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The map label for this gene is lacA [C]

Identifier: 78357922

GI number: 78357922

Start: 2870045

End: 2870665

Strand: Reverse

Name: lacA [C]

Synonym: Dde_2881

Alternate gene names: 78357922

Gene position: 2870665-2870045 (Counterclockwise)

Preceding gene: 78357923

Following gene: 78357921

Centisome position: 76.96

GC content: 46.38

Gene sequence:

>621_bases
ATGCTTAGAATTAAGGAAATGATTGGTGCAATGCTGCGCCGTTCTTGTTGCGTTAAGCGTTTTACCGATCTGTTCGCAGG
CAGGTCACAGCAACGGTATAATTGTGAAGCACATTCCACTGTTCGCTTTTTTGATGAGGCGCGTATTATAAATAGCGCTG
GCGTTCCCTCAGCTATAACGATTGGGTCTGATACAATTATCAGAGGGGAACTGCTCACTTTTTTTGAAGCTGGGAGCATT
ACAATTGGCCGTTATTGCTTCATTGGAGAGGGTACCCGGATTTGGTCGGCAAAAAGAATTGTTGTCGGAGATCGAGTTTT
GATTTCGCATCACGTCAACATCTTTGATAACGACACTCACCCGATTGATGATGTCGAAGCGCGTCATCGCCAGTTCCTCG
GAATTCTCAGAGGAAGCCCGGAGCTTGATGTTTCATTAAACGAAAGAGCTGTGCATATAGAGGATGATGTGCTCATTGGG
TGCCAGGCCGTTATTCTTAAAGGGGTGACCATTGGTGCAGGCTCTGTAATAGGAGCAGGAGCTGTTGTAACAAAGGATGT
ACCTCCTCGCGTTGTAGTTGCCGGCAATCCCGCTCAGATAATTAGGCATCTTGACTTTTGA

Upstream 100 bases:

>100_bases
TGACTTCATCGAGAGATGCTGCACGTCTTTCCGAGTGCTTTGACATCCATGTAGAGAAGCAGTACTTCAGCGATTATCTG
GCTTTTTCAAGAAGTGTAAT

Downstream 100 bases:

>100_bases
GGTTTTGAAAGAATGCAATGTGGTGACAGGCAGATGACTTGGGAAGAGGCAGTAAGTTGGCTTAGAATCCAACCCGACAA
AACCGAACTGGTTTCTGCTT

Product: putative acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MLRIKEMIGAMLRRSCCVKRFTDLFAGRSQQRYNCEAHSTVRFFDEARIINSAGVPSAITIGSDTIIRGELLTFFEAGSI
TIGRYCFIGEGTRIWSAKRIVVGDRVLISHHVNIFDNDTHPIDDVEARHRQFLGILRGSPELDVSLNERAVHIEDDVLIG
CQAVILKGVTIGAGSVIGAGAVVTKDVPPRVVVAGNPAQIIRHLDF

Sequences:

>Translated_206_residues
MLRIKEMIGAMLRRSCCVKRFTDLFAGRSQQRYNCEAHSTVRFFDEARIINSAGVPSAITIGSDTIIRGELLTFFEAGSI
TIGRYCFIGEGTRIWSAKRIVVGDRVLISHHVNIFDNDTHPIDDVEARHRQFLGILRGSPELDVSLNERAVHIEDDVLIG
CQAVILKGVTIGAGSVIGAGAVVTKDVPPRVVVAGNPAQIIRHLDF
>Mature_206_residues
MLRIKEMIGAMLRRSCCVKRFTDLFAGRSQQRYNCEAHSTVRFFDEARIINSAGVPSAITIGSDTIIRGELLTFFEAGSI
TIGRYCFIGEGTRIWSAKRIVVGDRVLISHHVNIFDNDTHPIDDVEARHRQFLGILRGSPELDVSLNERAVHIEDDVLIG
CQAVILKGVTIGAGSVIGAGAVVTKDVPPRVVVAGNPAQIIRHLDF

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=205, Percent_Identity=31.7073170731707, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1786664, Length=105, Percent_Identity=43.8095238095238, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322243, Length=182, Percent_Identity=31.3186813186813, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 22622; Mature: 22622

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIKEMIGAMLRRSCCVKRFTDLFAGRSQQRYNCEAHSTVRFFDEARIINSAGVPSAIT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHEECCCCCCCEEE
IGSDTIIRGELLTFFEAGSITIGRYCFIGEGTRIWSAKRIVVGDRVLISHHVNIFDNDTH
ECCCCEEEHHEEEEEECCCEEEEEEEEEECCCEEEEEEEEEECCEEEEEEEEEEEECCCC
PIDDVEARHRQFLGILRGSPELDVSLNERAVHIEDDVLIGCQAVILKGVTIGAGSVIGAG
CCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCEEECHHHHHHCCEEECCCCEECCC
AVVTKDVPPRVVVAGNPAQIIRHLDF
EEEECCCCCEEEEECCHHHHHHHCCC
>Mature Secondary Structure
MLRIKEMIGAMLRRSCCVKRFTDLFAGRSQQRYNCEAHSTVRFFDEARIINSAGVPSAIT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHEECCCCCCCEEE
IGSDTIIRGELLTFFEAGSITIGRYCFIGEGTRIWSAKRIVVGDRVLISHHVNIFDNDTH
ECCCCEEEHHEEEEEECCCEEEEEEEEEECCCEEEEEEEEEECCEEEEEEEEEEEECCCC
PIDDVEARHRQFLGILRGSPELDVSLNERAVHIEDDVLIGCQAVILKGVTIGAGSVIGAG
CCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCEEECHHHHHHCCEEECCCCEECCC
AVVTKDVPPRVVVAGNPAQIIRHLDF
EEEECCCCCEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]