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The map label for this gene is greA [H]

Identifier: 78357555

GI number: 78357555

Start: 2533456

End: 2534163

Strand: Reverse

Name: greA [H]

Synonym: Dde_2512

Alternate gene names: 78357555

Gene position: 2534163-2533456 (Counterclockwise)

Preceding gene: 78357559

Following gene: 78357554

Centisome position: 67.94

GC content: 51.84

Gene sequence:

>708_bases
ATGACATTTCTATTGTCCCAGACAGGGGTGACAATATCATTGTCCGGTAACATATTTTGCGAACGGGGCTTGACCTCCGG
TGAACTCTGTTCTACCCCTCAGGTTGCCAACACCTGTTTCGGACAGTGTCGAACAGCCTCGCGACCCATGGGCCGCGTGG
CGTTTTTTCTTATTGTCGTTGGCAAAAAACTATCGAGAAATGTCAAGGAATCAAACATGAGCAGCATCCCGATTTCAGTT
GAAGGCTTTGAACTGGTTAAACGAGATCTTGAAAGGCTGAAGCAGGAACGTCCCGCAGTGATTCAGGCCATCAAGGAAGC
ACGCGAAGAAGGCGACCTGAGCGAAAACGCAGGCTATGATGCCGCCCGTGAGCGTCAGGGCATGCTTGAAGCGCGTATCG
GCAACATCGAATCCCGCATGGCCCGCTTCAATGTCATTGATATGAGAACGCTTGGCGGCGAGCGTGTGACCTATGGTGCC
ACCATAGAGCTGGAAGACCTCGAAACCGGTGAAATGCGGGTGTATACTCTGGTGGGTCCCGACGAGACTGACTATGTGAA
AGGCAGCATCTCGGTTCTTTCTCCTGTTGCCAAAGCTGTTCTGGGCAAAGAGGAAGGCGACGAAGTTGTGGTGGACGCCC
CGAGAGGAAAAATACATTACGAAATCGTTTCTGTAAGCTTCAACGGCCCTGTCATCACCCTCGAATAG

Upstream 100 bases:

>100_bases
CCTCCCGAACCGTGAACGCTCCGGCCACTGCCTGCTTGATCACAAACGCTCGCCGCGCTTCCTCTGCCTTCATGAGTACG
TCACCACGTGTCTTCATGGG

Downstream 100 bases:

>100_bases
TTTTACAGGCCAGTCTGGATGTGCTCCTGTCGGCGCCACCGGGCGGCCAGTACCTCCGGTGACACCAGACAGCCAAGACA
AGCTCTGTAAGCCCCTGCCG

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTFLLSQTGVTISLSGNIFCERGLTSGELCSTPQVANTCFGQCRTASRPMGRVAFFLIVVGKKLSRNVKESNMSSIPISV
EGFELVKRDLERLKQERPAVIQAIKEAREEGDLSENAGYDAARERQGMLEARIGNIESRMARFNVIDMRTLGGERVTYGA
TIELEDLETGEMRVYTLVGPDETDYVKGSISVLSPVAKAVLGKEEGDEVVVDAPRGKIHYEIVSVSFNGPVITLE

Sequences:

>Translated_235_residues
MTFLLSQTGVTISLSGNIFCERGLTSGELCSTPQVANTCFGQCRTASRPMGRVAFFLIVVGKKLSRNVKESNMSSIPISV
EGFELVKRDLERLKQERPAVIQAIKEAREEGDLSENAGYDAARERQGMLEARIGNIESRMARFNVIDMRTLGGERVTYGA
TIELEDLETGEMRVYTLVGPDETDYVKGSISVLSPVAKAVLGKEEGDEVVVDAPRGKIHYEIVSVSFNGPVITLE
>Mature_234_residues
TFLLSQTGVTISLSGNIFCERGLTSGELCSTPQVANTCFGQCRTASRPMGRVAFFLIVVGKKLSRNVKESNMSSIPISVE
GFELVKRDLERLKQERPAVIQAIKEAREEGDLSENAGYDAARERQGMLEARIGNIESRMARFNVIDMRTLGGERVTYGAT
IELEDLETGEMRVYTLVGPDETDYVKGSISVLSPVAKAVLGKEEGDEVVVDAPRGKIHYEIVSVSFNGPVITLE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=43.312101910828, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI87082261, Length=159, Percent_Identity=30.8176100628931, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 25727; Mature: 25596

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLLSQTGVTISLSGNIFCERGLTSGELCSTPQVANTCFGQCRTASRPMGRVAFFLIVV
CEEEEECCCEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
GKKLSRNVKESNMSSIPISVEGFELVKRDLERLKQERPAVIQAIKEAREEGDLSENAGYD
HHHHHCCHHHCCCCCCCEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCC
AARERQGMLEARIGNIESRMARFNVIDMRTLGGERVTYGATIELEDLETGEMRVYTLVGP
HHHHHHCHHHHHHCCHHHHHHHHEEEEEEECCCCEEEEEEEEEECCCCCCCEEEEEEECC
DETDYVKGSISVLSPVAKAVLGKEEGDEVVVDAPRGKIHYEIVSVSFNGPVITLE
CCCCEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEEECCCEEEEC
>Mature Secondary Structure 
TFLLSQTGVTISLSGNIFCERGLTSGELCSTPQVANTCFGQCRTASRPMGRVAFFLIVV
EEEEECCCEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
GKKLSRNVKESNMSSIPISVEGFELVKRDLERLKQERPAVIQAIKEAREEGDLSENAGYD
HHHHHCCHHHCCCCCCCEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCC
AARERQGMLEARIGNIESRMARFNVIDMRTLGGERVTYGATIELEDLETGEMRVYTLVGP
HHHHHHCHHHHHHCCHHHHHHHHEEEEEEECCCCEEEEEEEEEECCCCCCCEEEEEEECC
DETDYVKGSISVLSPVAKAVLGKEEGDEVVVDAPRGKIHYEIVSVSFNGPVITLE
CCCCEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA