGMPACHFDDAHIRMMLRKGFDFDDARDYCLMGCVEPQKSGRIHQWTAGGFTQWPVAVELV
FNRGVLRSYGRRVAPDTGDPAGFTSYAQFEAAVKTQLDYIMEMTARGTVINQKLVRDLMPTPYMSLFVDGCMQTGKDVTA
GGAVLYEGPGTIFAGLGTYADSMAAVRRLVFDEARYTMAEMKQALAADWAGFEQMRRDCRNAPKYGNDDEYADGIARDII
DYTEKTINGFKTLYARLIHGTLSQSFNTPLGEMVGATPDGRAAGAPLSDGMSPSQGADRKGPTAIIKSVGRLNVESMSLG
MAHNFKLVHGCLETQEGRAGLVSLLKTASVLGNGQMQFNYVDDSMLRDAQRHPEQYRDLMVRVAGYSAFFVELCKEVQDE
IISRTALH

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=792, Percent_Identity=33.9646464646465, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1787044, Length=803, Percent_Identity=32.6276463262765, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI48994926, Length=568, Percent_Identity=26.056338028169, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1787131, Length=551, Percent_Identity=23.7749546279492, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 89207; Mature: 89207

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVTHSYHDDTGRRQAAGLPLSARLRRLKAAYLEAKPSITIGRAMAYTEIEKAYPDLPP
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCH
VLRRAMGFRRACETAPVLIQDDELIVGHPCGAPRAGAFSPDTAWKWLRDELDTIGTRPQD
HHHHHHHHHHHHCCCCEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
PYMISEADKKIMREELFPFWEGRSLDEVCEDAFRREGLWEFCAEAGISDLSYHHTSGGGD
CCEECHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCC
TSPGYDIILFTKGINGLKAEAEAHLERLDAENAGDSSGADFYRAAVIICEGVLLYAGRVA
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AHARQLAAAEQNPARREELLAIAEVNTRVPANPPATFHEALQAVWTIQSLFLLEENQCST
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
SLGRFDQYVYPCYEAGIRNGTLTREQAFELTGCFIIKCSEMIWYTPGATARYFAGYMPFI
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCEEEEEECCEEEECCCCHHHHHHHHHHHH
NMCVGGQKREGGDATNDLTLLLMDAVRSVGVYQPSLACRIHNQSPQEYLEKIADVVRAGT
HHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
GMPACHFDDAHIRMMLRKGFDFDDARDYCLMGCVEPQKSGRIHQWTAGGFTQWPVAVELV
CCCCCCCCHHHHHHHHHHCCCCCCCCCHHEEECCCCCCCCCEEEEECCCCCCCCHHHHHH
FNRGVLRSYGRRVAPDTGDPAGFTSYAQFEAAVKTQLDYIMEMTARGTVINQKLVRDLMP
HHCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
TPYMSLFVDGCMQTGKDVTAGGAVLYEGPGTIFAGLGTYADSMAAVRRLVFDEARYTMAE
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
MKQALAADWAGFEQMRRDCRNAPKYGNDDEYADGIARDIIDYTEKTINGFKTLYARLIHG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSQSFNTPLGEMVGATPDGRAAGAPLSDGMSPSQGADRKGPTAIIKSVGRLNVESMSLG
HHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHC
MAHNFKLVHGCLETQEGRAGLVSLLKTASVLGNGQMQFNYVDDSMLRDAQRHPEQYRDLM
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCHHHHHHHH
VRVAGYSAFFVELCKEVQDEIISRTALH
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNAVTHSYHDDTGRRQAAGLPLSARLRRLKAAYLEAKPSITIGRAMAYTEIEKAYPDLPP
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCH
VLRRAMGFRRACETAPVLIQDDELIVGHPCGAPRAGAFSPDTAWKWLRDELDTIGTRPQD
HHHHHHHHHHHHCCCCEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
PYMISEADKKIMREELFPFWEGRSLDEVCEDAFRREGLWEFCAEAGISDLSYHHTSGGGD
CCEECHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCC
TSPGYDIILFTKGINGLKAEAEAHLERLDAENAGDSSGADFYRAAVIICEGVLLYAGRVA
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AHARQLAAAEQNPARREELLAIAEVNTRVPANPPATFHEALQAVWTIQSLFLLEENQCST
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
SLGRFDQYVYPCYEAGIRNGTLTREQAFELTGCFIIKCSEMIWYTPGATARYFAGYMPFI
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCEEEEEECCEEEECCCCHHHHHHHHHHHH
NMCVGGQKREGGDATNDLTLLLMDAVRSVGVYQPSLACRIHNQSPQEYLEKIADVVRAGT
HHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
GMPACHFDDAHIRMMLRKGFDFDDARDYCLMGCVEPQKSGRIHQWTAGGFTQWPVAVELV
CCCCCCCCHHHHHHHHHHCCCCCCCCCHHEEECCCCCCCCCEEEEECCCCCCCCHHHHHH
FNRGVLRSYGRRVAPDTGDPAGFTSYAQFEAAVKTQLDYIMEMTARGTVINQKLVRDLMP
HHCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
TPYMSLFVDGCMQTGKDVTAGGAVLYEGPGTIFAGLGTYADSMAAVRRLVFDEARYTMAE
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
MKQALAADWAGFEQMRRDCRNAPKYGNDDEYADGIARDIIDYTEKTINGFKTLYARLIHG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSQSFNTPLGEMVGATPDGRAAGAPLSDGMSPSQGADRKGPTAIIKSVGRLNVESMSLG
HHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHC
MAHNFKLVHGCLETQEGRAGLVSLLKTASVLGNGQMQFNYVDDSMLRDAQRHPEQYRDLM
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCHHHHHHHH
VRVAGYSAFFVELCKEVQDEIISRTALH
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]