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The map label for this gene is gltX

Identifier: 78357690

GI number: 78357690

Start: 2663020

End: 2664414

Strand: Reverse

Name: gltX

Synonym: Dde_2648

Alternate gene names: 78357690

Gene position: 2664414-2663020 (Counterclockwise)

Preceding gene: 78357691

Following gene: 78357686

Centisome position: 71.43

GC content: 60.79

Gene sequence:

>1395_bases
ATGAGCAAGGTTGTAACGCGATTTGCTCCCAGCCCCACGGGGCATCTGCACCTCGGCGGTGCACGCACCGCCGTGTTCAG
CTGGCTGCTGGCCCGCCATTTCGGCGGTGAATTTGTGCTGCGTATCGAAGATACCGACATGGAACGCTCGAAGCAGGAGT
ACACAGACTCCATTCTGGCTTCCATGGCATGGCTGGGGCTGGACTGGGACGGCGAGCCTGTCTACCAGAGCCGCCGTTTT
GACCTGTACAACGAGTACATCGACAGGCTGCTGGCAGAGGGCAAGGCCTACTGGTGCGAATGTTCGCCCGAAGAAGTGGA
AGCCATGCGTGAAACCGCGCGGGCCCGCGGGCTGAAGCCCAAATACAACGGCCGCTGCCGCGAGCGCGGCCTGCAGGGCG
GTGAAGGCCGCGTGGTGCGGCTGAAGGCGCCGCTTACCGGCAAGACGGTGTTTACCGATCTGGTAAAGGGCACCATAGCC
TTTGATAACAGCGAGCTGGACGACATGATCATCCGGCGGGCCGACGGTTCGCCCACATACAACCTTGCCGTGGTGGTGGA
CGATGCCACCATGGGCGTGACACATGTGCTGCGCGGCGACGACCATGTGAACAACACCCCCAAGCAGATTCTGCTGTACG
AAGCTCTGGGACTGCCCGTGCCGGAATTCGGCCATGTGCCCATGATTCTGGGGCCGGACAGACAGAAGCTTTCCAAGCGG
CACGGGGCCAAGGCGGTTATAGATTATAAAGAAACCGGCCTGCTGCCTCAGGCGCTTGTGAACTATCTGGTACGGCTCGG
CTGGTCATACGGAGATCAGGAAAAATTTTCGCTGGATGAGCTGATAGAGAAGTTCTCCACTGATAATCTGTCGCGTTCCG
CTGCGGGATTCGATCCGGAAAAGCTGCAGTGGCTTAACGGTCAGTACATCAGGGAAACTCCCGACGACCAGCTGGCCGGT
CTTGTGCGGCCTTTTCTGGCAGAAACGGGATACGGTCAGATTGATGCAGCTTTTCTGACTGTTGCGGCGGGCCTGTACAA
GGAACGCGCCCATGATCTTGTGGAGCTGGCAGCAGCCATGAAACCCGTGCTCTGTGCCGATGACGCGCTGGAGTTTGATG
CCAAAGCCGTGGAAAAGGCACTGACGGCTGAAGGCCGCGGGCATCTGGCTGCGTTGCGGCAGGCGTTTGCCGCCTGTGCT
GACTTTGACGGGCACACCGCCCATGAAGTGCTGCAGCGCTATGTGGCTGACAACGGGCTGAAATTCAAGGCCGTGGGGCC
GCCTTTGCGTGTGGCCCTGCTGGGGGCCATGGGCGGACCCGACCTTTCCGCCGTCATGGGCCTGCTGGGCAGGGAACGCA
CGCTGGCGCGCCTTGACCGTGCGGCGGCATTGTAA

Upstream 100 bases:

>100_bases
TTTCAGGCGGACATGGTGTTCGCCCTTTTACATATTTAGCCGCGGGGCGCGCCGCCCCGTCCAACGGCGCTCTGTCCGCA
GAGCGCACCAAGGAAGCATC

Downstream 100 bases:

>100_bases
TTCTGTTTTTGTGCGTCAATGAATACAGCGCCGTATCCTGATGGATACGGCGCTGCTTTTTTTGAGAAAGGAGGCTGTGC
GGCGGAGGGGAATTATCCGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSKVVTRFAPSPTGHLHLGGARTAVFSWLLARHFGGEFVLRIEDTDMERSKQEYTDSILASMAWLGLDWDGEPVYQSRRF
DLYNEYIDRLLAEGKAYWCECSPEEVEAMRETARARGLKPKYNGRCRERGLQGGEGRVVRLKAPLTGKTVFTDLVKGTIA
FDNSELDDMIIRRADGSPTYNLAVVVDDATMGVTHVLRGDDHVNNTPKQILLYEALGLPVPEFGHVPMILGPDRQKLSKR
HGAKAVIDYKETGLLPQALVNYLVRLGWSYGDQEKFSLDELIEKFSTDNLSRSAAGFDPEKLQWLNGQYIRETPDDQLAG
LVRPFLAETGYGQIDAAFLTVAAGLYKERAHDLVELAAAMKPVLCADDALEFDAKAVEKALTAEGRGHLAALRQAFAACA
DFDGHTAHEVLQRYVADNGLKFKAVGPPLRVALLGAMGGPDLSAVMGLLGRERTLARLDRAAAL

Sequences:

>Translated_464_residues
MSKVVTRFAPSPTGHLHLGGARTAVFSWLLARHFGGEFVLRIEDTDMERSKQEYTDSILASMAWLGLDWDGEPVYQSRRF
DLYNEYIDRLLAEGKAYWCECSPEEVEAMRETARARGLKPKYNGRCRERGLQGGEGRVVRLKAPLTGKTVFTDLVKGTIA
FDNSELDDMIIRRADGSPTYNLAVVVDDATMGVTHVLRGDDHVNNTPKQILLYEALGLPVPEFGHVPMILGPDRQKLSKR
HGAKAVIDYKETGLLPQALVNYLVRLGWSYGDQEKFSLDELIEKFSTDNLSRSAAGFDPEKLQWLNGQYIRETPDDQLAG
LVRPFLAETGYGQIDAAFLTVAAGLYKERAHDLVELAAAMKPVLCADDALEFDAKAVEKALTAEGRGHLAALRQAFAACA
DFDGHTAHEVLQRYVADNGLKFKAVGPPLRVALLGAMGGPDLSAVMGLLGRERTLARLDRAAAL
>Mature_463_residues
SKVVTRFAPSPTGHLHLGGARTAVFSWLLARHFGGEFVLRIEDTDMERSKQEYTDSILASMAWLGLDWDGEPVYQSRRFD
LYNEYIDRLLAEGKAYWCECSPEEVEAMRETARARGLKPKYNGRCRERGLQGGEGRVVRLKAPLTGKTVFTDLVKGTIAF
DNSELDDMIIRRADGSPTYNLAVVVDDATMGVTHVLRGDDHVNNTPKQILLYEALGLPVPEFGHVPMILGPDRQKLSKRH
GAKAVIDYKETGLLPQALVNYLVRLGWSYGDQEKFSLDELIEKFSTDNLSRSAAGFDPEKLQWLNGQYIRETPDDQLAGL
VRPFLAETGYGQIDAAFLTVAAGLYKERAHDLVELAAAMKPVLCADDALEFDAKAVEKALTAEGRGHLAALRQAFAACAD
FDGHTAHEVLQRYVADNGLKFKAVGPPLRVALLGAMGGPDLSAVMGLLGRERTLARLDRAAAL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=350, Percent_Identity=35.1428571428571, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI62241042, Length=229, Percent_Identity=27.5109170305677, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1788743, Length=461, Percent_Identity=47.5054229934924, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI226510925, Length=248, Percent_Identity=38.7096774193548, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1786895, Length=336, Percent_Identity=26.7857142857143, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71993394, Length=322, Percent_Identity=38.8198757763975, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17511007, Length=239, Percent_Identity=25.1046025104602, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324540, Length=491, Percent_Identity=34.0122199592668, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321192, Length=257, Percent_Identity=30.3501945525292, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324742, Length=134, Percent_Identity=35.8208955223881, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24665321, Length=490, Percent_Identity=32.6530612244898, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24649466, Length=225, Percent_Identity=28.4444444444444, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_DESDG (Q30Y02)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389139.1
- ProteinModelPortal:   Q30Y02
- SMR:   Q30Y02
- STRING:   Q30Y02
- GeneID:   3757671
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2648
- NMPDR:   fig|207559.3.peg.2903
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   DDES207559:DDE_2648-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51150; Mature: 51019

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVVTRFAPSPTGHLHLGGARTAVFSWLLARHFGGEFVLRIEDTDMERSKQEYTDSILA
CCCCHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHH
SMAWLGLDWDGEPVYQSRRFDLYNEYIDRLLAEGKAYWCECSPEEVEAMRETARARGLKP
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCC
KYNGRCRERGLQGGEGRVVRLKAPLTGKTVFTDLVKGTIAFDNSELDDMIIRRADGSPTY
CCCCHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCE
NLAVVVDDATMGVTHVLRGDDHVNNTPKQILLYEALGLPVPEFGHVPMILGPDRQKLSKR
EEEEEEECCHHCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHH
HGAKAVIDYKETGLLPQALVNYLVRLGWSYGDQEKFSLDELIEKFSTDNLSRSAAGFDPE
CCCCEEEECHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHH
KLQWLNGQYIRETPDDQLAGLVRPFLAETGYGQIDAAFLTVAAGLYKERAHDLVELAAAM
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KPVLCADDALEFDAKAVEKALTAEGRGHLAALRQAFAACADFDGHTAHEVLQRYVADNGL
CCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
KFKAVGPPLRVALLGAMGGPDLSAVMGLLGRERTLARLDRAAAL
EEEECCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVVTRFAPSPTGHLHLGGARTAVFSWLLARHFGGEFVLRIEDTDMERSKQEYTDSILA
CCCHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHH
SMAWLGLDWDGEPVYQSRRFDLYNEYIDRLLAEGKAYWCECSPEEVEAMRETARARGLKP
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCC
KYNGRCRERGLQGGEGRVVRLKAPLTGKTVFTDLVKGTIAFDNSELDDMIIRRADGSPTY
CCCCHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCE
NLAVVVDDATMGVTHVLRGDDHVNNTPKQILLYEALGLPVPEFGHVPMILGPDRQKLSKR
EEEEEEECCHHCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHH
HGAKAVIDYKETGLLPQALVNYLVRLGWSYGDQEKFSLDELIEKFSTDNLSRSAAGFDPE
CCCCEEEECHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHH
KLQWLNGQYIRETPDDQLAGLVRPFLAETGYGQIDAAFLTVAAGLYKERAHDLVELAAAM
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KPVLCADDALEFDAKAVEKALTAEGRGHLAALRQAFAACADFDGHTAHEVLQRYVADNGL
CCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
KFKAVGPPLRVALLGAMGGPDLSAVMGLLGRERTLARLDRAAAL
EEEECCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA