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The map label for this gene is ribE [H]

Identifier: 78357478

GI number: 78357478

Start: 2456154

End: 2456828

Strand: Direct

Name: ribE [H]

Synonym: Dde_2435

Alternate gene names: 78357478

Gene position: 2456154-2456828 (Clockwise)

Preceding gene: 304570589

Following gene: 78357479

Centisome position: 65.84

GC content: 58.52

Gene sequence:

>675_bases
ATGTTTACCGGAATAATCAACGGACAGGGCGAAATCCTGTCGGCAGACAACAGGGGCAGTGAAACCCGTTTCCGCATCCG
GCCCCGCTTTGCGCTGACGGACTATGTCACCGGCGAATCCATCGCCGTGAACGGCGTATGTCTTACCGTGGAAACCTATG
CGGACAGCTGGTTCAGCGCATACGCATCTGCGGAAACCCTGTCCTGCTCCAACCTTGGAGCCCTGCACACCGGCGCTGCC
GTTAATCTGGAGCGCGCGCTTGCAGTGGGTGACAGGCTGGGCGGGCACATTGTCAGCGGACATGTGGACTGTCTGGCCAC
GGTGCAGTCGGTGCAGCCTGCGGGGCTTTCGCGCGTATACCGGCTCGGCTTTCCGCAGGAATTCGGGATTCAGGTGGTGG
ATAAAGGCTCCGTAACTCTTGACGGTATCAGCCTTACGGTTAATCATTGCGGGCCGGATTTTCTGGAAGTGAACATCATT
CCCGAAACGCAAAAGGTGACAACCATCGCGCACTGGAAGCCCGGAACCCGCGTAAACATGGAAACCGACATCATCGGCAA
ATATGTTCAGCGCATGCTCACCCCGTGGAGCTCCGCAGACAAAGGCGGCGCAACCGCAGGCGACAGCCGGGCCGGCACTC
TGACAATGGACTTTCTGCGCGAACACGGCTTTTAA

Upstream 100 bases:

>100_bases
CACCGTCCACCATGGAACAGGCGCTGGGCCTGCGCATCTGTAATGTCGCCCGCAGCGGCGAAGACGTTATCCTGACCTTG
CGCCCGAGGAAATAACACCT

Downstream 100 bases:

>100_bases
CCGTAAAACGCCGCAAACCGCCGGCGGCACGACCGCCGGAGGATACCGCAACTTACCGAGAGGTTCCCAGATATGCCTAT
GTGCACGGCCGAAGAGGCCA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MFTGIINGQGEILSADNRGSETRFRIRPRFALTDYVTGESIAVNGVCLTVETYADSWFSAYASAETLSCSNLGALHTGAA
VNLERALAVGDRLGGHIVSGHVDCLATVQSVQPAGLSRVYRLGFPQEFGIQVVDKGSVTLDGISLTVNHCGPDFLEVNII
PETQKVTTIAHWKPGTRVNMETDIIGKYVQRMLTPWSSADKGGATAGDSRAGTLTMDFLREHGF

Sequences:

>Translated_224_residues
MFTGIINGQGEILSADNRGSETRFRIRPRFALTDYVTGESIAVNGVCLTVETYADSWFSAYASAETLSCSNLGALHTGAA
VNLERALAVGDRLGGHIVSGHVDCLATVQSVQPAGLSRVYRLGFPQEFGIQVVDKGSVTLDGISLTVNHCGPDFLEVNII
PETQKVTTIAHWKPGTRVNMETDIIGKYVQRMLTPWSSADKGGATAGDSRAGTLTMDFLREHGF
>Mature_224_residues
MFTGIINGQGEILSADNRGSETRFRIRPRFALTDYVTGESIAVNGVCLTVETYADSWFSAYASAETLSCSNLGALHTGAA
VNLERALAVGDRLGGHIVSGHVDCLATVQSVQPAGLSRVYRLGFPQEFGIQVVDKGSVTLDGISLTVNHCGPDFLEVNII
PETQKVTTIAHWKPGTRVNMETDIIGKYVQRMLTPWSSADKGGATAGDSRAGTLTMDFLREHGF

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=185, Percent_Identity=37.2972972972973, Blast_Score=105, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319733, Length=237, Percent_Identity=34.5991561181435, Blast_Score=111, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 24023; Mature: 24023

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIINGQGEILSADNRGSETRFRIRPRFALTDYVTGESIAVNGVCLTVETYADSWFSA
CCEEEECCCCCEEECCCCCCCCEEEEECCEEEEEEECCCEEEECEEEEEEEHHHHHHHHH
YASAETLSCSNLGALHTGAAVNLERALAVGDRLGGHIVSGHVDCLATVQSVQPAGLSRVY
HCCCCEECCCCCCCCCCCCEECHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCHHHHH
RLGFPQEFGIQVVDKGSVTLDGISLTVNHCGPDFLEVNIIPETQKVTTIAHWKPGTRVNM
HCCCCHHHCEEEEECCCEEEEEEEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCEECC
ETDIIGKYVQRMLTPWSSADKGGATAGDSRAGTLTMDFLREHGF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHCCC
>Mature Secondary Structure
MFTGIINGQGEILSADNRGSETRFRIRPRFALTDYVTGESIAVNGVCLTVETYADSWFSA
CCEEEECCCCCEEECCCCCCCCEEEEECCEEEEEEECCCEEEECEEEEEEEHHHHHHHHH
YASAETLSCSNLGALHTGAAVNLERALAVGDRLGGHIVSGHVDCLATVQSVQPAGLSRVY
HCCCCEECCCCCCCCCCCCEECHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCHHHHH
RLGFPQEFGIQVVDKGSVTLDGISLTVNHCGPDFLEVNIIPETQKVTTIAHWKPGTRVNM
HCCCCHHHCEEEEECCCEEEEEEEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCEECC
ETDIIGKYVQRMLTPWSSADKGGATAGDSRAGTLTMDFLREHGF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]