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The map label for this gene is livK [C]

Identifier: 78357872

GI number: 78357872

Start: 2816973

End: 2818100

Strand: Direct

Name: livK [C]

Synonym: Dde_2831

Alternate gene names: 78357872

Gene position: 2816973-2818100 (Clockwise)

Preceding gene: 78357864

Following gene: 78357873

Centisome position: 75.52

GC content: 61.97

Gene sequence:

>1128_bases
ATGCCATATCCTCGCGGCCGAACATTGTTTGCGCTCACACTGGGGGCAGCATGCCTGCTGATGGCGGCAGCCCTGTATCT
GGCAACGCCTCCCGGTGACATTCTGGTGGGCTTTTCCGGCCCGCTGGAAGGAAAATTCTCCGACCTCGGCGTGGAAGGCC
GTAACGCCGCGCAGCTGGCCATCGAAGACATCAACGCCGCAGGCGGTATTGACGGGCGGCGGCTGCGCCTTGTCACCGCA
CATGACGGTTACACGCAAAAAGACGCGGTGGAGGCCGACAGCCATCTTGCCGGACAGCGGGTGGCCGCCGTCATAGGGCA
CATGACCAGCAGCCAGCAGCTGGCCGTGATGGCCGTGCACAGGTCTGTGGTCTATGTCTCGCCCACGGTCTCCACGTATC
AGCTTACCGGCATGGATGACAATTTTTTCCGTGTCATTGCAGACAACGACGCCACAGCCCGTGATCTGGGCCTGTTTGCC
GCCTCCCGCGGGGTGCAGACGGCCGTGGCCCTGCTGGACACGGTGAATGAAGATTTTTCACGGACGTTTATCAACGCTGC
GGCCAGAGGCTTCACCTCGGCGGGAGGCACGGTAGTCAACATCATTCCGTTTTCCTCGTCCGTTCCTTCTTCGCTGCTGG
ATGCCGCCGCCTCGGCCGCTGATTCCGGCGCGCAGGCCGTATTTGTGGCGGCTTCTGCGCGTGACACCGCGTTTGTGCTG
GCCCAGATGGCCCAGAACGGATTCTCGGGCCCGAAGTTCTCCTCCACATGGGCACTGACTCCCGCGCTGATAGCACAGGG
CGGTACCGCAGTGGAAGACACGTTCTTCTGCCATCCTTATCCCACACTGGCCGAACTGCCCGCCCACCGCAACTTCATCA
GCCGGTTCAGCGAGCGGTTCGGCTGGGAACCGAGCTTTCCGGGCCTGTTTGCCTATACCGCCGTGCGGTTGCTGCAGCGC
GGGCTGGAAAAAACACAGGGACGGACGGCGGGCCTGCGTGAAGCCCTGAAAAGTATCAGACAACTGGATGGTCTGTGCGG
CCCCATCACGCTGGATGCATACGGAGACGCACACATGCCGTTCTTCATTTCCACAGTGCGCAACGGCGCATTCGAGCCGG
TACGATGA

Upstream 100 bases:

>100_bases
TGTCCGGTTATAAGGGGTTGCAAAAGCGGTGCATCAAAGTGATACTATAAGTATACAGCCACAGCCATCCGGCCGGTGCC
GGAGCACAGGGGGAAAAAGT

Downstream 100 bases:

>100_bases
AAACCACCCAGCTGCCTTCGCTTTCGCGGACCATGTCTGTCAAGCTTATCCGCTGGATGTGCGTGCTGGGAATGCTCTGC
GCGCTTTTTCTGGCCGCATT

Product: substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MPYPRGRTLFALTLGAACLLMAAALYLATPPGDILVGFSGPLEGKFSDLGVEGRNAAQLAIEDINAAGGIDGRRLRLVTA
HDGYTQKDAVEADSHLAGQRVAAVIGHMTSSQQLAVMAVHRSVVYVSPTVSTYQLTGMDDNFFRVIADNDATARDLGLFA
ASRGVQTAVALLDTVNEDFSRTFINAAARGFTSAGGTVVNIIPFSSSVPSSLLDAAASAADSGAQAVFVAASARDTAFVL
AQMAQNGFSGPKFSSTWALTPALIAQGGTAVEDTFFCHPYPTLAELPAHRNFISRFSERFGWEPSFPGLFAYTAVRLLQR
GLEKTQGRTAGLREALKSIRQLDGLCGPITLDAYGDAHMPFFISTVRNGAFEPVR

Sequences:

>Translated_375_residues
MPYPRGRTLFALTLGAACLLMAAALYLATPPGDILVGFSGPLEGKFSDLGVEGRNAAQLAIEDINAAGGIDGRRLRLVTA
HDGYTQKDAVEADSHLAGQRVAAVIGHMTSSQQLAVMAVHRSVVYVSPTVSTYQLTGMDDNFFRVIADNDATARDLGLFA
ASRGVQTAVALLDTVNEDFSRTFINAAARGFTSAGGTVVNIIPFSSSVPSSLLDAAASAADSGAQAVFVAASARDTAFVL
AQMAQNGFSGPKFSSTWALTPALIAQGGTAVEDTFFCHPYPTLAELPAHRNFISRFSERFGWEPSFPGLFAYTAVRLLQR
GLEKTQGRTAGLREALKSIRQLDGLCGPITLDAYGDAHMPFFISTVRNGAFEPVR
>Mature_374_residues
PYPRGRTLFALTLGAACLLMAAALYLATPPGDILVGFSGPLEGKFSDLGVEGRNAAQLAIEDINAAGGIDGRRLRLVTAH
DGYTQKDAVEADSHLAGQRVAAVIGHMTSSQQLAVMAVHRSVVYVSPTVSTYQLTGMDDNFFRVIADNDATARDLGLFAA
SRGVQTAVALLDTVNEDFSRTFINAAARGFTSAGGTVVNIIPFSSSVPSSLLDAAASAADSGAQAVFVAASARDTAFVLA
QMAQNGFSGPKFSSTWALTPALIAQGGTAVEDTFFCHPYPTLAELPAHRNFISRFSERFGWEPSFPGLFAYTAVRLLQRG
LEKTQGRTAGLREALKSIRQLDGLCGPITLDAYGDAHMPFFISTVRNGAFEPVR

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39661; Mature: 39530

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYPRGRTLFALTLGAACLLMAAALYLATPPGDILVGFSGPLEGKFSDLGVEGRNAAQLA
CCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHCCCCCCCHHEEE
IEDINAAGGIDGRRLRLVTAHDGYTQKDAVEADSHLAGQRVAAVIGHMTSSQQLAVMAVH
EEHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
RSVVYVSPTVSTYQLTGMDDNFFRVIADNDATARDLGLFAASRGVQTAVALLDTVNEDFS
CEEEEEECCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RTFINAAARGFTSAGGTVVNIIPFSSSVPSSLLDAAASAADSGAQAVFVAASARDTAFVL
HHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
AQMAQNGFSGPKFSSTWALTPALIAQGGTAVEDTFFCHPYPTLAELPAHRNFISRFSERF
HHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEECCCCHHHHCCHHHHHHHHHHHHC
GWEPSFPGLFAYTAVRLLQRGLEKTQGRTAGLREALKSIRQLDGLCGPITLDAYGDAHMP
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
FFISTVRNGAFEPVR
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
PYPRGRTLFALTLGAACLLMAAALYLATPPGDILVGFSGPLEGKFSDLGVEGRNAAQLA
CCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHCCCCCCCHHEEE
IEDINAAGGIDGRRLRLVTAHDGYTQKDAVEADSHLAGQRVAAVIGHMTSSQQLAVMAVH
EEHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
RSVVYVSPTVSTYQLTGMDDNFFRVIADNDATARDLGLFAASRGVQTAVALLDTVNEDFS
CEEEEEECCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RTFINAAARGFTSAGGTVVNIIPFSSSVPSSLLDAAASAADSGAQAVFVAASARDTAFVL
HHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
AQMAQNGFSGPKFSSTWALTPALIAQGGTAVEDTFFCHPYPTLAELPAHRNFISRFSERF
HHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEECCCCHHHHCCHHHHHHHHHHHHC
GWEPSFPGLFAYTAVRLLQRGLEKTQGRTAGLREALKSIRQLDGLCGPITLDAYGDAHMP
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
FFISTVRNGAFEPVR
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]