Click here to switch to the map view.

The map label for this gene is cobS [H]

Identifier: 78357746

GI number: 78357746

Start: 2706826

End: 2707614

Strand: Direct

Name: cobS [H]

Synonym: Dde_2704

Alternate gene names: 78357746

Gene position: 2706826-2707614 (Clockwise)

Preceding gene: 78357745

Following gene: 78357747

Centisome position: 72.56

GC content: 62.1

Gene sequence:

>789_bases
ATGCCTGAACAGCAGCCTCCTGCCACCGCGTCCGGTTTACTGATCCGTGAGTGGCAGGTATTTCTGGACGGTCTGGCCTT
TATGACGCGGCTGAACCCCGCCCGTATGATTGCCGAAGGAGCTCTGGCCGCCACCATGCGTCAGATGCCGCTGTACGGGC
TGCTCATCGGGGCATTGTGCACGGTGCCGCTGTGGACGGGAATGACTGCGGGGCATGCGCTGGCCGGCAGCTGGCTGTAT
GTGGGGCTGGGCATGTGGCTTACACGCGGACTGCACTGGGACGGCTGGGCCGACCTGTGGGATGCATGGGGCAGCAGCGC
AACCGGTGACAGGTTCTGGCAGATAATGAAAGACAGCCGTACCGGTGCATTCGGCGTCATGGCCATAGTGCTCGGCATGG
GCGGCCAGATGATGTGTTCTGCCGAGATACTGCAGGGCATGCCTGCGGCACAGGCGGCAGGAGTGCTCATATGGGCGCCT
GCACTGGGACGCACGGCATGCGTGCTGTTGTCCTTTTCCGGCACACAGGCAGCGTGTTCGTCACTCGGCAGGCAGTTTCT
TGATGGCGCCACCCCTGCAGCGCTCGGCATCAGCGTAGTGCTGTGCGCCTTTTCCGGCGTCTGGCTGACAGGTGTCAGAA
TTCTGTTGCTTTCGCTGGTGTTTCTAGCTCCCGGCATTATTGCCCTCAGGCGGCTTTCGCGCAGGCAAAACGGCCTCAAC
GGCGATTTTATGGGCGCAATAATCATATGGGGCGAACTCTCGGCCCTGCTGGCGGGTGCTCTGGCCTGA

Upstream 100 bases:

>100_bases
CAGGGCTATCTTGAAATCGCCTGCAACTGCCGGCCTGCATCGCAGGTGCTCGGTCTGCGTACCGGCAGCAACATCATACT
CACCCCTGCGGAGAACTCCC

Downstream 100 bases:

>100_bases
TCTTTGCTTTGCCTGTAGCTGGCGACTGCACACGTTGCAGCCGTACATTCCGGCTATATGATATTGTCTTCACAGCACCC
ATTATCTGAAGAGGTTTTCC

Product: cobalamin-5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPEQQPPATASGLLIREWQVFLDGLAFMTRLNPARMIAEGALAATMRQMPLYGLLIGALCTVPLWTGMTAGHALAGSWLY
VGLGMWLTRGLHWDGWADLWDAWGSSATGDRFWQIMKDSRTGAFGVMAIVLGMGGQMMCSAEILQGMPAAQAAGVLIWAP
ALGRTACVLLSFSGTQAACSSLGRQFLDGATPAALGISVVLCAFSGVWLTGVRILLLSLVFLAPGIIALRRLSRRQNGLN
GDFMGAIIIWGELSALLAGALA

Sequences:

>Translated_262_residues
MPEQQPPATASGLLIREWQVFLDGLAFMTRLNPARMIAEGALAATMRQMPLYGLLIGALCTVPLWTGMTAGHALAGSWLY
VGLGMWLTRGLHWDGWADLWDAWGSSATGDRFWQIMKDSRTGAFGVMAIVLGMGGQMMCSAEILQGMPAAQAAGVLIWAP
ALGRTACVLLSFSGTQAACSSLGRQFLDGATPAALGISVVLCAFSGVWLTGVRILLLSLVFLAPGIIALRRLSRRQNGLN
GDFMGAIIIWGELSALLAGALA
>Mature_261_residues
PEQQPPATASGLLIREWQVFLDGLAFMTRLNPARMIAEGALAATMRQMPLYGLLIGALCTVPLWTGMTAGHALAGSWLYV
GLGMWLTRGLHWDGWADLWDAWGSSATGDRFWQIMKDSRTGAFGVMAIVLGMGGQMMCSAEILQGMPAAQAAGVLIWAPA
LGRTACVLLSFSGTQAACSSLGRQFLDGATPAALGISVVLCAFSGVWLTGVRILLLSLVFLAPGIIALRRLSRRQNGLNG
DFMGAIIIWGELSALLAGALA

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=242, Percent_Identity=29.3388429752066, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27696; Mature: 27565

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEQQPPATASGLLIREWQVFLDGLAFMTRLNPARMIAEGALAATMRQMPLYGLLIGALC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHH
TVPLWTGMTAGHALAGSWLYVGLGMWLTRGLHWDGWADLWDAWGSSATGDRFWQIMKDSR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCC
TGAFGVMAIVLGMGGQMMCSAEILQGMPAAQAAGVLIWAPALGRTACVLLSFSGTQAACS
CCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCCEEEECCCCCCEEEEEEECCCHHHHHH
SLGRQFLDGATPAALGISVVLCAFSGVWLTGVRILLLSLVFLAPGIIALRRLSRRQNGLN
HHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
GDFMGAIIIWGELSALLAGALA
CCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PEQQPPATASGLLIREWQVFLDGLAFMTRLNPARMIAEGALAATMRQMPLYGLLIGALC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHH
TVPLWTGMTAGHALAGSWLYVGLGMWLTRGLHWDGWADLWDAWGSSATGDRFWQIMKDSR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCC
TGAFGVMAIVLGMGGQMMCSAEILQGMPAAQAAGVLIWAPALGRTACVLLSFSGTQAACS
CCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCCEEEECCCCCCEEEEEEECCCHHHHHH
SLGRQFLDGATPAALGISVVLCAFSGVWLTGVRILLLSLVFLAPGIIALRRLSRRQNGLN
HHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
GDFMGAIIIWGELSALLAGALA
CCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA