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The map label for this gene is ycdT [H]

Identifier: 78357722

GI number: 78357722

Start: 2690094

End: 2690852

Strand: Reverse

Name: ycdT [H]

Synonym: Dde_2680

Alternate gene names: 78357722

Gene position: 2690852-2690094 (Counterclockwise)

Preceding gene: 78357723

Following gene: 78357721

Centisome position: 72.14

GC content: 60.34

Gene sequence:

>759_bases
ATGCTGACGACCAGAGCCGTTTCGCTGTTGCAGGCCCTGCGCAGGCCCCTGCAGGGTGCCGCACTGGGGCTGGGTGCCCC
TCTTGGCTGGTTTGTCATCAGGTGGTTGTGCGATATCCATCCTCAGCATCCGCTTTATGAACGCTGGCTGTTTGCCTACA
TGCTTGTGGGTACCGTGCTGGTATTCATGCTGTTCGGCCTTATCATCGGCAGGCACGAACGCCGGTTTGCCGGTCTCTGT
CTTATCGATCCTCTCACTTCTCTTTTCAACAAGCGTCATTTTGATCTGCGGCTGGGGCAGGAAGTGGCCCGCCATCAGCG
CGAGGACTCACCCATGTGTGTCATCATGCTCGATCTTGACCACTTCAAAAAAGTCAACGACACGCTGGGCCATCAGGCCG
GTGACGTGGTGCTGATGGCTGTGGCTTCCACACTGCGTGGCTGTGTGCGCACCGAAGACGTGCTGGCGCGCGTGGGTGGC
GAAGAATTTGCCGTGGTGGTGCCGCGTACGGACATGCACGGTGGCGCCGCGCTGGCGGAACGCATACGCGCGGCTGTGGA
AGAGCAGATAATTCCTGTGGGCGGCGGACGCGAGACCACCATAAGGGTGTCGCTGGGCGTGGCCTGTATGGAAGCCGGAT
CGGTGCCTGTATTGCCTCATGACCTTATGGAACAGGCGGATGAGGCTTTGTACAGGGCCAAGGCAAACGGCAGAAACCGA
GTGGAACTGGCCGCCCCCGCGGCGGAGAAAGGAGCGTGA

Upstream 100 bases:

>100_bases
GACAGGCTGGATTATGAAGACGTGTTCCGTATAGCACGGTCATGGATGCGCTGAACCGTGGCCGTCGGCAGGACGGCCCG
CCCCGTTAGGAGACCGCACC

Downstream 100 bases:

>100_bases
AGCAGCCATGAAGCAGCGTGGCGACGCGGTGCTGGTGCTGGGATGCGGCAGCATGGTGCGTGAAGTGGGCAGCCGTCTGG
GCGCATGTGGTGCTGATGTG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLTTRAVSLLQALRRPLQGAALGLGAPLGWFVIRWLCDIHPQHPLYERWLFAYMLVGTVLVFMLFGLIIGRHERRFAGLC
LIDPLTSLFNKRHFDLRLGQEVARHQREDSPMCVIMLDLDHFKKVNDTLGHQAGDVVLMAVASTLRGCVRTEDVLARVGG
EEFAVVVPRTDMHGGAALAERIRAAVEEQIIPVGGGRETTIRVSLGVACMEAGSVPVLPHDLMEQADEALYRAKANGRNR
VELAAPAAEKGA

Sequences:

>Translated_252_residues
MLTTRAVSLLQALRRPLQGAALGLGAPLGWFVIRWLCDIHPQHPLYERWLFAYMLVGTVLVFMLFGLIIGRHERRFAGLC
LIDPLTSLFNKRHFDLRLGQEVARHQREDSPMCVIMLDLDHFKKVNDTLGHQAGDVVLMAVASTLRGCVRTEDVLARVGG
EEFAVVVPRTDMHGGAALAERIRAAVEEQIIPVGGGRETTIRVSLGVACMEAGSVPVLPHDLMEQADEALYRAKANGRNR
VELAAPAAEKGA
>Mature_252_residues
MLTTRAVSLLQALRRPLQGAALGLGAPLGWFVIRWLCDIHPQHPLYERWLFAYMLVGTVLVFMLFGLIIGRHERRFAGLC
LIDPLTSLFNKRHFDLRLGQEVARHQREDSPMCVIMLDLDHFKKVNDTLGHQAGDVVLMAVASTLRGCVRTEDVLARVGG
EEFAVVVPRTDMHGGAALAERIRAAVEEQIIPVGGGRETTIRVSLGVACMEAGSVPVLPHDLMEQADEALYRAKANGRNR
VELAAPAAEKGA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=41.6149068322981, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786584, Length=196, Percent_Identity=32.6530612244898, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693134, Length=175, Percent_Identity=37.1428571428571, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=41.358024691358, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=39.622641509434, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787802, Length=139, Percent_Identity=38.8489208633094, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788381, Length=168, Percent_Identity=35.7142857142857, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87081974, Length=190, Percent_Identity=28.9473684210526, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=28.6585365853659, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=32.0224719101124, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=35.5828220858896, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=28.8343558282209, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI87081977, Length=172, Percent_Identity=29.6511627906977, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 27704; Mature: 27704

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTRAVSLLQALRRPLQGAALGLGAPLGWFVIRWLCDIHPQHPLYERWLFAYMLVGTVL
CCCHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VFMLFGLIIGRHERRFAGLCLIDPLTSLFNKRHFDLRLGQEVARHQREDSPMCVIMLDLD
HHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECHH
HFKKVNDTLGHQAGDVVLMAVASTLRGCVRTEDVLARVGGEEFAVVVPRTDMHGGAALAE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
RIRAAVEEQIIPVGGGRETTIRVSLGVACMEAGSVPVLPHDLMEQADEALYRAKANGRNR
HHHHHHHHCEECCCCCCCEEEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCE
VELAAPAAEKGA
EEEECCCCCCCC
>Mature Secondary Structure
MLTTRAVSLLQALRRPLQGAALGLGAPLGWFVIRWLCDIHPQHPLYERWLFAYMLVGTVL
CCCHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VFMLFGLIIGRHERRFAGLCLIDPLTSLFNKRHFDLRLGQEVARHQREDSPMCVIMLDLD
HHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECHH
HFKKVNDTLGHQAGDVVLMAVASTLRGCVRTEDVLARVGGEEFAVVVPRTDMHGGAALAE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
RIRAAVEEQIIPVGGGRETTIRVSLGVACMEAGSVPVLPHDLMEQADEALYRAKANGRNR
HHHHHHHHCEECCCCCCCEEEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCE
VELAAPAAEKGA
EEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]