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The map label for this gene is pyrB

Identifier: 78358004

GI number: 78358004

Start: 2947154

End: 2948110

Strand: Direct

Name: pyrB

Synonym: Dde_2964

Alternate gene names: 78358004

Gene position: 2947154-2948110 (Clockwise)

Preceding gene: 78358003

Following gene: 78358005

Centisome position: 79.01

GC content: 60.5

Gene sequence:

>957_bases
ATGCAAGACCAAGAACGATTCATCTGGCCCCACAAAGATCTGCTGGACGTCACGCAACTCTCCCGTGAGCAAATCAGGCA
TCTGCTGGACCTTGCCGGACAGTTTCATGAAATAAACCAGCGCCCTGTAAAAAAGGTTCCCACCCTGAAAGGAAAAACCG
TGGTGCTGTTCTTTGCGGAGCCGAGCACCCGCACGAAAACATCGTTCGACATGGCGGGAAAACGCCTTTCAGCCGACACG
TTTTCACTGGCCAAATCAGGATCCAGTCTGGTCAAAGGCGAAAGCCTGAAAGATACCGCGCTGACCCTGCAGGCCATGTC
GCCGGATGTCATCGTCATCCGCCACACCTCCAGCGGGGCGGCACAGTTTCTGGCCGAACGGCTGGAATGCGCCGTGGTCA
ACGCGGGCGACGGCTGGCACGCACACCCCACACAGGCGCTGCTTGACGCATTTTCGCTCAACCGCGCATGGAACGGACAA
TTTGAAGGCAAGACTCTGCTTATTCTGGGCGATATTGCCCACAGCCGTGTTGCACGGTCCAACGTGCATCTGCTCACCCG
CATGGGTGCCAGAGTGCGCCTGTGCGCGCCGCGCACCCTGCTGCCGGCAGGCGCCGACACATGGCCCGTCGAAATCTTTG
ACAATCTGAACAAAGCCGTTCAGGGGGTCGATGCCGTCATGGCGCTGCGTCTGCAGCTGGAACGCCAGCAGGCCGGTCTG
CTGCCCGACCTGCGCGAGTACGCCTCGCGCTACTGCCTGAACCTGAACCATATGCAGCATGCAGCCCCCGGTGCCAGGGT
GCTGCACCCCGGCCCCATGAACCGCGGTGTGGAAATTTCGTCCGCCCTTGCCGACGCCGGCCAGAGTCTGGTGCTTGACC
AGGTGGCTTCCGGCGTGGCCACACGCATGGCCATTCTGTTTCTTCATGCAACCCGCAAAGACACGGGAGACCGCTGA

Upstream 100 bases:

>100_bases
CCGGGCATGGCCGCCGGATGGGCCGTAGTGCCCGACGACCTCTGCCCCGCCTGAGCAGGCACGAACCGGGACATGTATCC
CGCAATGAGACCGGAAGACA

Downstream 100 bases:

>100_bases
TGTCTGATCATATTTCCGGCACACTGTTTGTTTCGGGCGCGCTTCTTTCCGGGCGCACGGTTGATGTCACCGTATCGGGC
GGACGCATTGCCGCCGTCAG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MQDQERFIWPHKDLLDVTQLSREQIRHLLDLAGQFHEINQRPVKKVPTLKGKTVVLFFAEPSTRTKTSFDMAGKRLSADT
FSLAKSGSSLVKGESLKDTALTLQAMSPDVIVIRHTSSGAAQFLAERLECAVVNAGDGWHAHPTQALLDAFSLNRAWNGQ
FEGKTLLILGDIAHSRVARSNVHLLTRMGARVRLCAPRTLLPAGADTWPVEIFDNLNKAVQGVDAVMALRLQLERQQAGL
LPDLREYASRYCLNLNHMQHAAPGARVLHPGPMNRGVEISSALADAGQSLVLDQVASGVATRMAILFLHATRKDTGDR

Sequences:

>Translated_318_residues
MQDQERFIWPHKDLLDVTQLSREQIRHLLDLAGQFHEINQRPVKKVPTLKGKTVVLFFAEPSTRTKTSFDMAGKRLSADT
FSLAKSGSSLVKGESLKDTALTLQAMSPDVIVIRHTSSGAAQFLAERLECAVVNAGDGWHAHPTQALLDAFSLNRAWNGQ
FEGKTLLILGDIAHSRVARSNVHLLTRMGARVRLCAPRTLLPAGADTWPVEIFDNLNKAVQGVDAVMALRLQLERQQAGL
LPDLREYASRYCLNLNHMQHAAPGARVLHPGPMNRGVEISSALADAGQSLVLDQVASGVATRMAILFLHATRKDTGDR
>Mature_318_residues
MQDQERFIWPHKDLLDVTQLSREQIRHLLDLAGQFHEINQRPVKKVPTLKGKTVVLFFAEPSTRTKTSFDMAGKRLSADT
FSLAKSGSSLVKGESLKDTALTLQAMSPDVIVIRHTSSGAAQFLAERLECAVVNAGDGWHAHPTQALLDAFSLNRAWNGQ
FEGKTLLILGDIAHSRVARSNVHLLTRMGARVRLCAPRTLLPAGADTWPVEIFDNLNKAVQGVDAVMALRLQLERQQAGL
LPDLREYASRYCLNLNHMQHAAPGARVLHPGPMNRGVEISSALADAGQSLVLDQVASGVATRMAILFLHATRKDTGDR

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=302, Percent_Identity=32.7814569536424, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI2367364, Length=317, Percent_Identity=29.0220820189274, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=25.9938837920489, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786469, Length=326, Percent_Identity=26.3803680981595, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=324, Percent_Identity=30.5555555555556, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322331, Length=304, Percent_Identity=30.5921052631579, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24642586, Length=319, Percent_Identity=30.0940438871473, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_DESDG (Q30X38)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389453.1
- HSSP:   P96108
- ProteinModelPortal:   Q30X38
- SMR:   Q30X38
- STRING:   Q30X38
- GeneID:   3757969
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2964
- NMPDR:   fig|207559.3.peg.2986
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   DDES207559:DDE_2964-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34901; Mature: 34901

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDQERFIWPHKDLLDVTQLSREQIRHLLDLAGQFHEINQRPVKKVPTLKGKTVVLFFAE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEC
PSTRTKTSFDMAGKRLSADTFSLAKSGSSLVKGESLKDTALTLQAMSPDVIVIRHTSSGA
CCCCCCCHHHHCCCCCCCHHHHHHHCCCHHHCCCCCCHHHEEEEECCCCEEEEEECCCCH
AQFLAERLECAVVNAGDGWHAHPTQALLDAFSLNRAWNGQFEGKTLLILGDIAHSRVARS
HHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHH
NVHLLTRMGARVRLCAPRTLLPAGADTWPVEIFDNLNKAVQGVDAVMALRLQLERQQAGL
HHHHHHHCCCEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPDLREYASRYCLNLNHMQHAAPGARVLHPGPMNRGVEISSALADAGQSLVLDQVASGVA
CHHHHHHHHHHHCCCHHHHHCCCCCEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHH
TRMAILFLHATRKDTGDR
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQDQERFIWPHKDLLDVTQLSREQIRHLLDLAGQFHEINQRPVKKVPTLKGKTVVLFFAE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEC
PSTRTKTSFDMAGKRLSADTFSLAKSGSSLVKGESLKDTALTLQAMSPDVIVIRHTSSGA
CCCCCCCHHHHCCCCCCCHHHHHHHCCCHHHCCCCCCHHHEEEEECCCCEEEEEECCCCH
AQFLAERLECAVVNAGDGWHAHPTQALLDAFSLNRAWNGQFEGKTLLILGDIAHSRVARS
HHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHH
NVHLLTRMGARVRLCAPRTLLPAGADTWPVEIFDNLNKAVQGVDAVMALRLQLERQQAGL
HHHHHHHCCCEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPDLREYASRYCLNLNHMQHAAPGARVLHPGPMNRGVEISSALADAGQSLVLDQVASGVA
CHHHHHHHHHHHCCCHHHHHCCCCCEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHH
TRMAILFLHATRKDTGDR
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA