Click here to switch to the map view.

The map label for this gene is truA

Identifier: 78357749

GI number: 78357749

Start: 2708847

End: 2709683

Strand: Direct

Name: truA

Synonym: Dde_2707

Alternate gene names: 78357749

Gene position: 2708847-2709683 (Clockwise)

Preceding gene: 78357748

Following gene: 78357753

Centisome position: 72.62

GC content: 63.2

Gene sequence:

>837_bases
ATGGCCCGGCTGAAGCTTATCATAGCCTATGCAGGCACGCGCTATGCGGGCTGGCAGATACAGTGTCTTCCGGCCGGACG
GCCCCATGCCGTGCAGCAGCCCACAGTGCAGCAGGCGGTGGAAGATGCCGCAAAAGCCATACTGGGCAGCGCTGTCCGCG
TGCACGGAGCGGGGCGCACCGATTCGGGCGTTCACGCCGAAGGTCAGGTCGCCCATCTGGACATTCCCGATGCCAAAGCC
GCCGTTGACTGGCGGCGGGCTCTCAACGCCAAGCTGCCCGAAGACATATCGGTTCTGCAGGCCGCCGTTGTGCCGGACGG
CTTTCATGCCCGCTTTGACGCCGTAAAAAAACGCTACACCTACCGTATATGGCTTACCCGCGCTTTTGTGCTGCCGCAGC
GCCGTCCCTTTGTGCATATGACCGGCCCGCTGGACATCGCCGCCATGGACGCGGCAGCCGCGTTCATGACAGGCACACAC
GATTTCGCTTCGTTCCAGAATGCCGGAACCGAGCTGGCCACCACGGTACGCACGGTCTACAGCATAACACGCACGCCCGG
ACTTGCTCCCGATGATTTTCCGCGCAGCCACGACACTACCCCCCGTGCCCCTCTGGAACTGGCATGGCATTTTGAGGCAG
ACGGGTTTCTGAAGCAGATGGTACGCAACATGATGGGCCTGCTGCTGTGGACCGGTACGGGCAGATGCGCACCGGAGGAT
GTACCGGCTATCATCGCCGCCCGCGACAGGCGGCTTTCAGCCCCCACAGCCCCCGCATGCGGGCTGACCATGGAGCGTGT
TTTCTACCCGGACGAATGCTGCCCCCCCGAAAGCTGA

Upstream 100 bases:

>100_bases
CGTGGCAGGCAGGCTGGGGAAATTCTGTCGTTTGTCGATGCACAGAAAGCGGCCCGCCTTTCCGAGGCGCTGACCAAAAT
GCAGCTGCCGTTCGAGTAGT

Downstream 100 bases:

>100_bases
CAAAAAAAAGCGGCCGAAGCCGCTTGCATTCTTTCCGGAAATATCCGTAACCGGCAGCGCATATCAGGTATATCTGGGTT
CTTCCTCACGGATGACCTGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MARLKLIIAYAGTRYAGWQIQCLPAGRPHAVQQPTVQQAVEDAAKAILGSAVRVHGAGRTDSGVHAEGQVAHLDIPDAKA
AVDWRRALNAKLPEDISVLQAAVVPDGFHARFDAVKKRYTYRIWLTRAFVLPQRRPFVHMTGPLDIAAMDAAAAFMTGTH
DFASFQNAGTELATTVRTVYSITRTPGLAPDDFPRSHDTTPRAPLELAWHFEADGFLKQMVRNMMGLLLWTGTGRCAPED
VPAIIAARDRRLSAPTAPACGLTMERVFYPDECCPPES

Sequences:

>Translated_278_residues
MARLKLIIAYAGTRYAGWQIQCLPAGRPHAVQQPTVQQAVEDAAKAILGSAVRVHGAGRTDSGVHAEGQVAHLDIPDAKA
AVDWRRALNAKLPEDISVLQAAVVPDGFHARFDAVKKRYTYRIWLTRAFVLPQRRPFVHMTGPLDIAAMDAAAAFMTGTH
DFASFQNAGTELATTVRTVYSITRTPGLAPDDFPRSHDTTPRAPLELAWHFEADGFLKQMVRNMMGLLLWTGTGRCAPED
VPAIIAARDRRLSAPTAPACGLTMERVFYPDECCPPES
>Mature_277_residues
ARLKLIIAYAGTRYAGWQIQCLPAGRPHAVQQPTVQQAVEDAAKAILGSAVRVHGAGRTDSGVHAEGQVAHLDIPDAKAA
VDWRRALNAKLPEDISVLQAAVVPDGFHARFDAVKKRYTYRIWLTRAFVLPQRRPFVHMTGPLDIAAMDAAAAFMTGTHD
FASFQNAGTELATTVRTVYSITRTPGLAPDDFPRSHDTTPRAPLELAWHFEADGFLKQMVRNMMGLLLWTGTGRCAPEDV
PAIIAARDRRLSAPTAPACGLTMERVFYPDECCPPES

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=292, Percent_Identity=33.9041095890411, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI199559785, Length=295, Percent_Identity=27.4576271186441, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788657, Length=275, Percent_Identity=33.0909090909091, Blast_Score=137, Evalue=7e-34,
Organism=Drosophila melanogaster, GI116007752, Length=315, Percent_Identity=27.9365079365079, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_DESDG (Q30XU3)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389198.1
- ProteinModelPortal:   Q30XU3
- SMR:   Q30XU3
- STRING:   Q30XU3
- GeneID:   3757730
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2707
- NMPDR:   fig|207559.3.peg.3129
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   NDHARYA
- ProtClustDB:   PRK00021
- BioCyc:   DDES207559:DDE_2707-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30368; Mature: 30237

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLKLIIAYAGTRYAGWQIQCLPAGRPHAVQQPTVQQAVEDAAKAILGSAVRVHGAGRT
CCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCC
DSGVHAEGQVAHLDIPDAKAAVDWRRALNAKLPEDISVLQAAVVPDGFHARFDAVKKRYT
CCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHEE
YRIWLTRAFVLPQRRPFVHMTGPLDIAAMDAAAAFMTGTHDFASFQNAGTELATTVRTVY
EEEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHH
SITRTPGLAPDDFPRSHDTTPRAPLELAWHFEADGFLKQMVRNMMGLLLWTGTGRCAPED
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEEECCCCCCCCCC
VPAIIAARDRRLSAPTAPACGLTMERVFYPDECCPPES
CCEEEEECCCCCCCCCCCCCCCCHHHEECCCCCCCCCC
>Mature Secondary Structure 
ARLKLIIAYAGTRYAGWQIQCLPAGRPHAVQQPTVQQAVEDAAKAILGSAVRVHGAGRT
CCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCC
DSGVHAEGQVAHLDIPDAKAAVDWRRALNAKLPEDISVLQAAVVPDGFHARFDAVKKRYT
CCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHEE
YRIWLTRAFVLPQRRPFVHMTGPLDIAAMDAAAAFMTGTHDFASFQNAGTELATTVRTVY
EEEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHH
SITRTPGLAPDDFPRSHDTTPRAPLELAWHFEADGFLKQMVRNMMGLLLWTGTGRCAPED
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEEECCCCCCCCCC
VPAIIAARDRRLSAPTAPACGLTMERVFYPDECCPPES
CCEEEEECCCCCCCCCCCCCCCCHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA