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The map label for this gene is argF

Identifier: 78357575

GI number: 78357575

Start: 2551615

End: 2552517

Strand: Reverse

Name: argF

Synonym: Dde_2532

Alternate gene names: 78357575

Gene position: 2552517-2551615 (Counterclockwise)

Preceding gene: 78357579

Following gene: 78357574

Centisome position: 68.43

GC content: 54.49

Gene sequence:

>903_bases
ATGACGAAGCACTTTACGCAAATTCGGGATCTCGGATATCAGGCGGCATGGGACGTACTGCGCCGCGCCAAGGAAATGAA
AGATTCCCGCCACAGGAGCACGCTGCTGGAAGGCAAAAGCGCCATCATGCTGTTTGAAAAAGCATCTACCCGCACGCGCG
TGTCTTTTGAAACGGCCGTGCATCAGCTGGGCGGCAAGACCATTTTCATGACTCCGGCCGAAAGTCAGCTGGGCCGTTCC
GAACCCCTGCGTGATACAGCCCGCGTTCTGTCCAGATACAACGATCTGATGATTGTAAGGACATTCGGACAGGAAAAAAT
AGACGAACTCGTTGAATACGGCAGCATTCCTGTTATCAATGCGCTGACGGACGAAGGGCACCCCTGTCAGGTGATGAGCG
ACATGCTGACCATATACGAGCGCACCCCTGATCTGGAAAAAGTGCGTGTGGCATGGGTGGGTGACGGCAACAACATGGCC
AACTCATGGATCGAAGCCGCGATTTTCTTCAAATTCGAGCTGTTCATGGCATTCCCCGAAGGCTACGAACCCGACAGAAA
CCTGCTGGCGCTTGCCATGCAGGCCGGTGCTAAAATTTTTCTCACGCATGATCCTGCCATGGCCGTGGACGGCGCTCATT
ATGTGAATACCGATGTATGGGCCTCCATGGGGCAGGAAGAAGAACAGAAAAAGCGCGAAAAGGCATTTGCAGGTTTCTGC
GTGGATGACGCGCTGATGGCAAAGGCTGCGCCCGATGCCCGATTCATGCACTGTCTGCCCGCGCACCGCGGTGAAGAAGT
GACGGAAGATGTTTTTGAAAGCCCTGCTTCCATTGTCTGGGATCAGGCGGAAAACCGCCTGCACATGCAGAAAGCCCTTA
TCGAGTGGGTGATGGAAGGCTAG

Upstream 100 bases:

>100_bases
CGGACTTTACTTTACAAATCCTTGGTTTTGGCCCTAAATGTTTCTCTTCGGCTGAGGGCTGACACCTGAACGCAGCAGTA
TTCATACAAAGGAACTGGCC

Downstream 100 bases:

>100_bases
GGCTGAACCTGCGCCGCCCGTTGCTGCGCAGATGACGGTTGTACCACCACGGGGCGCGGGGCGCCCCGTTTTCAGTAAGG
GCGCTTCGCGCGTCCGGCGG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTKHFTQIRDLGYQAAWDVLRRAKEMKDSRHRSTLLEGKSAIMLFEKASTRTRVSFETAVHQLGGKTIFMTPAESQLGRS
EPLRDTARVLSRYNDLMIVRTFGQEKIDELVEYGSIPVINALTDEGHPCQVMSDMLTIYERTPDLEKVRVAWVGDGNNMA
NSWIEAAIFFKFELFMAFPEGYEPDRNLLALAMQAGAKIFLTHDPAMAVDGAHYVNTDVWASMGQEEEQKKREKAFAGFC
VDDALMAKAAPDARFMHCLPAHRGEEVTEDVFESPASIVWDQAENRLHMQKALIEWVMEG

Sequences:

>Translated_300_residues
MTKHFTQIRDLGYQAAWDVLRRAKEMKDSRHRSTLLEGKSAIMLFEKASTRTRVSFETAVHQLGGKTIFMTPAESQLGRS
EPLRDTARVLSRYNDLMIVRTFGQEKIDELVEYGSIPVINALTDEGHPCQVMSDMLTIYERTPDLEKVRVAWVGDGNNMA
NSWIEAAIFFKFELFMAFPEGYEPDRNLLALAMQAGAKIFLTHDPAMAVDGAHYVNTDVWASMGQEEEQKKREKAFAGFC
VDDALMAKAAPDARFMHCLPAHRGEEVTEDVFESPASIVWDQAENRLHMQKALIEWVMEG
>Mature_299_residues
TKHFTQIRDLGYQAAWDVLRRAKEMKDSRHRSTLLEGKSAIMLFEKASTRTRVSFETAVHQLGGKTIFMTPAESQLGRSE
PLRDTARVLSRYNDLMIVRTFGQEKIDELVEYGSIPVINALTDEGHPCQVMSDMLTIYERTPDLEKVRVAWVGDGNNMAN
SWIEAAIFFKFELFMAFPEGYEPDRNLLALAMQAGAKIFLTHDPAMAVDGAHYVNTDVWASMGQEEEQKKREKAFAGFCV
DDALMAKAAPDARFMHCLPAHRGEEVTEDVFESPASIVWDQAENRLHMQKALIEWVMEG

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=256, Percent_Identity=46.484375, Blast_Score=224, Evalue=6e-59,
Organism=Homo sapiens, GI18105007, Length=269, Percent_Identity=25.2788104089219, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1790703, Length=322, Percent_Identity=39.1304347826087, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI1786469, Length=322, Percent_Identity=38.5093167701863, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=28.8025889967638, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI193204318, Length=125, Percent_Identity=34.4, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=317, Percent_Identity=33.7539432176656, Blast_Score=179, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6322331, Length=271, Percent_Identity=25.0922509225092, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24642586, Length=311, Percent_Identity=24.7588424437299, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_DESDG (Q30YB7)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389024.1
- HSSP:   P96108
- ProteinModelPortal:   Q30YB7
- SMR:   Q30YB7
- STRING:   Q30YB7
- GeneID:   3757549
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2532
- NMPDR:   fig|207559.3.peg.3470
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   KHETLLE
- ProtClustDB:   PRK00779
- BioCyc:   DDES207559:DDE_2532-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34028; Mature: 33897

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 100-100 BINDING 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHFTQIRDLGYQAAWDVLRRAKEMKDSRHRSTLLEGKSAIMLFEKASTRTRVSFETAV
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
HQLGGKTIFMTPAESQLGRSEPLRDTARVLSRYNDLMIVRTFGQEKIDELVEYGSIPVIN
HHHCCCEEEECCCHHHCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCEEE
ALTDEGHPCQVMSDMLTIYERTPDLEKVRVAWVGDGNNMANSWIEAAIFFKFELFMAFPE
ECCCCCCCHHHHHHHHHHHHCCCCHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHEECCC
GYEPDRNLLALAMQAGAKIFLTHDPAMAVDGAHYVNTDVWASMGQEEEQKKREKAFAGFC
CCCCCHHHHHHHHHCCCEEEEECCCCCEECCCHHCCHHHHHHCCCHHHHHHHHHHHHCCC
VDDALMAKAAPDARFMHCLPAHRGEEVTEDVFESPASIVWDQAENRLHMQKALIEWVMEG
HHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKHFTQIRDLGYQAAWDVLRRAKEMKDSRHRSTLLEGKSAIMLFEKASTRTRVSFETAV
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
HQLGGKTIFMTPAESQLGRSEPLRDTARVLSRYNDLMIVRTFGQEKIDELVEYGSIPVIN
HHHCCCEEEECCCHHHCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCEEE
ALTDEGHPCQVMSDMLTIYERTPDLEKVRVAWVGDGNNMANSWIEAAIFFKFELFMAFPE
ECCCCCCCHHHHHHHHHHHHCCCCHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHEECCC
GYEPDRNLLALAMQAGAKIFLTHDPAMAVDGAHYVNTDVWASMGQEEEQKKREKAFAGFC
CCCCCHHHHHHHHHCCCEEEEECCCCCEECCCHHCCHHHHHHCCCHHHHHHHHHHHHCCC
VDDALMAKAAPDARFMHCLPAHRGEEVTEDVFESPASIVWDQAENRLHMQKALIEWVMEG
HHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA