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The map label for this gene is pyrF

Identifier: 78357760

GI number: 78357760

Start: 2720483

End: 2721193

Strand: Reverse

Name: pyrF

Synonym: Dde_2718

Alternate gene names: 78357760

Gene position: 2721193-2720483 (Counterclockwise)

Preceding gene: 78357761

Following gene: 78357759

Centisome position: 72.95

GC content: 61.88

Gene sequence:

>711_bases
ATGATGATGCCGGAACTTGTGGTTGCTCTTGATTATCCTGAAAGCGAACAGGCTGTTGCCATGGCTGCACGGCTGCAAGG
AAGCTGCCCCTGGGTCAAGGTGGGGCTTGAACTGTACACGGCCGCCGGGCCGGACATCCTGCGCCGTCTCAAAGACATGG
GGTTTTCTGTTTTTCTCGATCTGAAGTTTTTCGACATTCCCAATACCGTCCGCGGTGCGGTGCGTTCATCGGTCTGCGCC
GGTGCGGACATGGTCAACATTCATCTGCTGGGGGGCGCCCGTATGGCGCAGGCCGCCGTGCAGGGGCTGCAGGAAGGCTG
TGAATGCAAGGGGCTTGCTGCGCCGCCGGTGCTGCTGGGCGTGACAGTGCTTACCAGTATGGCCCAGCAGGATCTGCCTG
CCGGTCTTGACCTGCCGCTGGACAGCGTGGTGGATGTGCTGGCGCGCTCCGGCAGAGAGTGGGGCTTGAACGGTGTCGTG
TGTTCGGGGTATGAAGTTGAAAGCATCAAAAAAAGCTGTGGTACAGACTACATCTGTCTTACTCCGGGCATCCGGCCCGC
CGCGGCGGATGACGACCAGCGCAGAACCATGACCCCGCAGCAGGCTGCGCGGGCCGGTTCCGACTATCTGGTTGTGGGCA
GACCCGTTACCGGAGCCGCTGACCCGGCCGGGGCCGCGTACAGCATCATGGAATCTGTCCGGCAGGCGTAG

Upstream 100 bases:

>100_bases
TGGAGCAGGCGTATGACGAGTTGCGTGCCGCGTATCTGGCTGCCACCCTGTCGCCCACCTGCCGCCCGGAACTGGTGGAC
GAACTGCTGCAGGATTGGAG

Downstream 100 bases:

>100_bases
ACCGCCTGTCATTGTCAGAAGGAGTGTGAATATGCAGCAGGAACAAGGCGCAGCTGCCGGCGGCAGGCAGGAAAAAAAAC
GTATCAAAGGCGTTTTTTCC

Product: orotidine-5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MMMPELVVALDYPESEQAVAMAARLQGSCPWVKVGLELYTAAGPDILRRLKDMGFSVFLDLKFFDIPNTVRGAVRSSVCA
GADMVNIHLLGGARMAQAAVQGLQEGCECKGLAAPPVLLGVTVLTSMAQQDLPAGLDLPLDSVVDVLARSGREWGLNGVV
CSGYEVESIKKSCGTDYICLTPGIRPAAADDDQRRTMTPQQAARAGSDYLVVGRPVTGAADPAGAAYSIMESVRQA

Sequences:

>Translated_236_residues
MMMPELVVALDYPESEQAVAMAARLQGSCPWVKVGLELYTAAGPDILRRLKDMGFSVFLDLKFFDIPNTVRGAVRSSVCA
GADMVNIHLLGGARMAQAAVQGLQEGCECKGLAAPPVLLGVTVLTSMAQQDLPAGLDLPLDSVVDVLARSGREWGLNGVV
CSGYEVESIKKSCGTDYICLTPGIRPAAADDDQRRTMTPQQAARAGSDYLVVGRPVTGAADPAGAAYSIMESVRQA
>Mature_236_residues
MMMPELVVALDYPESEQAVAMAARLQGSCPWVKVGLELYTAAGPDILRRLKDMGFSVFLDLKFFDIPNTVRGAVRSSVCA
GADMVNIHLLGGARMAQAAVQGLQEGCECKGLAAPPVLLGVTVLTSMAQQDLPAGLDLPLDSVVDVLARSGREWGLNGVV
CSGYEVESIKKSCGTDYICLTPGIRPAAADDDQRRTMTPQQAARAGSDYLVVGRPVTGAADPAGAAYSIMESVRQA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=233, Percent_Identity=43.7768240343348, Blast_Score=164, Evalue=4e-42,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_DESDG (Q30XT2)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389209.1
- ProteinModelPortal:   Q30XT2
- SMR:   Q30XT2
- STRING:   Q30XT2
- GeneID:   3757741
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2718
- NMPDR:   fig|207559.3.peg.3071
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK704924
- BioCyc:   DDES207559:DDE_2718-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24861; Mature: 24861

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 62-62 BINDING 11-11 BINDING 33-33 BINDING 125-125 BINDING 185-185 BINDING 193-193 BINDING 213-213 BINDING 214-214

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMPELVVALDYPESEQAVAMAARLQGSCPWVKVGLELYTAAGPDILRRLKDMGFSVFLD
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECHHHHCCCCHHHHHHHHHCCCEEEEE
LKFFDIPNTVRGAVRSSVCAGADMVNIHLLGGARMAQAAVQGLQEGCECKGLAAPPVLLG
EEEECCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHH
VTVLTSMAQQDLPAGLDLPLDSVVDVLARSGREWGLNGVVCSGYEVESIKKSCGTDYICL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHCCCEEEE
TPGIRPAAADDDQRRTMTPQQAARAGSDYLVVGRPVTGAADPAGAAYSIMESVRQA
CCCCCCCCCCCCHHHCCCHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MMMPELVVALDYPESEQAVAMAARLQGSCPWVKVGLELYTAAGPDILRRLKDMGFSVFLD
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECHHHHCCCCHHHHHHHHHCCCEEEEE
LKFFDIPNTVRGAVRSSVCAGADMVNIHLLGGARMAQAAVQGLQEGCECKGLAAPPVLLG
EEEECCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHH
VTVLTSMAQQDLPAGLDLPLDSVVDVLARSGREWGLNGVVCSGYEVESIKKSCGTDYICL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHCCCEEEE
TPGIRPAAADDDQRRTMTPQQAARAGSDYLVVGRPVTGAADPAGAAYSIMESVRQA
CCCCCCCCCCCCHHHCCCHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA