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The map label for this gene is hydA [H]

Identifier: 78357324

GI number: 78357324

Start: 2301312

End: 2302631

Strand: Reverse

Name: hydA [H]

Synonym: Dde_2281

Alternate gene names: 78357324

Gene position: 2302631-2301312 (Counterclockwise)

Preceding gene: 78357325

Following gene: 78357323

Centisome position: 61.73

GC content: 58.48

Gene sequence:

>1320_bases
ATGGCTGGATGCAAAGCACAGCACCCGCCCGCAGCCTATCTGGCAGGGTTGGAAGTACCTGCCGCAGGCTCCGAGGTAAC
CATGGAAGGTGTTCGTTATAAAATGAACGCCCCCAAGGATGTAGACCCCGCAACCATCCGGTTTGTGGAGGTCGACCACG
ACAAGTGCATGGCGTGCGGCGAGTGTGAGTATCACTGTCCCACAGGCGTCATGCAGGAAGTGACCGAAGACGGCTATCGC
GGTGTCGTGGACCCCGTGGCGTGCGTCAACTGCGGGCAGTGTCTTGCAAACTGTCCCTTCGGCGCCATCCATGAAGAGGT
GTCGTTTGTAGGCGAATTGTACGAAAAACTCAAGGATCCTGACACGGTTGTCGTATCCATGCCTGCTCCCGCCGTGCGCT
ATGCGCTGGGTGAATGCTTCGGGCTGCCTACGGGTACGTATGTGGGCGGGCAGATGCATGCCGCCCTGCGCCGTCTGGGC
TTTAATCTGGTGTGGGATACGGAATGGACGGCTGACGTGACCATCATGGAAGAGGGCACCGAACTGCTGGAACGCGTGAA
GCACGGCAATATGCCGCTGCCGCAGTTCACTTCCTGCTGCCCCGGGTGGATCAAGTTTGCCGAAACGTTCTACCCCGATC
TGGAAAAGCACCTTTCCACCTGTAAATCCCCCATTGCCATGATCGGCCCGCTTGCCAAAACGTATGGTGCGCAGGAGGCC
GGGGTACCCGCCAAAAAAATGTACACCGTGTCCATCATGCCCTGCATAGCCAAAAAGTTTGAAGGTATGCGGCCTGAAAT
GAATGCCAGCGGGTACAGAGACATCGACGCCACCATCACCACGCGTGAGCTGGCATGGATGATTAAAAAAGCGGGCATCG
ACTTCACTTCGCTGCCTTCCGAAGAACCGGATCCCGCGCTGGGCATGTCCACTGGTGCTGCCACCATTTTCTGCACCAGC
GGCGGTGTAATGGAAGCTGCCCTGCGTCTGGCGTATGAAGCGCTGTCCGGCGGCACCCTTGCCGACCCCGACATCAAGGT
GGTGCGCACGCATGAGGGTATAAATACGGCAGAAGTGCCCGTACCCAACTTCGGCACCGTGAAAGTGGCCGTGGCAAGCG
GTCTGGACAATGCCGCCAAGCTGTGCGAGGAAGTGCGCGCAGGCAAATCGCCTTACCACTTCATCGAGGTGATGACGTGT
CCCGGCGGCTGCGTAAACGGCGGCGGGCAACCGCTTGAACCGGGCATGCTGCAGTCTTCCCTTTTCAAGAGCACTATCAC
CAAGATCAACCGTCGGTTTACCAGACGGTCTGTGGCATAG

Upstream 100 bases:

>100_bases
AGCACTGAAGCAGTGCAAGTATTACTAATGTCAATTTAGTAACACTTTTTCCATTGCTCTTGTCTATAAGAGCAGGCAAC
ACTGAAGCAAGGAGAAGGTT

Downstream 100 bases:

>100_bases
GAGGATGGCATGAGCAGATTAGGTACGGTATCCCGTCGCGGATTTATCAAGCTCGCCGGCTTTGCGGCCGGTTATGCCGT
TTTCGGCTTTAATATGGCAC

Product: ferredoxin hydrogenase

Products: NA

Alternate protein names: Fe hydrogenlyase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MAGCKAQHPPAAYLAGLEVPAAGSEVTMEGVRYKMNAPKDVDPATIRFVEVDHDKCMACGECEYHCPTGVMQEVTEDGYR
GVVDPVACVNCGQCLANCPFGAIHEEVSFVGELYEKLKDPDTVVVSMPAPAVRYALGECFGLPTGTYVGGQMHAALRRLG
FNLVWDTEWTADVTIMEEGTELLERVKHGNMPLPQFTSCCPGWIKFAETFYPDLEKHLSTCKSPIAMIGPLAKTYGAQEA
GVPAKKMYTVSIMPCIAKKFEGMRPEMNASGYRDIDATITTRELAWMIKKAGIDFTSLPSEEPDPALGMSTGAATIFCTS
GGVMEAALRLAYEALSGGTLADPDIKVVRTHEGINTAEVPVPNFGTVKVAVASGLDNAAKLCEEVRAGKSPYHFIEVMTC
PGGCVNGGGQPLEPGMLQSSLFKSTITKINRRFTRRSVA

Sequences:

>Translated_439_residues
MAGCKAQHPPAAYLAGLEVPAAGSEVTMEGVRYKMNAPKDVDPATIRFVEVDHDKCMACGECEYHCPTGVMQEVTEDGYR
GVVDPVACVNCGQCLANCPFGAIHEEVSFVGELYEKLKDPDTVVVSMPAPAVRYALGECFGLPTGTYVGGQMHAALRRLG
FNLVWDTEWTADVTIMEEGTELLERVKHGNMPLPQFTSCCPGWIKFAETFYPDLEKHLSTCKSPIAMIGPLAKTYGAQEA
GVPAKKMYTVSIMPCIAKKFEGMRPEMNASGYRDIDATITTRELAWMIKKAGIDFTSLPSEEPDPALGMSTGAATIFCTS
GGVMEAALRLAYEALSGGTLADPDIKVVRTHEGINTAEVPVPNFGTVKVAVASGLDNAAKLCEEVRAGKSPYHFIEVMTC
PGGCVNGGGQPLEPGMLQSSLFKSTITKINRRFTRRSVA
>Mature_438_residues
AGCKAQHPPAAYLAGLEVPAAGSEVTMEGVRYKMNAPKDVDPATIRFVEVDHDKCMACGECEYHCPTGVMQEVTEDGYRG
VVDPVACVNCGQCLANCPFGAIHEEVSFVGELYEKLKDPDTVVVSMPAPAVRYALGECFGLPTGTYVGGQMHAALRRLGF
NLVWDTEWTADVTIMEEGTELLERVKHGNMPLPQFTSCCPGWIKFAETFYPDLEKHLSTCKSPIAMIGPLAKTYGAQEAG
VPAKKMYTVSIMPCIAKKFEGMRPEMNASGYRDIDATITTRELAWMIKKAGIDFTSLPSEEPDPALGMSTGAATIFCTSG
GVMEAALRLAYEALSGGTLADPDIKVVRTHEGINTAEVPVPNFGTVKVAVASGLDNAAKLCEEVRAGKSPYHFIEVMTCP
GGCVNGGGQPLEPGMLQSSLFKSTITKINRRFTRRSVA

Specific function: May be involved in hydrogen uptake for the reduction of sulfate to hydrogen sulfide in an electron transport chain. Cytochrome c3 is likely to be the physiological electron carrier for the enzyme [H]

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI11968051, Length=349, Percent_Identity=32.378223495702, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI84452151, Length=274, Percent_Identity=29.5620437956204, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI6912524, Length=274, Percent_Identity=29.5620437956204, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI134284357, Length=274, Percent_Identity=29.5620437956204, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI14165461, Length=310, Percent_Identity=28.7096774193548, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71995015, Length=345, Percent_Identity=27.536231884058, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324089, Length=401, Percent_Identity=22.9426433915212, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI116007470, Length=274, Percent_Identity=30.2919708029197, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI116007466, Length=274, Percent_Identity=30.2919708029197, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI116007464, Length=274, Percent_Identity=30.2919708029197, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI116007468, Length=274, Percent_Identity=30.2919708029197, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI116007462, Length=229, Percent_Identity=31.4410480349345, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR013352 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF00037 Fer4 [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 47189; Mature: 47058

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGCKAQHPPAAYLAGLEVPAAGSEVTMEGVRYKMNAPKDVDPATIRFVEVDHDKCMACG
CCCCCCCCCCHHHHCCCCCCCCCCCEECCCEEEECCCCCCCCCCEEEEEEECCCCCCCCC
ECEYHCPTGVMQEVTEDGYRGVVDPVACVNCGQCLANCPFGAIHEEVSFVGELYEKLKDP
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
DTVVVSMPAPAVRYALGECFGLPTGTYVGGQMHAALRRLGFNLVWDTEWTADVTIMEEGT
CEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCCCEEEEEHHHHH
ELLERVKHGNMPLPQFTSCCPGWIKFAETFYPDLEKHLSTCKSPIAMIGPLAKTYGAQEA
HHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
GVPAKKMYTVSIMPCIAKKFEGMRPEMNASGYRDIDATITTRELAWMIKKAGIDFTSLPS
CCCHHHEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
EEPDPALGMSTGAATIFCTSGGVMEAALRLAYEALSGGTLADPDIKVVRTHEGINTAEVP
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCC
VPNFGTVKVAVASGLDNAAKLCEEVRAGKSPYHFIEVMTCPGGCVNGGGQPLEPGMLQSS
CCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHH
LFKSTITKINRRFTRRSVA
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGCKAQHPPAAYLAGLEVPAAGSEVTMEGVRYKMNAPKDVDPATIRFVEVDHDKCMACG
CCCCCCCCCHHHHCCCCCCCCCCCEECCCEEEECCCCCCCCCCEEEEEEECCCCCCCCC
ECEYHCPTGVMQEVTEDGYRGVVDPVACVNCGQCLANCPFGAIHEEVSFVGELYEKLKDP
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
DTVVVSMPAPAVRYALGECFGLPTGTYVGGQMHAALRRLGFNLVWDTEWTADVTIMEEGT
CEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCCCEEEEEHHHHH
ELLERVKHGNMPLPQFTSCCPGWIKFAETFYPDLEKHLSTCKSPIAMIGPLAKTYGAQEA
HHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
GVPAKKMYTVSIMPCIAKKFEGMRPEMNASGYRDIDATITTRELAWMIKKAGIDFTSLPS
CCCHHHEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
EEPDPALGMSTGAATIFCTSGGVMEAALRLAYEALSGGTLADPDIKVVRTHEGINTAEVP
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCC
VPNFGTVKVAVASGLDNAAKLCEEVRAGKSPYHFIEVMTCPGGCVNGGGQPLEPGMLQSS
CCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHH
LFKSTITKINRRFTRRSVA
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3888621; 3322275; 11456963; 10368269; 11456758 [H]