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The map label for this gene is rlmH

Identifier: 78357334

GI number: 78357334

Start: 2312179

End: 2312646

Strand: Direct

Name: rlmH

Synonym: Dde_2291

Alternate gene names: 78357334

Gene position: 2312179-2312646 (Clockwise)

Preceding gene: 78357333

Following gene: 78357335

Centisome position: 61.98

GC content: 58.33

Gene sequence:

>468_bases
ATGAGAAGCATACGCATCATAGCCGTGGGCAGGTTGAAAACAGCACACTGGAAGACGGCTGCGGAACACTATCTTACCCG
TCTTACCAGAGCATTCAAGGTTGAAGAAACTATTATCAAGGACGGAAACGCCGCCCTGCCGCCGCTGGAGCGTAACGCGC
AGGAAGGCGCACGCATTGTTGCGGCGCTGACGCCCGCCGATCTGGCTGTCTGCATGGATGAAACCGGCAGGCAGTTCACT
TCGCAGCAGTTTTCGGCCTTTCTGACTCCCATGTGGGAAAACGCCAACCTGCGCCCGTGCTTTATCATCGGAGGAGCCTA
CGGCCTTTCGGATGAAGTACGCGGCAAAGCGCGGCACACCATGGCCCTTTCCGCCATGACCTTTCCGCACGAGATGGCGC
GCGTGGTGCTGTACGAACAGCTGTACAGGGCAGACGCCATACTGCGGGGCACTCCCTACCATCACTAG

Upstream 100 bases:

>100_bases
TGCCCGCCACCGGTGAACGGCTTGAGGCTTTCGGTCTTGCAGTATACAAAGCGCCCATGCGCATTAACAACACAGCATCA
TATTTCTGCCGGAGCGGCAT

Downstream 100 bases:

>100_bases
CTGCGCAGGGCGGCGCCGCCGCACCGGCCAACCGCAACCGCAAAGGATCAACCATGCTTGAATTCAGCTATCTGGAAAAA
CTGGAAGAATACATGAAGTC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MRSIRIIAVGRLKTAHWKTAAEHYLTRLTRAFKVEETIIKDGNAALPPLERNAQEGARIVAALTPADLAVCMDETGRQFT
SQQFSAFLTPMWENANLRPCFIIGGAYGLSDEVRGKARHTMALSAMTFPHEMARVVLYEQLYRADAILRGTPYHH

Sequences:

>Translated_155_residues
MRSIRIIAVGRLKTAHWKTAAEHYLTRLTRAFKVEETIIKDGNAALPPLERNAQEGARIVAALTPADLAVCMDETGRQFT
SQQFSAFLTPMWENANLRPCFIIGGAYGLSDEVRGKARHTMALSAMTFPHEMARVVLYEQLYRADAILRGTPYHH
>Mature_155_residues
MRSIRIIAVGRLKTAHWKTAAEHYLTRLTRAFKVEETIIKDGNAALPPLERNAQEGARIVAALTPADLAVCMDETGRQFT
SQQFSAFLTPMWENANLRPCFIIGGAYGLSDEVRGKARHTMALSAMTFPHEMARVVLYEQLYRADAILRGTPYHH

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=160, Percent_Identity=30.625, Blast_Score=64, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_DESDG (Q30Z08)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388783.1
- ProteinModelPortal:   Q30Z08
- SMR:   Q30Z08
- STRING:   Q30Z08
- GeneID:   3757302
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2291
- NMPDR:   fig|207559.3.peg.2103
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   NISHYKI
- ProtClustDB:   CLSK705132
- BioCyc:   DDES207559:DDE_2291-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17399; Mature: 17399

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIRIIAVGRLKTAHWKTAAEHYLTRLTRAFKVEETIIKDGNAALPPLERNAQEGARIV
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEE
AALTPADLAVCMDETGRQFTSQQFSAFLTPMWENANLRPCFIIGGAYGLSDEVRGKARHT
EEECCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCHHHHH
MALSAMTFPHEMARVVLYEQLYRADAILRGTPYHH
HHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRSIRIIAVGRLKTAHWKTAAEHYLTRLTRAFKVEETIIKDGNAALPPLERNAQEGARIV
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEE
AALTPADLAVCMDETGRQFTSQQFSAFLTPMWENANLRPCFIIGGAYGLSDEVRGKARHT
EEECCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCHHHHH
MALSAMTFPHEMARVVLYEQLYRADAILRGTPYHH
HHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA