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The map label for this gene is tgt

Identifier: 78357885

GI number: 78357885

Start: 2831856

End: 2832989

Strand: Direct

Name: tgt

Synonym: Dde_2844

Alternate gene names: 78357885

Gene position: 2831856-2832989 (Clockwise)

Preceding gene: 78357883

Following gene: 78357887

Centisome position: 75.92

GC content: 58.82

Gene sequence:

>1134_bases
ATGGCAACAATTGGTGAATTTACGCTACACGCGACGGACGGCAAGGCGCGTACCGGTGTGCTGCAGACAGCGCACGGACC
GGTGCGCACGCCCATCTTTATGCCGGTTGGCACGGTGGGCAGTGTGAAAGGCGTGGCTCCGGACGATCTGGATGCCATCG
GCGCTGAAATAATTCTCGGCAACACATACCACCTGTATCTGCGCCCCGGTGACGAGCTGGTGGCGCGGCGCGGCGGGCTG
CACGAATTCAACGCATGGCGCAAGCCCATACTTACCGACAGCGGCGGCTTTCAGGTTTTCAGCCTCAGCGGTCTGCGGCG
TATTTCCGAAGAAGGCGTGGAGTTCCGCTCACATCTGGACGGCTCCAAGCATCTTTTCACGCCGGAAAAAGTCGTTTCCA
TCCAGCGCAATCTCAACTCCGACATCATGATGGTGCTTGACGAGTGCGTGCCCTACGGCGCGGACAAAGACTACACAGCC
CGCTCGCTGAAAATGACCACCCGCTGGGCGCAGCGCTGCCGCGATGCTTATCCCGCGGGCAGCGGCGGCAACCTGATGTT
CGGCATCACGCAGGGCGGCTTTTTTAAAGACCTGCGCGAGGAATCCATAGGCGAGCTGACCCGCATCGACTTTGACGGTT
TTGCACTGGGCGGGCTGTCGGTGGGAGAAAGCAAAACGGAAATGATGGATATTCTCTATCATACCGCCCCCATGCTGCCC
GCGCACAAACCGCGCTATCTCATGGGTGTGGGCACCCCGCTGGACATCATCAACGGCATCAACGCGGGCATAGACATGTT
CGACTGTGTGCTGCCCACCCGCAATGCCCGCAACGGCACGCTGTATACATCGGCCGGTAAAATAAACATCAAACGACGCG
AGTTTGCAGAAGACGACGGACCGCTTGACCCCAACTGTTCGTGCTACACCTGCCGCACGTTCAGCAGGGCGTACCTGCGC
CATCTCTTCCATGCGCAGGAAATCCTGTCATACAGACTCAACTCCATTCACAACCTGACGTACTTTCTCGACCTTGTACG
CGGTGCGCGTGAAGCCATCGCACAGGGAACCTTTGCGGAATACAAGAGCCGCTATGATGCCATCTACCCGCAGGAAGCCG
CGCTCTGCCCGTAA

Upstream 100 bases:

>100_bases
GCAATTATTTTTTACTTTGCCATGACATTTTTCCGGCACTCCGGCTTCGGGCGGCGTTTTTGACTTTTCAAGACTCCCTG
TCGCGGCTATGGTGCGCGCC

Downstream 100 bases:

>100_bases
ACCCGCCGCAGCGGACGCGTCGGGCTTCTGTGCCTGATGACTCTGCCAGCTCATACAGTGAAAGGCCGCACGGCATACCC
CGTGCGGCCTTTTTCATTCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MATIGEFTLHATDGKARTGVLQTAHGPVRTPIFMPVGTVGSVKGVAPDDLDAIGAEIILGNTYHLYLRPGDELVARRGGL
HEFNAWRKPILTDSGGFQVFSLSGLRRISEEGVEFRSHLDGSKHLFTPEKVVSIQRNLNSDIMMVLDECVPYGADKDYTA
RSLKMTTRWAQRCRDAYPAGSGGNLMFGITQGGFFKDLREESIGELTRIDFDGFALGGLSVGESKTEMMDILYHTAPMLP
AHKPRYLMGVGTPLDIINGINAGIDMFDCVLPTRNARNGTLYTSAGKINIKRREFAEDDGPLDPNCSCYTCRTFSRAYLR
HLFHAQEILSYRLNSIHNLTYFLDLVRGAREAIAQGTFAEYKSRYDAIYPQEAALCP

Sequences:

>Translated_377_residues
MATIGEFTLHATDGKARTGVLQTAHGPVRTPIFMPVGTVGSVKGVAPDDLDAIGAEIILGNTYHLYLRPGDELVARRGGL
HEFNAWRKPILTDSGGFQVFSLSGLRRISEEGVEFRSHLDGSKHLFTPEKVVSIQRNLNSDIMMVLDECVPYGADKDYTA
RSLKMTTRWAQRCRDAYPAGSGGNLMFGITQGGFFKDLREESIGELTRIDFDGFALGGLSVGESKTEMMDILYHTAPMLP
AHKPRYLMGVGTPLDIINGINAGIDMFDCVLPTRNARNGTLYTSAGKINIKRREFAEDDGPLDPNCSCYTCRTFSRAYLR
HLFHAQEILSYRLNSIHNLTYFLDLVRGAREAIAQGTFAEYKSRYDAIYPQEAALCP
>Mature_376_residues
ATIGEFTLHATDGKARTGVLQTAHGPVRTPIFMPVGTVGSVKGVAPDDLDAIGAEIILGNTYHLYLRPGDELVARRGGLH
EFNAWRKPILTDSGGFQVFSLSGLRRISEEGVEFRSHLDGSKHLFTPEKVVSIQRNLNSDIMMVLDECVPYGADKDYTAR
SLKMTTRWAQRCRDAYPAGSGGNLMFGITQGGFFKDLREESIGELTRIDFDGFALGGLSVGESKTEMMDILYHTAPMLPA
HKPRYLMGVGTPLDIINGINAGIDMFDCVLPTRNARNGTLYTSAGKINIKRREFAEDDGPLDPNCSCYTCRTFSRAYLRH
LFHAQEILSYRLNSIHNLTYFLDLVRGAREAIAQGTFAEYKSRYDAIYPQEAALCP

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=374, Percent_Identity=42.2459893048128, Blast_Score=313, Evalue=1e-85,
Organism=Escherichia coli, GI1786607, Length=360, Percent_Identity=53.0555555555556, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=40.2777777777778, Blast_Score=274, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI25146502, Length=136, Percent_Identity=28.6764705882353, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19920510, Length=354, Percent_Identity=44.0677966101695, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI21355311, Length=189, Percent_Identity=31.7460317460317, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_DESDG (Q30XF7)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389334.1
- HSSP:   P28720
- ProteinModelPortal:   Q30XF7
- SMR:   Q30XF7
- STRING:   Q30XF7
- GeneID:   3757867
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2844
- NMPDR:   fig|207559.3.peg.3651
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   DDES207559:DDE_2844-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41733; Mature: 41602

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIGEFTLHATDGKARTGVLQTAHGPVRTPIFMPVGTVGSVKGVAPDDLDAIGAEIILG
CCCCCCEEEEECCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCCHHHCCCEEEEC
NTYHLYLRPGDELVARRGGLHEFNAWRKPILTDSGGFQVFSLSGLRRISEEGVEFRSHLD
CEEEEEECCCHHHHHHCCCCHHHHHHCCCCEECCCCEEEEEHHHHHHHHHHHHHHHHHCC
GSKHLFTPEKVVSIQRNLNSDIMMVLDECVPYGADKDYTARSLKMTTRWAQRCRDAYPAG
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
SGGNLMFGITQGGFFKDLREESIGELTRIDFDGFALGGLSVGESKTEMMDILYHTAPMLP
CCCCEEEEECCCCHHHHHHHHHHHHHEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCC
AHKPRYLMGVGTPLDIINGINAGIDMFDCVLPTRNARNGTLYTSAGKINIKRREFAEDDG
CCCCCEEEECCCHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEECCCEEEEEHHHHCCCCC
PLDPNCSCYTCRTFSRAYLRHLFHAQEILSYRLNSIHNLTYFLDLVRGAREAIAQGTFAE
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
YKSRYDAIYPQEAALCP
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ATIGEFTLHATDGKARTGVLQTAHGPVRTPIFMPVGTVGSVKGVAPDDLDAIGAEIILG
CCCCCEEEEECCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCCHHHCCCEEEEC
NTYHLYLRPGDELVARRGGLHEFNAWRKPILTDSGGFQVFSLSGLRRISEEGVEFRSHLD
CEEEEEECCCHHHHHHCCCCHHHHHHCCCCEECCCCEEEEEHHHHHHHHHHHHHHHHHCC
GSKHLFTPEKVVSIQRNLNSDIMMVLDECVPYGADKDYTARSLKMTTRWAQRCRDAYPAG
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
SGGNLMFGITQGGFFKDLREESIGELTRIDFDGFALGGLSVGESKTEMMDILYHTAPMLP
CCCCEEEEECCCCHHHHHHHHHHHHHEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCC
AHKPRYLMGVGTPLDIINGINAGIDMFDCVLPTRNARNGTLYTSAGKINIKRREFAEDDG
CCCCCEEEECCCHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEECCCEEEEEHHHHCCCCC
PLDPNCSCYTCRTFSRAYLRHLFHAQEILSYRLNSIHNLTYFLDLVRGAREAIAQGTFAE
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
YKSRYDAIYPQEAALCP
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA